4results about How to "Short screening cycle" patented technology

This invention provides a method for screening highly specific nucleic acid aptamers and specific *Lactobacillus helveticus* aptamers, belonging to the field of nucleic acid aptamers. Specifically, the method for screening highly specific nucleic acid aptamers provided by this invention includes the following steps: S1: A second library is obtained after N rounds of forward screening of a first library; S2: A third library is obtained after M rounds of reverse screening of the second library; S3: The third library is obtained after X rounds of forward screening of the third library to obtain selected random single-stranded oligonucleotides, which are then subjected to PCR amplification, purification, cloning, and sequencing; S4: The sequences cloned and sequenced in step S3 are used for affinity and specificity assays to select sequences with high affinity and specificity, thereby obtaining highly specific nucleic acid aptamers. The aptamers provided by this invention have the advantages of high specificity and strong affinity, and the construction method of the aptamers provided by this invention has the advantage of short screening time.

A kind of inorganic solid electrolyte performance modeling method using graph theory and machine learning.The method abstracts atoms in inorganic solid electrolyte material into a crystal network graph composed of nodes and edges, after feature embedding, multi-layer convolution is carried out using crystal graph convolutional neural network, and the local chemical environment feature vector of atom is updated by aggregating neighborhood features;Then, through the global aggregation of the convergence layer, the feature vector representing the entire crystal structure is extracted;Finally, through the mapping of the full connection layer, the multi-dimensional performance indexes such as thermodynamics, mechanics and electrochemistry are output.The present application realizes the prediction of the structure-property relationship between material structure and macroscopic performance by precisely depicting the local environment of atoms, thereby guiding the efficient development of new inorganic solid electrolyte materials.

This invention belongs to the field of active ingredient extraction, specifically relating to a purslane extract screened using bioaffinity ultrafiltration, its preparation method, and its applications. This invention utilizes bioaffinity ultrafiltration to rapidly capture active small molecules that specifically bind to XOD from purslane extract, significantly shortening the screening cycle for natural product XOD inhibitors. Furthermore, the screened compounds have been verified in vitro to possess XOD inhibitory activity. The method exhibits high sensitivity and good reproducibility. This invention is the first to discover and clarify Ophiogonanone A, Cimidahurinine, and Cyanidin-3- O γ-glucoside is a natural XOD inhibitor found in purslane, providing a new natural candidate molecule for drugs that lower uric acid or treat gout.

This invention discloses a DNA barcode, primers, and their applications for screening high-quality Tibetan brown mushrooms. The DNA barcode for screening high-quality Tibetan brown mushrooms comprises one or more of the 17 DNA fragments with nucleotide sequences as shown in SEQ ID NO: 1-17; the DNA barcode amplification primers for screening high-quality Tibetan brown mushrooms comprise one or more pairs of the 17 primer pairs with upstream and downstream nucleotide sequences as shown in SEQ ID NO: 18-51. Compared with traditional breeding methods and other existing DNA barcoding technologies, this invention has the advantages of being time-saving, labor-saving, cost-effective, accurate, and efficient, playing a positive role in the genetic breeding of high-quality Tibetan brown mushrooms, and also providing an effective method for the identification and protection of germplasm resources.