Method for detecting four nitrosoamino acids in buccal cigarette by liquid chromatography-tandem mass spectrometry

A technology of tandem mass spectrometry and liquid chromatography, which is applied in the field of liquid chromatography-tandem mass spectrometry to detect four kinds of nitroso amino acids in snus. Positive, safe and efficient effect of pretreatment

Inactive Publication Date: 2020-06-12
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a liquid chromatography-tandem mass spectrometry method for detecting four kinds of nitroso amino acids in snus ( NSAR, NMPA, NMBA, NAzCA) methods

Method used

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  • Method for detecting four nitrosoamino acids in buccal cigarette by liquid chromatography-tandem mass spectrometry
  • Method for detecting four nitrosoamino acids in buccal cigarette by liquid chromatography-tandem mass spectrometry
  • Method for detecting four nitrosoamino acids in buccal cigarette by liquid chromatography-tandem mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Weigh 1g of the gum-based sample into a 50mL extraction centrifuge tube, add 1mL of n-hexane to disperse the sample. Add 10 mL of deionized water containing the internal standard, vortex at 2500 rpm for 30 min, take 5 mL of the extract, add it to an ISOLUTE diatomaceous earth solid-phase supported liquid-liquid extraction column (BIOTAGE isolute SLE), and elute three times with 30 mL of ethyl acetate , 10 mL each time, collect the eluate, concentrate to dryness at 45°C, redissolve with 0.5 mL deionized water, and mix well. Finally, it was analyzed by liquid chromatography-tandem mass spectrometry (LC-MS / MS).

[0033] NSAR and NAZCA were not detected in the gum-based snus, but NMPA and NMBA were contained.

Embodiment 2

[0035]Weigh 1g of American-style wet smoke directly into a 50mL extraction centrifuge tube. Add 10 mL of deionized water containing an internal standard, vortex at 2500 rpm for 30 min, take 5 mL of the extract, add it to an ISOLUTE diatomaceous earth solid-phase supported liquid-liquid extraction column (BIOTAGEisolute SLE), and use 30 mL of ethyl acetate to elute three times. 10 mL each time, collect the eluate, concentrate to dryness at 45°C, redissolve with 0.5 mL deionized water, and mix well. Finally, it was analyzed by liquid chromatography-tandem mass spectrometry (LC-MS / MS). The test results of 4 American wet smoke are shown in Table 6.

[0036] The sample test result of table 6 nitroso amino acids (by dry weight, ng / g)

[0037]

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Abstract

The invention relates to a method for detecting four nitrosoamino acids (including NSAR, NAzCA, NMPA and NMBA) in buccal cigarette by liquid chromatography-tandem mass spectrometry. The method is characterized by comprising the following steps: carrying out vortex extraction on a buccal cigarette sample by adopting deionized water, adding a supernatant into a diatomite solid-phase support liquid-liquid extraction column, performing eluting by using an organic solvent, a collecting eluent, concentrating until the eluent is dry, performing redissolving by using deionized water, and finally performing analyzing by using a liquid chromatography-tandem mass spectrometer (LC-MS/MS). The method has the outstanding advantages that the sample does not need derivatization treatment, the pretreatmentis relatively simple, the accuracy is high, the sensitivity is high, the precision is good, the adding standard recovery rate is high, and the method is suitable for analyzing large-batch samples. Simultaneous detection of four nitrosoamino acids of various buccal cigarettes can be realized.

Description

technical field [0001] The invention belongs to the research of harmful components in snus, and in particular relates to a liquid chromatography-tandem mass spectrometry method for detecting four kinds of nitroso amino acids in snus. Background technique [0002] Snus is a class of smokeless tobacco products that are used through the mouth. The product forms include American moist tobacco (moistsnuff), Swedish tobacco (snus), dissolvable tobacco products (DSP), gum-based Tobacco products (chewing gum), chewing tobacco, dry snuff, etc. The difference between these products and traditional cigarettes is that consumers can directly swallow or chew these products. [0003] In recent years, restrictions and bans such as the World Health Organization Framework Convention on Tobacco Control (WHO FCTC) prohibiting the spread of tobacco smoke in public places have had a strong impact on traditional cigarettes. Under this background, the tobacco industry of various countries in the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/08G01N30/34G01N30/88
CPCG01N30/06G01N30/08G01N30/34G01N30/88G01N2030/062G01N2030/045G01N2030/884
Inventor 王冰王晓瑜余晶晶蔡君兰谢复炜王昇秦亚琼赵晓东刘克建华辰凤
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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