245results about How to "Long-term storage and use" patented technology

The invention discloses a fluorescent probe for detecting a biologic thiol and a preparation method and a usage method thereof. The fluorescent probe for detecting the biologic thiol consists of two parts: namely, a 2, 4-bi-nitrobenzene sulfonyl group which is a recognizing group and a boron difluoride-dipyrryl methane (BODIPY) derivative which is an information reporting functional group. The molecule of the probe can simply and quickly enter a living cell, generates a specificity reaction with the thiol in the cell, and causes to obviously enhance fluorescence intensity, so that the fluorescent probe further can be used for the fluorescence detection and the imaging of the active thiol in the living cell. The fluorescent probe has good stability, can be stored and used for a long time, is applicable to various environments in which the living cell grows, and has higher detection sensitivity for the thiol, strong anti-interference capability, excellent selectivity and no action on other common biologic interfering molecules. The fluorescent probe can simply enter the living cell and a living tissue; the single recognition of the thiol in a biologic system can be achieved effectively; and therefore, the fluorescent probe can be used for the fluorescence imaging of the living cell.

The invention discloses a preparation method of an adsorbent for removing phosphorus and heavy metal anions in water as well as an application method, and belongs to the fields of adsorbent technology and environmental pollution remediation. The adsorbent is magnetic water hyacinth biochar, and is prepared by the following steps: water hyacinth is dried and pulverized into powder; a proper amount of the water hyacinth powder and a mixed solution containing Fe2+ and Fe3+ are mixed with continuous stirring, and sodium hydroxide is added dropwisely till the pH value is about 10; after filtering, anaerobic pyrolysis is carried out at 250 DEG C or 450 DEG C, and washing, pumping filtration and drying are carried out in order to obtain the magnetic water hyacinth biochar. The application method is as follows: the biochar is added into waste water containing phosphor, arsenic, antimony and chromium, the initial concentration is 0.05-0.5mM, the dosage is 5g / l, a reaction is carried out for 24 hours at 25 DEG C, and the oscillation rate is 150r / min. The adsorbent is advantageous in that the adsorbent has a large capacity for adsorbing phosphor and heavy metal anions in water, the adsorbent is not easy to agglomerate, and after adsorption, the adsorbent is easy to separate and recycle.

The present invention relates to a new-type singlet oxygen europium fluorescent probe and its application. It is a coordination compound formed by using trivalent europium ion Eu3+ and 2, 2í»:6í»2íŒ-ditripyridine skeleton structure ligand containing functional group. Said invention also provides the structure formula of the described ligand. Said coordination compound can be used for making fluorescent determination of singlet oxygen.

The invention relates to a novel rare-earth complex ratiometric fluorescent probe which can be used for measuring peroxynitrite in a biosystem. The probe is complexes formed by two trivalent rare-earth ions Eu<3+> and Tb<3+> and an organic ligand [4'-(2,4-dimethoxyphenyl)-2,2':6'2''-terpyridine-6,6''-dimethylamino]tetraacethyl (DTTA for short), wherein the structural formula of the ligand is disclosed in the specification. The probe can specifically act with the peroxynitrite, so that the two rare-earth complexes are changed at the ratio (F612 / F541) of the fluorescence intensity of 612nm and 541nm, thereby implementing the selective fluorescent measurement of the peroxynitrite in a biosystem.

The invention relates to a novel water-soluble sulfydryl fluorescent probe which is a 2-(2'-hydroxyphenyl) benzimidazole (HPBI) compound modified by quaternary ammonium salt. The compound has the structure of 2-[5-(trimethyl quaternary ammonium salt)-acetamido-2'-hydroxyphenyl) benzimidazole. The fluorescent probe can be used for detecting a biological sulfhydryl compound in aqueous solution and living tumor cell, i.e. the novel water-soluble sulfydryl fluorescent probe is selectively coordinated with cupric ions and an obtained coordination compound can enter the living cell to be reacted with the sulfhydryl compound, so that a fluorescent compound is changed into ionic molecules again and fluorescent light is recovered. The novel water-soluble sulfydryl fluorescent probe has the advantages that the novel water-soluble sulfydryl fluorescent probe has excellent water solubility and biocompatibility and high sulfhydryl measurement sensitivity; before and after GSH (glutathione) reaction, the fluorescent light is rapidly changed and is stable; the novel water-soluble sulfydryl fluorescent probe can be stored and used for a long time; and according to the fluorescent probe, a good research method is provided for researching a biological signal channel related to the biological sulfydryl and the significance and diagnosis of the sulfydryl in the in vivo process of diseases or organisms.

The invention provides a fluorescent probe for detecting biological hydrogen sulfide, of which the fluorescence emission wavelength is greater than 500nm. The fluorescent probe has high stability, and thus, can be stored and used for a long time. The fluorescent probe can effectively avoid interference from the biomacromolecule background fluorescence. The fluorescent probe does not have fluorescence itself, has fluorescence only after reaction with H2S, and has high signal to noise ratio for detection and favorable sensitivity. The fluorescent probe has excellent selectivity, and can specifically detect H2S in a complex biological sample. The fluorescent probe has favorable biomembrane permeability, and thus, can be used for specific detection of biological hydrogen sulfide in living cells. The structural formula of the fluorescent probe is disclosed in the specification.

The invention belongs to the technical field of biological detection, which particularly relates to a fluorescent probe which can be applied in measuring active mercapto image in living cells and the application. The fluorescent probe is 2-(2-hydroxyphenyl) hydroxyphenyl compound which is decorated by maleimide or maleimide acid, the structure of the compound is N-{3-(2- benzimidazolyl)-4-hydroxyphenyl}maleimide and N-{3-(2- benzimidazolyl)-4-hydroxyphenyl}malemide acid. The compound can simply and rapidly get into living cells, which has a reaction with free active mercapto group in cells, and fluorescence intensity of the compound is led to be obviously enhancing, thereby the fluorescent probe can be used in fluorescent measurement or fluorescent image of fluactive mercapto group (such as glutathione and the like) in living cells.

The invention relates to a quick high-selectivity hydrogen sulfide colorimetric probe. Specifically, the probe provided by the invention is a tricyanovinyl nitrogen phenyl compound and can be used as a hydrogen sulfide colorimetric probe for detecting hydrogen sulfide. The probe can realize at least one of the following technical effects: identification of hydrogen sulfide / sodium sulfide with high selectivity; qualitative and quantitative analysis of hydrogen sulfide / sodium sulfide in a naked eye observation mode; quick response to hydrogen sulfide; immediate detection on hydrogen sulfide; stable property and long-term storage; good water solubility favorable for detecting hydrogen sulfide under physiological level conditions; and relatively strong anti-jamming capability.

The invention provides a type of fluorescent probes for detecting formaldehyde. The probes have the structures shown as formulas I to IV. Benzene ring correspondingly substituted coumarin is firstly subjected to backflow reaction with Lawson reagents in toluol; an obtained product continuously performs backflow reaction with hydrazine hydrate in ethanol. The fluorescent probes per se provided by the invention do not have fluorescence in the water solution, but can take a specific fast reaction with formaldehyde to produce products with strong fluorescence, so that the specific sensitive detection and quantitative analysis of formaldehyde is realized. The stability is high; the long-time storage use can be realized; the detection signal-to-noise ratio is high; the sensitivity is high; the excellent selectivity is realized; the specific formaldehyde detection in complicated biological samples can be realized; good biomembrane permeability is realized, so that the fluorescent probes can be used for formaldehyde detection in living cells; the detection reaction is fast; the detection result can be given within 1 minutes for formaldehyde in the water solution; during the formaldehyde detection, the formaldehyde concentration in the environment cannot be influenced. The general molecular formulas of the probes provided by the invention are shown as the accompanying drawing.

The invention discloses a malononitrile derivative near-infrared hydrogen sulfide fluorescent probe as well as a preparation method and application thereof. The probe has a structure represented by aformula I shown in the description. The preparation method comprises the following steps: firstly, mixing isophorone, malononitrile and piperidine, performing heating reflux under the protection of aninert gas, performing purification, mixing the obtained purified product, p-hydroxybenzaldehyde and piperidine, performing heating reflux under the protection of an inert gas, performing purification, mixing the obtained purified product, urotropine and trifluoroacetic acid, performing heating reflux under the protection of an inert gas, performing purification, finally mixing the obtained purified product, 2,4-dinitrobenzenesulfonyl chloride and triethylamine, performing a reaction under stirring at room temperature, and performing purification to obtain the fluorescent probe. The fluorescent probe provided by the invention has large Stokes shift (229 nm), a longer fluorescence emission wavelength (653 nm) and high sensitivity, can be used for specific detection of biological hydrogen sulfide, and has good biofilm permeability and low cytotoxicity; and the fluorescent probe has a simple synthetic route, a high yield and large practical values.

The invention relates to a rapid high-selectivity hypochloric acid fluorescence probe and an application thereof. The probe is 4-hydroquinone based naphthalimide compounds, and the compounds can be used to detect hypochloric acid as a hypochloric acid fluorescence probe. The probe has at least one of the following technical effects: highly-selectively identification of hypochloric acid / hypochlorite ions; rapid response to hypochloric acid / hypochlorite ions; instant detection of hypochloric acid / hypochlorite ions; stable properties and long-term preservation and use; facilitation of detection of hypochloric acid under physiologic level conditions; and strong anti-interference ability.

The invention relates to a rapid high-selectivity fluorine ion colorimetric probe and a preparation method therefor. The probe not only can highly selectively recognize fluorine ions, but also can quantitatively and qualitatively analyze the fluorine ions by way of observation in naked eyes. The probe can be used for quickly responding to the fluorine ions, so that the fluorine ions can be immediately detected. The probe is stable in property, and can be stored and used for a long time. In addition, the probe further can be used for quantitatively analyzing the fluorine ions by virtue of absorption spectroscopy and has relatively strong anti-interference capacity. The probe is synthesized by a room temperature method, requires only one step reaction, and is simple to synthesize.

The present invention relates to a fast and highly selective mercury ion fluorescent probe. The mercury ion fluorescent probe is a sulfocarbonate compound, and the structural formula is as shown in the specification. The mercury ion fluorescent probe can achieve at least one of the following technical effects: highly selective identification of mercury ions; rapid response to the mercury ions; large Stokes shift; stable nature, long term preservation and use; and strong anti-interference ability.

The invention relates to a method for preparing a hydrogen sulphide fast and highly selectively colorimetric probe and particularly relates to a method for preparing a tri-cyano vinyl nitrogen phenyl compound as a hydrogen sulphide fast and highly selectively colorimetric probe and the application of the colorimetric probe. The probe can be used for identifying the hydrogen sulphide highly selectively and analyzing the hydrogen sulphide qualitatively and quantitatively through the observation of naked eyes. The probe is synthesized easily by adopting the heating method through one-step reaction.

The invention relates to the application of a fast and high-selective hydrogen sulphide colorimetric probe and particularly relates to the application of a tri-cyano vinyl nitrogen phenyl compound as a hydrogen sulfide colorimetric probe. The probe can be used for identifying the hydrogen sulphide highly selectively, analyzing the hydrogen sulphide qualitatively and quantitatively by adopting the absorption spectroscopy through the observation of naked eyes and detecting the hydrogen sulphide under the physiological condition immediately. The probe can respond to the hydrogen sulphide fast, has stable nature, good water solubility and strong anti-interference capacity and can be stored and used for a long time. In addition, the probe is synthesized easily by adopting the heating method through one-step reaction.

The invention relates to an anti-scaling agent and a preparation method thereof. The anti-scaling agent comprises the following components by weight percent: 15.0%-20.0% of a barium sulfate anti-scaling agent, 0.10%-0.30% of a wetting inversion agent, 0.10%-0.30% of a surface active agent, 10.0%-15.0% of a mutual solvent, 10.0%-15.0% of a chelating agent and the balance of water. After the anti-scaling agent provided by the invention is injected, the deep part of the ground layer can be cleaned; the back flow is not required; the construction process is simple. The anti-scaling agent provided by the invention has the advantages of wide raw material source, low cost, stable product performance, long service life and long-term storage and use.

The invention discloses a fluorescent probe for detecting mercapto compounds as well as the preparation method and the using method of the fluorescent probe. The prior art difficultly meets the requirements for biological detection and fluorescent imaging. The fluorescent probe consists of two parts, wherein 2,4-dinitrobenzol sulfonyl is an identification group, and dansyl chloride derivative is an information report functional group; and when mercapto exists in a system, the mercapto can perform specific reaction with 2,4-dinitrobenzol sulfonyl, so that the fluorescence of the information report functional group is changed, and the specific detection of the mercapto group is realized. The probe is simple and convenient in molecular synthesis and good in stability, can be stored and used for a long term, has high mercapto detection sensitivity and excellent selectivity, does not act with other common interfering molecules, has long fluorescence emission wavelength (more than 500nm), can effectively avoid the interference from background fluorescence of which biological macromolecules are smaller than 500nm, and can be applied to detection of biological systems.

The invention aims to provide an ammonia adsorbent and a preparation method thereof. The technical scheme adopted by the invention is as follows: furnace slag of coal is smashed, a certain amount of furnace slag powder is taken, a phosphoric acid solution with the concentration of 10-50% is added, impregnating is carried out at the room temperature for 3-24h, and then drying is carried out; after drying, solid matters are heated from the room temperature to the preset temperature (300-700 DEG C), the temperature is kept for 2h, then the heated solid matters are cooled to the room temperature, and the final product is taken out, and is washed with distilled water to be neutral, dried, and bottled for standby, so that the ammonia adsorbent can be obtained.

The invention provides a fluorescence probe ZY9 for visually detecting hydrazine based on benzothiazole as well as a preparation method and use of the fluorescence probe ZY9. The fluorescence probe ZY9 can be prepared through the reaction of 2-(4-aminophenyl)benzothiazole and phthalic anhydride in a certain proportion and is easy to synthesize and purify, relatively high in yield and low in cost;the hydrazine fluorescence probe is a fluorescence enhanced fluorescence probe, has relatively selectivity and sensitivity to hydrazine, is strong in anti-jamming capability and high in response speedand can be used for visually detecting hydrazine through the significant change of the fluorescence color; the probe is good in stability and can be stored for a long time for use; besides, the probeis suitable for detecting the content of hydrazine and realizing the fluorescence imaging of hydrazine in a living cell level, and hydrazine in a water solution with a certain concentration can be detected by virtue of probe test paper. A hydrazine detection method provided by the invention has the advantages of easiness and convenience in operation, easiness in popularization and the like and has wide application prospects in the fields of environment and biological science.

The invention discloses an aptamer capable of specifically identifying vibrio parahaemolyticus. The nucleotide sequence of the aptamer is shown as SEQ. ID. No.1. Vibrio parahaemolyticus is taken as atarget; the optimized aptamer sequence capable of inclusively and specifically identifying the vibrio parahaemolyticus by combining tailoring, directed mutation, LNA replacement and other means on thebasis of an original sequence obtained by SELEX screening; and the optimal aptamer is used for the separation and enrichment of the vibrio parahaemolyticus in a sample. Highly specific detection identification components and application examples which are good in stability, high in affinity, easy in preparation and easy in modification and marking can be provided for the detection of the vibrio parahaemolyticus.

The invention relates to a high-sensitivity high-selectivity fluorescence probe capable of real-time responding hypochlorous acid and application of the high-sensitivity high-selectivity fluorescence probe. The fluorescence probe is a kind of 2-benzothiazoline phenol compounds specifically and can be used as the fluorescence probe to be used for detection of hypochlorous acid / hypochlorite. By utilizing the fluorescence probe, at least one of the technical effects can be realized, for example, hypochlorous acid / hypochlorite can be identified with high sensitivity and high selectivity, response to hypochlorous acid / hypochlorite can be realized instantly, real-time detection to hypochlorous acid / hypochlorite can be realized, the fluorescence probe is stable in character and can be stored and used for long term, detection to hypochlorous acid / hypochlorite is easy under the condition of physiological level, and the fluorescence probe has strong antijamming capability.

The invention provides a barium and strontium scale removing agent and a preparation method thereof. The scale removing agent is prepared from the following raw materials in percentage by weight: 10-15% of hydrochloric acid, 2-5% of hydrofluoric acid, 2-5% of chelating agent, 2-5% of scale inhibitor, 0.5%-1% of corrosion inhibitor, 0.5%-1% of surfactant, 0.1-0.3% of synergist, 0.2%-0.4% of modifier and the balance water. The scale removing agent raw materials are wide in resource and low in price, the prepared scale removing agent is stable in scale removing performance, good in temperature resistance, long in service life, capable of being stored and used for a long term, and high in scale dissolution capability, a novel method is provided for the aspect of oil field acid liquor insolublescale treatment, and the barium and strontium scale removing agent has good application prospects in the aspect of water well decompression and augmented injection.

The invention belongs to the technical field of biological detection and relates to a novel method for detecting the activity of hydrogen sulfide synthetase by using a hydrogen sulfide fluorescence probe as well as an application approach of the method. The method is suitable for detection of the activity of hydrogen sulfide synthetase such as CBS, CSE and CL, and comprises the following steps: shearing a tissue sample into small blocks, grinding, performing cell lysis in a tissue cell lysis solution, performing protein quantification on a lysis product, as the drawings, adding a reaction system (comprising the tissue cell lysis product, L-cysteine and 5-pyridoxal phosphate) into a reaction pool on an outer ring of a reaction bottle, and adding an absorption system (comprising operating fluid of the H2S fluorescence probe containing a reaction adjuvant cetrimonium bromide) into an absorption pool on an inner ring of the reaction bottle, wherein H2S gas molecules generated in the reaction system are combined with the fluorescence probe in the absorption system, the system can be used for fluorescence assay and fluorescence imaging, the H2S content is quantitatively detected, and the activity of the H2S synthetase is intuitively reflected. The invention can provide a good method for research of H2S-related signal paths and significance and diagnosis of H2S in disease processes.

The invention relates to a fast high-selectivity super-sensitive nickel ion colorimetric fluorescent probe and a preparation method thereof. Specifically, the probe provided by the invention is an allyl carbonic ester compound and can be used as a nickel ion colorimetric fluorescent probe for detecting nickel ions. The probe can realize at least one of the following technical effects: high selectively recognizing the nickel ion, quickly responding to the nickel ion, high sensitively analyzing the nickel ion, realizing the naked eye colorimetric analysis for the nickel ion and having stable properties, long-term storage and use and higher anti-jamming capability.

The invention discloses an etchant for observation and energy spectrum analysis of the sigma phase through a scanning electron microscope. The etchant is formed through mixing the following raw materials in percentage by mass: 1-12% of copper(II) sulfate pentahydrate, 8-21% of sodium chloride, 18-41% of phosphoric acid, 0.1-7.5% of absolute ethyl alcohol and 19-72% of distilled water; during the etching process of austenite heat-resistance stainless steel, the etchant composed of copper(II) sulfate pentahydrate, sodium chloride, phosphoric acid, absolute ethyl alcohol and distilled water can effectively restrain an etch pit formed through excessive corrosion, dissolving and falling; the scanning electron microscope can observe that the morphology of the sigma phase in the austenite heat-resistance stainless steel is complete and clear, the situation that an energy spectrometer can be adopted to carry out precise composition detection and analysis on the sigma phase is ensured, and a significance is played in analyzing and determining the failure cause of the austenite heat-resistance stainless steel material; and the etchant is simple in composition, stable in etching effect, and high in reproducibility, can be stored and used for a long time, and is an ideal etchant for observation and energy spectrum analysis of the sigma phase through the scanning electron microscope.

The invention discloses a paraffin slicing method for corydalis saxicola blades. The method comprises the following steps: fixing a sample, dehydrating, transparently processing, waxing, embedding, slicing and unfolding slices, de-waxing, dyeing and sealing slices. The dyeing step further comprises the following steps: before transparently processing, putting the dehydrated corydalis saxicola blades into a safranine solution in volume concentration of 0.8-1.2%, and dip-dyeing for 8-12h; rehydrating a slide carrying the corydalis saxicola blades in the dyeing step; after rehydrating, putting into an alcoholic solution for separating colors; adding acetic acid at the volume ratio of 1 / (800-1000) into the alcoholic solution used for separating colors; and finally, putting into a fast green solution in volume concentration of 0.8-1.2%, and dyeing for 5-15 seconds. The corydalis saxicola blades prepared according to the invention are clear in outline tissues and are suitable for identification of medicinal materials.

The invention relates to a high-selectivity quick fluorine ion analyzing colorimetric fluorescent probe. Specifically, the colorimetric fluorescent probe is a naphthalimide compound which can serve as the fluorine ion colorimetric fluorescent probe for fluorine ion detection. The probe has at least one of advantages including high selectivity in fluorine ion recognition, quickness in response to fluorine ions, high sensitivity in fluorine ion analysis, property stability, availability for long-term storage and high antijamming capability.

The invention belongs to the technical field of fluorescent probe preparation, and particularly relates to a coumarin fluorescent probe and a preparation method thereof. The invention uses 7-hydroxy-4-methyl coumarin and acyl chloride thiophene as raw materials to react under the catalysis of triethylamine to obtain a fluorescent probe compound. Under the excitation of ultraviolet light, the probecompound does not have fluorescence, when it reacts with hydrazine hydrate, the lipid bond is broken, and the probe emits blue fluorescence, thus realizing the detection of hydrazine hydrate. The probe has strong selectivity and high sensitivity to hydrazine hydrate, can realize response to hydrazine hydrate in a complex environment, is an excellent ''turn-on'' type fluorescent probe, and has good application prospect.

The invention relates to a fluorescent probe for detecting histidine, a precursor thereof and preparation methods of the fluorescent probe and the precursor. The fluorescent probe which can be used for detecting histidine is obtained by preparing precursors of two fluorescent probes in a way of taking naphthalimide or 7-hydroxy coumarin as a fluorophore and complexing with a metal respectively. After histidine is combined, the fluorescent light of a fluorescent probe Ia at a position of 476 nanometers is enhanced slightly, and the fluorescent light of the fluorescent probe Ia at a position of 537 nanometers is enhanced remarkably; and the fluorescent light of a fluorescent probe IIb at a position of 454 nanometers is enhanced remarkably. Moreover, the fluorescent probe provided by the invention has the advantages that: (1) the fluorescent probe has high water solubility, and can be suitable for detecting histidine of a biological system; (2) the fluorescent probe has high stability, can be stored for a long time, and is suitable for various neutral, weakly-acid and weakly-alkaline environment systems, and the like; and (3) the fluorescent probe has the advantages of very high sensitivity, interference resistance (very small interference with other amino acid actions), and the like.

The invention relates to a method for highly selectively detecting the concentration of hydrazine. Particularly, a 4-bromobutyratetricyanostyrene compound is used as a long-wavelength hydrazine colorimetric fluorescent probe and is used for determining the content of the hydrazine in a sample. The probe not only can be used for highly selectively identifying the hydrazine, but also can be used for carrying out sensitive quantitative and qualitative analysis on the hydrazine by using a fluorescent method at a long-wavelength part. The probe can be used for rapidly responding the hydrazine and can realize the instant detection of the hydrazine; and the quantitative and qualitative analysis on the hydrazine can be observed by only naked eyes. The probe has stable characters and can be stored for a long period. Furthermore, the probe also has a relatively strong anti-interference capability.