Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Detecting method and kit of cattle IGF2 (Insulin-like Growth Factor 2) gene mononucleotide polymorphism

A single nucleotide polymorphism and detection method technology, which is applied in the field of detection method and kit for the single nucleotide polymorphism of cattle IGF2 gene, and can solve problems such as changes

Inactive Publication Date: 2013-09-11
NORTHWEST A & F UNIV
View PDF0 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cSNP in the coding region of the gene can be divided into two types: one is the synonymous cSNP (Synonymous cSNP) in the coding region, that is, the change of the coding sequence caused by the SNP will not affect the change of the amino acid sequence in the protein it translates The other is the non-synonymous cSNP (Non-Synonymous cSNP) in the coding region, that is, the change of the base sequence will lead to the change of the encoded amino acid, which will lead to the change of the amino acid sequence in the protein, which may eventually affect the function of the protein

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detecting method and kit of cattle IGF2 (Insulin-like Growth Factor 2) gene mononucleotide polymorphism
  • Detecting method and kit of cattle IGF2 (Insulin-like Growth Factor 2) gene mononucleotide polymorphism
  • Detecting method and kit of cattle IGF2 (Insulin-like Growth Factor 2) gene mononucleotide polymorphism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0032] In the present invention, primers are firstly designed according to the IGF2 gene sequence (GenBank Accession No.EU518675.1, AC_000186.1) published by NCBI, and the genomic DNAs of three cattle varieties are used as templates for PCR amplification, and the PCR products are mixed and purified. , sequenced. Then, the SNP site is screened out by sequencing map analysis and sequence comparison; secondly, the PCR-RFLP detection of polymorphic sites is carried out in the tested population; finally, the population genetic statistical analysis is carried out according to the genotype detected in the population The association analysis with growth traits screened out the molecular markers closely related to the growth traits of cattle. Combine below figure 1 The present invention is described in detail, which is an explanation of the present invention rather than a limitation.

[0033] Cloning of Partial DNA Sequence of IGF2 Gene of I Local Yellow Cattle Breed

[0034] 1. Cat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a detecting method and a kit of cattle IGF2 (Insulin-like Growth Factor 2) gene mononucleotide polymorphism. The detecting method comprises the following steps of: with IGF2-gene-containing cattle whole genome DNA (deoxyribonucleic acid) to be detected as a template, carrying out PCR (Polymerase Chain Reaction) amplification on the cattle IGF2 gene; and detecting and quickly parting the gene polymorphism through DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology. The detecting method is used for screening and detecting a molecular genetic marker closely related to cattle growth traits on DNA level. The detecting method disclosed by the invention can be used for molecular marker-assisted selection of cattle growth traits so as to quickly create cattle population with excellent genetic resources. The detecting method disclosed by the invention is simple, low in cost, direct and reliable in detecting result and fit for screening and diagnosing the cattle IGF2 gene mutation sites in large scale.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to the detection of gene single nucleotide polymorphism, in particular to a detection method and kit for the single nucleotide polymorphism of cattle IGF2 gene. Background technique [0002] Gene polymorphism refers to the differences in genome sequence between different species or different individuals within the same species. These differences are caused by nucleotide changes in DNA alleles in chromosomes, mainly including base substitutions, insertions, deletions and duplications. Changes in sequence copy number. [0003] According to the location of single nucleotide polymorphism (SNP) in the genome, it can be divided into the following three categories: Coding-region SNPs (cSNPs), single nucleotide polymorphisms around genes Nucleotide polymorphisms (Perigenic SNPs, pSNPs) and intergenic single nucleotide polymorphisms (Intergenic SNPs, iSNPs). Studies have shown that the number of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 陈宏黄永震李明勋孙雨佳蓝贤勇雷初朝胡沈荣
Owner NORTHWEST A & F UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com