The invention discloses a blood
metabolism marker for screening heat-stress-resistant dairy cows and application of the blood
metabolism marker. The method comprises the following steps: firstly, under typical
heat stress environmental conditions, collecting indexes such as
rectum temperature,
respiratory rate,
milk yield change and HSP70, Cortisol,
potassium ions and the like in serum of the cows in the
lactation period, and comprehensively scoring the
heat stress response capability of individual cows based on an entropy weight
TOPSIS model to construct a
heat stress resistant group and
heat sensitive group reference group; on the basis, blood samples of two groups of dairy cows are collected, non-targeted
metabolome detection is carried out by adopting a liquid
chromatography-
mass spectrometry technology, differential metabolites remarkably related to the
heat tolerance of the dairy cows are screened out through
multivariate statistical analysis, and 6-ketoprostaglandin F1 alpha is further identified as a candidate
metabolic marker. And a heat stress tolerance identification model is constructed. The method can be used for
molecular level evaluation of
heat resistance of
dairy cattle individuals and heat-resistant cattle screening, has the advantages of high stability, strong specificity, good generalizability and the like, and is suitable for
dairy cattle seed selection, breeding and feeding management in a high-temperature environment.