46results about How to "Detection has no effect" patented technology

The invention discloses a molecule detection method and a detection kit based on DNA self-assembly and G tetramers. Molecules and an aptamer interact to start a DNA self-assembly mediated strand displacement reaction, stem-loop structured DNAs are opened continuously and a closed G tetramer sequence are activated, thus generating a large quantity of G tetramer structures with activity. After being combined with hemin, the G tetramers have HRP catalytic activity and can be used for catalytically oxidizing TMB to generate a blue substrate so that the result can be seen with naked eyes. The molecule concentration is directly related to the depth of blue color. The molecule detection method is simple to operate; the whole reaction can be completed at room temperature without use of a tool enzyme; the sensitivity is relatively high, the detection limit for bisphenol A is 1pg / mL; the reaction has very good specificity; the detection result can be seen with naked eyes; no detection instrument is needed; the molecule detection method and the detection kit can be used for quick field analysis on molecules, especially on bisphenol A.

The invention provides a fluorescent probe for detecting peroxynitrite in a cell. The structural formula of the fluorescent probe is shown in the description, wherein R1 and R2 are selected from hydrogen, linear chain alkyl containing 1 to 5 carbon atoms or naphthenic base containing 3 to 8 carbon atoms respectively and separately; R3 is selected from a cyano group or an ester group. The fluorescent probe has high peroxynitrite selectivity, high cell permeability, extremely-short response time, extremely high detection sensitivity and no toxic and side effects to the cell per se, and is suitable for detecting the concentration change of the peroxynitrite in the cell. The fluorescent probe synthetic method is simple, is high in yield, and is suitable for large-scale production.

The invention relates to a fast ultra-sensitive LM (listeria monocytogene) detector based on nanochannel confinement characteristics and constructed according to the nature of specific identification between a target molecule and an aptamer of the target molecules. The invention further relates to a method for detecting the LM by taking potassium ferricyanide ions as probe ions and taking an LM DNA modified porous alumina membrane as an electrode for assembling a self-made electrolytic tank. When LM is present and the concentration is lower, a current increasing value is remarkably reduced with the increase of the concentration; a current change value is reduced with increase of the LM concentration; when the LM concentration is in a range of 100-1,250 CFU / mL, a linear relation is formed between the LM concentration and the current increasing value; when the LM concentration is higher than 1,500 CFU / mL, the current change value becomes stable. Therefore, the lowest detection limit of the detection method for the LM can reach 100 CFU / mL, the linear range is 100-1,250 CFU / mL, and the detection can be completed within 10 min; a 108 CFU / mL of escherichia coli and staphylococcus aureus control experiment indicates that the method has high selectivity on the LM.

The invention discloses a copper ion detection method based on click chemistry and a detection kit. The bivalent copper ion is reduced to the cuprous ion under the effect of a reducing agent, so that the click chemistry reaction is activated; two sections of nucleotide sequences are connected with each other; a catalytic nucleic acid structure with catalytic activity can be further assembled after the magnetic bead separation; a substrate nucleic acid can be cut, so that fluorescence groups and quenching groups at the two ends of the substrate nucleic acid can be separated; and the copper ion concentration can be identified through fluorescence detection. The copper ion detection method has higher sensitivity; a detection limit to copper ions is 2pM; the detection has excellent specificity; the other conventional interfering ions have no influence on the detection; and the detection process is simple in operation, low in cost and suitable for quick detection for the copper ion concentration in practical samples.

The invention discloses an X-ray circularly polarized ranging method. The method includes: modulating ranging signals by utilizing the circularly polarized modulation technology, and adopting Stokes vectors and a Mueller matrix to perform mathematical modeling on polarized signals in each stage in the ranging process; describing changing of the polarization state in the processes of modulation and demodulation on the basis of a signal model, and further explaining detection of left-handed circularly polarized light and right-handed circularly polarized light and the principle of differential demodulation for noise suppression; restoring ranging codes at a receiving station, and reducing background noise and increasing the signal to noise ratio by taking the measure of regenerating ranging codes. X-rays have characteristics of high energy, low propagation loss and high directivity, thereby being suitable for ranging in free space; the circularly polarized modulation technology has higher anti-interference performance, thereby being suitable for information transmission in the free space under harsh conditions.

The invention relates to a preparation method and application of a test strip for fast time-resolved fluorescent immunochromatography quantitative detection of amantadine. The test strip comprises three parts such as a Fusion 5 membrane, a cellulose nitrate membrane and water absorbent paper. According to the principle of the competition law, a time-resolved fluorescent microsphere is taken as an immune marker for accurate and fast quantitative detection of an antigen to be detected.

The invention discloses a glutathione reductase detection reagent kit which is prepared from the following components: a first reagent and a second reagent. The first reagent is prepared from the components of a phosphate buffer solution, EDTA-NA2, ascorbic acid oxidase, potassium ferricyanide, a preservative and oxidized glutathione. The second reagent is prepared from the components of a trimethylamine methane buffer solution, EGTA, a preservative and reduced coenzyme. The first reagent and the second reagent are both liquid and can be stored for 12 months at 2-8 DEG C environment. Potassiumferricyanide can oxidize hemoglobin to produce stable hemiglobincyanide, and the influence of detection results is reduced. The second reagent adopts EGTA, the linear relationship between the detection results and the change rate of absorbance is can be improved, and adopting of two-point calibration is convenient. The invention further discloses application using the glutathione reductase detection reagent kit. The application comprises the steps that the first reagent and the second reagent are added into a sample solution separately and put into a biochemical analyzer for incubating, the absorbance is read and the change rate of absorbance delta A1 is calculated, and then the glutathione reductase activity of a sample is calculated.

The invention discloses immunomagnetic bead and a method for rapidly detecting Shewanella oneidensis. A preparation method of the immunomagnetic bead is characterized in that the immunomagnetic bead can be obtained by incubating a Shewanella oneidensis specific antibody and activated carboxylated polystyrene immunomagnetic microsphere. The rapid detection method comprises the following steps: placing the immunomagnetic bead in a sample solution, fully combining the antibody of the immunomagnetic bead and the Shewanella oneidensis somatic antigen; removing a sample matrix to obtain an immunomagnetic bead-Shewanella oneidensis immune complex, washing, adding a coloured solution to perform a color development reaction, adding a sulfuric acid solution for stopping the color development reaction, detecting the OD450nm value, if the OD450nm value is more than 0.029, the sample contains Shewanella oneidensis. The method has the advantages that the sensitivity is high, the detection limit is 5.0*103cfu / ml, the operation is convenient and rapid, the method can be used for on-site detection; the specificity is good, and the detection result is nearly free from the influence of other common microbe.

The invention discloses a liquid chromatography method for determining clavulanic acid related substances in an amoxicillin and clavulanic acid potassium pharmaceutical composition. According to the method, octadecylsilane chemically bonded silica is used as a filler, an ammonium acetate aqueous solution is used as a mobile phase A, an ammonium acetate aqueous solution-acetonitrile is used as a mobile phase B, linear gradient elution is carried out, and the pH value of the ammonium acetate aqueous solution is 5.60-5.80. The method provided by the invention can be used for effectively detectingthe clavulanic acid impurities in the amoxicillin and clavulanic acid potassium pharmaceutical composition, is high in separation degree, simple and feasible, low in analysis cost, accurate and reliable in result, and convenient for controlling the product quality in the production and quality control process.

The invention discloses an application of a mon-carbonyl curcumin compound-based visual PH fluorescent probe. The visual PH fluorescent probe has a structure as shown in the formula I. The fluorescent characteristic of a series of mon-carbonyl curcumins is very high in the sensitivity to the PH detection, is very high in the selectivity to hydrogen ions, and is very small in the interference to the other metal ions. The probes have wide prospect as being used as an reagent for the PH detection; the groups represented by R1, R2, R3 and R4 in the formula I are respectively to be one of substituent groups such as OH, H, OCHO3, Br; X is one of CH2, CH2-O-CH2, CH2-S-CH2; and n=0-2.

The invention belongs to the technical field of fluorescence detection, and particularly relates to a ratio type near-infrared fluorescent probe for detecting sulfur dioxide (SO2) derivatives, a preparation method and application thereof to detection of SO2 derivatives in crop leaf tissues, SO2 derivatives in atmosphere or SO2 derivatives in soil. M-diethylaminophenol, sodium nitrite, hydrazine hydrate, a palladium-carbon catalyst, ethyl pyruvate, selenium dioxide, 1-ethyl-2-methylquinoline and the like are adopted as the raw materials to prepare the ratio type near-infrared fluorescent probefor detecting the sulfur dioxide derivative, and the fluorescent probe is used for detecting SO2 derivatives in the atmosphere, SO2 derivatives in the soil or SO2 derivatives in crop leaf tissues. Thefluorescent probe is simple in synthesis method, good in SO2 derivative selectivity and high in sensitivity, and the detection environment is suitable for large-range pH. An attempted probe test paper is a simple and portable tool, and can realize rapid on-site detection of sulfur dioxide derivatives in the atmosphere without depending on large-scale instruments and equipment, so that the attempted probe test paper has innovative significance.

The invention relates to a preparation method and application of a quick test strip for testing chloramphenicol by adopting time-resolved fluorescence lateral flow immunoassay quantitative testing. The test strip is composed of a Fusion5 membrane, a nitrocellulose membrane and water absorption paper, according to the competition law principle, time-resolved fluorescence microspheres are taken as an immune marker, and accurate and quick quantitative testing can be conducted on a to-be-tested antigen.

A non-enzymatic SNP detection method based on DNA self-assembly is disclosed by the invention, and comprises the following steps: designing and separating a capture probe CP, a single chain MP, a probe AP1 and a probe AP2; adding the CP, the MP, a nucleic acid ligase and a sample into a reaction solution, mixing uniformly and completing a reaction; making the double-stranded DNA generated in the reaction solution to unwind into a single-stranded DNA; separating the single-stranded DNA connected with a separation mark, washing to remove other free DNA strands; adding the washed single-stranded DNA with the separation mark, the AP1 and the AP2 into the reaction solution to form a self-assembly double strand after a complete reaction; separating the DNA strand connected with the separation mark, washing to remove other free DNA strands; detecting the amount of double-stranded DNA in the washed DNA strand, and determining the amount of SNP. The method has advantages of being high in sensitivity, good in specificity, simple in operation, short in time, low in sample requirement and the like.

The invention discloses a colloidal gold and fluorescence two-in-one data acquisition device and a colloidal gold and fluorescence two-in-one data acquisition method, relates to the field of optical test instruments, and solves the problem that no equipment for simultaneously reading two types of test paper exists due to negative effects of personal errors and environmental optical interference ofcolloidal gold method test on result judgment. In the invention, the main control board sets a detection environment according to the type of the test card; the method comprises the steps that a light source is set, a data processing mode corresponding to the type of a test card is selected, a main control board analyzes the test card according to the data processing mode corresponding to the type of the test card and outputs a result to a display screen, the main control board is used for inquiring the test card corresponding to a bar code, and the test card is divided into a fluorescence detection card and a colloidal gold detection card. According to the invention, the compatibility of principles of two detection methods is realized, and the optical sensor is not provided with a redundant filter, so that the colloidal gold detection is not influenced at all, and the respective detection of colloidal gold / fluorescence is perfectly realized without mutual influence.

The invention discloses a method for online real-time detection of the vehicle battery. The method comprises the following steps: S1, acquiring a storage battery voltage value of a vehicle needing tobe detected in real time; S2, uploading the acquired storage battery voltage value to a platform server; S3, generating the acquired storage battery voltage value into a voltage curve, and performingcloud analysis on the voltage curve to form a voltage curve trace diagram that changes with the storage battery use time; and S4, determining whether the voltage curve of the current storage battery deviates from the track, and predicting that the storage battery needs to be replaced if the deviation value reaches an expected value, and feeding back the information to a terminal device.

The invention relates to a Listeria monocytogenes rapid ultra-sensitive detector which is based on nanochannel confinement properties and constructed through utilizing properties of specific recognition between a target molecule and its aptamer and further relates to a self-made electrolytic cell through taking a porous alumina film modified with a Listeria monocytogenes DNA aptamer as an electrode; potassium ferricyanide ions act as probe ions, and Listeria monocytogenes are detected. When the LM is present and the concentration is low, a current rising value decreases significantly with theincreasing concentration; as the concentration of the LM increases, a current change value decreases; when the LM concentration is in the range of 100-1250 CFU / mL, the linear relationship with the current rising value is realized; when the concentration is greater than 1500 CFU / mL, the current change value tends to be stable. Therefore, the detection limit for the LM can reach 100 CFU / mL, the linear range is 100-1250 CFU / mL, and detection can be completed in 10 minutes. A 108 CFU / mL E. coli and S. aureus control experiment showed that high selectivity for Listeria is realized.

The invention provides a chemically-modified electrode for caffeine detection. The chemically-modified electrode for caffeine detection is obtained by modifying a polished glassy carbon electrode with poly-gold nanometer particles, L-cysteine, Nafion and reduced graphene oxide in sequence. The chemically-modified electrode serves as a detection electrode, a 0.1M H2SO4 buffering solution containing caffeine with the pH being 2.0 serves as an electrolyte, the differential pulse voltammetry is utilized, scanning is carried out for 8-10 turns under the scanning speed of 90-190 mV and the electrode potential of -0.6-2.1 V, the concentration of CAF and the oxidation peak current of CAF are in a good linear relation, and the detection limit is 1.0*10<-9>M. Thus, the electrode shows very high sensitivity, stability, repeatability, long-term stability and low detection limit on detection of caffeine, no pretreatment is needed when the electrode is used for detecting caffeine in drinks, and the electrode can be effectively used for detecting the content of caffeine in drinks sold on the market.

The invention relates to a passive island detection method. The passive island detection method includes the steps: after a power generation system is connected to the grid to run, determining the magnitude relationship between a period value deviation of a grid-connected point voltage and a set value in each grid period, and determining whether the effective value of the total harmonic voltage and the average value of a plurality of consecutive grid periods satisfy the preset conditions at the same time, counting the corresponding island occurrence count variable in the above process, and finally, determining whether the island state occurs according to the counting result of the island occurrence count variable. The passive island detection method solves the problem that a conventional island detecting method uses the active disturbance mode, thus influencing the power quality, or uses the passive mode but has a large dead zone. The passive island detection method can detect the island state quickly without dead zones by combining the two parameter characteristics of the voltage point and the harmonic of the grid point, and has no influence on the power quality because of the passive mode.

The invention discloses a glutathione reductase detection reagent kit which is prepared from the following components: a first reagent and a second reagent. The first reagent is prepared from the components of a phosphate buffer solution, EDTA-NA2, ascorbic acid oxidase, potassium ferricyanide, a preservative and oxidized glutathione. The second reagent is prepared from the components of a trimethylamine methane buffer solution, EGTA, a preservative and reduced coenzyme. The first reagent and the second reagent are both liquid and can be stored for 12 months at 2-8 DEG C environment. Potassiumferricyanide can oxidize hemoglobin to produce stable hemiglobincyanide, and the influence of detection results is reduced. The second reagent adopts EGTA, the linear relationship between the detection results and the change rate of absorbance is can be improved, and adopting of two-point calibration is convenient. The invention further discloses application using the glutathione reductase detection reagent kit. The application comprises the steps that the first reagent and the second reagent are added into a sample solution separately and put into a biochemical analyzer for incubating, the absorbance is read and the change rate of absorbance delta A1 is calculated, and then the glutathione reductase activity of a sample is calculated.

The invention discloses a copper ion detection method based on click chemistry and a detection kit. The bivalent copper ion is reduced to the cuprous ion under the effect of a reducing agent, so that the click chemistry reaction is activated; two sections of nucleotide sequences are connected with each other; a catalytic nucleic acid structure with catalytic activity can be further assembled after the magnetic bead separation; a substrate nucleic acid can be cut, so that fluorescence groups and quenching groups at the two ends of the substrate nucleic acid can be separated; and the copper ion concentration can be identified through fluorescence detection. The copper ion detection method has higher sensitivity; a detection limit to copper ions is 2pM; the detection has excellent specificity; the other conventional interfering ions have no influence on the detection; and the detection process is simple in operation, low in cost and suitable for quick detection for the copper ion concentration in practical samples.

The invention discloses a molecular detecting method based on an exonuclease and a G tetramer and a detecting kit. Molecules are interacted with a nucleic acid aptamer, chain replacement reaction is started, a stem-loop structural DNA is opened, and then, an exonuclease mediated signal amplification technology is combined, so that a great number of G tetramer structures with activity can be generated. G tetramers are combined with hemin so as to have the catalytic activity of HRP and be capable of catalytically oxidizing TMB to generate blue substrates, a result can be seen by naked eyes, and the concentrations of molecules are directly related to the depth of blue. The molecular detecting method is simple in operation, capable of finishing the whole reaction at the room temperature, relatively high in sensitivity and favorable in specificity and can be used for rapidly detecting molecules on site; the detection limit for 17b-estradiol is 1 pM; and the detected result can be directly seen with naked eyes, but no detecting instruments are used.

A non-enzymatic SNP detection method based on DNA self-assembly is disclosed by the invention, and comprises the following steps: designing and separating a capture probe CP, a single chain MP, a probe AP1 and a probe AP2; adding the CP, the MP, a nucleic acid ligase and a sample into a reaction solution, mixing uniformly and completing a reaction; making the double-stranded DNA generated in the reaction solution to unwind into a single-stranded DNA; separating the single-stranded DNA connected with a separation mark, washing to remove other free DNA strands; adding the washed single-stranded DNA with the separation mark, the AP1 and the AP2 into the reaction solution to form a self-assembly double strand after a complete reaction; separating the DNA strand connected with the separation mark, washing to remove other free DNA strands; detecting the amount of double-stranded DNA in the washed DNA strand, and determining the amount of SNP. The method has advantages of being high in sensitivity, good in specificity, simple in operation, short in time, low in sample requirement and the like.

A passive islanding detection method. The method comprises: after grid-connected operation of a power generation system, first determining, in each power grid period, the relationship between period value deviation of a grid connection point voltage and a set value, also determining whether effective total harmonic voltage values in multiple consecutive power grid periods and an average value thereof meet preset conditions, counting corresponding islanding occurrence counting variables in the above-mentioned process, and finally determining whether an islanding state occurs according to a counting result of the islanding occurrence counting variables. The method solves the problem of the impact on the quality of electric energy by using an active disturbance means in conventional islanding detection methods or of a larger dead zone existing despite using a passive means. In conjunction with two parameter characteristics, including a grid connection point voltage period and a harmonic, an islanding state can be rapidly detected without a dead zone, and the quality of electric energy is not affected since a passive means is used.

A method for detecting quality of Likejun preparation includes preparing chromatogram conditions by using silica gel as loading agent and using water - acetonitrile - glacial acetic acid as flowing phase as well as using wavelength of 210 nm as detection wavelength, preparing test solution by putting proper amount of Likejun preparation into acetonitrile of 100 micron g / ml, preparing control solution by putting proper amount of likejun control matter into acetonitrile of 100 micron g / ml, carrying out determination as injecting 10 - 20 micron l of each prepared solution into liquid phase chromatograph, calculating and determining Likejun content in sample.

The invention provides a trace element detection device and method based on a labeling technology, the trace element detection device based on the labeling technology comprises an analysis unit and a first pipeline, and a to-be-detected object passes through the first pipeline and enters the analysis unit; a marker applying unit is used for adding a marker into an object to be detected in the first pipeline; a speed sensor is used for detecting the speed of the marker in the first pipeline so as to obtain the sample injection speed of the to-be-detected object; one end of a second pipeline is communicated with the first pipeline between the analysis unit and the speed sensor, and the other end of the second pipeline is communicated with the conveying unit; and a conveying unit is used for pushing the standard liquid at different standard adding speeds, and the standard liquid enters the first pipeline from the second pipeline. The device has the advantages of high detection precision, automation and the like.

The invention provides a method for amplifying four genes of Botrytis cinerea and a multiplex PCR (polymerase chain reaction) primer set thereof, relating to the field of molecular biology. On the basis of drug-resistant mutant sites of known Botrytis cinerea p-benzimidazole, boscalid, iprodione, fenhexamid and many other bactericides, a PCR primer set for simultaneously amplifying four gene segments beta-tub, BsOS1, erg27 and SdhB is established. The extracted Botrytis cinerea genome can be directly used as a template and subjected to amplification by using the designed primer set to obtain the enriched four gene segments. The multiplex PCR process is used for the first time to simultaneously amplify the four drug-resistant mutant genes of Botrytis cinerea, thereby greatly reducing the workload for drug-resistant site detection in the later period. By optimizing the PCR reaction system, the four gene segments are amplified in one PCR process.

The invention provides a method for detecting serum protein by homogeneous fluorescence detection on the basis of fluorescence resonance energy transfer. The method adopts nanobodies marked by fluorescent dye as energy donors, and adopts homogeneous nanobodies or paired nanobodies marked by a corresponding fluorescent quenching agent as energy receptors. The method provided by the invention has the beneficial effects that with adoption of the nanobodies, the stability is good, and the detection for samples in serum is basically not influenced; the nanobodies marked by the fluorescent dye are used as the energy donors, the homogeneous nanobodies or paired nanobodies marked by the corresponding fluorescent quenching agent are used as the energy receptors, and by adoption of a method of double-antibody sandwiching, the defects of low sensitivity and influence of oxidizing and reducing substances of a serum sample in the immunoassay method are effectively overcome, and the fastness and thesensitivity of the method are ensured.

The invention relates to the technical field of fluorescence detection, in particular to a preparation method and application of a ratio-type near-infrared fluorescent probe for detecting cyanide in food; the preparation steps are as follows: firstly prepare and obtain 7-(diethylamino)coumarin and 7‑(diethylamino)coumarin aldehyde; then 7‑(diethylamino)coumarin aldehyde and (1,3‑dioxychloro‑2‑methyl)triphenylphosphine bromide were added to Dissolve in dichloromethane, add dropwise sodium hydroxide solution, add hydrochloric acid to neutralize, obtain the intermediate product through extraction and separation, and column chromatography purification; add the intermediate product and acetophenone to the mixed solvent of dichloromethane and methanol, and then Add tetrahydropyrrole dropwise, add sodium chloride, stir, distill, and column chromatography purify to obtain the ratio-type near-infrared fluorescent probe for detecting cyanide; the present invention has developed a novel high-performance ratio-type near-infrared fluorescent probe , the synthesis method is simple, has good selectivity to cyanide, and has been successfully applied to the detection of cyanide in food.