35 results about "Multivalent binding" patented technology

Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

The present invention relates to binding proteins which bind alanine aminotransferase (ALT). The present invention also relates to bivalent, trivalent, quadrivalent or multivalent binding proteins that bind ALT. The present invention also relates to nucleic acids, vectors and host cells for producing such binding proteins, methods for producing such binding proteins, kits comprising such binding proteins and methods and uses of such binding proteins.

Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

Provided herein are multivalent proteins comprising a first region capable of binding to one or more effector proteins and a second region capable of binding to one or more target proteins. Also provided are expression cassettes and vectors comprising nucleic acids encoding the multivalent proteins. Also provided are recombinant cells comprising the expression cassettes and vectors. Also provided are uses of such multivalent proteins, nucleic acids and recombinant cells. Also provided are the multivalent proteins, nucleic acids and recombinant cells for use as medicaments.

The application provides a method and a kit for detecting Staphylococcus aureus. The method embeds multivalent binding probes on microspheres through strain-promoted azide-alkyne cycloaddition reaction, thereby forming microspheres loaded with multivalent binding probes. The microspheres loaded with multivalent binding probes are aggregated in a large size through multivalent binding between the probes and the genome of Staphylococcus aureus, so as to qualitatively and quantitatively identify Staphylococcus aureus through fluorescence. The method not only shortens the detection period and reduces the cost, but also has the advantages of high sensitivity and high specificity.

Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

Described herein are methods to affect binding by a multivalent binding molecule to a FZD receptor and a Wnt co-receptor on a cell wherein binding by the multivalent binding molecule to both FZD receptor and co-receptor on the cell activates a Wnt signaling pathway. Also described herein are multivalent binding molecules comprising a FZD receptor binding domain and a Wnt co-receptor biding domain on either end of an Fc domain that activate a Wnt signaling pathway and methods for their use.

Method for analyzing a sample Su, wherein sample Su comprises an unknown concentration CM_u of a ligand-specific monovalent binding species M and further comprises a ligand-specific multivalent binding species B, the method comprising the steps of an activated sensor surface A1L1 providing step actM comprising providing a sensor surface A1 having a ligand L1 immobilized thereon, wherein the ligand L1 can be monovalently bound by the ligand-specific monovalent binding species M and the ligand L1 can be multivalently bound by the ligand-specific multivalent binding species B, a calibration step calM yielding a monovalent calibration function FA1L1_M, recording in step mesM for the sample Su comprising the unknown concentration CM_u of the ligand-specific monovalent binding species M a sensorgram SGu using the activated sensor surface A1L1, selecting in step selM a response value rA1L1_M_u or the slope of the response value sA1L1_M_u from the sensorgram Su at a selection time tA1L1_M_s, retrieving in step retrM the concentration CM_u of the ligand-specific monovalent binding species M in the sample Su by calculating the concentration form the response value rA1L1_M_u or the slope of the response value sA1L1_M_u using the monovalent calibration function FA1L1_M.

Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

The invention belongs to the field of biological medicine, and particularly relates to a dendritic multi-targeting antibody coupling carrier, an antibody coupling medicine as well as a preparation method and application of the dendritic multi-targeting antibody coupling carrier and the antibody coupling medicine. The dendritic multi-targeting antibody coupling carrier is composed of a targeting module and a drug coupling module, wherein the targeting module has multispecificity and a multivalent binding receptor function; the drug coupling module can realize fixed-point coupling and high drug loading capacity of drugs. The fusion proteins of the two modules can be specifically bound by a spy reaction through spontaneously formed covalent bonds. According to the present invention, the dendritic multi-targeting antibody coupling carrier is further utilized to construct the antibody coupling drug, such that the targeting property of the drug is improved, the relative drug loading capacity of the drug is improved, and the tumor treatment effect of the antibody coupling drug is finally improved.

Compounds of the disclosure include modified dideoxynucleotide triphosphates (ddNPT) including multiple binding unit containing arms to provide multivalent binding unit molecules.

The present invention pertains to the field of biotechnology and bioengineering, specifically to the design and selection of multivalent binders for interrogating protein-protein interactions (PPIs). This invention focuses on novel methods for generating multivalent binders with user-defined spatial organization, using a combination of nucleic acid polymers and a target-tailored multimerization scaffold.

The present application belongs to the field of bionic technology, and particularly relates to a kind of bionic material loaded with YD monomer and its preparation method and application.The present application utilizes YD monomer composed of tyrosine (Y) and aspartic acid (D) to synthesize a kind of bionic material through reversible addition-fragmentation chain transfer (RAFT) polymerization. The bionic material can produce spatially adjacent binding pockets through the covalent bond of tyrosine (Y) and aspartic acid (D), and can capture two kinds of PTM (phosphopeptide and O-glycopeptide) simultaneously through synergistic interaction. The results of the present application show that the bionic material of YD monomer optimizes charge balance, the neutral phenolic group of Y (pKa ~ 10) and the negatively charged carboxylate of D (pKa ~ 3.9) together reduce the electrostatic repulsion with phosphopeptide, while maintaining target affinity; self-assembled into nanostructured porous framework can expose multivalent binding sites, and significantly improve capacity and selectivity through synergistic effect.

The invention belongs to the technical field of immunochromatography detection, and particularly relates to a time-resolved fluorescence immunochromatography test strip for acute stress syndrome detection as well as a preparation method and application of the time-resolved fluorescence immunochromatography test strip. An IgA antibody is introduced into a detection system to serve as a quality control or recognition element, and the detection specificity and stability are further enhanced. By utilizing the IgA antibody, not only can the compatibility of the reagent and the sample be improved, but also the signal strength can be enhanced through the multivalent binding characteristic of the IgA antibody, so that the detection sensitivity is remarkably improved. Therefore, the subtle fluctuation of cortisol and other hormones in the acute stress state can be more clearly captured, the physiological change of the stress reaction is more obviously reflected, and a more accurate, convenient and reliable tool is provided for early screening, auxiliary diagnosis and curative effect evaluation of mental and psychological diseases.

Provided herein are methods of making and / or identifying multivalent antibody conjugates, e.g., multispecific antibody conjugates, such as bispecific antibody drug conjugates, and conjugated multivalent molecules identified by the methods. In particular, the present invention relates to a method of preparing a multivalent binding polypeptide conjugated to a molecule of interest wherein the method comprises combining a first polypeptide conjugated to the molecule of interest with at least one other polypeptide to form a multivalent binding polypeptide conjugated to the molecule of interest. Intermediates, polypeptide constructs, kits and uses are also provided.

This invention discloses a recombinant protein pPIC9K-17, its preparation method, and its applications, relating to the field of gene recombination technology. The recombinant protein pPIC9K-17 provided by this invention does not strictly follow the (Gly-X-Y)n triple helix repeat pattern of traditional collagen in its core structural unit. The repeating unit sequence includes a "GAIQ" sequence and a sequence containing collagen characteristics, possessing a certain rigid structural basis and containing receptor-binding or signal activation domains for specific growth factors. The direct tandem connection of multiple such units theoretically enables multivalent binding, enhancing affinity and signal activation efficiency with cell surface receptors, thus surpassing the biological functional limitations of single components (pure collagen or pure growth factors) or simple repeating structures. The recombinant protein of this invention not only effectively promotes the proliferation and migration of skin fibroblasts and accelerates collagen synthesis, but also shows clear efficacy in promoting hair regeneration (hair growth), and can be used to prepare products for skin repair and / or promoting hair regeneration.

The present application relates to a kind of multispecific and / or multivalent binding protein for preventing and / or treating HIV infection, it includes at least 3 antigen binding sites, part of antigen binding site is specifically bound HIV-1-gp41 or HIV-1-gp120 or HIV-1-gp160, still part of antigen binding site is specifically bound human CD4 receptor or human CCR5 receptor;The binding protein of the present application has excellent broad-spectrum neutralizing ability in the treatment or prevention of HIV infection, provides a new solution for the problem of drug resistance escape caused by HIV virus variation, in addition, the present application inventor also unexpectedly found that the binding protein of the present application can treat or prevent the tumor-related disease caused by HIV infection.

The present disclosure relates to methods for preparing and using multiprotein complexes. The complexes can establish multivalent binding mechanisms for multiple targets in solution or on a surface. The complexes have various applications and can be used, for example, for activation and / or expansion of T cells.

Provided herein are, inter alia, polypeptides and covalent complexes including the same, with multivalent binding specificity. The polypeptides include an Fc receptor portion / domain (e.g., a CD16 domain, a CD32 domain, a CD64 domain and variants thereof) with a cysteine amino acid substitution capapble of forming a disulfide linkeage with a second cysteine amino acid substitution in an Fc domain (e.g., an Fc domain of IgG1, IgG2, IgG3, IgG4) thereby forming a covalent complex with multivalent binding ability. The compositions provided herein exhibit highly specific binding with reduced or no endogenous Fc receptor binding activity thereby resulting in therapeutics that lack undesirable side effects.

This invention provides a method for screening multivalent binding probes for bacterial detection, comprising the following steps: (1) using an algorithm to screen nucleic acid sequences within the top 1000 range of multiplicity scores for the target bacterial genome; (2) performing secondary screening on the nucleic acid sequences obtained in step (1) to obtain multivalent binding probes for bacterial detection, wherein the secondary screening includes at least one of the following: (2-1) screening nucleic acid sequences with G and C base content in the range of 40%-60%; (2-2) screening nucleic acid sequences in which the maximum continuous length of a single G / C base does not exceed 3 and the maximum continuous length of G-C alternation does not exceed 5; (2-3) screening nucleic acid sequences in which the Gibbs free energy change for forming a hairpin structure and the Gibbs free energy change for forming a homodimer structure are both > -5 kcal / mol. This method is scientific and systematic, and can assist technicians in efficiently and accurately judging and selecting multivalent binding probes suitable for bacterial detection.

The invention discloses a nucleic acid detection sensor for multivalent capture by using an S9.6 antibody, and belongs to the technical field of biological detection. The sensor comprises hairpin probes HP1 and HP2, a closed probe, an RNA (Ribonucleic Acid) single-chain probe and an electrode or a chip which is self-assembled with an S9.6 antibody. When target nucleic acid exists, the hairpin probe HP1 and the hairpin probe HP2 are subjected to a hybridization chain reaction to form an HCR compound, the RNA single-stranded probe and an HP2 extension chain in the HCR compound are subjected to a hybridization reaction to form a DNA / RNA heteroduplex chain, the compound is captured on a sensing interface through multivalent binding of the DNA / RNA heteroduplex chain at multiple sites and an S9.6 antibody, a detection signal is generated through catalysis of signal molecules, and then the nucleic acid concentration is judged. And the closed probe is used for eliminating the influence of heteroduplex competitive recognition of the S9.6 antibody in the HP2 / RNA. The sensor provided by the invention can obtain ultrahigh interfacial capture affinity and is less interfered by complex biomolecules.

Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

The present disclosure provides compositions and methods related to antiviral therapeutics. In particular, the present disclosure provides a highly modular platform useful for the development and production of multivalent biomolecules capable targeting and neutralizing a virus.

The present invention pertains to the field of biotechnology and bioengineering, specifically to the design and selection of multivalent binders for interrogating protein-protein interactions (PPIs). This invention focuses on novel methods for generating multivalent binders with user-defined spatial organization, using a combination of nucleic acid polymers and a target-tailored multimerization scaffold.

The present invention relates to multivalent binding molecules that activate WNT signaling and uses thereof. Described herein are methods of affecting the binding of a multivalent binding molecule to FZD receptors and Wnt co-receptors on a cell, wherein binding of the multivalent binding molecule to both FZD receptors and co-receptors on the cell activates the Wnt signaling pathway. Also described herein are multivalent binding molecules that activate the Wnt signaling pathway comprising a FZD receptor binding domain and a Wnt co-receptor binding domain on either end of an Fc domain and methods of using the same.

Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

High-affinity antibodies recognizing CD3 and B Cell Maturation Factor protein (BCMA) are disclosed. Binding sites from humanized anti-CD3 and anti-BCMA antibodies are incorporated into a Fabs-in-Tandem Immunoglobulin format without significant loss of binding affinity, and the resultant bispecific, multivalent binding proteins are able to bind to both CD3 and BCMA simultaneously. Such antibodies, antigen-binding portions thereof, and bispecific FIT-Ig binding proteins are useful for treating cancer.

The present invention relates to a modular multivalent antigen-binding protein complex, use of the antigen-binding protein complex in medicine and use of the antigen-binding protein complex in the prophylaxis, treatment or diagnosis of a disorder or disease.

The compounds of the present disclosure include a modified dideoxynucleotide triphosphate (ddNPT) comprising a plurality of binding units comprising arms to provide a multivalent binding unit molecule.