In-situ visual determination method for enzyme activity in plants under environmental stress

A determination method and technology for enzyme activity, which are applied in the directions of biochemical equipment and methods, microbial determination/inspection, and measurement devices, can solve the problem of unreported leaf enzyme spectrum, and achieve the effect of great application prospects.

Active Publication Date: 2020-08-28
JINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, studies on leaf zymograms have not been reported so far

Method used

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  • In-situ visual determination method for enzyme activity in plants under environmental stress
  • In-situ visual determination method for enzyme activity in plants under environmental stress
  • In-situ visual determination method for enzyme activity in plants under environmental stress

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Experimental program
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Effect test

Embodiment 1D

[0048] In situ visual determination of glucosidase, xylanase and acid phosphatase in plants under the stress of embodiment 1DBP

[0049] 1. Method

[0050] Using the in situ zymogram visualization method developed by the present invention to characterize the enzymatic activity of glucosidase, xylanase and acid phosphatase in plant leaves under DBP stress, the method flow is as follows figure 1 As shown, it specifically includes the following steps:

[0051] 1. Plant growth and exposure

[0052] Taking the model plant of the research group, Lubao Caixin as the research object, sand culture was carried out in an artificial climate box (constant temperature 25°C, relative humidity 60%), and the improved 8692 nutrient solution was watered every day (see Table 1 for the formula). After 18 days of growth, 3 pretreated seedlings of similar size were selected and placed in centrifuge glass tubes with 50 mL of DBP (0, 10.0, 50.0 and 100.0 mg / L). The concentrations of these DBP solut...

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Abstract

The invention discloses an in-situ visual determination method for enzyme activity in plants under environmental stress. The in-situ visual determination method comprises the following steps: plant pretreatment; enzyme spectrum imaging; standard curve production; and image processing. The invention provides the in-situ visual determination method of enzyme activity in plants under pollutant stress, and makes up the technical blank of plant leaf enzyme spectrum in the prior art. Meanwhile, by providing a series of image processing means, a leaf enzyme spectrum high-quality image is reconstructed, so that useful information of qualitative accuracy is improved, and the method has greater application prospects.

Description

technical field [0001] The invention relates to the technical field of visual phytotoxicity evaluation, in particular to an in-situ visualization technique for characterization of the spatial distribution of enzyme activities related to C and P metabolism in plant leaves under the stress of pollutants, and more specifically relates to the characterization of the spatial distribution of enzyme activities related to plant leaf C and P metabolism under the stress of phthalates. An in situ visualization method for the characterization of the spatial distribution of glucosidase, xylanase, and acid phosphatase activities in medium. Background technique [0002] Phthalates (PAEs) are an important class of synthetic organic compounds, widely used in plastic plasticizers (commonly known as plasticizers), as well as industries such as automobiles, clothing, cosmetics, lubricants and pesticides, and enter the environment in large quantities. Medium, and semi-volatile and long-distance ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/42C12Q1/34G01N21/64G06T7/00G06T7/11G06T7/136G06T7/90G06T5/00
CPCC12Q1/42C12Q1/34G01N21/6402G06T7/0002G06T7/11G06T7/136G06T7/90G06T5/002G01N2333/942G01N2333/916G01N2333/924G06T2207/10064G06T2207/30188
Inventor 冯宇希莫测辉陈昕冯乃宪李彦文蔡全英
Owner JINAN UNIVERSITY
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