7 results about "Somatic antigen" patented technology

The application discloses a mechanism-driven nanobody-antigen binding prediction method and system, relates to the technical field of bio-information processing and artificial intelligence, and inputs amino acid sequences of nanobodies and antigens into a prediction system for processing, and a construction process of the prediction system comprises the following steps: after an encoder encodes the amino acid sequences of the nanobodies and the amino acid sequences of the antigens, global features and local features of respective complementarity determining regions are extracted and fused to obtain nanobody fusion features and antigen fusion features, and the nanobody fusion features and the antigen fusion features are subjected to global average pooling to obtain an encoder feature of the nanobodies and an encoder feature of the antigens; a combination prediction model models the interaction between the encoder feature of the nanobodies and the encoder feature of the antigens through Hadamard product modeling, generates an interaction feature, and predicts a combination probability based on the interaction feature. Through the cooperative work of the mechanism-driven encoder and the prediction system, high-precision and high-efficiency nanobody-antigen binding prediction is realized.

The present disclosure relates to a fibrin (ogen) degradation product detection kit and its preparation and application. The detection method of the kit is magnetic particle chemiluminescence immunoassay, and the reagent composition comprises a first reagent, a second reagent, a third reagent and its calibrator, and a quality control product. The third reagent is a magnetic particle coated with an antibody, which can bind to the analyte in the sample; the first reagent is a sample diluent; the second reagent is an enzyme-labeled antibody reagent, which can bind to the analyte in the sample to form an antibody-antigen-antibody sandwich complex. The kit of the present disclosure realizes high-speed and high-sensitivity detection, and is mainly used for the auxiliary diagnosis of primary and secondary fibrinolysis hyperfunction related diseases in clinical practice.

An immunomodulatory nanoparticle vaccine carrier protein and its application. This invention uses duck hepatitis B virus core antigen (DHBc) as a base, fuses and expresses the SpyCatcher003 sequence to construct DHBc-SpyCatcher (DHBc-SC) virus-like particles with self-assembly capability, and efficiently expresses them in *E. coli*. When DHBc-SC virus-like particles are mixed with heterologous monomeric antigens containing SpyTag, they can spontaneously couple to form virus-like particles displaying the heterologous antigen, significantly enhancing the immunogenicity of the heterologous antigen.

The present invention provides an antibody and an antigen-binding fragment (e.g., a human antibody) that specifically bind to human IL2 receptor gamma (IL2Rγ). A method for treating or preventing a disease mediated by IL2Rγ (e.g., graft-versus-host disease) using the antibody and the fragment is also provided along with a method for preparing the antibody and the fragment.

The application belongs to the technical field of antigen protein and antibody preparation and discloses a Linopneus Genomic Differentiation Factor-8 / 11 polyclonal antibody, antigen protein and a preparation method thereof. The application obtains recombinant expression of the Linopneus Genomic Differentiation Factor-8 / 11 antigen protein, obtains the Linopneus Genomic Differentiation Factor-8 / 11 polyclonal antibody after immunizing mice, and provides an advantageous tool for the research on the Linopneus Genomic Differentiation Factor-8 / 11 protein level. The application also successfully obtains a Linopneus Genomic Differentiation Factor-8 / 11 antigen protein recombinant expression vector, and can obtain the Linopneus Genomic Differentiation Factor-8 / 11 antigen protein. The application also provides a Linopneus Genomic Differentiation Factor-8 / 11 gene coding region full sequence, fills the blank of related research, and the primer pair provided by the application can amplify the above Linopneus Genomic Differentiation Factor-8 / 11 gene coding region full sequence through PCR, and can be used for other related research.

This invention belongs to the field of biomedical technology, specifically relating to a kit for detecting brucellosis and its application. The kit includes an enzyme-linked immunosorbent assay (ELISA) plate coated with a capture antibody, HRP-labeled antibody, antigen standards, washing buffer, chromogenic solution, and stop solution. The heavy chain amino acid sequence of the anti-CTSC protein monoclonal antibody CTSC-1 in the capture antibody is shown in SEQ ID NO.1, and the light chain amino acid sequence is shown in SEQ ID NO.2. The heavy chain amino acid sequence of the anti-CTSC protein monoclonal antibody CTSC-2 in the HRP-labeled antibody is shown in SEQ ID NO.3, and the light chain amino acid sequence is shown in SEQ ID NO.4. The kit for detecting brucellosis provided by this invention has high specificity, can specifically detect the content of CTSC protein in urine, has high sensitivity, and has good application prospects.

本发明公开了一种同时利用多抗体的Multi‑ChIP‑seq检测方法，属于表观遗传学技术领域。本发明将待测样本预处理获得含细胞核反应液，超声获得片段化染色质；抗体池每孔中放置抗体和磁珠，混合均匀得磁珠‑抗体复合物；磁棒架和磁棒套取出磁珠‑抗体复合物，放置在片段化染色质中进行免疫捕获，取出磁珠‑抗体‑抗原复合物放置在每孔抗体中混合均匀，获得磁珠‑抗体‑抗原复合物；将磁珠‑抗体‑抗原复合物洗涤，测序接头连接，解交联去除磁珠，得含有目的DNA溶液；洗脱DNA后加入index引物扩增，测序。该方法可高通量和高精准的实现多靶标检测。