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Vaccine composition as well as preparation method and application thereof

A vaccine composition and a technology for chicken Newcastle disease virus, applied in the field of vaccine compositions, can solve the problems of increased mortality, slow development, economic loss and the like

Active Publication Date: 2015-01-07
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the slow development of the disease and the long course of the disease, once it is infected in the chicken flock, it is difficult to eradicate it, so it spreads in the chicken flock for a long time, resulting in low feed utilization rate, growth retardation, increased elimination rate, and decreased egg production.
If the pathogen exists in chicken flocks, it is prone to mixed infection, which will aggravate the disease, increase the mortality rate, and cause serious economic losses.

Method used

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  • Vaccine composition as well as preparation method and application thereof
  • Vaccine composition as well as preparation method and application thereof
  • Vaccine composition as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 The preparation of chicken Newcastle disease, infectious bronchitis virus liquid and mycoplasma synoviae bursae bacterial liquid

[0039] 1. Selection of virus strains

[0040] The LaSota strain of the Newcastle disease virus and the M41 strain of the infectious bronchitis virus were used to manufacture this product, both of which were purchased from the China Veterinary Drug Control Institute. Mycoplasma synovia was isolated and identified by the laboratory itself, and it was HN1 strain.

[0041] 2. Virus Fluid Preparation

[0042] 2.1 Preparation of virus solution for chicken Newcastle disease virus La Sota strain vaccine

[0043] 2.1.1 Inoculation

[0044] Take the virus seeds for production and dilute to 10 with sterile physiological saline -3 , Inoculate 10-day-old susceptible chicken embryos through the allantoic cavity, 0.1ml per embryo, seal the pinhole after inoculation, and continue incubation at 36-37°C without turning the eggs.

[0045] 2.1.2 ...

Embodiment 2

[0079] The preparation of embodiment 2 vaccine

[0080] 1 oil phase preparation

[0081] Take 94 parts of white oil for injection, add Siben-806 parts after mixing, add 2 parts of aluminum stearate, add and stir until transparent, autoclave for later use, which is the oil phase.

[0082] 2 Aqueous phase preparation

[0083] Mix the antigens in a sterile container in proportion, add 4% sterilized and cooled Tween-80, and stir while adding, until the Tween-80 is completely dissolved, which is the water phase.

[0084] 3 emulsification

[0085] Take 3 parts of the oil phase and put them in the oil phase tank, start the motor to stir, then slowly add 1 part of the water phase, transfer to the emulsification tank after adding, and then emulsify at 2800r / min for 40 minutes. Add 1% thimerosal before the emulsification is terminated to make the final concentration 0.01%. The specific formula of the vaccine is shown in the table below.

[0086] Table 1 Vaccine composition and cont...

Embodiment 3

[0116] Embodiment 3 Newcastle disease, infectious bronchitis, mycoplasma synovialis triple inactivated vaccine and Newcastle disease, infectious bronchitis double inactivated vaccine+mycoplasma synovialis inactivated vaccine immune side reaction comparison

[0117] Grouping and immunization of 1SPF chickens

[0118] Get 20 SPF chickens of age of 14 days, wherein 10 vaccine 1, 0.3ml / of subcutaneous immunization embodiment 2 preparations, other 10 vaccine 3 (0.3ml / ) and vaccine 4 (0.3ml / ) of subcutaneous immunization embodiment 2 preparations simultaneously 0.3ml / piece). In addition, 10 SPF chickens of the same age were not immunized as controls.

[0119] 2 side effects comparison

[0120] After 14 days of immunization, all chickens were weighed, and the body weight results are shown in Table 6. The results showed that, compared with the control group, the triple vaccine immunization group had no significant difference in body weight at 14 days after injection (ANOVA, P>0.05)...

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Abstract

The invention provides a vaccine composition. The vaccine composition comprises an immune amount of an avian newcastle disease virus antigen, an avian infectious bronchitis virus antigen and an avian mycoplasma synoviae antigen and a carrier. The invention further provides a preparation method and application of the vaccine composition. The immune effect of the trigeminal vaccine is superior to the immune effect of combining newcastle disease-infectious bronchitis inactivated vaccines with mycoplasma synoviae inactivated single vaccine. The invention further provides a vaccine composition which contains a mycoplasma gallisepticum antigen in addition to the three antigens, and can be used for effectively resisting attack of four pathogens.

Description

technical field [0001] The invention relates to a vaccine composition, a preparation method and application thereof, and belongs to the field of bioengineering. Background technique [0002] Newcastle disease (New Castle disease) is a highly contagious and fatal disease caused by paramyxovirus, also known as Asian chicken plague or pseudo chicken plague, often showing symptoms of acute sepsis. The main features are dyspnea, loose stools, nervous disorders, mucosal and serosal hemorrhage, high mortality, and serious harm to the chicken industry. There is no effective treatment yet, so we can only rely on strict disinfection, isolation and vaccination with inactivated vaccines and live vaccines. [0003] Avian infectious bronchitis disease is an acute and highly contagious respiratory infectious disease of chickens caused by infectious bronchitis virus. Chickens of all ages are susceptible, and its clinical characteristics are dyspnea, rales, coughing, mouth breathing, and s...

Claims

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Application Information

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IPC IPC(8): A61K39/395A61K39/02A61K39/17A61K39/215A61P31/04A61P31/14
Inventor 张许科孙进忠李雪锋李成山田克恭
Owner PU LIKE BIO ENG
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