46results about How to "Detection has no effect" patented technology

The invention relates to a Listeria monocytogenes rapid ultra-sensitive detector which is based on nanochannel confinement properties and constructed through utilizing properties of specific recognition between a target molecule and its aptamer and further relates to a self-made electrolytic cell through taking a porous alumina film modified with a Listeria monocytogenes DNA aptamer as an electrode; potassium ferricyanide ions act as probe ions, and Listeria monocytogenes are detected. When the LM is present and the concentration is low, a current rising value decreases significantly with theincreasing concentration; as the concentration of the LM increases, a current change value decreases; when the LM concentration is in the range of 100-1250 CFU / mL, the linear relationship with the current rising value is realized; when the concentration is greater than 1500 CFU / mL, the current change value tends to be stable. Therefore, the detection limit for the LM can reach 100 CFU / mL, the linear range is 100-1250 CFU / mL, and detection can be completed in 10 minutes. A 108 CFU / mL E. coli and S. aureus control experiment showed that high selectivity for Listeria is realized.

The invention discloses a glutathione reductase detection reagent kit which is prepared from the following components: a first reagent and a second reagent. The first reagent is prepared from the components of a phosphate buffer solution, EDTA-NA2, ascorbic acid oxidase, potassium ferricyanide, a preservative and oxidized glutathione. The second reagent is prepared from the components of a trimethylamine methane buffer solution, EGTA, a preservative and reduced coenzyme. The first reagent and the second reagent are both liquid and can be stored for 12 months at 2-8 DEG C environment. Potassiumferricyanide can oxidize hemoglobin to produce stable hemiglobincyanide, and the influence of detection results is reduced. The second reagent adopts EGTA, the linear relationship between the detection results and the change rate of absorbance is can be improved, and adopting of two-point calibration is convenient. The invention further discloses application using the glutathione reductase detection reagent kit. The application comprises the steps that the first reagent and the second reagent are added into a sample solution separately and put into a biochemical analyzer for incubating, the absorbance is read and the change rate of absorbance delta A1 is calculated, and then the glutathione reductase activity of a sample is calculated.

The invention relates to the technical field of fluorescence detection and provides a method for detecting H 2 The fluorescent probe of S and its preparation method and application, the preparation steps are as follows: Step 1, weigh phenanthrenequinone and p-hydroxybenzaldehyde and dissolve them in absolute ethanol, then add ammonium acetate to obtain mixed solution A, and heat and stir the mixed solution reaction; after the reaction is finished, cool to room temperature, filter the precipitate under reduced pressure to obtain a filter cake, and recrystallize with a solvent to obtain an intermediate product; step 2, combine the intermediate product obtained in step 1 with 2,4-dinitrofluorobenzene Add in acetonitrile, and then add anhydrous potassium carbonate to obtain a mixed solution B, stir at room temperature for reaction, remove the solvent after the reaction, and purify by column chromatography to obtain a fluorescent probe for detecting H2S. The invention develops a novel high-performance hydrogen sulfide fluorescent probe, which is successfully applied to detect hydrogen sulfide in food.

The invention discloses a copper ion detection method based on click chemistry and a detection kit. The bivalent copper ion is reduced to the cuprous ion under the effect of a reducing agent, so that the click chemistry reaction is activated; two sections of nucleotide sequences are connected with each other; a catalytic nucleic acid structure with catalytic activity can be further assembled after the magnetic bead separation; a substrate nucleic acid can be cut, so that fluorescence groups and quenching groups at the two ends of the substrate nucleic acid can be separated; and the copper ion concentration can be identified through fluorescence detection. The copper ion detection method has higher sensitivity; a detection limit to copper ions is 2pM; the detection has excellent specificity; the other conventional interfering ions have no influence on the detection; and the detection process is simple in operation, low in cost and suitable for quick detection for the copper ion concentration in practical samples.

The invention discloses a method for online real-time detection of the vehicle battery. The method comprises the following steps: S1, acquiring a storage battery voltage value of a vehicle needing tobe detected in real time; S2, uploading the acquired storage battery voltage value to a platform server; S3, generating the acquired storage battery voltage value into a voltage curve, and performingcloud analysis on the voltage curve to form a voltage curve trace diagram that changes with the storage battery use time; and S4, determining whether the voltage curve of the current storage battery deviates from the track, and predicting that the storage battery needs to be replaced if the deviation value reaches an expected value, and feeding back the information to a terminal device.

The invention discloses a molecular detecting method based on an exonuclease and a G tetramer and a detecting kit. Molecules are interacted with a nucleic acid aptamer, chain replacement reaction is started, a stem-loop structural DNA is opened, and then, an exonuclease mediated signal amplification technology is combined, so that a great number of G tetramer structures with activity can be generated. G tetramers are combined with hemin so as to have the catalytic activity of HRP and be capable of catalytically oxidizing TMB to generate blue substrates, a result can be seen by naked eyes, and the concentrations of molecules are directly related to the depth of blue. The molecular detecting method is simple in operation, capable of finishing the whole reaction at the room temperature, relatively high in sensitivity and favorable in specificity and can be used for rapidly detecting molecules on site; the detection limit for 17b-estradiol is 1 pM; and the detected result can be directly seen with naked eyes, but no detecting instruments are used.

A passive islanding detection method. The method comprises: after grid-connected operation of a power generation system, first determining, in each power grid period, the relationship between period value deviation of a grid connection point voltage and a set value, also determining whether effective total harmonic voltage values in multiple consecutive power grid periods and an average value thereof meet preset conditions, counting corresponding islanding occurrence counting variables in the above-mentioned process, and finally determining whether an islanding state occurs according to a counting result of the islanding occurrence counting variables. The method solves the problem of the impact on the quality of electric energy by using an active disturbance means in conventional islanding detection methods or of a larger dead zone existing despite using a passive means. In conjunction with two parameter characteristics, including a grid connection point voltage period and a harmonic, an islanding state can be rapidly detected without a dead zone, and the quality of electric energy is not affected since a passive means is used.

The invention provides a method for amplifying four genes of Botrytis cinerea and a multiplex PCR (polymerase chain reaction) primer set thereof, relating to the field of molecular biology. On the basis of drug-resistant mutant sites of known Botrytis cinerea p-benzimidazole, boscalid, iprodione, fenhexamid and many other bactericides, a PCR primer set for simultaneously amplifying four gene segments beta-tub, BsOS1, erg27 and SdhB is established. The extracted Botrytis cinerea genome can be directly used as a template and subjected to amplification by using the designed primer set to obtain the enriched four gene segments. The multiplex PCR process is used for the first time to simultaneously amplify the four drug-resistant mutant genes of Botrytis cinerea, thereby greatly reducing the workload for drug-resistant site detection in the later period. By optimizing the PCR reaction system, the four gene segments are amplified in one PCR process.