A method for detecting beef cattle plag1 gene indel marker and its special kit
A gene and marker technology, applied in the field of molecular genetics research, can solve the problem of high cost, achieve high accuracy, avoid specificity and complexity, and have good stability
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[0026] The present invention will be described in detail below with reference to the drawings and embodiments.
[0027] The present invention uses PCR to detect the Indel marker of the PLAG1 gene of Jiaxian Red Cattle and is used for molecular breeding, which usually includes the following steps:
[0028] (1) Use the NCBI database to find the PLAG1 gene sequence, then use the Primer5 software to design the primers, and use PCR to detect the primers;
[0029] (2) Use ordinary PCR technology and agarose gel electrophoresis to detect the typing of candidate sites in the population, and to label different individuals;
[0030] (3) Use SPSS 20.0 software to analyze the correlation between different typing types and cattle growth traits;
[0031] (4) Breeding cattle with excellent growth traits according to different types.
[0032] 1. Cattle sample collection
[0033] The present invention specifically takes 190 Jiaxian red cattle as the detection object, among which blood samples of 190 Jiaxi...
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