244results about How to "Long-term storage and use" patented technology

The invention discloses a fluorescent probe for detecting a biologic thiol and a preparation method and a usage method thereof. The fluorescent probe for detecting the biologic thiol consists of two parts: namely, a 2, 4-bi-nitrobenzene sulfonyl group which is a recognizing group and a boron difluoride-dipyrryl methane (BODIPY) derivative which is an information reporting functional group. The molecule of the probe can simply and quickly enter a living cell, generates a specificity reaction with the thiol in the cell, and causes to obviously enhance fluorescence intensity, so that the fluorescent probe further can be used for the fluorescence detection and the imaging of the active thiol in the living cell. The fluorescent probe has good stability, can be stored and used for a long time, is applicable to various environments in which the living cell grows, and has higher detection sensitivity for the thiol, strong anti-interference capability, excellent selectivity and no action on other common biologic interfering molecules. The fluorescent probe can simply enter the living cell and a living tissue; the single recognition of the thiol in a biologic system can be achieved effectively; and therefore, the fluorescent probe can be used for the fluorescence imaging of the living cell.

The invention discloses a preparation method of an adsorbent for removing phosphorus and heavy metal anions in water as well as an application method, and belongs to the fields of adsorbent technology and environmental pollution remediation. The adsorbent is magnetic water hyacinth biochar, and is prepared by the following steps: water hyacinth is dried and pulverized into powder; a proper amount of the water hyacinth powder and a mixed solution containing Fe2+ and Fe3+ are mixed with continuous stirring, and sodium hydroxide is added dropwisely till the pH value is about 10; after filtering, anaerobic pyrolysis is carried out at 250 DEG C or 450 DEG C, and washing, pumping filtration and drying are carried out in order to obtain the magnetic water hyacinth biochar. The application method is as follows: the biochar is added into waste water containing phosphor, arsenic, antimony and chromium, the initial concentration is 0.05-0.5mM, the dosage is 5g/l, a reaction is carried out for 24 hours at 25 DEG C, and the oscillation rate is 150r/min. The adsorbent is advantageous in that the adsorbent has a large capacity for adsorbing phosphor and heavy metal anions in water, the adsorbent is not easy to agglomerate, and after adsorption, the adsorbent is easy to separate and recycle.

The present invention relates to a new-type singlet oxygen europium fluorescent probe and its application. It is a coordination compound formed by using trivalent europium ion Eu3+ and 2, 2í»:6í»2íŒ-ditripyridine skeleton structure ligand containing functional group. Said invention also provides the structure formula of the described ligand. Said coordination compound can be used for making fluorescent determination of singlet oxygen.

The invention belongs to the technical field of biological detection, which particularly relates to a fluorescent probe which can be applied in measuring active mercapto image in living cells and the application. The fluorescent probe is 2-(2-hydroxyphenyl) hydroxyphenyl compound which is decorated by maleimide or maleimide acid, the structure of the compound is N-{3-(2- benzimidazolyl)-4-hydroxyphenyl}maleimide and N-{3-(2- benzimidazolyl)-4-hydroxyphenyl}malemide acid. The compound can simply and rapidly get into living cells, which has a reaction with free active mercapto group in cells, and fluorescence intensity of the compound is led to be obviously enhancing, thereby the fluorescent probe can be used in fluorescent measurement or fluorescent image of fluactive mercapto group (such as glutathione and the like) in living cells.

The invention provides a type of fluorescent probes for detecting formaldehyde. The probes have the structures shown as formulas I to IV. Benzene ring correspondingly substituted coumarin is firstly subjected to backflow reaction with Lawson reagents in toluol; an obtained product continuously performs backflow reaction with hydrazine hydrate in ethanol. The fluorescent probes per se provided by the invention do not have fluorescence in the water solution, but can take a specific fast reaction with formaldehyde to produce products with strong fluorescence, so that the specific sensitive detection and quantitative analysis of formaldehyde is realized. The stability is high; the long-time storage use can be realized; the detection signal-to-noise ratio is high; the sensitivity is high; the excellent selectivity is realized; the specific formaldehyde detection in complicated biological samples can be realized; good biomembrane permeability is realized, so that the fluorescent probes can be used for formaldehyde detection in living cells; the detection reaction is fast; the detection result can be given within 1 minutes for formaldehyde in the water solution; during the formaldehyde detection, the formaldehyde concentration in the environment cannot be influenced. The general molecular formulas of the probes provided by the invention are shown as the accompanying drawing.

The invention discloses a malononitrile derivative near-infrared hydrogen sulfide fluorescent probe as well as a preparation method and application thereof. The probe has a structure represented by aformula I shown in the description. The preparation method comprises the following steps: firstly, mixing isophorone, malononitrile and piperidine, performing heating reflux under the protection of aninert gas, performing purification, mixing the obtained purified product, p-hydroxybenzaldehyde and piperidine, performing heating reflux under the protection of an inert gas, performing purification, mixing the obtained purified product, urotropine and trifluoroacetic acid, performing heating reflux under the protection of an inert gas, performing purification, finally mixing the obtained purified product, 2,4-dinitrobenzenesulfonyl chloride and triethylamine, performing a reaction under stirring at room temperature, and performing purification to obtain the fluorescent probe. The fluorescent probe provided by the invention has large Stokes shift (229 nm), a longer fluorescence emission wavelength (653 nm) and high sensitivity, can be used for specific detection of biological hydrogen sulfide, and has good biofilm permeability and low cytotoxicity; and the fluorescent probe has a simple synthetic route, a high yield and large practical values.

The invention relates to a rapid high-selectivity hypochloric acid fluorescence probe and an application thereof. The probe is 4-hydroquinone based naphthalimide compounds, and the compounds can be used to detect hypochloric acid as a hypochloric acid fluorescence probe. The probe has at least one of the following technical effects: highly-selectively identification of hypochloric acid/hypochlorite ions; rapid response to hypochloric acid/hypochlorite ions; instant detection of hypochloric acid/hypochlorite ions; stable properties and long-term preservation and use; facilitation of detection of hypochloric acid under physiologic level conditions; and strong anti-interference ability.

The invention relates to a rapid high-selectivity fluorine ion colorimetric probe and a preparation method therefor. The probe not only can highly selectively recognize fluorine ions, but also can quantitatively and qualitatively analyze the fluorine ions by way of observation in naked eyes. The probe can be used for quickly responding to the fluorine ions, so that the fluorine ions can be immediately detected. The probe is stable in property, and can be stored and used for a long time. In addition, the probe further can be used for quantitatively analyzing the fluorine ions by virtue of absorption spectroscopy and has relatively strong anti-interference capacity. The probe is synthesized by a room temperature method, requires only one step reaction, and is simple to synthesize.

The present invention relates to a fast and highly selective mercury ion fluorescent probe. The mercury ion fluorescent probe is a sulfocarbonate compound, and the structural formula is as shown in the specification. The mercury ion fluorescent probe can achieve at least one of the following technical effects: highly selective identification of mercury ions; rapid response to the mercury ions; large Stokes shift; stable nature, long term preservation and use; and strong anti-interference ability.

The invention aims to provide an ammonia adsorbent and a preparation method thereof. The technical scheme adopted by the invention is as follows: furnace slag of coal is smashed, a certain amount of furnace slag powder is taken, a phosphoric acid solution with the concentration of 10-50% is added, impregnating is carried out at the room temperature for 3-24h, and then drying is carried out; after drying, solid matters are heated from the room temperature to the preset temperature (300-700 DEG C), the temperature is kept for 2h, then the heated solid matters are cooled to the room temperature, and the final product is taken out, and is washed with distilled water to be neutral, dried, and bottled for standby, so that the ammonia adsorbent can be obtained.

The invention provides a barium and strontium scale removing agent and a preparation method thereof. The scale removing agent is prepared from the following raw materials in percentage by weight: 10-15% of hydrochloric acid, 2-5% of hydrofluoric acid, 2-5% of chelating agent, 2-5% of scale inhibitor, 0.5%-1% of corrosion inhibitor, 0.5%-1% of surfactant, 0.1-0.3% of synergist, 0.2%-0.4% of modifier and the balance water. The scale removing agent raw materials are wide in resource and low in price, the prepared scale removing agent is stable in scale removing performance, good in temperature resistance, long in service life, capable of being stored and used for a long term, and high in scale dissolution capability, a novel method is provided for the aspect of oil field acid liquor insolublescale treatment, and the barium and strontium scale removing agent has good application prospects in the aspect of water well decompression and augmented injection.

The invention belongs to the technical field of biological detection and relates to a novel method for detecting the activity of hydrogen sulfide synthetase by using a hydrogen sulfide fluorescence probe as well as an application approach of the method. The method is suitable for detection of the activity of hydrogen sulfide synthetase such as CBS, CSE and CL, and comprises the following steps: shearing a tissue sample into small blocks, grinding, performing cell lysis in a tissue cell lysis solution, performing protein quantification on a lysis product, as the drawings, adding a reaction system (comprising the tissue cell lysis product, L-cysteine and 5-pyridoxal phosphate) into a reaction pool on an outer ring of a reaction bottle, and adding an absorption system (comprising operating fluid of the H2S fluorescence probe containing a reaction adjuvant cetrimonium bromide) into an absorption pool on an inner ring of the reaction bottle, wherein H2S gas molecules generated in the reaction system are combined with the fluorescence probe in the absorption system, the system can be used for fluorescence assay and fluorescence imaging, the H2S content is quantitatively detected, and the activity of the H2S synthetase is intuitively reflected. The invention can provide a good method for research of H2S-related signal paths and significance and diagnosis of H2S in disease processes.

The invention relates to a fast high-selectivity super-sensitive nickel ion colorimetric fluorescent probe and a preparation method thereof. Specifically, the probe provided by the invention is an allyl carbonic ester compound and can be used as a nickel ion colorimetric fluorescent probe for detecting nickel ions. The probe can realize at least one of the following technical effects: high selectively recognizing the nickel ion, quickly responding to the nickel ion, high sensitively analyzing the nickel ion, realizing the naked eye colorimetric analysis for the nickel ion and having stable properties, long-term storage and use and higher anti-jamming capability.

The invention discloses an etchant for observation and energy spectrum analysis of the sigma phase through a scanning electron microscope. The etchant is formed through mixing the following raw materials in percentage by mass: 1-12% of copper(II) sulfate pentahydrate, 8-21% of sodium chloride, 18-41% of phosphoric acid, 0.1-7.5% of absolute ethyl alcohol and 19-72% of distilled water; during the etching process of austenite heat-resistance stainless steel, the etchant composed of copper(II) sulfate pentahydrate, sodium chloride, phosphoric acid, absolute ethyl alcohol and distilled water can effectively restrain an etch pit formed through excessive corrosion, dissolving and falling; the scanning electron microscope can observe that the morphology of the sigma phase in the austenite heat-resistance stainless steel is complete and clear, the situation that an energy spectrometer can be adopted to carry out precise composition detection and analysis on the sigma phase is ensured, and a significance is played in analyzing and determining the failure cause of the austenite heat-resistance stainless steel material; and the etchant is simple in composition, stable in etching effect, and high in reproducibility, can be stored and used for a long time, and is an ideal etchant for observation and energy spectrum analysis of the sigma phase through the scanning electron microscope.

The invention relates to a high-selectivity quick fluorine ion analyzing colorimetric fluorescent probe. Specifically, the colorimetric fluorescent probe is a naphthalimide compound which can serve as the fluorine ion colorimetric fluorescent probe for fluorine ion detection. The probe has at least one of advantages including high selectivity in fluorine ion recognition, quickness in response to fluorine ions, high sensitivity in fluorine ion analysis, property stability, availability for long-term storage and high antijamming capability.

The invention relates to a fluorescent probe for detecting histidine, a precursor thereof and preparation methods of the fluorescent probe and the precursor. The fluorescent probe which can be used for detecting histidine is obtained by preparing precursors of two fluorescent probes in a way of taking naphthalimide or 7-hydroxy coumarin as a fluorophore and complexing with a metal respectively. After histidine is combined, the fluorescent light of a fluorescent probe Ia at a position of 476 nanometers is enhanced slightly, and the fluorescent light of the fluorescent probe Ia at a position of 537 nanometers is enhanced remarkably; and the fluorescent light of a fluorescent probe IIb at a position of 454 nanometers is enhanced remarkably. Moreover, the fluorescent probe provided by the invention has the advantages that: (1) the fluorescent probe has high water solubility, and can be suitable for detecting histidine of a biological system; (2) the fluorescent probe has high stability, can be stored for a long time, and is suitable for various neutral, weakly-acid and weakly-alkaline environment systems, and the like; and (3) the fluorescent probe has the advantages of very high sensitivity, interference resistance (very small interference with other amino acid actions), and the like.

The invention relates to a method for highly selectively detecting the concentration of hydrazine. Particularly, a 4-bromobutyratetricyanostyrene compound is used as a long-wavelength hydrazine colorimetric fluorescent probe and is used for determining the content of the hydrazine in a sample. The probe not only can be used for highly selectively identifying the hydrazine, but also can be used for carrying out sensitive quantitative and qualitative analysis on the hydrazine by using a fluorescent method at a long-wavelength part. The probe can be used for rapidly responding the hydrazine and can realize the instant detection of the hydrazine; and the quantitative and qualitative analysis on the hydrazine can be observed by only naked eyes. The probe has stable characters and can be stored for a long period. Furthermore, the probe also has a relatively strong anti-interference capability.