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Method used for detecting caffeic acid content in cigarette sidestream smoke by high performance liquid chromatography-tandem mass spectrum

A high-performance liquid chromatography, side-stream flue gas technology, applied in the fields of tobacco and chemistry, can solve problems such as low caffeic acid content, matrix interference, and undiscovered, and achieve the effect of improving sensitivity

Active Publication Date: 2013-11-20
CHINA TOBACCO JIANGSU INDAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, during the combustion process of cigarettes, various physical and chemical reactions such as distillation, dry distillation, pyrolysis, synthesis, and condensation have occurred, forming a large number of new compounds, and how to measure whether caffeic acid is distributed in sidestream smoke of cigarettes There are few relevant literature reports. Up to now, no patents or literature reports on the determination of caffeic acid content in cigarette sidestream smoke by high performance liquid chromatography-tandem mass spectrometry have been found at home and abroad.
Moreover, the smoke composition is very complex, the matrix interference is serious, and the content of caffeic acid in the smoke is low. Whether the established detection method for phenolic compounds in the smoke can be applied to the determination of caffeic acid in the smoke is not yet known.

Method used

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  • Method used for detecting caffeic acid content in cigarette sidestream smoke by high performance liquid chromatography-tandem mass spectrum
  • Method used for detecting caffeic acid content in cigarette sidestream smoke by high performance liquid chromatography-tandem mass spectrum
  • Method used for detecting caffeic acid content in cigarette sidestream smoke by high performance liquid chromatography-tandem mass spectrum

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Embodiment 1

[0038] A method for measuring caffeic acid content in cigarette sidestream smoke by high performance liquid chromatography-tandem mass spectrometry, the method comprising the steps of:

[0039] 1. Collection of samples: equilibrate a brand of cigarettes to be tested at (22±1)°C and relative humidity (60±3)% for 48 hours, then determine and mark the length of the cigarette butts for smoking according to YC / T185 Sidestream smoke suction method, adjust the suction flow rate of the sidestream smoking machine to 3000mL / min, smoke 5 cigarettes, and use the fishtail hood and glass fiber filter to capture the total particulate matter in the sidestream smoke.

[0040] 2. Filter extraction and sample determination: put the filter in a 100mL Erlenmeyer flask, add 10mL of methanol solution containing about 120 nanograms (ng) of internal standard, and wash the fish tail with 10mL of methanol for 2 to 3 times Cover and transfer the cleaning solution into the Erlenmeyer flask, and place the ...

Embodiment 2

[0060] A method for measuring caffeic acid content in cigarette sidestream smoke by high performance liquid chromatography-tandem mass spectrometry, the method comprising the steps of:

[0061] 1. Collection of samples: equilibrate a certain brand of cigarettes at (22±1)°C and relative humidity (60±3)% for 48 hours, then determine and mark the length of the cigarette butt for smoking, according to YC / T185 side flow Smoke suction method, adjust the suction flow rate of the side stream smoking machine to 3000mL / min, smoke 5 cigarettes, and use the fishtail hood and glass fiber filter to capture the total particle phase of the side stream smoke.

[0062] 2. Filter extraction and sample determination: put the filter in a 100mL Erlenmeyer flask, add 10mL of methanol solution containing about 120 nanograms (ng) of internal standard, and wash the fish tail with 10mL of methanol for 2 to 3 times Cover and transfer the cleaning solution into the Erlenmeyer flask, and place the Erlenmey...

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Abstract

The invention discloses a method used for detecting caffeic acid content in cigarette sidestream smoke by high performance liquid chromatography-tandem mass spectrum. The method comprises following steps: (1) total particulate matters in smoke are collected by a fishtail cover and a filter disc; (2) the filter disc is placed in a methanol solution containing internal standard substances for extraction so as to obtain an extract; (3) the extract is filtered, and then is detected by a high performance liquid chromatography-tandem mass spectrometer; and (4) the content of caffeic acid is quantitatively analyzed by internal standard method. According to the method used for detecting caffeic acid content in cigarette sidestream smoke by high performance liquid chromatography-tandem mass spectrum, pre-treatment is simple, separating effect is excellent, qualitative analysis is accurate, sample analysis time is short, and detection sensitivity is high.

Description

technical field [0001] The invention relates to the technical fields of tobacco and chemistry, in particular to a high performance liquid chromatography-tandem mass spectrometry method for caffeic acid in sidestream smoke from cigarettes. Background technique [0002] At the molecular level, caffeic acid can cause breakage of the double-strand structure of DNA in vitro experiments. At the cellular level, the compound has been found to cause chromosomal aberrations and sister chromosome exchange. The International Agency for Research on Cancer of the World Health Organization (IARC) classified it as a 2B carcinogen, and the impact of this compound on human health has been widely concerned. In 2001, Hoffmann and his researchers revised and supplemented the list of harmful components in 1991, and listed caffeic acid as one of the 69 harmful components in cigarette smoke. In 2002, Rodgman and Green conducted a review of the reported harmful components in smoke In summary, caffe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/89
Inventor 朱怀远庄亚东张媛尤晓娟秦艳华盛金曹毅刘献军朱莹韩开冬沈晓晨熊晓敏张华
Owner CHINA TOBACCO JIANGSU INDAL
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