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Azo fluorescent probe for detecting biological hydrogen sulfide and application thereof

A fluorescent probe and azo-based technology, applied in the field of fluorescent probes for the detection of biological hydrogen sulfide, can solve the problems of large detection errors and achieve the effects of avoiding interference, high detection signal-to-noise ratio, and excellent selectivity

Inactive Publication Date: 2014-09-10
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the detection errors of these two methods are relatively large, and they can only measure H in plasma or tissue homogenate. 2 S, cannot be used to measure H in cells or even living tissues 2 S

Method used

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  • Azo fluorescent probe for detecting biological hydrogen sulfide and application thereof
  • Azo fluorescent probe for detecting biological hydrogen sulfide and application thereof
  • Azo fluorescent probe for detecting biological hydrogen sulfide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 : The probe molecule shown in the following formula II with H 2 Fluorescence changes before and after S reaction

[0016]

[0017] Dissolve the probe molecule with a small amount of ethanol, and add PBS buffer or NaHS in PBS solution respectively, so that the final concentration of the probe molecule is 20 μM, and the final concentration of NaHS is 500 μM. After reacting for 1.0 hour, measure on the fluorescence spectrometer, and then determine the probe molecule and H 2 After the S reaction, the fluorescence intensity was significantly enhanced, and the results can be found in figure 1 .

Embodiment 2

[0018] Example 2 : the probe molecule shown in the following formula III with H 2 Fluorescence changes before and after S reaction

[0019]

[0020] Dissolve the probe molecule with a small amount of ethanol, and add PBS buffer or NaHS in PBS solution respectively, so that the final concentration of the probe molecule is 20 μM, and the final concentration of NaHS is 500 μM. After reacting for 1.0 hour, measure on the fluorescence spectrometer, and then determine the probe molecule and H 2 After the S reaction, the fluorescence intensity was significantly enhanced, and the results can be found in figure 2 .

Embodiment 3

[0021] Example 3 : probe molecule II to H 2 S selectivity

[0022] Dissolve the probe molecules with a small amount of ethanol, and then use PBS buffer to form a solution. The samples to be tested dissolved in PBS buffer were respectively added, so that the final concentration of the probe molecule was 20 μM, and the concentration of the sample to be tested was 500 μM. After reacting for 1.0 hour, measure on the fluorescence spectrometer, and then determine the probe molecule pair H 2 S selectivity. See results image 3 , the probe molecule pair H 2 S is highly selective.

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Abstract

The invention provides a fluorescent probe for detecting biological hydrogen sulfide, of which the fluorescence emission wavelength is greater than 500nm. The fluorescent probe has high stability, and thus, can be stored and used for a long time. The fluorescent probe can effectively avoid interference from the biomacromolecule background fluorescence. The fluorescent probe does not have fluorescence itself, has fluorescence only after reaction with H2S, and has high signal to noise ratio for detection and favorable sensitivity. The fluorescent probe has excellent selectivity, and can specifically detect H2S in a complex biological sample. The fluorescent probe has favorable biomembrane permeability, and thus, can be used for specific detection of biological hydrogen sulfide in living cells. The structural formula of the fluorescent probe is disclosed in the specification.

Description

technical field [0001] The invention belongs to the field of biological detection, and relates to a fluorescent probe for detecting biological hydrogen sulfide and an application thereof. Background technique [0002] Hydrogen sulfide (H 2 S), a colorless, flammable gas with the smell of rotten eggs, has long been considered a poisonous substance. However, recent studies have shown that mammals can 2 Under the catalysis of S synthetase, a small amount of H is synthesized from homocysteine ​​or cysteine. 2 S, and use it as a signaling molecule to regulate physiological processes such as vasodilation, inflammatory response, and glucose metabolism. h 2 S is the third gas messenger discovered after NO and CO. It can dilate blood vessels by opening ATP-sensitive potassium ion channels in vascular smooth muscle, thereby achieving the effect of lowering blood pressure. h 2 S also has cardiovascular protective effects such as promoting angiogenesis, vascular remodeling, anti-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06G01N21/64C07D493/10
Inventor 李新胡永洲程娟
Owner ZHEJIANG UNIV
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