Novel high-sensitivity LM (listeria monocytogene) detection method based on aptamer modified porous alumina membrane

A technology based on porous alumina and Listeria, applied in the field of ultra-sensitive detectors for Listeria monocytogenes, can solve the problems of difficult antibody preparation, expensive antibodies, and time-consuming

Inactive Publication Date: 2016-10-12
GUANGDONG OCEAN UNIVERSITY
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Problems solved by technology

Production of high-affinity antibodies is expensive and time-consuming
Therefore, it is not easy to prepare antibodies against any target molecule

Method used

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  • Novel high-sensitivity LM (listeria monocytogene) detection method based on aptamer modified porous alumina membrane
  • Novel high-sensitivity LM (listeria monocytogene) detection method based on aptamer modified porous alumina membrane
  • Novel high-sensitivity LM (listeria monocytogene) detection method based on aptamer modified porous alumina membrane

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Embodiment Construction

[0021] In the following, the technical solutions in the embodiments of the present invention will be described in detail with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0022] It should be noted that, in this document, the terms "comprising", "comprising" or any other variation thereof are intended to cover a non-exclusive inclusion such that a process, method, article or apparatus comprising a set of elements includes not only those elements, It also includes other elements not expressly listed, or elements inherent in the process, method, article, or apparatus.

[0023] 1 Materials and methods

[0024] 1.1 Materia...

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PUM

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Abstract

The invention relates to a fast ultra-sensitive LM (listeria monocytogene) detector based on nanochannel confinement characteristics and constructed according to the nature of specific identification between a target molecule and an aptamer of the target molecules. The invention further relates to a method for detecting the LM by taking potassium ferricyanide ions as probe ions and taking an LM DNA modified porous alumina membrane as an electrode for assembling a self-made electrolytic tank. When LM is present and the concentration is lower, a current increasing value is remarkably reduced with the increase of the concentration; a current change value is reduced with increase of the LM concentration; when the LM concentration is in a range of 100-1,250 CFU/mL, a linear relation is formed between the LM concentration and the current increasing value; when the LM concentration is higher than 1,500 CFU/mL, the current change value becomes stable. Therefore, the lowest detection limit of the detection method for the LM can reach 100 CFU/mL, the linear range is 100-1,250 CFU/mL, and the detection can be completed within 10 min; a 108 CFU/mL of escherichia coli and staphylococcus aureus control experiment indicates that the method has high selectivity on the LM.

Description

technical field [0001] The invention relates to an ultrasensitive detector for Listeria monocytogenes based on the confinement property of a nanochannel constructed by using the property of specific recognition between a target molecule and its aptamer. The present invention also relates to the porous aluminum oxide film modified by the DNA aptamer of Listeria monocytogenes as an electrode to assemble a self-made electrolytic cell; the potassium ferricyanide ion is used as a probe ion to rapidly and efficiently treat Listeria monocytogenes. method for sensitive detection. Background technique [0002] Listeria monocytogenes (scientific name: Listeria monocytogenes) is a facultative anaerobic bacterium that is the causative agent of listeriosis; it is a Gram-positive bacterium belonging to the Firmicutes phylum. Listeria monocytogenes (hereinafter referred to as LM) is a zoonotic pathogen. Together with Escherichia coli O157:H7, Staphylococcus aureus and Salmonella, it is l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/327
CPCG01N27/26G01N27/327
Inventor 李承勇周浓周春霞王娟
Owner GUANGDONG OCEAN UNIVERSITY
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