A method for detecting beef cattle plag1 gene indel marker and its special kit

A gene and marker technology, applied in the field of molecular genetics research, can solve the problem of high cost, achieve high accuracy, avoid specificity and complexity, and have good stability
CN107988385BInactive Publication Date: 2020-09-29NORTHWEST A & F UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NORTHWEST A & F UNIV
Publication Date
2020-09-29
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for detecting Indel markers of beef cattle PLAG1 genes and a special kit thereof. The method comprises the following steps: based on a PCR (Polymerase Chain Reaction)technology, by taking a blood genome DNA pool of beef cattle (Jiaxian red cattle) as a template, and amplifying Indel sites of cattle PLAG1 genes by utilizing a specific primer pair P1; and performingagarose gel electrophoresis, and dividing different individuals into a deletion form, a normal form and a hybrid form according to electrophoresis results. The method provided by the invention is established on a relevance basis between the Indel sites of beef cattle PLAG1 genes and growth traits, and the method is simple, rapid and convenient to popularize and use and contributes to acceleratingmolecular marker-assisted selection of the beef cattle.
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Description

Technical field

[0001] The invention belongs to the field of molecular genetics research, and specifically relates to a method for detecting the Indel marker of PLAG1 gene of beef cattle (Jiaxian Red Bull). The method uses genomic DNA to perform PCR, then performs agarose gel electrophoresis, and type according to the electrophoresis result. Background technique

[0002] Genetic markers play a pivotal role in the establishment and development of genetics. With the further development of genetics and the emergence of molecular biology, genetic markers have successively undergone morphological markers, cytological markers, biochemical markers and DNA molecular markers. Four stages of development. The first three stages are based on the results of gene expression and are an indirect reflection of genes. The DNA molecular marker is a direct reflection of genetic variation at the DNA level, which can directly reflect the difference at the DNA molecular level. In addition, its polymor...

Claims

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