Method for detecting urotropin in cigarette mainstream smoke through high performance liquid chromatography-tandem mass spectrum

Abstract

The invention claims a method for detecting urotropin in cigarette mainstream smoke through high performance liquid chromatography-tandem mass spectrum. The method comprises the steps of gathering mainstream smoke total particulate matter of 20 cigarettes according to the rule of ISO4387:2000, then putting a cambridge filter with the mainstream smoke total particulate matter into a 250mL bread bottle, adding 40 mL of ethanol extract liquor containing triethylamine to oscillate for extracting the cambridge filter for 40 minutes, then filtering through a 0.45 micron filter membrane to detect by using GC-MS / MS (Liquid Chromatography-Mass Spectrometry / Mass Spectrometry). Instrument conditions are as follows: a HILIC (Hydrophilic Interaction Chromatography) is used; the detection temperature is 30 DEG C; the sample size is 2microliter; the flow velocity is 0.25mL per minute; a ratio of a mobile phase V (acetonitrile) to V (5mmol / L ammonium acetate solution) is 75:25; the mobile phase carries out isocratic elution. Mass spectrum conditions are as follows: an ionization mode is electros spray; a scanning mode is positive ion; the atomizing gas is 40 psi; the curtain gas is 10 psi; the auxiliary trim gas is 40 psi; the collision gas is 5 psi; the ion source voltage is 4000-5000V; the ion source temperature is 250-350 DEG C. The method is simple to operate, good in separation effect, accurate to determine the nature, short in sample analysis time and high in detection sensitivity.

Description

technical field

[0001] The invention belongs to the technical field of cigarette smoke, and in particular relates to a method for detecting urotropine in cigarette mainstream smoke by high performance liquid chromatography-tandem mass spectrometry (LC-MS / MS). Background technique

[0002] Urotropine is a harmful chemical component, corrosive, and can cause burns to the human body. Its melting point is 263°C. If it exceeds this melting point, it will sublimate and decompose, but it will not melt. When the temperature rises to 300°C, hydrogen cyanide is released, and when the temperature rises again, it decomposes into methane, hydrogen and nitrogen. When the human body contacts the product, it mainly causes dermatitis and eczema. Hexatropine is distributed in the mainstream and side stream smoke of cigarettes. Hexaamine in the smoke is a secondary product of the smoke, which is produced by the reaction of ammonia and formaldehyde in the smoke. The side stream smoke The cont...

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