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A method for detecting beef cattle plag1 gene indel marker and its special kit

A gene and marker technology, applied in the field of molecular genetics research, can solve the problem of high cost, achieve high accuracy, avoid specificity and complexity, and have good stability

Inactive Publication Date: 2020-09-29
NORTHWEST A & F UNIV
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Problems solved by technology

There are also studies using sequencing technology for InDel typing, the error rate is much lower than 1% (Bhangale et al, 2005), but the cost is high

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  • A method for detecting beef cattle plag1 gene indel marker and its special kit
  • A method for detecting beef cattle plag1 gene indel marker and its special kit
  • A method for detecting beef cattle plag1 gene indel marker and its special kit

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Embodiment Construction

[0026] The present invention will be described in detail below with reference to the drawings and embodiments.

[0027] The present invention uses PCR to detect the Indel marker of the PLAG1 gene of Jiaxian Red Cattle and is used for molecular breeding, which usually includes the following steps:

[0028] (1) Use the NCBI database to find the PLAG1 gene sequence, then use the Primer5 software to design the primers, and use PCR to detect the primers;

[0029] (2) Use ordinary PCR technology and agarose gel electrophoresis to detect the typing of candidate sites in the population, and to label different individuals;

[0030] (3) Use SPSS 20.0 software to analyze the correlation between different typing types and cattle growth traits;

[0031] (4) Breeding cattle with excellent growth traits according to different types.

[0032] 1. Cattle sample collection

[0033] The present invention specifically takes 190 Jiaxian red cattle as the detection object, among which blood samples of 190 Jiaxi...

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Abstract

The invention discloses a method for detecting Indel markers of beef cattle PLAG1 genes and a special kit thereof. The method comprises the following steps: based on a PCR (Polymerase Chain Reaction)technology, by taking a blood genome DNA pool of beef cattle (Jiaxian red cattle) as a template, and amplifying Indel sites of cattle PLAG1 genes by utilizing a specific primer pair P1; and performingagarose gel electrophoresis, and dividing different individuals into a deletion form, a normal form and a hybrid form according to electrophoresis results. The method provided by the invention is established on a relevance basis between the Indel sites of beef cattle PLAG1 genes and growth traits, and the method is simple, rapid and convenient to popularize and use and contributes to acceleratingmolecular marker-assisted selection of the beef cattle.

Description

Technical field [0001] The invention belongs to the field of molecular genetics research, and specifically relates to a method for detecting the Indel marker of PLAG1 gene of beef cattle (Jiaxian Red Bull). The method uses genomic DNA to perform PCR, then performs agarose gel electrophoresis, and type according to the electrophoresis result. Background technique [0002] Genetic markers play a pivotal role in the establishment and development of genetics. With the further development of genetics and the emergence of molecular biology, genetic markers have successively undergone morphological markers, cytological markers, biochemical markers and DNA molecular markers. Four stages of development. The first three stages are based on the results of gene expression and are an indirect reflection of genes. The DNA molecular marker is a direct reflection of genetic variation at the DNA level, which can directly reflect the difference at the DNA molecular level. In addition, its polymor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/158
Inventor 黄永震徐微郑立贺花张桂民陈宏雷初朝党瑞华蓝贤勇胡沈荣
Owner NORTHWEST A & F UNIV
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