Method for highly selectively detecting concentration of hydrazine

A technology for concentration and sample detection, applied in chemical instruments and methods, measuring devices, instruments, etc., can solve the problems of complex synthesis, poor selectivity, low sensitivity, etc., and achieve the effect of simple synthesis and good stability

Active Publication Date: 2016-03-30
晋江尚京富本环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recently, fluorescent probes have been rapidly developed due to their advantages of high sensitivity and convenient operation, but there are still some deficiencies in the reported hydrazine fluorescent probes, such as poor selectivity, low sensitivity, slow response, complex synthesis, and excitation and emission. relatively short wavelength

Method used

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  • Method for highly selectively detecting concentration of hydrazine
  • Method for highly selectively detecting concentration of hydrazine
  • Method for highly selectively detecting concentration of hydrazine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024]

[0025] (Scheme 1) 4-Hydroxystyrenetricyanofuran (303mg, 1.0mmol), 4-bromobutyric acid (166mg, 1.0mmol), 4-dimethylaminopyridine (122mg, 1.0mmol) and dicyclohexyl carbon Diimine (412 mg, 2.0 mmol) was dissolved in 20 mL of dry dichloromethane, reacted at 50 °C for 4 h, evaporated under reduced pressure to obtain a crude product, and then separated by column chromatography using dichloromethane to obtain 140 mg of a light yellow pure product. The yield was 31%.

[0026] (Scheme 2) 4-Hydroxystyrenetricyanofuran (303mg, 1.0mmol), 4-bromobutyric acid (166mg, 1.0mmol), 4-dimethylaminopyridine (122mg, 1.0mmol) and dicyclohexyl carbon Diimine (412 mg, 2.0 mmol) was dissolved in 20 mL of dry dichloromethane, reacted at 50 °C for 10 h, evaporated under reduced pressure to obtain a crude product, and then separated by column chromatography using dichloromethane to obtain 293 mg of a light yellow pure product. The yield was 65%.

[0027] (Scheme 3) 4-Hydroxystyrene tricyanof...

Embodiment 2

[0031] The inventor of the present invention has carried out following test: (a) the impact of different concentrations of hydrazine (0~500 μ M) on the absorption spectrum of probe (20 μ M), insert figure is the ratio of the absorbance at 595nm place and the absorbance at 410nm place and the concentration of hydrazine added linear relationship between them. (b) Effects of different concentrations of hydrazine (0-500 μM) on the fluorescence spectrum of the probe (20 μM); the insert graph is the linear relationship between the fluorescence intensity at 610 nm and the concentration of added hydrazine. The above determinations were made in a system containing ethanol and water (5:5, V / V) containing 5mM phosphate buffered saline (PBS) with a pH value of 7.4, and all spectral tests were performed at 25°C after adding hydrazine for 20 minutes. of. See results figure 1 .

[0032] From figure 1 a It can be seen that with the increase of the concentration of hydrazine in the probe s...

Embodiment 3

[0034] Depend on figure 2 It can be clearly seen that the color of the probe solution (40 μM) gradually changes from yellow to blue in the presence of different concentrations of hydrazine (from left to right: 0, 0.2, 0.4, 0.6, 0.8, 1.0 mM). Therefore, the probe of the present invention can complete the qualitative and quantitative analysis and detection of hydrazine in the sample only by "naked eye" observation.

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Abstract

The invention relates to a method for highly selectively detecting the concentration of hydrazine. Particularly, a 4-bromobutyratetricyanostyrene compound is used as a long-wavelength hydrazine colorimetric fluorescent probe and is used for determining the content of the hydrazine in a sample. The probe not only can be used for highly selectively identifying the hydrazine, but also can be used for carrying out sensitive quantitative and qualitative analysis on the hydrazine by using a fluorescent method at a long-wavelength part. The probe can be used for rapidly responding the hydrazine and can realize the instant detection of the hydrazine; and the quantitative and qualitative analysis on the hydrazine can be observed by only naked eyes. The probe has stable characters and can be stored for a long period. Furthermore, the probe also has a relatively strong anti-interference capability.

Description

technical field [0001] The invention relates to a method for detecting the concentration of hydrazine with high selectivity. Specifically, the present invention relates to a method for using 4-bromobutyrate tricyanofuran styrene compounds as a highly selective long-wavelength colorimetric fluorescent probe for hydrazine and an application for highly selective recognition of hydrazine. Background technique [0002] Hydrazine (NH 2 NH 2 ) is widely used in industrial fields such as organic synthesis because of its strong reducibility. In addition, hydrazine has also been applied in the field of medicine as an antibacterial drug. However, studies have shown that hydrazine is highly toxic and can increase the incidence of lung cancer, rhinitis cancer, and liver cancer. Now hydrazine has been listed as a potential human carcinogen by the US Environmental Protection Agency and the World Health Organization. As we all know, hydrazine has strong volatility and is easy to cause ...

Claims

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Application Information

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IPC IPC(8): G01N21/64C09K11/06C07D307/68
CPCC07D307/68C09K11/06C09K2211/1088G01N21/643
Inventor 叶龙武张艳
Owner 晋江尚京富本环保科技有限公司
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