32results about How to "Rapid detection of content" patented technology

The invention discloses an MOF (metal organic frameworks)-based magnetic nitrogen-doped carbon nano-tube material, a method for preparing the same and application of the MOF-based magnetic nitrogen-doped carbon nano-tube material. The MOF-based magnetic nitrogen-doped carbon nano-tube material can be used as an adsorption material. MOF are calcined at the high temperatures to obtain the MOF-basedmagnetic nitrogen-doped carbon nano-tube material. Carbon nano-tubes with large quantities of free N elements are generated on the surfaces of the MOF-based magnetic nitrogen-doped carbon nano-tube material while the appearance of the MOF is reserved. The MOF-based magnetic nitrogen-doped carbon nano-tube material, the method and the application have the advantages that the MOF are used as templates for the MOF-based magnetic nitrogen-doped carbon nano-tube material which is a magnetic carbon material prepared by the aid of the method, and are directly calcined at the high temperatures to obtain the magnetic carbon material; the carbon material is high in environmental stability as compared with MOF adsorption materials, and pi-pi conjugation can be formed by the carbon material and targetobjects by the aid of strong conjugation surfaces of the carbon nano-tubes; the MOF-based magnetic nitrogen-doped carbon nano-tube material is extremely high in magnetism as compared with commercialcarbon nano-tubes, and target object detection procedures can be simplified by the aid of MSPE (magnetic solid-phase extraction) methods; processes for preparing the MOF-based magnetic nitrogen-dopedcarbon nano-tube material are simple, and the MOF-based magnetic nitrogen-doped carbon nano-tube material which is a magnetic nitrogen-doped carbon nano-material is high in magnetism and super-high inenvironmental stability, has large specific surface areas, can be specifically bound with the target objects and has an excellent application prospect in the field of detection on the content of microcystin in lake water and the like.

The invention discloses a method for measuring the content of solanesol in tobaccos by using a UPLC (Ultra Performance Liquid Chromatography). The method comprises the following steps of: grinding the tobaccos into powder and weighing 50 mg of the tobacco powder; putting the weighed tobacco powder into a centrifuge tube with the capacity of 50 mL and adding 25 mL of a methanol solution; placing the centrifuge tube into an ultrasonic vibrator to be ultrasonically extracted for 10 min; taking 2 mL of an extracting solution and filtering the extracting solution by a filter membrane with the size of 0.22 micron to obtain clean liquid; and utilizing the ultra performance liquid chromatography to analyze to detect the content of the solanesol in the tobaccos. The method disclosed by the invention has the advantages of simple extraction method, convenience for detection operation, high flexibility and good repeatability, thereby rapidly and accurately detecting the content of the solanesol in tobacco samples to be detected.

The invention discloses a quick detector for quality and safety of tea. The detector is provided with a machine shell of which the upper surface is provided with a color comparison cuvette cover. Thedetector is characterized in that: a transmission type color comparison cuvette and a reflection type color comparison cuvette are arranged side by side under the color comparison cuvette cover of themachine shell; the transmission type color comparison cuvette is a rectangular tank body, one side surface of the tank body is provided with a light source / monochromator, and the other opposite sidesurface is provided with a photoelectric detector; and the reflection type color comparison cuvette is a cuboid of which the inside is provided with three T-shaped channel holes, channel openings of two side channel holes are provided with a light source / monochromator respectively, the lower part channel opening of the middle channel hole is provided with a photoelectric detector, and the lower part channel opening of the middle channel hole is provided with a chromatographic plate. The detector can be used for quickly and quantitatively detecting the contents of heavy metal and pesticide in tea in field and quickly detect the quality safety of tea in field.

The invention discloses a monoclonal antibody of glycinin and the application thereof. Monoclonal antibody of glycinin provided by the invention is made of anti-glycinin monoclonal antibody cell line 4B2 with preserving number being CGMCC No.2791. When being used for detecting the glycinin, the glycerin monoclonal antibody of the invention has the advantages that the defects, such as no reference conducted between different laboratories, low sensitivity and accuracy, and the repeated preparation for polyvalent antibody in an immune manner, existing in the traditional methods, such as SDS-PAGE and polyvalent antibody indirect ELISA; the detection accuracy is close to that obtained by adopting reversed-phase hplc method (RP-HPLC); the cost is very low; the requirement for detecting the content of glycinin in soybeans, bean pulp, and bean products in a high throughput manner by the feed industry, the food industry, and relevant enterprises; and the broad application prospect of quantitatively detecting antigenic substances of bean products and evaluating the hypersensitivity-inducing ability is possessed.

The invention relates to a method for detecting PAEs with molecular beacon fluorescence quantitative PCR. The method comprises the following steps that: cytosol which is extracted from carp liver and contains a receptor is combined with PAEs of different concentration to form compounds; the compounds are combined with bound DNA synthesized by specific primers to form ligand-receptor-DNA compounds; the ligand-receptor-DNA compounds are digested by exonuclease III and S1 nuclease; the products of the enzyme digestion are used as templates for molecular beacon fluorescence quantitative PCR amplification, and a regression equation between concentration of added PAEs and copy numbers of an initial template is obtained; according to the regression equation and a quantitative DNA standard curve,the relationship between the concentration of PAEs and Ct values is obtained through linear regression analysis, thereby enabling detection of PAEs of different concentration to be realized. The method provided in the invention has the characteristics of high sensitivity, low detection limit and strong specificity, is applicable to rapid detection of the content of PAEs in mass samples, and has agood application prospect.

The invention relates to a preparation method and application of a test strip for fast time-resolved fluorescent immunochromatography quantitative detection of amantadine. The test strip comprises three parts such as a Fusion 5 membrane, a cellulose nitrate membrane and water absorbent paper. According to the principle of the competition law, a time-resolved fluorescent microsphere is taken as an immune marker for accurate and fast quantitative detection of an antigen to be detected.

The invention discloses a method for detecting heavy metal in plants. The method comprises the following steps of (1) placing 2g of crashed and air-dried plant samples into a conical flask; adding water to moist; adding 25ml of nitric acid and 10mL of perchloric acid; standing for one day, and then heating until the plant samples generate white smoke; adding 2mL of hydrochloric acid after the plant samples are cooled; adding water until the constant volume is 50mL; and centrifuging and taking supernate; (2) placing the supernate into a separating funnel with a volume of 150ml; adding 2.0mL of an ascorbic acid resolution and 2.5mL of a potassium iodide solution; uniformly shaking and then accurately adding 5.00mL of methyl isobuthyl ketone; shaking for 1-2 minutes and standing for layering; and taking an organic phase for future measurement; (3) preparing a heavy metal standard curve; and (4) detecting by using a computer, and calculating a content of the heavy metal according to a standard curve. The method provided by the invention has the advantage of rapidly and accurately detecting the content of the heavy metal in the plants.

The invention belongs to the technical field of nano material preparation, and particularly relates to a preparation method and applications of MOF-based hydroxylated magnetic nitrogen-doped carbon nanotubes. The preparation method comprises the following steps: directly calcining MOF in a tubular furnace, and carrying out hydroxylation modification on the material by using a chemical means to prepare the hydroxylated magnetic nitrogen-doped carbon nanotube material. According to the invention, the preparation process is simple; the water dispersibility of the obtained hydroxylated magnetic nitrogen-doped carbon nanomaterial is improved on the basis that the structure stability, the high magnetism and the large specific surface area are still kept under the existing strong acid and alkaliconditions; and a large number of uncoordinated hydroxyl groups enable the material to have good selectivity on a carboxyl-containing target object, and when the material is used for monitoring the content change of auxin in tea leaves under the stress of heavy metals in soil, analysis and understanding of the growth mechanism of plants under the stress of heavy metals in soil are facilitated.

The invention discloses a method for rapidly detecting the content of B vitamins in pig intestinal contents / excrement. The method comprises the following steps: weighing cecum contents / excrement of pigs as a sample, adding a methanol / acetonitrile / water solution, and performing homogeneous extraction to obtain a sample solution; and performing UPLC-MS / MS-MRM detection analysis on the sample solution to finally obtain the content of eight B vitamins in the sample, wherein the eight B vitamins are vitamin B1, vitamin B2, nicotinic acid, nicotinamide, pyridoxal, pyridoxine, vitamin B9 and vitaminB12. According to the method, an MRM technology is utilized, an ultra-high performance liquid chromatography system and a mass spectrometer are combined, and the content of a plurality of B vitamins in the samples such as the pig intestinal contents and the excrement can be rapidly detected based on targeted metabonomics of an MRM method.

The invention relates to a preparation method and application of a quick test strip for testing chloramphenicol by adopting time-resolved fluorescence lateral flow immunoassay quantitative testing. The test strip is composed of a Fusion5 membrane, a nitrocellulose membrane and water absorption paper, according to the competition law principle, time-resolved fluorescence microspheres are taken as an immune marker, and accurate and quick quantitative testing can be conducted on a to-be-tested antigen.

The invention discloses a kit as well as a preparation method and application thereof, and relates to the technical field of medical diagnosis, the kit is used for detecting the content of a tissue plasminogen activator-plasminogen activator inhibitors 1 compound, the kit comprises a first working solution, a second working solution and a third working solution, the first working solution comprises a biotinylation coated antibody and a first buffer solution, and the second working solution comprises a second buffer solution and a third buffer solution; the biotinylated coated antibody is obtained by coupling biotin and a coated antibody; the second working solution comprises superparamagnetic particles coated with streptavidin and a second buffer solution; the third working solution comprises a labeled antibody labeled with an enzyme and a third buffer solution, and the labeled antibody labeled with the enzyme is obtained by coupling the enzyme activated by carbodiimide and N-hydroxysuccinimide with the labeled antibody; a luminescent substrate; and a cleaning solution. When the kit provided by the invention is used for detecting the content of the plasminogen activator-plasminogen activator inhibitor 1 compound in the tissue, the accuracy is high, and the stability is good.

The invention discloses a test strip for detecting lactic acid and a preparation method thereof. The test strip comprises a lactic acid reaction system, a first fixing layer formed on the lactic acidreaction system and a hydrophilic film covering the first fixing layer, wherein the lactic acid reaction system comprises a diffusion film formed between the U-shaped glue, a blood filtering film arranged below the diffusion film, a reaction film arranged below the blood filtering film, a second fixing layer which is arranged below the first fixing layer and used for fixing the blood filtering film and the reaction film, a bottom plate arranged below the second fixing layer, and a detection hole which is formed in the bottom plate and located below the reaction film. The test strip prepared bythe preparation method is matched with a dry biochemical analyzer to be used so that the content of lactic acid in body fluid in a human body or an animal body can be rapidly and quantitatively detected, and the detected result is well matched with the hospital inspection result.

The invention belongs to the technical field of a solid phase extraction adsorbent, and particularly relates to preparation and application of a magnetic metal organic framework nanosheet material. The preparation method comprises the following steps that 1, nanometer magnetic beads are dispersed in a mixed solution of polyvinylpyrrolidone and trichloromethane; mechanical stirring is performed for24h; the nanometer magnetic beads are collected and are cleaned by trichloromethane and methanol; modified nanometer magnetic beads are obtained and are then dispersed in the methanol for later use;2, the modified nanometer magnetic beads synthesized in the first step are dispersed in the methanol mixed solution containing copper nitrate and cobalt nitrate; after uniform dispersion, a methanol solution containing 2-methylimidazole is added under the ultrasonic concussion condition; ultrasonic concussion reaction is performed for 1h; then, the methanol is used for cleaning; the magnetic metalorganic framework nanosheet material is obtained and is dispersed in methanol for later use. The nanosheet material has more open surfaces and rich metal sites; fast separation can be realized afterthe adsorption.

The invention relates to a device for quickly detecting the content of nicotine in tobacco and a use method for the device. The device for quickly detecting the content of the nicotine in the tobaccocomprises a closed collecting tank for holding quantitative water, as well as a first air inlet and a second air inlet which are formed in the closed collecting tank, wherein an inner tube is insertedinto the first air inlet; a cigarette is inserted into the outer end of the inner tube; the second air inlet is connected with one end of an adjusting air pump. The device disclosed by the inventionhas the characteristics of simple operation, quick treatment, low cost and the like, and can be used for quickly detecting the content of the nicotine in the tobacco.

The invention relates to quantum-dot-based paper chips and a preparation method thereof, as well as application of the quantum-dot-based paper chips in glucose detection. The preparation method of thequantum-dot-based paper chips comprises the following steps: S1, drying porcine blood, and grinding the dried porcine blood into porcine blood powder; S2, dissolving the porcine blood, carrying out hydrothermal reaction at 150-200 DEG C for 10-18 hours, carrying out centrifuging so as to obtain liquid supernatant, filtering the liquid supernatant, and carrying out diluting so as to obtain a carbon quantum dot solution for standby application; S3, partially coating paper chips with hydrophobic matrices so as to form hydrophobic areas, and leaving the uncoated parts as hydrophilic areas; and S4, adding substrates of horseradish peroxidase and the carbon quantum dot solution into the hydrophobic areas, carrying out drying, adding a glucose oxidase solution, carrying out drying, and adding the substrates of horseradish peroxidase and the carbon quantum dot solution again so as to obtain the quantum-dot-based paper chips. The quantum-dot-based paper chips provided by the invention have activity of horseradish-like peroxidase, so that, the quantum-dot-based paper chips can be used for quickly detecting content of glucose; moreover, the detection results are more reliable and accurate, low in cost, high in application values and good in application prospects.

The invention relates to a new method for high-throughput detection of isoamyl alcohol produced by microorganisms based on a double-enzyme coupling reaction, wherein the isoamyl alcohol oxidase is expressed by recombinant Pichia pastoris. It specifically relates to the coupling of isoamyl alcohol oxidase and peroxidase to react with isoamyl alcohol in a corresponding buffer solution to produce quinone imine, a color-developing substance. The double-enzyme coupling reaction was carried out in a 96-well plate, and the absorbance at 500 nm was read with a microplate reader. The absorbance value at 500nm of the isoamyl alcohol and double-enzyme coupling reaction product is linearly related to the concentration of isoamyl alcohol, and the concentration can be calculated according to the standard curve, and high-throughput detection can be performed using a microplate reader and a 96 microwell plate.

The invention relates to a novel high-throughput detection method for microbial production for dibasic acid based on a coupled enzymatic reaction, and in particular to a method for generating a fluorescent substrate Resorufin by virtue of coupling between dibasic acid oxidase and peroxidase, and reaction with dibasic acid in corresponding buffering solution. According to the method, the coupled enzymatic reaction is performed in a 96-mesh black microporous plate, and a fluorescent value at a place of Ex / Em 490 / 585 (nm) is read by an enzymatic analyzer; and the fluorescent value of the product generated by the coupled reaction between the dibasic acid and the both enzymes at the place of Ex / Em 490 / 585 (nm) forms a linear relationship with the concentration of the product, the concentration can be calculated according to a standard curve, and high-throughput detection is performed by the enzymatic analyzer and the 96-mesh black microporous plate.

The invention relates to a method for preparing a cyproheptadine hydrochloride rapid time-resolved fluorescence lateral flow immunoassay quantitative detection test strip and application thereof. Thetest strip consists of a Fusion 5 membrane, a nitrocellulose membrane and absorbent paper, and time-resolved fluorescence microspheres are utilized as an immune marker through the principle of a competition method to carry out accurate and rapid quantitative detection on antigens to be detected.

The present invention relates to the technical field of plant effective component analysis, more particularly to a method for detecting tannin in mulberry leaf. The method specifically comprises: (1) taking a tannin standard substance, dissolving with a dilution liquid to prepare a tannin standard solution with a known concentration, carrying out injection analysis according to the tannin standard solution, and performing linear regression by corresponding the peak area to the tannin concentration to obtain a standard curve; and (2) taking a mulberry leaf sample, dissolving with a dilution liquid, filtering to obtain a mulberry leaf extract, carrying out injection analysis to obtain the peak area of the mulberry leaf extract; and obtaining the tannin content in the mulberry leaf sample according to the standard curve obtained in the step (1). According to the present invention, the method has advantages of simple operation and high precision, and can rapidly detect the tannin content in the mulberry leaf sample.

The invention discloses a cyclosporine conjugate and a preparation method and application thereof. The cyclosporine conjugate is as shown in general formula (I), wherein n is the molecular number of cyclosporine combined with one bovine serum albumin molecule and is an integer ranging from 1 to 20, BSA is bovine serum albumin, and the molecular weight range is 6.6-6.9KDa. The theophylline conjugate is formed by coupling cyclosporine hapten with carrier substance bovine serum albumin or ovalbumin generating immunogenicity. The preparation method includes: connecting and combining cyclosporine with the carrier substance generating immunogenicity to form the cyclosporine conjugate capable of inducing animal systems to generate antibodies. The cyclosporine conjugate immunizes New Zealand whiterabbits to prepare antiserum with the titer reaching 50,000, and the lowest detection limit of the antiserum is 20ppb. The cyclosporine conjugate is simple in method, fast, accurate and high in specificity and lays a foundation for the enzyme linked immunosorbent assay kit of cyclosporine.

The invention belongs to the technical field of food analysis, and discloses a method for rapidly detecting resveratrol in food. Firstly, carbon quantum dots are synthesized from biomass raw materialsthrough a hydrothermal method, and the carbon quantum dots and resveratrol are used for rapidly identifying and analyzing resveratrol in food by utilizing specific response between the obtained carbon quantum dots and resveratrol. The detection material involved in the method is safe and simple in synthesis, low in cost, free of complex pretreatment and extraction steps and rapid and accurate indetection, especially, rapid determination of resveratrol in a hydrophobic food matrix can be realized, and a brand-new thought for rapid and efficient detection of resveratrol in food can be provided.

The invention relates to an HMGB1 (High Mobility Group Box 1) detection kit and preparation method thereof. The HMGB1 detection kit comprises Reagent 1 and Reagent 2, wherein the Reagent 1 comprises HMGB1 antibody-labeled latex microspheres, a suspending agent, bovine serum albumin and a first buffer solution; the Reagent 2 comprises sodium chloride, a dispersing agent, a surfactant and a second buffer solution. According to the HMGB1 detection kit and preparation method thereof, the HMGB1 detection kit is a kit for detecting HMGB1 based on latex enhanced immunoturbidimetry; antigen and antibody reactions are carried out in a homogeneous reaction system when the HMGB1 detection kit is used; the absorbance value of a reaction solution is directly determined to reflect the concentration of ato-be-tested antigen after antigens bound to antibodies; and the HMGB1 detection kit has fast detection speed, low cost, time and labor saving, good stability and repeatability, and can truly reflectthe content of to-be-tested substances.

The invention relates to a novel method of coupled enzymatic reaction-based high-flux detection of ethanol and C3-C6 n-alkanol production capacity of microbes. The novel method is characterized in that alcohol oxidase and catalase are coupled and the double-enzyme conjugate and ethanol or C3-C6 n-alkanol undergo a reaction in a corresponding buffer solution to produce red quinone imide; the double-enzyme coupling reaction is carried out in a 96-hole enzyme label plate and a light absorption value at the wavelength of 500nm is read by a microporous read device; a light absorption value at the wavelength of 500nm and a concentration of the reaction product of ethanol, C3-C6 n-alkanol and the double-enzyme conjugate are linearly dependent; according to a standard curve, concentration calculation is realized; and through the microporous read device and the 96-hole enzyme label plate, high-flux detection is realized.

The invention relates to a novel method for producing isoamyl alcohol based on a high flux detection microbe of a double-enzyme coupling reaction. Isoamyl alcohol oxidase is obtained through expression of recombinant pichia pastoris. Specifically speaking, isoamyl alcohol oxidase is coupled with peroxidase, reactions with isoamyl alcohol are carried out in corresponding buffer solutions, and the chromogenic substance quinone-imine is produced; the double-enzyme coupling reaction is carried out in a 96 microwell plate, and a microplate reader is employed to read the light absorption value at 500 nm; since a linear relation is formed between the light absorption value of the resultant of the coupling reaction of isoamyl alcohol with the double enzymes at 500 nm and the concentration of isoamyl alcohol, the concentration can be calculated according to a standard curve, and the microplate reader and the 96 microwell plate are used for high flux detection.

The invention relates to a preparation method and an application of an SEI monoclonal antibody. The method comprises steps as follows: recombinant SEI immunizes BALB / c mice, splenocytes of the mice are directly fused with myeloma cells, an advantageous hybridoma cell strain producing the SEI monoclonal antibody is screened with the indirect ELISA (enzyme linked immunosorbent assay), and the SEI monoclonal antibody is obtained. The SEI monoclonal antibody prepared with the method is high in titer, stable in property and high in specificity, can be applied to reagent strips or kits, can quickly detect the content of SEI in samples, can be used for establishing an immunological detection method for the SEI and has bright application prospect.

The invention relates to a preparation method and application of a quick time-resolution fluorescence immunochromatography quantitative detection test strip for progesterone. The test strip is composed of a Fushion5 membrane, a nitrocellulose membrane and water-absorbing paper. Through a competition law principle, a time-resolution fluorescence microsphere is used as an immune marker, and a to-be-tested antigen is accurately, quickly and quantitatively detected.

The invention discloses a detection method and a detection reagent of fluoroquinolone antibiotics. The method comprises the steps of (1) feeding divalent heavy metal ion solution into a sample to be detected, and carrying out reaction to obtain antibiotic-heavy metal complex solution; (2) selecting a glassy carbon substrate electrode as a working electrode, testing the working electrode within the electric potential range of -0.9V to 0.6V by a voltammetry to obtain a current response curve, and determining oxidation peak response value or reduction peak response value; and (3) calibrating the concentration of the fluoroquinolone antibiotics in the sample to be detected according to the oxidation peak response value or reduction peak response value obtained in the step (2) as well as the measured standard curve. The detection reagent is buffer solution containing divalent heavy metal ions, and has the pH value within the range of 3.6-5.6. The method provided by the invention can be used for detecting the trace fluoroquinolone antibiotics in the sample, and is accurate in detection, rapid in speed and high in sensitivity.