DNA aptamer sequences for recognizing bentazone and derivatives thereof

A nucleic acid sequence and derivative technology, applied in biological testing, biochemical equipment and methods, material inspection products, etc., can solve the problem that there is no antibody or aptamer biorecognition material available for bentazone, and achieve a wide range of environmental detection The effect of applying the foreground

Active Publication Date: 2016-10-26
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although mature antibodies or nucleic acid aptamers are available for some environmental pollutants, there are currently no

Method used

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  • DNA aptamer sequences for recognizing bentazone and derivatives thereof
  • DNA aptamer sequences for recognizing bentazone and derivatives thereof
  • DNA aptamer sequences for recognizing bentazone and derivatives thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0009] 1. Nucleic acid random library synthesis

[0010] (1) Synthetic steps:

[0011] Take out the solid-phase synthesis column (it can be stored without water, generally stored in a freezer at -20°C, and it can be used after taking it out to restore room temperature); turn on the computer, solid-phase synthesizer, and air pump (turn on the high-purity argon, loosen the partial pressure valve, adjust The valve makes the pressure expression to the specified range);

[0012] Open the software MM;

[0013] Create a text document in advance to edit and synthesize the sequence of the random library, format: name + half-width comma + sequence (the order is from 5' end to 3' end);

[0014] Check the existing method to ensure that the base is the corresponding channel, and check whether the liquid flow out of the pipeline is normal;

[0015] Set up the random library synthesis program and start the reaction;

[0016] After the reaction is over, take out the solid-phase synthesis ...

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Abstract

The invention provides DNA aptamer sequences for recognizing bentazone and derivatives thereof. DNA aptamer as referred in the invention is single-stranded oligodeoxynucleotide with different oligodeoxynucleotide sequences; and the sequences are capable of recognizing the herbicide bentazone in a solution and applicable as biological recognition materials for detection of the content of bentazone in samples. The DNA aptamer sequences are obtained through in-vitro seletion or SELEX from a random DNA pool. In an aqueous solution, the bonding strength of the DNA aptamer sequences and bentazone molecules dissociation constant enables a dissociation constant (Kd) to reach the magnitude of [mu]M.

Description

technical field [0001] The invention belongs to the field of biological recognition materials. The developed nucleic acid aptamer sequence is a biological recognition molecule with superior performance, which can recognize bentazone molecules and derivatives thereof. These nucleic acid aptamer sequences can be used as biological recognition materials to develop reagents, techniques and instruments for detecting bentazone in various samples (including environmental samples, food samples, etc.). technical background [0002] At present, China's agricultural chemical products have serious environmental pollution problems. For example, after a large amount of herbicides are used disorderly, they enter surface water bodies such as rivers, lakes, and reservoirs through heavy rain runoff, causing serious surface water pollution and threatening aquatic ecosystems. safety and safety of drinking water. Bentazone is a relatively commonly used herbicide [1-2], which has a wide range of...

Claims

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Application Information

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IPC IPC(8): C12N15/113G01N33/53
CPCC12N15/115C12N2310/16G01N33/53
Inventor 向宇何苗
Owner TSINGHUA UNIV
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