Several Aptamer Sequences Recognizing Benazone and Its Derivatives

A technology of aptamer sequence and derivatives, applied in biological testing, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problem that there is no antibody or aptamer biorecognition material available for bentazone, and achieve The effects of broad environmental detection application prospects
CN106047872BActive Publication Date: 2019-05-28TSINGHUA UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
TSINGHUA UNIV
Publication Date
2019-05-28

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Abstract

The invention provides DNA aptamer sequences for recognizing bentazone and derivatives thereof. DNA aptamer as referred in the invention is single-stranded oligodeoxynucleotide with different oligodeoxynucleotide sequences; and the sequences are capable of recognizing the herbicide bentazone in a solution and applicable as biological recognition materials for detection of the content of bentazone in samples. The DNA aptamer sequences are obtained through in-vitro seletion or SELEX from a random DNA pool. In an aqueous solution, the bonding strength of the DNA aptamer sequences and bentazone molecules dissociation constant enables a dissociation constant (Kd) to reach the magnitude of [mu]M.
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Description

technical field

[0001] The invention belongs to the field of biological recognition materials. The developed nucleic acid aptamer sequence is a biological recognition molecule with superior performance, which can recognize bentazone molecules and derivatives thereof. These nucleic acid aptamer sequences can be used as biological recognition materials to develop reagents, techniques and instruments for detecting bentazone in various samples (including environmental samples, food samples, etc.). technical background

[0002] At present, China's agricultural chemical products have serious environmental pollution problems. For example, after a large amount of herbicides are used disorderly, they enter surface water bodies such as rivers, lakes, and reservoirs through heavy rain runoff, causing serious surface water pollution and threatening aquatic ecosystems. safety and safety of drinking water. Bentazone is a relatively commonly used herbicide [1-2], which has a wide range of...

Claims

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