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Test strip for testing chloramphenicol by adopting time-resolved fluorescence lateral flow immunoassay quantitative testing

A technology of time-resolved fluorescence and immunochromatography, applied in measuring devices, analytical materials, biological testing, etc., can solve the problems of low sensitivity, detection influence, small stock displacement, etc., and achieve the effect of high sensitivity

Inactive Publication Date: 2019-05-28
NANJING YITE BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The rapid detection products developed based on colloidal gold labeling technology have problems such as qualitative or semi-quantitative, and large batch-to-batch differences; although the batch-to-batch difference of colored latex microspheres has improved, the sensitivity is still low and can only be qualitative or semi-quantitative ; The sensitivity of immunochromatography based on fluorescein labeling technology has been greatly improved, and quantitative detection can also be performed, but because the sample contains a high fluorescent background signal and the stock shift is small, it will have a greater impact on the detection

Method used

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  • Test strip for testing chloramphenicol by adopting time-resolved fluorescence lateral flow immunoassay quantitative testing
  • Test strip for testing chloramphenicol by adopting time-resolved fluorescence lateral flow immunoassay quantitative testing
  • Test strip for testing chloramphenicol by adopting time-resolved fluorescence lateral flow immunoassay quantitative testing

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Experimental program
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Embodiment Construction

[0035] 1. Preparation of Components of Test Strips

[0036] 1.1 Preparation of quality control microspheres Preparation of quality control microspheres coated with biotinylated γ-globulin (BGG)

[0037] ⑴ Preparation of biotinylated BGG

[0038] with 0.1 M NaCNBH 3 BGG (purchased from Pel-Freez Biological) was prepared into a 10 mg / mL solution, and Biotin-X-X-NHS (N-hydroxysuccinimide modified biotin, manufacturer: SIGMA, Product number: B3295) solution to 16.2 mg / mL, add Biotin-X-X-NHS solution to BGG solution according to the amount of 1 mg BBG protein and 5.4 μl Biotin-X-X-NHS solution, mix well and place overnight at 4°C. Dialysis was used to remove free unreacted biotin, and the dialysate was biotin-labeled protein dialysate buffer (0.1 M Tris, 0.3 M Nacl, 0.005 MEDTA-Na-2H 2 0, pH8.0). After dialysis, the protein concentration was determined by BCA method.

[0039] ⑵ Use the above-mentioned labeled protein to coat the aldehyde-modified fluorescent microspheres

[0...

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Abstract

The invention relates to a preparation method and application of a quick test strip for testing chloramphenicol by adopting time-resolved fluorescence lateral flow immunoassay quantitative testing. The test strip is composed of a Fusion5 membrane, a nitrocellulose membrane and water absorption paper, according to the competition law principle, time-resolved fluorescence microspheres are taken as an immune marker, and accurate and quick quantitative testing can be conducted on a to-be-tested antigen.

Description

technical field [0001] The invention belongs to the field of food safety detection, and in particular relates to a detection method for harmful residues in food, in particular to a time-resolved fluorescent immunochromatographic test strip for quantitative detection of chloramphenicol and a preparation method and application thereof. Background technique [0002] Chloramphenicol is a broad-spectrum antibiotic, commonly used in the treatment of various infectious diseases in animals, and has a strong inhibitory effect on a variety of pathogenic bacteria. Chloramphenicol has serious side effects, which can inhibit the hematopoietic function of human bone marrow and cause human aplastic anemia and other diseases. Therefore, chloramphenicol residues in animal food pose a huge threat to human health. [0003] At present, the determination methods of chloramphenicol and its metabolites in food of animal origin are mainly physical and chemical detection methods. For example, chemic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/543G01N33/533G01N33/52
Inventor 洪霞杜霞秦悦
Owner NANJING YITE BIOTECH
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