32results about How to "High killing efficiency" patented technology

The invention discloses a serum-free culture system and culture method of NK cells. The invention firstly discloses a serum-free culture system of the NK cells, wherein the serum-free culture system comprises a coating solution, an amplification culture medium, an activation culture medium and autologous plasma; the invention further discloses a culture method for NK cells by using the serum-free culture system. The method for culturing the NK cells by using the serum-free culture system disclosed by the invention reduces the culture cost of the NK cells, can simply, quickly, efficiently and safely obtain a large number of NK cells with high activity, high purity and strong killing ability in vitro, provides a better cell source for clinical application of the NK cells, and promotes further development of an NK cell treatment technology.

The invention belongs to the technical field of termite prevention and control, and discloses an intelligent termite monitoring, early warning and automatic killing device and system.The automatic killing device comprises a controller, a bait frame, a pesticide spraying mechanism and a shell, a containing cavity is formed in the shell, and a plurality of luring through holes are formed in the shell in the shell thickness direction; the bait frame and the pesticide spraying mechanism are both arranged in the containing cavity. The bait frame is provided with a plurality of batten strips and at least one trigger batten. The output end of the pesticide spraying mechanism is located above the batten strips and the trigger battens. The controller is electrically connected with the pesticide spraying mechanism and the triggering battens, and the triggering battens are used for sending a first signal to the controller when the termites enter the containing cavity through the luring through holes and gnaw the triggering battens; the controller is used for controlling the pesticide spraying mechanism according to the first signal so that the pesticide spraying mechanism can spray pesticide liquid to all the batten strips and all the trigger battens. According to the technical scheme provided by the invention, intelligent termite killing is realized, the working intensity of workers is reduced, and the termite killing efficiency is improved.

The invention provides nano-particles for generating CAR-T in vivo and application of the nano-particles, and belongs to the technical field of medical preparations. The nanoparticles are nanoparticles which are coupled with a CD7 antibody and entrapped with mRNA (messenger Ribonucleic Acid); the mRNA is obtained by transcription of a target gene; the target gene comprises a TROP2 single-chain antibody; the nucleotide sequence of the TROP2 single-chain antibody is as shown in SEQ ID NO: 1. The nucleotide sequence of the TROP2 single-chain antibody is optimized, the CAR containing the TROP2 single-chain antibody is constructed, then the CAR is transcribed to obtain mRNA, LNP entrapped with mRNA is prepared, then the CD7 antibody subjected to amino acid sequence optimization is adopted to be coupled with the LNP entrapped with mRNA, the transfection efficiency of the CD7 antibody in T cells is high, the CD7 antibody is injected into a mouse tumor model, and the lifetime of mice can be prolonged; t cells transfected by the T cell transfecting agent have high killing efficiency on HELA cells and LOVO cells.

The utility model discloses a protection device for forestry pest control, which comprises a collection box, a black light lamp is fixedly installed on the top of the inner cavity of the collection box, electric shock nets are fixedly installed on the front side and the rear side of the black light lamp and located in the collection box, and the electric shock nets are electrically connected with a storage battery above the inner cavity of the collection box. Electric air cylinders are fixedly installed on the front side and the rear side of the top of an inner cavity of the collecting box correspondingly, and insect scraping plates are fixedly installed at the output ends of the electric air cylinders. The electric air cylinder and the pest scraping plate are arranged, the electric air cylinder supplies power through the storage battery, when excessive pest bodies adhere to the surface of the electric shock net, the pest scraping plate can be used for scraping and cleaning the pest bodies adhering to the surface, and the cleaned pests fall into the storage box; the pests are directly discharged to the ground by utilizing the guide plate, so that accumulation caused by excessive dead pests can be avoided, rotting and stinking can also be avoided, and the efficiency of killing the pests by the electric shock net is improved.

The invention relates to the technical field of genetic engineering antibodies, in particular to a bispecific antibody combined with BCMA and CD3 as well as a preparation method and application of the bispecific antibody. The invention provides a bispecific antibody which can be combined with BCMA and CD3 at the same time, has a specific targeting effect and can efficiently excite oriented immune response. The bispecific antibody well retains the biological functions of an anti-CD3 antibody and an anti-BCMA antibody, realizes that one bispecific antibody molecule simultaneously has an excellent BCMA and CD3 targeted binding biological function, can build a bridge between tumor cells and immune effector cells, effectively activates the immune effector cells and oriented immune response, and has a good application prospect. The tumor cell killing effect of immune cells is remarkably enhanced, the target cell killing efficiency is high, the ADCC effect is reduced to the maximum degree, high safety is achieved, and good application prospects are achieved in tumor immunotherapy.

The invention discloses a composition and application thereof in resisting multi-drug resistant acinetobacter baumannii, and belongs to the technical field of bacterial infection resistance. The invention provides a composition for resisting multi-drug resistance acinetobacter baumannii. The composition is prepared from emodin, aloe-emodin and rhein, the mass ratio of the emodin to the aloe-emodin to the rhein is 1: 1: 1. The invention also provides an application of the composition in preparation of an antibacterial preparation. According to the composition disclosed by the invention, the sensitivity of the multi-drug-resistant acinetobacter baumannii to polymyxin and carbapenem antibiotics can be recovered again, and the killing time of the polymyxin and carbapenem antibiotics to the multi-drug-resistant acinetobacter baumannii can be shortened; the healing of a mouse skin wound infected by the multi-drug-resistant acinetobacter baumannii can be accelerated. In addition, the compositions of the present invention do not exhibit cytotoxicity at lower concentrations. Therefore, the composition disclosed by the invention can be used for preparing a medicine for resisting multi-drug-resistant acinetobacter baumannii infection.

The invention provides a drug delivery system combined with targeted nasopharyngeal carcinoma treatment and a preparation method and application thereof, and belongs to the technical field of biomedicine.The drug delivery system is characterized in that a liposome is modified by a nucleic acid aptamer S3 specifically targeting nasopharyngeal carcinoma cells, and then an anti-cancer drug adriamycin is entrapped for targeted killing of NPC cells and tissues; besides, NK cells modified by nucleic acid aptamers S3 and PD-L1 are prepared for targeted infiltration of NPC tissues, the NK cells modified by the nucleic acid aptamers S3 and PD-L1 are combined with anti-PD-L1 antibodies to induce'antibody-dependent cytotoxic effect ', and finally, a chemotherapeutic drug targeted delivery system and immunotherapy are organically combined to form a multi-mode therapeutic scheme. And while multi-angle and multi-layer striking is carried out on tumors, the toxic and side effects on normal cells and tissues can be reduced, and the treatment effect on nasopharyngeal carcinoma patients is remarkably improved.

The invention discloses cysteine mediated Hf / Cu bimetal synergistic self-assembled nano-particles as well as a preparation method and application of the cysteine mediated Hf / Cu bimetal synergistic self-assembled nano-particles. According to the method, cysteine is used as a multifunctional ligand, carboxyl and sulfydryl of cysteine are in bridge connection with metal through coordination, ligand chelation and assembly guiding effects are synchronously exerted, bimetallic ions are induced to be cooperatively self-assembled in an alkaline system (pH is 9-10), and metal cysteine self-assembled nanoparticles with uniform structures are formed through in-situ nucleation and multistage aggregation. According to the method, the ligand function and the bimetallic synergistic effect are accurately matched, the synthesis process is mild (room temperature), the operation is simple and convenient, and the product is excellent in dispersity and stability. Meanwhile, a close coordination structure constructed by cysteine provides a key channel for energy / electron transfer from sensitizing metal to Cu under X-ray irradiation, and lays a structural foundation for subsequent catalytic effect enhancement.

The invention belongs to the technical field of biology, and particularly relates to an immune engineering cell for expressing a discontinuous truncated functional region of GM-CSF and application. The invention firstly provides a CAR-NK cell for expressing a discontinuous truncated functional region of GM-CSF, and the CAR-NK cell expresses a novel chimeric antigen receptor structure; the novel chimeric antigen receptor structure comprises a discontinuous truncated functional domain fragment of a GM-CSF protein and a chimeric antigen receptor; the non-continuous truncated functional domain fragment of the GM-CSF protein comprises an A helix fragment, a C helix fragment and an exon 2 fragment. The invention also provides a CAR-T cell for expressing the discontinuous truncated functional region of the GM-CSF as well.

The utility model discloses an insect killer, and aims to solve the problem of low insect killing efficiency of an insect killer in the prior art. The technical problem is solved through the following technical scheme that the insect killing device comprises a controller box and a base, a luring lamp is installed on the controller box, a plurality of layers of high-voltage insect killing nets are arranged outside the luring lamp, and the diameters of meshes of the high-voltage insect killing nets are gradually increased from inside to outside. The trapping lamp is used for trapping insects to gather, a plurality of layers of high-voltage deinsectization nets are arranged, meshes of the outer high-voltage deinsectization net are large, the insects with large sizes penetrate through the meshes of the outer high-voltage deinsectization net, and the small insects enter the inner high-voltage deinsectization net to be killed. Therefore, the insect killing efficiency can be effectively improved.

The utility model relates to the technical field of sorghum planting protection device, more particularly, disclose a kind of for sorghum pest's trapping and killing device, it include: arc plate;Box, be set in the below of arc plate, connect the both sides of arc plate;Turnover plate, rotatably be set in first baffle and second baffle, between arc plate and box;Strip-shaped hole, be set in the side of arc plate;Breather, be set in the side of arc plate away from strip-shaped hole;Lure component, set in the side of breather away from turnover plate, for luring sorghum midge;Driving component, for driving turnover plate rotation;Killing assembly, for spraying insecticide to box;By using the utility model, sorghum midge can be captured and killed all-weather, compared with insecticidal lamp, the utility model uses condition is not limited by external light intensity, in rainy day also can continuously kill sorghum midge operation, can effectively improve the killing efficiency of sorghum midge.

The application provides an ultrasonic response piezoelectric ionic hydrogel and application thereof, and the hydrogel is formed by cross-linking and solidification of a precursor solution under ultraviolet irradiation; components of the precursor solution include a photosensitive polymer, an ionic polymer, a photoinitiator and a solvent; the photosensitive polymer includes any one or a combination of at least two of polyether F127 diacrylate, polyhydroxyethyl methacrylate, polyethylene glycol dimethyl acrylate, methacrylated gelatin or methacrylated collagen; and the ionic polymer includes any one or a combination of at least two of epsilon-polylysine, polyethyleneimine hydrochloride, polyallylamine hydrochloride, polyquaternary ammonium salt-10 or cationic guar gum. The application uses ultrasonic waves as an external remote driving signal to non-invasively and painlessly activate the piezoelectric ionic effect of the hydrogel, generate detectable periodic voltage and current signals in the hydrogel, and has excellent treatment effect on acne and infected wound repair.

The invention discloses a webpage virus detection method, device and equipment and a storage medium, and the method comprises the steps: when it is monitored that a preset type file is loaded by any process, hanging up any process and setting a hook in the preset type file, the hook being used for obtaining a script command to be executed in the preset type file; running of any process is recovered, and when any process executes the command line containing the hook, virus detection is conducted on the script command to be executed. The hook is arranged at the position, used for executing the script command line, in the preset type file, and when the process is loaded to the command line where the hook is arranged, the script command to be executed is obtained and subjected to virus detection; the hook is arranged in the preset type file for compiling the to-be-executed script command to obtain the to-be-executed script command and perform virus detection on the to-be-executed script command, so that the process of performing virus detection on the to-be-processed script is simplified, and the virus searching and killing efficiency is improved.

The utility model discloses an insect condition forecasting device which comprises a bottom plate, the top of the bottom plate is fixedly connected with a trap lamp, the top of the bottom plate is symmetrically and fixedly connected with supporting plates located on the outer side of the trap lamp, the tops of the supporting plates are fixedly connected with a top plate, the interior of the top plate is rotationally connected with a rotating cylinder, and the outer side of the rotating cylinder is symmetrically and fixedly connected with impact plates. The insect killing device has the advantages that the insect trapping lamp can be started to lure insects, the rotating cylinder is rotated to drive the impact plate to rotate, and the conductive nets are embedded in the rotating cylinder and the impact plate, so that the insect killing efficiency can be greatly improved, the situation that the insects escape after being impacted by the impact plate is avoided, and the practicability of the device is improved.

The invention relates to the field of sewage treatment, and discloses an intelligent targeted virus disinfection hospital sewage disinfection process which comprises the following steps: S1, preparing ozone micro-nano bubbles through a micro-nano bubble generator, and injecting ozone gas into sewage to form micro-nano bubbles; s2, adding an oxidizing agent into the sewage containing the ozone micro-nano bubbles to destroy lipid envelopes of the viruses; s3, degrading a protein shell of the virus through a synergistic effect of hydroxyl free radicals and a chemical modifier; s4, destroying viral nucleic acid by combining ultraviolet irradiation and a catalyst; s5, controlling generation of by-products in the reaction process; and S6, after water outlet detection is carried out, water drainage is completed. Through multi-layer structure staged treatment of intelligent targeting viruses, lipid envelope destruction, protein shell degradation and nucleic acid thorough destruction are combined with synergistically optimized oxidation, modification and degradation mechanisms, so that the virus killing efficiency is remarkably improved, and the treatment comprehensiveness and accuracy are ensured.

The utility model discloses a portable rice seed soaking disinfection device, including cylinder, the top of cylinder is equipped with soaking area, and the storage area is equipped with separation component in the soaking area, and separation component drive end extends to the inside of storage area, and the outer wall of cylinder one side is equipped with drain valve, and drain valve one end extends to the inside of soaking area, and separation component includes porous cylinder, installs the cylinder, and a plurality of micropore is equipped with in the outside of porous cylinder, and the bottom of porous cylinder is installed in the inside of soaking area through bearing, and installs the cylinder and is installed in the inside of storage area through bolt, and the inside one end center of installing the cylinder is equipped with drive motor through bolt, the utility model discloses the cooperation design of porous cylinder and drive motor can be fast centrifugal removal seed surface residual liquid after disinfection, and cooperate drain valve and discharge residual liquid fast, effectively avoid the mildew problem of traditional device because of moisture accumulation, improve seed storage security.

The present application provides a method and device for detecting and killing fileless Trojans, which relates to the field of information security and can also be used in the financial field, including: running a Java Agent engine and obtaining a stack call log and class bytecode information of a called program in a Web server application; determining the risk level of the called program according to a preset detection rule, the stack call log and the class bytecode information; determining the class file containing the fileless Trojan in the called program according to a preset detection and killing strategy and the risk level, and detecting and killing the fileless Trojan in the class file. The present application can realize the automatic detection and killing of fileless Trojans and protect the safe operation of Web applications.

This invention belongs to the field of pest control and biomaterial application technology, specifically relating to a termite bait based on cellulose waste and its high-temperature, high-pressure preparation process and application. The bait uses sugarcane bagasse powder and bark powder as the main cellulose matrix, supplemented with glucose, xylose, casein, and granulated sugar as attractants, and incorporates a chitosan-graphene oxide composite nano-insecticide. This bait exhibits high stability in humid environments, is not prone to mold, and maintains its attractant properties and efficacy for a long period. Indoor and buried tests have verified that the prepared bait significantly increases the termite feeding rate and mortality rate compared to the control group, demonstrating a comprehensive advantage of strong attractant properties, slow-release effect, and environmental friendliness.

The invention provides a preparation method and application of a multifunctional bimetallic nano enzyme, and belongs to the technical field of inorganic nano therapeutic agents. The preparation method comprises the following steps: performing hydrothermal reaction on tyrosine, ferric trichloride, cobalt nitrate and sodium hydroxide to synthesize CoFe2O4; the method comprises the following steps: adding a dimethyl sulfoxide solution of mPEG into pure polylipoic acid, dialyzing, and freeze-drying to obtain PALA-mPEG; adding a dimethyl sulfoxide solution of JUG into the PALA-mPEG, carrying out dialysis, and carrying out freeze-drying, so as to obtain PALA-mPEG-JUG; the preparation method comprises the following steps: dispersing CoFe2O4 in deionized water, adding PALA-mPEG-JUG, and carrying out a reaction so as to obtain CFO-coated PPJ; the CFO coated PPJ prepared by the invention is a tumor microenvironment self-adaptive material combining a modified polymer chain and a bimetallic nano-enzyme, and can be used in the fields of enhanced chemical kinetics therapy, multifunctional antitumor agents, diagnosis and treatment integration and the like.

The invention provides an HPV (human papillomavirus) vaccine carrying a CAR (chimeric antigen receptor) structure as well as a preparation method and application thereof, and belongs to the technical field of medical preparations. The CAR structure comprises E7 single-chain antibodies aiming at HPV16, 18, 31 and 33 subtypes and E7 single-chain antibodies aiming at HPV45, 52, 56, 58 and 59 subtypes; the nucleic acid artificial sequence of the E7 single-chain antibody aiming at the HPV16, 18, 31 and 33 subtypes is as shown in SEQ ID NO.5 in a sequence table; the nucleic acid artificial sequence of the E7 single-chain antibody aiming at the HPV45, 52, 56, 58 and 59 subtypes is shown as SEQ ID NO.8 in a sequence table. The prepared HPV vaccine carrying the CAR structure effectively captures CD8 + T cells in a body, forms CAR-T cells, effectively reduces the HPV virus titer in the body of a mouse, can resist infection of various HPV virus subtypes at the same time, and has a remarkable protection effect on the mouse.

The invention discloses a surface glycosylation modified impregnation liquid and a preparation method thereof, an antibacterial fiber, an antibacterial fabric and application, and belongs to the technical field of antibiosis. According to the preparation method, a reactive main chain polymer and amino sugar are adopted, steeping liquor is prepared through step-by-step reaction or one-pot reaction, and then glycosylation modification is conducted on the surface of the nano-antibacterial fiber / fabric. On the basis of the nano antibacterial fiber, the fiber surface is subjected to glycosylation modification, the balance between the biological safety and the antibacterial activity of the nano antibacterial fiber material is realized, and the graphene fiber capable of selectively capturing and enriching pathogenic microorganisms such as bacteria and the like is obtained by utilizing the specific binding of a characteristic sugar chain to the bacteria; furthermore, the killing efficiency of the nano-antibacterial fiber on dispersed pathogenic microorganisms in the environment is greatly improved, the problem of space limitation of a nano-antibacterial material in contact antibiosis is effectively solved, and the material is suitable for preparing active bacterium targeting type drug-resistant bacterium infection wound dressings.

The application belongs to the technical field of medical materials and relates to a bacteriostatic suture for surgical suturing and a preparation method thereof. The preparation method comprises the following steps: 2-amino-5-acetylpyridine is dissolved in a hydrobromic acid glacial acetic acid solution, an acetic acid solution containing bromine is added, and an intermediate 1 is obtained through reaction; the intermediate 1 is added to tetrahydrofuran, triethylamine and acetic anhydride are added, and an intermediate 2 is obtained through reaction; the intermediate 2 and thiourea are added to anhydrous ethanol, and an intermediate 3 is obtained through reaction; the intermediate 3 and p-nitrobenzaldehyde are added to anhydrous methanol, and an intermediate 4 is obtained through reaction; phosphorus oxychloride is added to N,N-dimethylformamide, and the intermediate 4 is added to obtain an intermediate 5 through reaction; the intermediate 5 is dissolved in a mixed solvent, and cystamine is added to obtain an antibacterial agent; and the medical silk suture is immersed in dimethyl sulfoxide containing the antibacterial agent, and is subjected to ultrasonic treatment, soaking, rinsing and drying to obtain the bacteriostatic suture. The bacteriostatic suture has the advantages of multi-mechanism synergistic enhanced bacteriostatic performance, an environment-responsive active release mechanism and excellent interface affinity.

The invention discloses an application of cholesterol synthetase in preparation of a product for enhancing the killing function of CAR-T cells and derivatives thereof, the cholesterol synthetase is SQLE or LSS, and the product enhances the killing function by separately knocking out the SQLE or separately overexpressing the LSS in the CAR-T cells and the derivatives thereof. According to the application, a cholesterol synthesis key enzyme SQLE is knocked out or LSS is overexpressed, so that the killing ability of CAR-T cells and derivatives thereof is remarkably enhanced.

The utility model discloses a variety of pest detection and trapping equipment based on light trapping technology, including roof and bottom plate and infrared sensor, infrared sensor installs in the bottom plate side wall, install the electric network between roof and bottom plate, the sliding groove is combined and slides in the outer wall of electric network, the fixed plate is rotatably connected in the electric network, the outer wall sliding connection of fixed plate has connecting rod, the fixed connection of connecting rod end has the brush plate, the brush plate is combined and slides in the inner wall of electric network, the adjusting mechanism of adjusting sliding groove position is installed to the outer wall of rotating plate, the outer wall of connecting rod is provided with the elastic component of the drive brush plate closely combined inner wall of electric network. The utility model discloses through the cooperation of brush plate and scraper, realizes the synchronous removal of the inner and outer wall of electric network insect cadaver, and the brush plate is combined with the inner wall under the action of spring, and the scraper adjusts the wear gap through the threaded rod, ensures long -term stable cleaning effect, avoids the influence of insect cadaver accumulation on the conductivity of electric network, and improves pest killing efficiency.

The invention discloses a traditional Chinese medicine compound cell culture fluid and application thereof. The traditional Chinese medicine compound cell culture fluid comprises a basic culture medium, and 1 L of the basic culture medium contains 2-10 mg of notoginsenoside R1, 1-5 mg of notoginsenoside Rb1, 1-5 mg of notoginsenoside Rg1, 0.1-1 mg of notoginsenoside Rg3, 0.5-2 mg of notoginsenoside Re, 1-6 [mu] g of shikonin, 5-15 [mu] g of cordycepin and 1-20 mg of basil polysaccharide. In the traditional Chinese medicine compound cell culture fluid, notoginsenoside Rg3 can be combined with shikonin, so that the cytotoxicity can be enhanced, cord blood NK cells are promoted to be differentiated to mature functional phenotypes with high cytotoxic activity, and the killing efficiency of the NK cells on various tumor target cells is remarkably improved; the notoginsenoside Rb1 is combined with the basil polysaccharide, so that the NK cells can be driven to expand and grow, the activity of the NK cells can be maintained, and the cell purity can be improved, so that the persistence of the cell activity can be enhanced, and the tumor killing rate of the NK cells can be improved.

This invention belongs to the field of wastewater treatment technology, specifically a method for treating medical wastewater by synergistically removing new pollutants and pathogenic microorganisms. The method includes the following steps: S1, Pretreatment: Inorganic flocculants are added to the medical wastewater to promote the aggregation and sedimentation of suspended particles and colloids; S2, Oxidation Treatment: A composite agent is added to the pretreated medical wastewater while a direct current electric field is applied. The composite agent includes potassium persulfate, aminosulfonic acid, sodium chloride, sodium bromide, surfactant, pH buffer, composite corrosion inhibitor, anti-caking agent, and anhydrous sodium sulfate. After dissolving, the composite agent generates a strong oxidizing component that mineralizes and decomposes organic matter while killing pathogens; S3, Biochemical Treatment: The oxidized medical wastewater is then subjected to biochemical treatment to remove ammonia nitrogen and small molecule organic matter. This invention can effectively decompose various new pollutants and inactivate pathogenic microorganisms, thus improving the treatment effect.

The present invention belongs to the field of medicine and biology, and specifically relates to a dual-target CAR vector combined with EpCAM and MSLN single-chain antibodies, a construction method thereof, and an application in ovarian cancer. The dual-target CAR vector combines EpCAM single-chain antibodies and MSLN single-chain antibodies, and the specific composition structure of CAR is CD8leader-EpCAM scFv-(G4S)5-MSLN scFv-CD8α-CD28-CD137-CD3ζ. CAR-T cells (Chimeric Antigen Receptor T-Cell) containing the therapeutic vector are obtained by viral infection, and by expressing the CAR structure, ovarian cancer cells that highly express EpCAM and MSLN tumor-associated antigens can be targeted for recognition and killing.

The invention relates to the technical field of cell culture, in particular to a culture medium for NKT cells and a use method of the culture medium. The invention provides a culture medium for NKT cells and a use method of the culture medium. The NKT cells are prepared from PBMC cells separated from peripheral blood; the separated PBMC cells are resuspended through a culture medium and then added into the culture medium to be subjected to primary culture and then subculture, and growth active components are added at set intervals in the subculture process, so that the induction efficiency of the NKT cells can be greatly improved, amplification of the NKT cells is facilitated, and the killing efficiency of the NKT cells on cancer cells can be remarkably improved.

The invention relates to a detection device and method for determining the killing capacity of in-vitro immune cells. The device comprises an input module, a monitoring module, an image recognition module, a processor and an output module. According to the method, the killing ability of the immune cells is evaluated based on an artificial intelligence model, the artificial intelligence model is combined with real-time phenotypic signs and number changes of tumor targeted cells and immune cells to judge the killing ability of the immune cells, and specifically, the killing ability of the immune cells is judged according to the number changes of the tumor targeted cells. The evaluation method comprises the following steps: firstly, calculating the immune cell proliferation amount Mn, the tumor cell killing amount Qn, the cytokine release amount Fn and the immune cell toxicity grade Dn according to the weight in a processor, and then obtaining the evaluation result of each stage by matching with normal distribution data of the tumor targeted cell survival cycle tn and combining with the weight in the processor.

An insect killing device for a tobacco field relates to the technical field of insect killing of the tobacco field and comprises an insect killing box and a baffle plate, the insect killing box is of a box body structure, four openings are formed in the periphery of the outer wall of the insect killing box, the openings are communicated with the inner wall face of the insect killing box, the baffle plate is arranged on the inner wall face of the insect killing box and is opposite to the openings, and the baffle plate is arranged on the outer wall face of the insect killing box. An opening is formed in the outer side of the insect killing box, a shielding plate is arranged on the inner end face of the insect killing box, the shielding plate is of a cover body structure, four inlets are formed in the position from the periphery of the outer wall to the inner end face of the shielding plate at intervals, the opening and the shielding plate are arranged on the outer side and the inner end face of the insect killing box respectively, and the inlets in multiple directions are further formed in the shielding plate, so that when the insect trapping lamp works, the insect killing effect is improved; by means of the design, pests can make contact with the inlets in multiple directions, the pest entering speed is increased, the situation that a large number of pests are attached to the outer portion of the baffle plate is avoided, and therefore the pest killing efficiency is improved.