A fresh cut flower antistaling agent and a preparing method thereof are provided. The antistaling agent comprises antistaling components and promoting components. The antistaling components comprise ethyl pyruvate, citric acid, sodium lactate, chitosan, acetic acid, sorbic acid, sucrose ester and water. The promoting components comprise polyethylene glycol, sodium bicarbonate, potassium dihydrogen phosphate, ethylene carbonate, alpha-aminoisobutyric acid and absolute ethanol. The preparing method includes separately preparing the antistaling components and the promoting components, adding the promoting components into the antistaling components, and fully mixing the materials by stirring to obtain the antistaling agent. The antistaling agent is simple in preparing method, easily available in raw materials, low in cost, nontoxic, and capable of effectively maintaining the bright-color period of the fresh cut flower and has good preservation functions especially for cut flowers requiring a long transportation period.