High performance liquid chromatography-tandem mass spectrometry method for detecting residual quantity of asomate in fruits and vegetables

Abstract

The invention relates to a high performance liquid chromatography-tandem mass spectrometry method for detecting the residual quantity of asomate in fruits and vegetables. The method comprises the following steps: preparing a standard working solution; extraction: preparing a fruit and vegetable sample to be detected into homogenate, homogenizing, and then centrifugally layering; purification: taking an upper organic phase of the layered solution, putting the upper organic phase into a centrifuge tube, carrying out vortex oscillation, centrifuging, taking supernate, carrying out nitrogen blowing concentration, carrying out constant-volume vortex mixing uniformly, taking a supernatant, and passing through a membrane for detection; preparing a matrix standard working solution, namely extracting and purifying the blank fruit and vegetable sample, and adding the standard working solution to obtain an asomate matrix standard working solution; and injecting the asomate matrix standard workingsolution and the to-be-detected sample solution into a liquid chromatograph, and detecting by a tandem mass spectrometer to obtain the residual quantity of asomate in the to-be-detected fruit and vegetable sample. According to the high performance liquid chromatography tandem mass spectrometry for detecting the residual quantity of the asomate in the fruits and the vegetables, the pretreatment method is simple, the purification effect is good, and the problems of much external interference and low sensitivity in asomate detection by liquid chromatography can be effectively solved.

Description

technical field [0001] The invention relates to the technical field of pesticide residue detection, in particular to a method for determining the residual amount of thirasine in fruits and vegetables by using high performance liquid chromatography tandem mass spectrometry. Background technique [0002] Arsone is a dithiocarbamate fungicide with strong antibacterial properties and long-term effectiveness. It has special effects on apple rot and can also prevent and control cucumber powdery mildew, rust downy mildew, pepper anthracnose, pear black It also has control effects on various diseases of crops such as powdery mildew of grapes, wheat, peas, and apple trees, rice blast, eggplant downy mildew, and hawthorn dieback. Arsine is moderately toxic and can be absorbed through the respiratory tract, gastrointestinal tract and skin. It takes a long time to excrete out of the body, and it will also be transformed into inorganic trivalent arsenic and its derivatives that may be mo...

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Problems solved by technology

[0004] The purpose of the present invention is to provide a high-performance liquid chromatography tandem mass spectrometry method for detecting residual amounts of thiramane in fruits and vegetables. The pretreatment method is simple and the purification effect is good, which can effectively solve the problem of external interference and sensitivity in the detection of thiramane by liquid chromatography. not high problem

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