A method for controlling the effect of hydrophilic modification inside a microfluidic chip

Abstract

The invention provides a method for controlling the hydrophilic modification effect inside a micro-fluidic chip, and belongs to the technical field of micro-fluidic chip hydrophilic control. The method is based on the specific and selective recognition effects of aggregation induction luminescent molecules modified by two boric acid groups and PVA containing hydroxyls, the fluorescence visual imaging labeling is performed inside the micro-fluidic chip subjected to hydrophilic treatment; the inside fluorescence intensity and distribution condition can be subjected to measurement and control condition analysis through a fluorescence microscope or a laser co-focusing microscope. The method has the advantages that the in-situ visual control on the micro pore passages in the micro-fluidic chipis realized; the problem that the in-situ detection cannot be realized on the inside of the chip in the conventional measurement can be solved; the method belongs to a safe, nondestructive, fast and effective visual recognition method, and can be widely applied to the materials to realize the fast screening on the hydrophilic treatment effects.

Description

technical field [0001] The invention belongs to the technical field of hydrophilization of microfluidic chips, and in particular relates to a method for specific identification, dyeing and fluorescence imaging control of hydrophilized and modified elastic PDMS chips. Background technique [0002] Microfluidic chips have attracted attention because they can simultaneously realize sample preparation, separation, and detection in a micron-scale environment. Due to its advantages of high accuracy, good sensitivity, and less amount of reactants, it is widely used in the fields of chemistry, biology, and the environment. Among them, polydimethylsiloxane (PDMS) is due to its good optical transparency, simple preparation, and excellent elasticity. And other characteristics have become the main production materials of microfluidic chips. In order to play the important role of PDMS microfluidic chip in the sample reaction system, it is of great significance to hydrophilize its surfac...

AI Technical Summary

Problems solved by technology

Since the pores of the microfluidic chip are at the micron level, the current evaluation of the hydrophilization effect is mostly based on the simulated treatment of the surface of the external material, and it is difficult to directly characterize the inside of the microfluidic chip's pores.

However, the force and structure of the inner and outer surfaces of the channel are different, and the external simulation test cannot represent the true level of the hydrophilic modification effect inside the channel.

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