Culture method for rice seedlings

Abstract

The invention discloses a culture method for rice seedlings. The rice seedlings are cultured by using a split root culture device; the side faces of two culture bowls of the device are butted; a cover plate and a cultivation plate are arranged above the culture bowls; the cultivation plate is provided with culture holes; the culture method comprises the following steps of: culturing 3-day rice seedlings in kimura nutrient solution with concentration of 50 percent for 2 days; replacing the kimura nutrient solution with the kimura nutrient solution with concentration of 100 percent for 12 days;taking consistently grown seedlings and wrapping stem base parts by using culture cotton; fixing in the culture holes of the cultivation plate; grouping rice seedling roots into equal groups A and B,wherein the group A is placed in the culture bowl containing the kimura nutrient solution, and the group B is placed in the culture bowl containing the culture solution of element deficient; adjusting the pH of the culture solution to 6.0 from 8 a.m. to 15 p.m. each day; and replacing the culture solution after culturing for 3 days, and after culturing to 4-12 days, taking the stems and leaves ofthe rice seedlings, the group A of cultured rice seedling roots and the group B of cultured rice seedling roots. The culture method is easy to operate; the personal error is avoided; the culture timeis saved; and the working efficiency is increased.

Description

technical field [0001] The invention relates to a method for cultivating rice, in particular to a method for cultivating rice by utilizing a root-dividing cultivating device. Background technique [0002] Rice split-root culture technology is widely used in many aspects such as the physiological function of rice nutrients, the movement of nutrients in rice, the effects of nutrients on the growth and development of rice roots, the signal perception and conduction of nutrient deficiency, and the expression of root genes. The technology relates to a rice split root cultivation device and a rice seedling cultivation method. The traditional method of cultivating rice seedlings using a split-root cultivation device is to fix the rice seedlings on a cultivation board, and the nutrient solution culture pot is separated into two nutrition rooms through a middle partition. This method and device are inconvenient for fixing rice seedlings At the same time, when fixing the seedlings, i...

AI Technical Summary

Problems solved by technology

The traditional method of cultivating rice seedlings using a split-root cultivation device is to fix the rice seedlings on a cultivation board, and the nutrient solution culture pot is separated into two nutrition rooms through a middle partition. This method and device are inconvenient for fixing rice seedlings At the same time, when fixing the seedlings, it will affect the fixing of other seedlings. When adjusting the pH value of the solution and changing the nutrient solution, it is easy for one nutrient solution to splash into another nutrient solution, resulting in inaccurate experimental results and inconvenient direct observation. There are many interference factors and large errors in the growth of the root system in the solution and in the cultivation process. The structure of the previous separation and cultivation device is not reasonable, it is inconvenient to operate, increases production costs, reduces work efficiency, and increases labor consumption.

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