Suspension chip detection method for simultaneously detecting hepatitis B virus, hepatitis C virus, treponema pallidum and human immunodeficiency virus pathogens

A suspension chip, AIDS technology, applied in the field of immunological detection, can solve the problems of complicated procedures, time-consuming, heavy workload, etc., and achieve the effects of strong specificity, accurate and reliable detection results, and small sample consumption.

Inactive Publication Date: 2011-04-20
中华人民共和国陕西出入境检验检疫局
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Problems solved by technology

[0003] The purpose of the present invention is to overcome the defects of the existing pathogen detection method, such as complex procedures, heavy workload, time-consuming, and insufficient sensitivity

Method used

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  • Suspension chip detection method for simultaneously detecting hepatitis B virus, hepatitis C virus, treponema pallidum and human immunodeficiency virus pathogens
  • Suspension chip detection method for simultaneously detecting hepatitis B virus, hepatitis C virus, treponema pallidum and human immunodeficiency virus pathogens
  • Suspension chip detection method for simultaneously detecting hepatitis B virus, hepatitis C virus, treponema pallidum and human immunodeficiency virus pathogens

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Embodiment Construction

[0034] In the present invention, the plastic coded microspheres purchased from the U.S. Luminex Company (the plastic coded microspheres with a diameter of 5.6 μm) are used as a carrier, and the monoclonal hepatitis B surface antibody, HIV1 gP41 antigen, HCV NS3 antigen, and syphilis TP15 antigen are respectively coated on 4 On microspheres with different codes, four types of suspension chips were prepared for use.

[0035] The suspended chip detection method of the present invention comprises the following contents:

[0036] Firstly, the above-mentioned suspension chip is prepared, which specifically includes the following steps:

[0037] 1) Activation of encoded microspheres:

[0038] 1.1) First, take 3000-5000 coded microspheres into a 1.5mL centrifuge tube, centrifuge at 12000-14000g for 3-5min, preferably 14000g for 4min, carefully suck out the supernatant and discard it;

[0039] 1.2) Add 100 μL of pH 7.4, 0.05% TWEEN-20 PBS buffer to suspend, oscillate for 30 seconds, ...

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Abstract

The invention relates to a suspension chip detection method for simultaneously detecting hepatitis B virus, hepatitis C virus, treponema pallidum and human immunodeficiency virus pathogens. Plastic color coded microspheres with diameter of between 5.5 and 6.5 mu m are taken as vectors for a suspension chip; and the carriers are coated with monoclonal hepatitis B virus surface antibodies, HIV1, gP41 antigens, HCV NS3 antigens or treponema pallidum TP15 antigens. Through the suspension chip, the hepatitis B virus, hepatitis C virus, treponema pallidum and human immunodeficiency virus pathogens in a sample to be detected can be very conveniently and quickly detected at the same time; and the method has the advantages of accurate and reliable detection result, high specificity, small using amount of samples and high-throughput detection.

Description

technical field [0001] The invention belongs to the technical field of immunological detection, namely a suspension chip detection method for co-detection of hepatitis B, hepatitis C, syphilis and AIDS. Background technique [0002] With the continuous increase of my country's opening to the outside world, the number of people entering and leaving the country has increased significantly, and the task of monitoring infectious diseases at border ports has become more and more arduous. The security system poses severe challenges, and the frontier health and quarantine defense line is under enormous pressure and risks. The reality urgently calls for accurate, reliable, sensitive and efficient detection technology to play a role in building a safe and reliable frontier health and quarantine defense line. Suspended chip technology is an ideal choice to meet this actual demand. Aiming at the infectious diseases hepatitis B, hepatitis C, syphilis, and AIDS that are routinely monitor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/576G01N33/571G01N33/569G01N33/546G01N33/531
Inventor 张会孝
Owner 中华人民共和国陕西出入境检验检疫局
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