195results about How to "Short screening cycle" patented technology

The present invention discloses a method which adopts the aptamer rapidly to detect the malachite green in water products. The sample to be detected, the malachite green aptamer and the report sequence with detection markers are mixed; after the report sequence and the malachite green are competitively combined with the malachite green aptamer, the markers on the report sequence is detected, and the content of the malachite green in the sample to be detected is calculated. The kit and the fast detecting test paper, which are prepared according to the method, can better satisfy the current urgent needs for detecting the malachite green residue in the water products, can be used in the detection of the customs and the food sanitation department, and can be easily promoted and applied in a large scale.

The embodiment of the invention provides an image screening method and device. The method comprises the following steps: obtaining an image frame sequence to be screened, wherein, each image frame inthe image frame sequence to be screened comprises a first target object; obtaining an image frame sequence to be screened. Determining target attribute data of a preset attribute of a first target object in each frame of an image to be screened by using a preset image feature evaluation model; According to the target attribute data of the first target object, determining the target confidence level corresponding to the image to be screened in each frame for the first target object in each image to be screened. According to the target confidence level corresponding to the image to be screened in each frame, the target image whose target confidence level reaches the preset recognition condition is screened out from the image frame sequence to be screened. The embodiment of the invention realizes automatic screening of images, shortens screening period and improves screening efficiency.

The invention provides fowlpox virus vector shuttle plasmid pTGP3 which comprises recombinant arms TKL and TKR, a bidirectional promoter PE/L, a fluorescent protein expression cassette, and a resistant marker gene and replication origin ori; the upstream and the downstream of the bidirectional promoter PE/L are respectively provided with cloning sites MCSL and MCSR; and both ends of the fluorescent protein expression cassette are provided with loxp sequences. The plasmid of the invention has two different screening markers, and the recombinant fowlpox virus prepared with the plasmid can express 1 to 3 types of gene with different meshes in the whole processes of the early and the later periods; the strong composite promoter with expression activity in the early and the later periods is applied so as to realize the all-process high-efficiency expression of a target gene; and the loxp sequences are introduced into both ends of the fluorescent protein expression cassette, so as to knock out the exogenous recombinant fowlpox virus screening markers. The invention lays foundation for the series and the scale application of the recombinant fowlpox virus in vaccine and biological drug research and development fields.

The invention provides a high-flux anti-tumor drug screening cell model based on an STAT3 and NF-kB two-signal channel serving as a target. In the cell model, the specific binding sequence of STAT3 and that of NF-kB are bound to build a reporting system carrier; and the cell model is built by binding competent cells comprising both constitutive STAT3 and constitutive NF-kB. The cell model is used for screening and treating cell cancerization caused by continuous activization of STAT3 and/or NF-kB, and medicines generated by the combination and the mutual promotion of STAT3 and NF-kB, and has a higher efficiency than a single-signal medicine screening system. As the competent cells comprising both constitutive STAT3 and constitutive NF-kB are adopted, the reporting gene in the cell model is in a highly active state, no stimulus is required to be added additionally, the screening process is simplified, the screening cost is reduced, the stability, the high efficiency and the easiness in the use of a system are improved, and the cell model is more suitable for high-flux medicine screening.

The invention relates to a lithium ion battery self-discharging screening method. The lithium ion battery self-discharging screening method comprises the following steps: S1, performing first-time voltage test on to-be-tested batteries according to a first test standard and screening out the to-be-tested batteries meeting the first test standard; S2, ageing the to-be-tested batteries meeting the first test standard by a preset ageing process; S3, performing second-time voltage test on the to-be-tested batteries aged by the preset ageing process according to a second test standard and screeningout the to-be-tested batteries meeting the second test standard; S4, screening the to-be-tested batteries meeting the second test standard and screening out the to-be-tested batteries with self-discharging SOC being 5 to 15 percent; and S5, screening the to-be-tested batteries with the self-discharging SOC being 5 to 15 percent by a K value method and screening out the to-be-tested batteries meeting the K value. By the method, the self-discharging screening cycle can be effectively shortened, the productivity is improved, the cost is further reduced, the efficiency is high and the accuracy degree is high; furthermore, the quality of products can be improved and the customer complaint risk can be reduced; meanwhile, the delivery cycle of the products can be shortened.

The Chinese medicine developing process includes selecting traditional Chinese recipe with determined curative effect, separating the components, optimizing the component combination, determining thescreening model and performing medicine effect test based on Chinese medicinal theory and compound Chinese medicine features, computer aided analysis to recognize effective components, combining modeand medicinal effect, and optimizing new Chinese medicine. The said process can raise the screening efficiency of effective components, shorten screening period and embody compound Chinese medicine features. Altering the combination mode of medical herb pieces into combination of effective components develops modern Chinese medicine preparation with clear matter basis and controllable quality, being safe and effective and coinciding with international standard.

The invention discloses an oligonucleotide library classification and assessment method based on capillary zone electrophoresis, and belongs to the field of creature isolation analysis. The method comprises the following steps of: step one, carrying out capillary zone electrophoresis on an oligonucleotide library, and obtaining a secondary library within an oligonucleotide library electrophoresis time range according to transfer time slicing collection; and step two, respectively mixing each secondary library with a homogeneous target molecule, carrying out capillary zone electrophoresis, and comparing the strong or weak of each secondary library and target molecule interaction, thus obtaining the strongest secondary library of the target molecule combining capacity. The classification and assessment method can be used for realizing the fractionation of complicated constituent oligonucleotide library, and obtaining the strongest secondary library of the target molecule combining capacity; and the strongest secondary library of the combining capacity is utilized as a next CE (capillary electrophoresis)-SELEX(systematic evolution of ligands by exponential enrichment) technical screening library, the screening range is reduced, and the screening period of an adaptation body is shortened.

The invention relates to a method for screening single chain antibodies of Microcystin-LR and verification thereof. The method comprises the following steps: carrying out two rounds of affinity screening on the biotinylated Microcystin-LR in a human source synthetic antibody library by using an avidin labeled magnetic bead and a negative screening method; extracting total plasmid DNA from phage colonies produced in the second round, carrying out enzyme digestion with enzyme Sfi I, recycling gel to obtain single chain antibody genes, connecting the single chain antibody genes with soluble expression carrier pAK100CL which is processed by enzyme digestion in the same way, and electrically transforming the connected carrier into colibacillus XL1-Blue to obtain soluble expression single chain antibodies; and verifying the soluble expression single chain antibodies by using a competitive time-resolved fluorescence immune analytical method. The invention has the advantage of quick, simple and convenient screening, and can well expose the Microcystin-LR three-dimensional structure into the incubation system. The verification on the screening result has the advantages of high detection signal and strong anti-interference capacity against stroma.

The invention relates to a friction wearing part test apparatus for a connecting rod small end bushing of an engine. The apparatus comprises a body (1), a bearing cover (2), a crankshaft (3), a connecting rod (4), a cylinder sleeve (5), a test piston (6), a piston pin (7) and a small end bushing (8). The crankshaft (3) performs rotary motion in a bearing structure composed of the body (1) and the bearing cover (2). The crankshaft (3) and the connecting rod (4) are connected, the connecting rod (4) is connected with the test piston (6) through the piston pin (7), the contact portion of the connecting rod (4) and the piston pin (7) is provided with the small end bushing (8), and the rotary motion of the crankshaft (3) drives the test piston (8) to perform up-and-down motion in the cylinder sleeve (5). The apparatus further comprises a support rod (9), a spring limiting disc (10), a motion disc (11) and a spring set (12). Compared to a whole machine test, the apparatus can reduce the cost for endurance testing of a connecting rod small end bushing.

The invention belongs to the technical field of cell research, and particularly relates to a method for constructing a laryngeal cancer cell line for stably expressing the green fluorescent protein. The method comprises the following steps: (1) designing and synthesizing a primer; (2) digesting the gene sequence of pLenti-puro carrier plasmids with BamHI and XhoI according to the gene sequence andpLenti-puro sequence of the green fluorescent protein, and connecting digested products to obtain a target carrier pLenti-puro-GFP; (3) verifying the carrier pLenti-puro-GFP with endotoxin extraction, digesting the pLenti-puro-GFP plasmids with BamHI and XhoI, and detecting the gene sequence by agarose gel electrophoresis, and sequencing the carrier pLenti-puro-GFP plasmids to detect the sequencesituation of the GFP-LC3; and (4) co-transfecting a 293T cell with the carrier pLenti-puro-GFP plasmids and a lentivirus package carrier, collecting virus supernatant, and transfecting a laryngeal cancer cell Hep-2 with the virus supernatant, infecting for 24 hours, then adding a culture medium containing puromycin, screening the cell, and observing under high content until a stable strain is obtained.

The invention discloses a simple rapid high-throughput screening method of brassica plants with low accumulation of heavy metal cadmium and relates to the technical field of brassica plants breeding and screening of germplasm resource. The method comprises the following steps: (1) seed sterilization; (2) preparation of a seed germination substratum; (3) seed distribution; (4) bud forcing treatment; (5) seed germination and growth; (6) radicle length measurement; (7) evaluation of radicle length inhibition effect in cotyledon stage; and (8) screening of brassica plants with low accumulation of heavy metal cadmium from the tested group. Only by processing the tested seeds of brassica plants with a certain concentration of cadmium solution on the seed germination substratum, brassica plants with low accumulation of heavy metal cadmium can be screened out. The method is simple and labor-saving. It only takes one week in screening. Thus, screening period is greatly shortened, and screening course is accelerated. Brassica plants with low accumulation of heavy metal cadmium can be screened from a large group with high throughput. The method is not dependent on expensive measuring instruments, is low-cost and is easy for large-scale promotion and application.

The invention discloses a method for obtaining high-yield curdlan bacterial strain through high-flux screening. The method comprises the following steps: 1) selecting NTC-mutagenic soil agrobacterium single colony; 2) employing a 96 orifice plate for primary screening; 3) performing secondary screening by a test tube; and 4) swing a bottle for verification. According to the invention, aniline blue is taken as a curdlan color developing substance while mutagenesis primary screening. The method has the advantages of rapidity and accuracy, can produce the curdlan bacterial strain with high yield through high-flux screening, can greatly reduce the cost, and increases the screening efficiency.

The invention relates to Saccharomyces cerevisiae and a preparation method thereof. A preparation method of high-nitrogen fresh yeast is mainly characterized by comprising the following steps of: (1) preparing a strain, i.e., Saccharomyces cerevisiae which is collected in the China General Microbiological Culture Collection Center with the collection number of CGMCC No.5004; (2) preparing a culture medium in a certain mass ratio; (3) pretreating sweet sorghum juice; (4) fermenting; (5) undergoing an adaptive phase at the ventilation quantity of 0.8-1.2 V.V.m and the stirring speed of 300-500 r/min for 4-6 hours, and adding nutritional salts, including 0.2-3 percent of (NH4)2SO4, 0.03-1.5 percent of MgSO4 and 0.2-1.5 percent of KH2PO4; (6) undergoing an exponential growth phase for 10-20 hours, fermenting at the fermenting temperature of 32-35 DEG C, undergoing a decline phase at the ventilation quantity of 0.3-0.5 V.V.m and the stirring speed of 80-150 r/min, stopping feeding liquid glucose, measuring the concentration of residual sugar in fermented cheese liquid, and stopping fermenting if the concentration of residual sugar is less than 0.5 percent; and (7) treating a fermentation liquid. In the method, the sweet sorghum juice is taken as a carbon source in the fermentation liquid. Compared with other carbon source culture mediums such as honey and beet, the sweet sorghum juice has the advantages of simple pretreating process, saving in energy consumption and increase in production efficiency.

The invention relates to a screening method of a cell strain of a GS expression system. The method includes the steps of: pressurizing methionine sulphoximine, selecting a cell pool being 25-35% in cell activity for performing monoclonal screening, and adding the cell pool to a semi-solid culture medium containing 10% of a conditional culture medium to form mono-clone (wherein final cell concentration is 150 cells/ml), and then performing screening and amplified cultivation to obtain a high-expression cell strain through a high-throughput cell screening system, and performing stability evaluation to the screened high-expression cell strain. The method can be used for obtaining the cell strain being more than 90% in stability.

The invention discloses a thermophilic esterase AFEST mutant. An amino acid sequence of the thermophilic esterase AFEST mutant is different from an amino acid sequence SEQ ID NO.2 of wild type thermophilic esterase AFEST in 1-5 amino acid residues; the difference comprises replacement or deficiency or insertion of amino acid and is further shown in L47R or S111T or A166V or D175V. The invention further discloses a gene for coding the thermophilic esterase AFEST mutant, a recombinant vector containing the gene and an engineering bacterium containing the gene. The invention further discloses a screening method of the thermophilic esterase AFEST mutant and application of the thermophilic esterase AFEST mutant in hydrolysis of short-chain carboxylic ester substrates. According to the thermophilic esterase AFEST mutant, the hydrolytic activity on p-nitrophenol butyric ester is increased by 4 times or above, and the heat stability equivalent to that of the wild type thermophilic esterase AFEST is maintained.

The invention discloses a method for high throughput screening of microorganisms for preventing plant soil-borne fungal diseases, and belongs to the technical field of agricultural microorganisms. Themethod includes preparing the diseased oil with a high-concentration wheat root rot pathogenic bacteria culture and the soil, sowing germinated wheat seeds into the soil containing pathogenic bacteria to simulate the natural growth conditions and environment of plants and the pathogenic bacteria in the soil to the maximum extent. The method for screening for a pathogen-plant pathogenesis system of antagonistic microorganisms of the wheat bipolaris sp, the dense planting of the wheat reduces the space occupied by the screening, and the symptoms of the wheat root rot appear early, which reducesthe screening time, so that the high-throughput screening can be achieved. The screening efficiency of the microorganisms with better inhibition of pathogenic bacteria obtained in the early screeningis greatly improved, and a large number of microorganisms that cannot exert control effects in practical applications can be removed, so that the pace of microorganism screening, microorganism pesticide development and microorganism fertilizer research and development can be accelerated.

The invention discloses a breeding method of tomato disease-resisting homozygotes. The method comprises a tissue culture and identification step and a disease-resisting screening step, wherein tissueculture comprises the following steps: preparing a specific culture medium formula based on a conventional culture medium formula by replacing sucrose with trehalose and adding nutrient components oflycium ruthenicum juice filtrate and preparing an explant, carrying out callus induced culture on anther, carrying out callus culture, obtaining a complete plant, treating with colchicine and carryingout ploidy determination to obtain homozygote seedlings; combining a molecular marking technology to carry out disease-resisting molecular marker screening, so as to obtain disease-resisting homozygote plants. According to the method disclosed by the invention, a screening period can be remarkably shortened and the tomato disease-resisting homozygotes are rapidly obtained; moreover, the method issimple to operate and low in cost; after anther culture seedlings are transplanted, the survival rate is high and the stress resistance is strong; the method is easy to popularize, can be directly applied to production and has relatively good economical benefits and social benefits.

The invention discloses a method for screening myocardial ischemia resisting pharmacodynamic material basis of yang exhaustion treating decoction. The method comprises the following steps: 1, determining myocardial ischemia resisting endogenous metabolin of the yang exhaustion treating decoction and measuring the peak area; 2, determining in-vivo migration constituents in the yang exhaustion treating decoction and measuring the peak area; 3, performing correlation analysis on the peak area of the myocardial ischemia resisting endogenous metabolin of the yang exhaustion treating decoction and the peak area of the in-vivo migration constituents in the yang exhaustion treating decoction and screening out myocardial ischemia resisting pharmacodynamic material basis of the yang exhaustion treating decoction. The method disclosed by the invention is based on isoproterenol-induced rat myocardial ischemia model; an ultrahigh performance liquid chromatography-quadrupole rod-flight time mass spectrum non-targeted metabonomic technology and a targeted metabonomic technology based on an ultrahigh performance liquid chromatography-triple quadrupole rod mass spectrum are utilized to screen out 12 kinds of myocardial ischemia resisting pharmacodynamic material basis of the yang exhaustion treating decoction; thus, accuracy of the myocardial ischemia resisting pharmacodynamic material basis ofthe yang exhaustion treating decoction is improved, a screening period is shortened, and screening consumption cost is reduced.

The invention discloses a method for screening root media by using tobacco sterile seedlings. The method comprises the following steps: A, sterilizing tobacco seeds; B, cultivating the sterile seedlings; C, shearing the sterile seedlings; D, allocating a plurality of root media to be screened; and E, screening the optimal root media. The method is simple in process, labor-saving and time-saving, economic and reliable, and can shorten the screening period effectively, simplify the screening program, and save the screening material; and the sterile seedlings materials are obtained by seeds in a culture vessel directly, and enter the stage of root screening directly, which omits the trouble of cultivating adventitious buds as compared with the conventional screening test, so that manpower, material resources and time are saved. The tobacco sterile seedlings are adopted to screen the media, and the tobacco sterile seedlings screening can synchronize with the callus induction, so that the test schedule is speeded up. Besides, the induction effect of the method is consistent with that of a method for screening root media by using adventitious buds.

The invention discloses a DNA bar code used for screening high-quality Tibet brown mushrooms, a primer and application of the DNA bar code used for screening the high-quality Tibet brown mushrooms andthe primer. The DNA bar code used for screening the high-quality Tibet brown mushrooms contains one or more of 17 DNA fragments with nucleotide sequences shown in SEQ ID NO: 1-17 as shown in the description. The amplification primer of the DNA bar code used for screening the high-quality Tibet brown mushrooms contains one or more of 17 pairs of primers with upstream and downstream nucleotide sequences respectively shown in SEQ ID NO: 18-51 as shown in the description. Compared with a traditional breeding method and other existing DNA bar code technologies, the DNA bar code used for screeningthe high-quality Tibet brown mushrooms, the primer and application of the DNA bar code used for screening the high-quality Tibet brown mushrooms and the primer have the advantages of being time-saving, energy-saving, money-saving, accurate and efficient, and play a positive role in genetic breeding of the high-quality Tibet brown mushrooms, and an effective method is provided for identification and protection of germplasm resources at the same time.

The invention provides a Wnt-targeted drug screening cell model, which is constructed by using a Wnt specific binding sequence-containing report system vector and taking a constitutive Wnt-containing active cell as the basis. The model is used for screening drugs treating Wnt constitutive activation caused tumors and immunological diseases. Due to employment of cells with constitutive Wnt activity, the reporter gene in model cells is in a highly activated state with no need to use additional irritant, and the obtained stable cell model can be directly used for compound screening. According to the invention, the screening cost is greatly reduced, and also, the whole selection process is simplified from cell culture, Wnt activation, compound adding, and detection to cell culture, compound adding, and detection. With the model, the screening cycle is shortened, the operation steps are reduced, and the system stability, high efficiency and usability are enhanced. Thus, the cell model is more suitable for high-throughput drug screening.

The invention relates to the field of biomedicine, in particular to a preparation method of snake venom thrombin-like protein. Firstly, the venom tissue of Agkistrodon akistrosus is isolated, and a phage display library is constructed. Using fibrin/fibrinogen as a substrate, three rounds of panning selected, a new thrombin-like gene from Agkistrodon venom was obtained. The present invention also constructs the prokaryotic expression vector pET-32(a)-TLE of the viper venom thrombin gene of Agkistrodon akistrodon, and obtains the recombinant snake venom thrombin with biological activity after induced expression, affinity purification and molecular sieve purification protein. The enzyme can be used to prepare cardiovascular thrombolytic drugs.