21 results about "Multivalent binding" patented technology

Provided herein are multivalent proteins comprising a first region capable of binding to one or more effector proteins and a second region capable of binding to one or more target proteins. Also provided are expression cassettes and vectors comprising nucleic acids encoding the multivalent proteins. Also provided are recombinant cells comprising the expression cassettes and vectors. Also provided are uses of such multivalent proteins, nucleic acids and recombinant cells. Also provided are the multivalent proteins, nucleic acids and recombinant cells for use as medicaments.

The application provides a method and a kit for detecting Staphylococcus aureus. The method embeds multivalent binding probes on microspheres through strain-promoted azide-alkyne cycloaddition reaction, thereby forming microspheres loaded with multivalent binding probes. The microspheres loaded with multivalent binding probes are aggregated in a large size through multivalent binding between the probes and the genome of Staphylococcus aureus, so as to qualitatively and quantitatively identify Staphylococcus aureus through fluorescence. The method not only shortens the detection period and reduces the cost, but also has the advantages of high sensitivity and high specificity.

Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

Described herein are methods to affect binding by a multivalent binding molecule to a FZD receptor and a Wnt co-receptor on a cell wherein binding by the multivalent binding molecule to both FZD receptor and co-receptor on the cell activates a Wnt signaling pathway. Also described herein are multivalent binding molecules comprising a FZD receptor binding domain and a Wnt co-receptor biding domain on either end of an Fc domain that activate a Wnt signaling pathway and methods for their use.

Method for analyzing a sample Su, wherein sample Su comprises an unknown concentration CM_u of a ligand-specific monovalent binding species M and further comprises a ligand-specific multivalent binding species B, the method comprising the steps of an activated sensor surface A1L1 providing step actM comprising providing a sensor surface A1 having a ligand L1 immobilized thereon, wherein the ligand L1 can be monovalently bound by the ligand-specific monovalent binding species M and the ligand L1 can be multivalently bound by the ligand-specific multivalent binding species B, a calibration step calM yielding a monovalent calibration function FA1L1_M, recording in step mesM for the sample Su comprising the unknown concentration CM_u of the ligand-specific monovalent binding species M a sensorgram SGu using the activated sensor surface A1L1, selecting in step selM a response value rA1L1_M_u or the slope of the response value sA1L1_M_u from the sensorgram Su at a selection time tA1L1_M_s, retrieving in step retrM the concentration CM_u of the ligand-specific monovalent binding species M in the sample Su by calculating the concentration form the response value rA1L1_M_u or the slope of the response value sA1L1_M_u using the monovalent calibration function FA1L1_M.

Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

The invention belongs to the field of biological medicine, and particularly relates to a dendritic multi-targeting antibody coupling carrier, an antibody coupling medicine as well as a preparation method and application of the dendritic multi-targeting antibody coupling carrier and the antibody coupling medicine. The dendritic multi-targeting antibody coupling carrier is composed of a targeting module and a drug coupling module, wherein the targeting module has multispecificity and a multivalent binding receptor function; the drug coupling module can realize fixed-point coupling and high drug loading capacity of drugs. The fusion proteins of the two modules can be specifically bound by a spy reaction through spontaneously formed covalent bonds. According to the present invention, the dendritic multi-targeting antibody coupling carrier is further utilized to construct the antibody coupling drug, such that the targeting property of the drug is improved, the relative drug loading capacity of the drug is improved, and the tumor treatment effect of the antibody coupling drug is finally improved.

The present application belongs to the field of bionic technology, and particularly relates to a kind of bionic material loaded with YD monomer and its preparation method and application.The present application utilizes YD monomer composed of tyrosine (Y) and aspartic acid (D) to synthesize a kind of bionic material through reversible addition-fragmentation chain transfer (RAFT) polymerization. The bionic material can produce spatially adjacent binding pockets through the covalent bond of tyrosine (Y) and aspartic acid (D), and can capture two kinds of PTM (phosphopeptide and O-glycopeptide) simultaneously through synergistic interaction. The results of the present application show that the bionic material of YD monomer optimizes charge balance, the neutral phenolic group of Y (pKa ~ 10) and the negatively charged carboxylate of D (pKa ~ 3.9) together reduce the electrostatic repulsion with phosphopeptide, while maintaining target affinity; self-assembled into nanostructured porous framework can expose multivalent binding sites, and significantly improve capacity and selectivity through synergistic effect.

The invention belongs to the technical field of immunochromatography detection, and particularly relates to a time-resolved fluorescence immunochromatography test strip for acute stress syndrome detection as well as a preparation method and application of the time-resolved fluorescence immunochromatography test strip. An IgA antibody is introduced into a detection system to serve as a quality control or recognition element, and the detection specificity and stability are further enhanced. By utilizing the IgA antibody, not only can the compatibility of the reagent and the sample be improved, but also the signal strength can be enhanced through the multivalent binding characteristic of the IgA antibody, so that the detection sensitivity is remarkably improved. Therefore, the subtle fluctuation of cortisol and other hormones in the acute stress state can be more clearly captured, the physiological change of the stress reaction is more obviously reflected, and a more accurate, convenient and reliable tool is provided for early screening, auxiliary diagnosis and curative effect evaluation of mental and psychological diseases.

Provided herein are methods of making and / or identifying multivalent antibody conjugates, e.g., multispecific antibody conjugates, such as bispecific antibody drug conjugates, and conjugated multivalent molecules identified by the methods. In particular, the present invention relates to a method of preparing a multivalent binding polypeptide conjugated to a molecule of interest wherein the method comprises combining a first polypeptide conjugated to the molecule of interest with at least one other polypeptide to form a multivalent binding polypeptide conjugated to the molecule of interest. Intermediates, polypeptide constructs, kits and uses are also provided.

This invention discloses a recombinant protein pPIC9K-17, its preparation method, and its applications, relating to the field of gene recombination technology. The recombinant protein pPIC9K-17 provided by this invention does not strictly follow the (Gly-X-Y)n triple helix repeat pattern of traditional collagen in its core structural unit. The repeating unit sequence includes a "GAIQ" sequence and a sequence containing collagen characteristics, possessing a certain rigid structural basis and containing receptor-binding or signal activation domains for specific growth factors. The direct tandem connection of multiple such units theoretically enables multivalent binding, enhancing affinity and signal activation efficiency with cell surface receptors, thus surpassing the biological functional limitations of single components (pure collagen or pure growth factors) or simple repeating structures. The recombinant protein of this invention not only effectively promotes the proliferation and migration of skin fibroblasts and accelerates collagen synthesis, but also shows clear efficacy in promoting hair regeneration (hair growth), and can be used to prepare products for skin repair and / or promoting hair regeneration.

The present application relates to a kind of multispecific and / or multivalent binding protein for preventing and / or treating HIV infection, it includes at least 3 antigen binding sites, part of antigen binding site is specifically bound HIV-1-gp41 or HIV-1-gp120 or HIV-1-gp160, still part of antigen binding site is specifically bound human CD4 receptor or human CCR5 receptor;The binding protein of the present application has excellent broad-spectrum neutralizing ability in the treatment or prevention of HIV infection, provides a new solution for the problem of drug resistance escape caused by HIV virus variation, in addition, the present application inventor also unexpectedly found that the binding protein of the present application can treat or prevent the tumor-related disease caused by HIV infection.

Provided herein are, inter alia, polypeptides and covalent complexes including the same, with multivalent binding specificity. The polypeptides include an Fc receptor portion / domain (e.g., a CD16 domain, a CD32 domain, a CD64 domain and variants thereof) with a cysteine amino acid substitution capapble of forming a disulfide linkeage with a second cysteine amino acid substitution in an Fc domain (e.g., an Fc domain of IgG1, IgG2, IgG3, IgG4) thereby forming a covalent complex with multivalent binding ability. The compositions provided herein exhibit highly specific binding with reduced or no endogenous Fc receptor binding activity thereby resulting in therapeutics that lack undesirable side effects.

This invention provides a method for screening multivalent binding probes for bacterial detection, comprising the following steps: (1) using an algorithm to screen nucleic acid sequences within the top 1000 range of multiplicity scores for the target bacterial genome; (2) performing secondary screening on the nucleic acid sequences obtained in step (1) to obtain multivalent binding probes for bacterial detection, wherein the secondary screening includes at least one of the following: (2-1) screening nucleic acid sequences with G and C base content in the range of 40%-60%; (2-2) screening nucleic acid sequences in which the maximum continuous length of a single G / C base does not exceed 3 and the maximum continuous length of G-C alternation does not exceed 5; (2-3) screening nucleic acid sequences in which the Gibbs free energy change for forming a hairpin structure and the Gibbs free energy change for forming a homodimer structure are both > -5 kcal / mol. This method is scientific and systematic, and can assist technicians in efficiently and accurately judging and selecting multivalent binding probes suitable for bacterial detection.

The present disclosure provides compositions and methods related to antiviral therapeutics. In particular, the present disclosure provides a highly modular platform useful for the development and production of multivalent biomolecules capable targeting and neutralizing a virus.

The present invention relates to multivalent binding molecules that activate WNT signaling and uses thereof. Described herein are methods of affecting the binding of a multivalent binding molecule to FZD receptors and Wnt co-receptors on a cell, wherein binding of the multivalent binding molecule to both FZD receptors and co-receptors on the cell activates the Wnt signaling pathway. Also described herein are multivalent binding molecules that activate the Wnt signaling pathway comprising a FZD receptor binding domain and a Wnt co-receptor binding domain on either end of an Fc domain and methods of using the same.

Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

The present invention relates to a modular multivalent antigen-binding protein complex, use of the antigen-binding protein complex in medicine and use of the antigen-binding protein complex in the prophylaxis, treatment or diagnosis of a disorder or disease.

The compounds of the present disclosure include a modified dideoxynucleotide triphosphate (ddNPT) comprising a plurality of binding units comprising arms to provide a multivalent binding unit molecule.

Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

The invention belongs to the technical field of biological medicine, and particularly relates to a degradable polymer for loading nuclide, a preparation method and an application method thereof. The degradable polymer for loading nuclide comprises a main chain and a modified chain segment connected to the tail end of the main chain, the main chain is formed by condensation polymerization of HPMDA, RSM and DOTA-OH; the modified chain segment comprises mPEG5k-OH (mPEG (Polyethylene Glycol) 5k-OH. A high-affinity recognition unit or a multivalent binding strategy is introduced, so that the targeting capacity of the small molecule carrier is enhanced. A controllable degradation structure design is adopted, so that the polymer carrier can be efficiently decomposed into harmless small molecules in vivo after finishing targeting and imaging tasks, and the harmless small molecules are discharged. Through an environmental response type bonding or multi-barrier strategy, the carrier is only triggered to be degraded and released in a specific tumor microenvironment, so that the risk of drug leakage is reduced, and radiation damage outside a target and imaging signal loss are reduced. The nuclide imaging function and the tumor treatment function are integrated on the same platform, and diagnosis and treatment integration is achieved.