Suspension chip detection method for simultaneously detecting hepatitis B virus, hepatitis C virus, treponema pallidum and human immunodeficiency virus pathogens
A suspension chip, AIDS technology, applied in the field of immunological detection, can solve the problems of complicated procedures, time-consuming, heavy workload, etc., and achieve the effects of strong specificity, accurate and reliable detection results, and small sample consumption.
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[0034] In the present invention, the plastic coded microspheres purchased from the U.S. Luminex Company (the plastic coded microspheres with a diameter of 5.6 μm) are used as a carrier, and the monoclonal hepatitis B surface antibody, HIV1 gP41 antigen, HCV NS3 antigen, and syphilis TP15 antigen are respectively coated on 4 On microspheres with different codes, four types of suspension chips were prepared for use.
[0035] The suspended chip detection method of the present invention comprises the following contents:
[0036] Firstly, the above-mentioned suspension chip is prepared, which specifically includes the following steps:
[0037] 1) Activation of encoded microspheres:
[0038] 1.1) First, take 3000-5000 coded microspheres into a 1.5mL centrifuge tube, centrifuge at 12000-14000g for 3-5min, preferably 14000g for 4min, carefully suck out the supernatant and discard it;
[0039] 1.2) Add 100 μL of pH 7.4, 0.05% TWEEN-20 PBS buffer to suspend, oscillate for 30 seconds, ...
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