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Method for detecting ethyl urethane content of cigarette sidestream smoke via LC-MS/MS combination

A technology of urethane and sidestream smoke, which is applied in the field of tobacco and chemistry, can solve the problems of small interference components, complex composition of sidestream smoke, etc., and achieve the effect of improving sensitivity

Active Publication Date: 2013-11-13
CHINA TOBACCO JIANGSU INDAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] As the public pays increasing attention to smoking and health issues, the harmful components of cigarette smoke—especially the content of ethyl carbamate—become the focus of attention. However, due to the relatively simple composition of the matrix in the above patent method, the interference components However, the composition of sidestream smoke is complex, with a wide variety of components, more and more complex detection interference factors, and serious matrix interference. Can the detection method established by the food industry be applicable to the detection of urethane in sidestream smoke? The determination is not yet known, and there are no literature reports and patents on the detection of ethyl carbamate in cigarette sidestream smoke at home and abroad

Method used

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  • Method for detecting ethyl urethane content of cigarette sidestream smoke via LC-MS/MS combination
  • Method for detecting ethyl urethane content of cigarette sidestream smoke via LC-MS/MS combination
  • Method for detecting ethyl urethane content of cigarette sidestream smoke via LC-MS/MS combination

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] A method for LC-MS / MS combined detection of urethane content in cigarette sidestream smoke, comprising the steps of:

[0048] Sample collection: equilibrate the cigarettes at (22±1)°C and relative humidity (60±3)% for 48 hours, then determine and mark the length of the butt of the smoked cigarettes, according to the sidestream smoke smoking method in YC / T185 , adjust the suction flow rate of the sidestream smoking machine to 3000mL / min, smoke 3 cigarettes, and use the fishtail hood and glass fiber filter to capture the total particulate matter in the sidestream smoke.

[0049] Extraction of filter: Put the filter with particulate matter of sidestream smoke from 3 cigarettes into a 100mL conical flask, add 10mL of ethanol and 2.00mL of secondary internal standard solution, and wash the fish with 10mL of ethanol in 2-3 parts. The tail cover and the cleaning solution were transferred into the Erlenmeyer flask together, and then the Erlenmeyer flask was placed on a speed-re...

Embodiment 2

[0072] Repeatability and recovery rate of the inventive method

[0073] According to the reagent preparation and analysis steps of Example 1, the sidestream smoke of a certain cigarette sample was collected and processed, and measured in parallel 6 times according to the method of Example 1, and the repeatability results of the samples were obtained as shown in the following table:

[0074] parallel

1#

2#

3#

4#

5#

6#

average

RSD(%)

Content (ng / cig)

43.86

48.14

42.15

44.68

47.07

45.29

45.20

4.80

[0075] The results in the table show that the relative standard deviation is less than 5%, indicating that the content of ethyl carbamate in cigarette sidestream smoke can be accurately determined.

[0076] The standard sample with the lowest concentration (5.0ng / mL) was injected 10 times, and the standard deviation was calculated from the measured concentration value. Th...

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Abstract

The invention discloses a method for detecting ethyl urethane content of cigarette sidestream smoke via LC-MS / MS combination. A fishtail hood and a glass fiber filter disc are utilized to collect ethyl urethane of cigarette sidestream smoke; ethanol is applied to extraction of the glass fiber filter disc and cleaning of the fishtail hood; and an extraction liquid is diluted and then purified by a solid phase extraction small column, and finally eluted via dichloromethane, an eluant is detected by an efficient liquid chromatogram / tandom mass spectrometer, and the content of the ethyl carbamate of the cigarette side flow smoke. The method is simple and convenient to operate, has a good separation effect and accurate determination, and is short in sample analysis time and high in detection sensitivity.

Description

technical field [0001] The invention belongs to the technical field of tobacco and chemistry, and in particular relates to a method for detecting the content of ethyl carbamate in cigarette sidestream smoke by using high-performance liquid chromatography-tandem mass spectrometry. Background technique [0002] During the combustion process of cigarettes, various physical and chemical reactions such as distillation, dry distillation, pyrolysis, synthesis, and condensation have occurred, forming a large number of nascent compounds, and the composition of the smoke is very complex. Sidestream smoke is the smoke substance released directly from the cigarette burning cone into the air and diffused from the cigarette paper. Most of the sidestream smoke occurs during the smoldering process of the cigarette, and a small part is generated during the smoking process. , Therefore, the types of compounds in mainstream and sidestream smoke are basically the same, but the content is quite ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/08
Inventor 朱怀远庄亚东张媛刘献军张映熊晓敏朱莹沈晓晨
Owner CHINA TOBACCO JIANGSU INDAL
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