Method for detecting single nucleotide polymorphism of sheep PCNP (PEST-Containing Nuclear Protein) gene by using PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) and application of method

Abstract

The invention discloses a method for detecting single nucleotide polymorphism of a sheep PCNP (PEST-Containing Nuclear Protein) gene by using PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) and application of the method. The method comprises the following steps: carrying out PCR amplification on a sheep PCNP gene segment by taking a sheep whole genome DNA to be measured as a template and taking a primer pair P as a primer; after a PCR amplification product is digested by a restriction enzyme SPhI, carrying out agarose gel electrophoresis on the digested amplified segment; authenticating the single nucleotide polymorphism of the 5019th site of the sheep PCNP gene according to an electrophoresis result. As a base mutation site is closely associated with the kidding number of sheep reproduction traits, the method is a method for detecting molecular genetic markers closely associated with the sheep reproduction traits at the DNA level, and can be used for auxiliary selection and molecular breeding of sheep and increasing the improved variety breeding speed of the sheep.

Description

technical field [0001] The invention belongs to the field of molecular genetics, relates to using the single nucleotide polymorphism (SNP) of sheep functional gene as a molecular genetic marker, in particular to the single nucleotide polymorphism of sheep PCNP gene and a detection method thereof. Background technique [0002] In sheep breeding, it is expected that through the selection of DNA markers that are closely related to reproductive traits and closely linked to quantitative traits, the purpose of early selection and improvement of the accuracy of breeding values ​​can be achieved, so as to obtain greater heredity in livestock and poultry breeding. progress. [0003] Molecular breeding, that is, Molecular Mark-Assist Selection (MAS), is a technology that uses DNA molecular markers to select genetic resources or breeding materials, and to improve the comprehensive traits of livestock and poultry. It uses modern molecular The method of combining biology and tradition...

AI Technical Summary

Problems solved by technology

Due to the lack of research on the genetic variation of the PCNP gene in Hu sheep and small-tailed Han sheep in China, the research on the function of the gene locus and the relationship between the genetic variation of the gene and economic traits, such as reproductive traits, have become blank

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