45results about How to "Short marking time" patented technology

The invention provides an RGD polypeptide coordination complex of a radioactive nuclide label. According to the structure of the coordination complex, the radioactive nuclide is selected from 64Cu, 68Ga, 111In, 62Cu, 67Cu, 67Ga, 86Y, 89Zr or 18F; the ligand is a ligand compound containing an RGD structure, which is shown in a formula (I). The coordination complex has stronger ligand stability and high target/non-target ratio, and the appetency of the ligand with integrin AlphavBeta3 is stronger. The invention further provides a preparation method of the coordination complex, and an application of the coordination complex in preparing tumor developers.

The invention discloses an in-vitro mark for fish and a mark gun of the in-vitro mark. The mark comprises a U-shaped body made of plastic material; a pin is arranged at the inner side of one end of the U-shaped body, and a pin hole corresponding to the pin is arranged at the other end. The mark gun utilizes the mark; the pin on the mark can pass through fin rays on the fish body and is fixed in the pin hole on the U-shaped body to form an annular mark, which is firmly fixed on the base part of each fin ray just as a ring, and an opening of membrane between the fin rays is not increased so as to solve the problem that the mark easily falls off. Only the fin rays are marked; muscle of the fish body is not damaged; the fish death can be reduced, and the survival rate of the discharged fish can be ensured. Meanwhile, the quantity of the marks is large, so that a method for marking by the mark gun is fast; the marking time can be reduced, and the work efficiency can be improved.

The invention discloses a scribing device which comprises a base, a first support frame and a second support frame, wherein the first support frame and the second support frame are fixedly arranged on the upper end face of the base; support tables for supporting scribed workpieces are respectively arranged at the upper ends of the first support frame and the second support frame; a sliding block which can slide on the upper end face of the base is arranged on the upper end face of the base; an upright post is fixedly arranged on the sliding block; a scribing fixed rod is fixedly arranged at the upper end of the upright post; a scribing needle which is fixedly arranged at one end of the scribing fixed rod is arranged at the upper end of the upright post; and a staff gauge is arranged on the base at one side of the sliding block. Workpieces are placed on the support tables at the upper ends of the first support frame and the second support frame, so that one end of each workpiece is aligned to a needle head of the scribing needle; the sliding block is moved within a stroke of a guide rail according to the required scribing length; the staff gauge can be used for accurately measuring the stroke of the scribing block; after accurate positioning, the scribing needle is pressed to scribe on the workpieces and further the workpieces can be accurately scribed. Therefore, the scribing device disclosed by the invention has the advantages of ensured scribing precision, short scribing time and high efficiency.

The invention discloses a preparation method of a gold immunochromatography assay test strip indirectly connecting colloidal gold using a goat-anti-mouse secondary antibody with an anti-mouse marker.The preparation method comprises the following steps: (1) preparing a colloidal gold solution by using trisodium citrate to reduce chloroauric acid; (2) using the colloidal gold solution to mark the goat-anti-mouse secondary antibody; (3) adding the to-be-marked mouse antibody into the colloidal gold solution marking the goat-anti-mouse secondary antibody; (4) processing the indirectly marked mouse antibody colloidal gold solution onto a conjugate pad; (5) processing a corresponding antigen onto a nitric acid cellulose membrane; (6) adhering the nitric acid cellulose membrane, water absorptionpaper, the conjugate pad and a sample pad to a PVC plastic bottom plate; and (7) identifying the quality of the prepared gold immunochromatogrpahy assay test strip according to the product quality standard. The preparation method has the advantages that the detection sensitivity of the gold immunochromatogrpahy assay test strip can be improved, the consumption of antibodies used by the marker canbe reduced, the cost is reduced, the performance of the test paper is improved, and the detection result is stable and reliable.

The invention relates to a <18>O on-line marked protein quantitative analysis platform, and an operation method thereof. The <18>O on-line marked protein quantitative analysis platform is a system integrating protein on-line enzymolysis, <18>O on-line marking and polypeptide separation and detection. The <18>O on-line marked protein quantitative analysis platform comprises an injection pump, a sampling needle, a column oven, an enzyme reactor, a switch valve, a peptide fragment capture column, a liquid phase chromatography pump, a polypeptide separation column and a mass spectrometry detector. A protein sample is firstly loaded to the enzyme reactor through the injection pump to carry out incubation; <18>O marking can be carried out at the same time with enzymolysis; and the <18>O marked peptide fragment after enzymolysis is enriched on-line and transferred into the polypeptide separation column for further separation, and finally is detected by the mass spectrometry detector. The <18>O on-line marked protein quantitative analysis platform integrates the protein on-line enzymolysis, <18>O on-line marking and polypeptide separation.

The invention discloses a scribing device, which comprises a transverse H-shaped pedestal, a sliding rod and a slide block, wherein a supporting plate is arranged in a square hole of the slide block; a scale plate is arranged in a groove; a circular penholder penetrates through a penholder penetration hole; an annular pad on the lower part of the circular penholder is arranged on a Z-shaped limiting block; a spring socket is sleeved on the lower part of the penholder to be arranged between the annular pad and the slide block; and a refill is connected with the circular penholder and is arranged on one plane with paper and a board. The transverse H-shaped pedestal is placed at an initial position where scribing is required, the sharp indicating head is adjusted to point at a corresponding scale, and a set screw is screwed up to fix the slide block on the supporting plate. A clamping head is pulled by one hand to draw one or more parallel lines with equal or unequal spaces on the paper, a wood board and the like. The scribing device has the advantages of simple structure, convenience and quickness in operation, stable and reliable work, capability of simultaneously drawing a plurality of parallel lines with equal or unequal spaces, short scribing time and capability of greatly improving the scribing accuracy and the working efficiency.

The invention provides a cis-platinum complex, and also relates to a synthetic method of the cis-platinum complex and an application of the cis-platinum complex in preparing nucleotide and protein probes. The cis-platinum complex can be connected with fluorescein, and can also be firmly connected with DNA, RNA and proteins, so that the cis-platinum complex can be used as a connection main body of the biological probe and is suitable for marking and detecting the target DNA, RNA and protein; the cis-platinum complex is simple in marking operation procedures, and is not limited to the size of a marking molecule and not limited to a pH value of a marking mixed system; the cis-platinum complex can be widely applied to the subdivisions such as nucleotide, protein (antibody) and protein micro arrays, hybridization of comparative genome and the like; in addition, the marking efficiency is significantly improved, the marking time is shortened, the fluorescent intensity stability is improved, and the marking effect is excellent; and therefore, the application prospect in the fields such as preparation of nucleotide and protein probes, research and development of biological medicine, diagnosis of diseases and the like is good.

The invention relates to an artificial intelligence technology, is applied to a smart city, and provides a method and device for acquiring a KPI abnormal data sample, computer equipment and a storagemedium. The method comprises the following steps: acquiring KPI data in a preset time period; carrying out anomaly detection on the KPI data to obtain a potential abnormal data point, and backtrackingand intercepting specified segment of KPI data as candidate KPI abnormal data according to a time sequence by taking the potential abnormal data point as an end point; adjusting the candidate KPI abnormal data and the known KPI abnormal data to enable the time lengths of the candidate KPI abnormal data and the known KPI abnormal data to be consistent, and performing similarity distance calculation on corresponding data points of the candidate KPI abnormal data and the known KPI abnormal data to obtain a plurality of regular path distances; judging whether the candidate KPI abnormal data are KPI abnormal data or not according to the regular path distances; and if so, marking the candidate KPI abnormal data as KPI abnormal data to serve as a sample for training the intelligent KPI abnormalrecognition model. A large number of KPI abnormal data samples are obtained through a small number of known KPI abnormal data samples, so that the operation and maintenance cost are greatly reduced.

The invention provides a dimeric amido benzimidazole compound. The structure of the dimeric amido benzimidazole compound is shown as a formula (I), wherein R0 is an integer of n from 0 to 5. The invention also provides a compound with anti-tumor activity and a radionuclide labeled compound targeted by interferon stimulating protein, wherein the compound is based on the dimeric amido benzimidazole compound. The dimeric amido benzimidazole compound has high affinity and high specificity to interferon stimulating protein, the compound with anti-tumor activity has the characteristic of activating an STING pathway in vitro, and the radionuclide labeled compound has the advantages that target uptake and non-target uptake are obviously compared, and the lipid solubility can be changed by introducing nuclides with different properties so that the application scene is greatly widened, and the compound can be combined with treatment nuclides for tumor treatment, and even can be used for immunotherapy.

The invention provides novel 18F labeled substituted benzimidazole compounds, which are characterized in that: one end of each compound contains an 18F substituted alkoxy structure, and the other end contains a 6-carboxyl/H benzimidazole structure; a substituent group R1, located on the site 2 of benzimidazole matrix, is hydrogen, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, benzyl, 2-methylthio ethyl and phenyl; a substituent group R2, located on the site 4 of benzimidazole matrix, is hydrogen, methyl and ethyl; and the structure of the compound is shown in formula A, wherein n is between 1 and 5, R2 is H, Me and Et, X is COOH and H. Experiments show that the compounds have high bioactivity such as fast serum removal, high serum stability and relatively low intake in tissues or organs such as liver and relatively high enrichment and slow removal rate in tumor cells, and therefore the compounds have relatively high tumor/background value and are favorable for PET tumor imaging. Meanwhile, the labeled precursor of the compounds is easy to synthesize and the labeling rate is extremely high; and such advantages indicate that the compounds have the tremendous potential to become PET tumor imaging agents.

The invention belongs to the technical field of positron emission computed tomography (PET) imaging, and particularly relates to a method for rapidly marking Cys-Annexin V through <18>F and application of obtained <18>F-Al-NOTA-Cys-Annexin V in the aspect of detecting cell apoptosis. According to the method for rapidly marking Cys-Annexin V through <18>F, <18>F-Al-NOTA-Maleimide is adopted to mark Cys-Annexin V for the first time, the marking time used by the marking method is short, the marking rate is high, the fact of using <18>F for positioning and marking Cys-Annexin V is achieved, and <18>F-Al-NOTA-Cys-Annexin V maintains good affinity with PS, has a good imaging effect by serving as a PET imaging agent and has the more ideal imaging specificity.

The invention relates to the field of machining, and discloses a limited angle torque motor mechanical zero position marking method which is easy to operate, accurate in marking and positioning and capable of greatly reducing marking time, and comprises the steps that S1, arranging a first shaft sleeve, a second shaft sleeve and a pointer at the output shaft end of a limited angle torque motor according to use requirements, arranging an eccentric small ball at the end part of an output shaft of the limited angle torque motor, wherein the eccentric small ball and the output shaft of the limited angle torque motor are integrated, and then carrying out calibration; and S2, starting the limited angle torque motor, firstly controlling the limited angle torque motor to rotate forwards, at the moment, driving a pointer to rotate to a limit angle position at one end by the limited angle torque motor, marking along one side by using a marking pen, then controlling the limited angle torque motor to rotate backwards, and marking along the other side by using the marking pen. And the marked motor finds an angular bisector on the milling machine according to the two lines, and a final marking line is carved, wherein the marking line is the mechanical zero position of the motor.

The invention provides a somatostatin analogue and application thereof. The structure of the somatostatin analogue is as shown in formula I), a Phe-D-Trp-Lys-Thr motif with high affinity to a somatostatin receptor is reserved in the structure of the somatostatin analogue, the probe structure is modified through different chelating agents and different nuclides, and a broad-spectrum radiolabeled somatostatin molecular probe targeting a somatostatin receptor is designed. All subtypes of somatostatin receptors are targeted, and the probe is used as an early diagnosis probe for broad-spectrum neuroendocrine tumors. Therefore, precise medical treatment of early diagnosis, early discovery and early treatment of neuroendocrine tumors is realized.

The invention discloses a novel protein biotin ligase and a proximity labeling system PhastID based on the novel protein biotin ligase. The invention specifically discloses a protein biotin ligase BPL, a modified mutant protein biotin ligase BPL * and an adjacent protein labeling system PhastID based on the biotin ligase BPL or BPL *. Compared with a traditional BioID proximity labeling system, the labeling system containing the protein biotin ligase BPL * has the advantages that the labeling intensity is higher, the required labeling time is shorter, and the application prospect is larger.