A click chemistry-based copper ion detection method and detection kit

A copper ion detection and click chemistry technology, applied in the field of environmental analytical chemistry, can solve the problems of expensive instruments and interference detection sensitivity, and achieve the effects of low cost, high sensitivity and good selectivity
CN107064515BActive Publication Date: 2019-10-18GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
Publication Date
2019-10-18

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Abstract

The invention discloses a copper ion detection method based on click chemistry and a detection kit. The bivalent copper ion is reduced to the cuprous ion under the effect of a reducing agent, so that the click chemistry reaction is activated; two sections of nucleotide sequences are connected with each other; a catalytic nucleic acid structure with catalytic activity can be further assembled after the magnetic bead separation; a substrate nucleic acid can be cut, so that fluorescence groups and quenching groups at the two ends of the substrate nucleic acid can be separated; and the copper ion concentration can be identified through fluorescence detection. The copper ion detection method has higher sensitivity; a detection limit to copper ions is 2pM; the detection has excellent specificity; the other conventional interfering ions have no influence on the detection; and the detection process is simple in operation, low in cost and suitable for quick detection for the copper ion concentration in practical samples.
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Description

technical field

[0001] The invention belongs to the field of environmental analytical chemistry, and in particular relates to a click chemistry-based copper ion detection method and a detection kit. Background technique

[0002] Copper ions (Cu 2+ ) is one of the essential nutrients for the human body, but excessive intake will cause symptoms of poisoning, Cu 2+ Enrichment in the human body will cause serious damage to the brain, liver, pancreas, and heart muscle. The copper ingested by the human body is mainly obtained from water, food and the environment. Therefore, for Cu 2+ The analysis and determination of content is of great significance.

[0003] Common methods for the determination of trace copper mainly include graphene atomic absorption spectrometry and inductively coupled plasma mass spectrometry. However, the instruments used in these methods are expensive and require professional operators, which limits their use in primary laboratories. Cu-based 2+ The id...

Claims

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