Detecting method and kit of cattle IGF2 (Insulin-like Growth Factor 2) gene mononucleotide polymorphism

A single nucleotide polymorphism and detection method technology, which is applied in the field of detection method and kit for the single nucleotide polymorphism of cattle IGF2 gene, and can solve problems such as changes

Inactive Publication Date: 2013-09-11
NORTHWEST A & F UNIV
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Problems solved by technology

The cSNP in the coding region of the gene can be divided into two types: one is the synonymous cSNP (Synonymous cSNP) in the coding region, that is, the change of the coding sequence caused by the SNP will not affect the change of the amino acid sequence in the protein it translates The other is the non-synonymous cSNP (Non-Synonymous cSNP) in the coding region, that is, the change of the base sequence will lead to the change of the encoded amino acid, which will lead to the change of the amino acid sequence in the protein, which may eventually affect the function of the protein

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  • Detecting method and kit of cattle IGF2 (Insulin-like Growth Factor 2) gene mononucleotide polymorphism
  • Detecting method and kit of cattle IGF2 (Insulin-like Growth Factor 2) gene mononucleotide polymorphism
  • Detecting method and kit of cattle IGF2 (Insulin-like Growth Factor 2) gene mononucleotide polymorphism

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Embodiment Construction

[0032] In the present invention, primers are firstly designed according to the IGF2 gene sequence (GenBank Accession No.EU518675.1, AC_000186.1) published by NCBI, and the genomic DNAs of three cattle varieties are used as templates for PCR amplification, and the PCR products are mixed and purified. , sequenced. Then, the SNP site is screened out by sequencing map analysis and sequence comparison; secondly, the PCR-RFLP detection of polymorphic sites is carried out in the tested population; finally, the population genetic statistical analysis is carried out according to the genotype detected in the population The association analysis with growth traits screened out the molecular markers closely related to the growth traits of cattle. Combine below figure 1 The present invention is described in detail, which is an explanation of the present invention rather than a limitation.

[0033] Cloning of Partial DNA Sequence of IGF2 Gene of I Local Yellow Cattle Breed

[0034] 1. Cat...

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Abstract

The invention discloses a detecting method and a kit of cattle IGF2 (Insulin-like Growth Factor 2) gene mononucleotide polymorphism. The detecting method comprises the following steps of: with IGF2-gene-containing cattle whole genome DNA (deoxyribonucleic acid) to be detected as a template, carrying out PCR (Polymerase Chain Reaction) amplification on the cattle IGF2 gene; and detecting and quickly parting the gene polymorphism through DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology. The detecting method is used for screening and detecting a molecular genetic marker closely related to cattle growth traits on DNA level. The detecting method disclosed by the invention can be used for molecular marker-assisted selection of cattle growth traits so as to quickly create cattle population with excellent genetic resources. The detecting method disclosed by the invention is simple, low in cost, direct and reliable in detecting result and fit for screening and diagnosing the cattle IGF2 gene mutation sites in large scale.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to the detection of gene single nucleotide polymorphism, in particular to a detection method and kit for the single nucleotide polymorphism of cattle IGF2 gene. Background technique [0002] Gene polymorphism refers to the differences in genome sequence between different species or different individuals within the same species. These differences are caused by nucleotide changes in DNA alleles in chromosomes, mainly including base substitutions, insertions, deletions and duplications. Changes in sequence copy number. [0003] According to the location of single nucleotide polymorphism (SNP) in the genome, it can be divided into the following three categories: Coding-region SNPs (cSNPs), single nucleotide polymorphisms around genes Nucleotide polymorphisms (Perigenic SNPs, pSNPs) and intergenic single nucleotide polymorphisms (Intergenic SNPs, iSNPs). Studies have shown that the number of ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 陈宏黄永震李明勋孙雨佳蓝贤勇雷初朝胡沈荣
Owner NORTHWEST A & F UNIV
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