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Quantum-dot-based paper chips and preparation method thereof, and application of quantum-dot-based paper chips in glucose detection

A paper chip and quantum dot technology, applied in the detection of glucose, based on the quantum dot paper chip and its preparation field, can solve the problems of high cost, blood sugar pollution, etc., achieve low cost, reliable and accurate detection results, and good application value and foreground effects

Active Publication Date: 2019-07-23
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the defects and deficiencies of pollution and high cost in blood glucose detection in the prior art, and provide a preparation method based on quantum dot paper chips

Method used

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  • Quantum-dot-based paper chips and preparation method thereof, and application of quantum-dot-based paper chips in glucose detection
  • Quantum-dot-based paper chips and preparation method thereof, and application of quantum-dot-based paper chips in glucose detection
  • Quantum-dot-based paper chips and preparation method thereof, and application of quantum-dot-based paper chips in glucose detection

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Embodiment 1

[0038] The invention provides a paper chip based on quantum dots, the preparation method of which is as follows.

[0039] (1) Preparation of horseradish peroxidase-like carbon quantum dots

[0040] 1. Preparation of pig blood powder: buy pig blood from the market, put it on filter paper to absorb the liquid on the surface, then put it in an oven to dry, put it in a mortar and grind it to get powder after drying, and weigh it.

[0041] 2. Preparation of carbon quantum dots: Weigh 1g of pig blood powder, add 100mL of deionized water, blow and suck repeatedly to make the solution uniform, transfer to a polytetrafluoroethylene-lined reactor, react at 180°C for 12 hours, take out the reaction after cooling Centrifuge the reaction solution at 8,000 rpm at room temperature for 10 minutes to remove the precipitate, take the supernatant, filter the supernatant with a 0.45 μm filter membrane, and store the solution in a refrigerator at 4°C.

[0042] Such as figure 1 , is the ultraviol...

Embodiment 2

[0053] This embodiment provides a paper chip based on quantum dots, the preparation method of which is as follows.

[0054] (1) Preparation of horseradish peroxidase-like carbon quantum dots

[0055] 1. Preparation of pig blood powder: buy pig blood from the market, put it on filter paper to absorb the liquid on the surface, then put it in an oven to dry, put it in a mortar and grind it to get powder after drying, and weigh it.

[0056] 2. Preparation of carbon quantum dots: Weigh 1g of pig blood powder, add 100mL of deionized water, blow and suck repeatedly to make the solution uniform, transfer to a polytetrafluoroethylene-lined reactor, react at 150°C for 20 hours, take out the reaction after cooling Centrifuge the reaction solution at 8,000 rpm at room temperature for 10 minutes to remove the precipitate, take the supernatant, filter the supernatant with a 0.45 μm filter membrane, and store the solution in a refrigerator at 4°C.

[0057] (2) The production of the paper ch...

Embodiment 3

[0059] This embodiment provides a paper chip based on quantum dots, the preparation method of which is as follows.

[0060] (1) Preparation of horseradish peroxidase-like carbon quantum dots

[0061] 1. Preparation of pig blood powder: buy pig blood from the market, put it on filter paper to absorb the liquid on the surface, then put it in an oven to dry, put it in a mortar and grind it to get powder after drying, and weigh it.

[0062] 2. Preparation of carbon quantum dots: Weigh 1g of pig blood powder, add 100mL of deionized water, blow repeatedly to make the solution uniform, transfer to a polytetrafluoroethylene-lined reactor, react at 200°C for 10 hours, take out the reaction after cooling Centrifuge the reaction solution at 8,000 rpm at room temperature for 10 minutes to remove the precipitate, take the supernatant, filter the supernatant with a 0.45 μm filter membrane, and store the solution in a refrigerator at 4°C.

[0063] (2) The production of the paper chip is con...

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Abstract

The invention relates to quantum-dot-based paper chips and a preparation method thereof, as well as application of the quantum-dot-based paper chips in glucose detection. The preparation method of thequantum-dot-based paper chips comprises the following steps: S1, drying porcine blood, and grinding the dried porcine blood into porcine blood powder; S2, dissolving the porcine blood, carrying out hydrothermal reaction at 150-200 DEG C for 10-18 hours, carrying out centrifuging so as to obtain liquid supernatant, filtering the liquid supernatant, and carrying out diluting so as to obtain a carbon quantum dot solution for standby application; S3, partially coating paper chips with hydrophobic matrices so as to form hydrophobic areas, and leaving the uncoated parts as hydrophilic areas; and S4, adding substrates of horseradish peroxidase and the carbon quantum dot solution into the hydrophobic areas, carrying out drying, adding a glucose oxidase solution, carrying out drying, and adding the substrates of horseradish peroxidase and the carbon quantum dot solution again so as to obtain the quantum-dot-based paper chips. The quantum-dot-based paper chips provided by the invention have activity of horseradish-like peroxidase, so that, the quantum-dot-based paper chips can be used for quickly detecting content of glucose; moreover, the detection results are more reliable and accurate, low in cost, high in application values and good in application prospects.

Description

technical field [0001] The invention belongs to the technical field of medical chemical detection, and in particular relates to a paper chip based on quantum dots, a preparation method thereof and an application in detecting glucose. Background technique [0002] The main external manifestation of diabetes is abnormally high blood sugar levels, but in addition, it has negative effects on the cardiovascular system, nervous system, and major organs such as the heart, liver, and kidneys to varying degrees. A series of complications not only bring a heavy economic burden to patients and society, but also an important factor causing death. According to the survey organized by the Chinese Diabetes Association, the incidence of diabetes in my country is as high as 9.7%, and diabetes is a group of metabolic diseases characterized by hyperglycemia. Blood sugar is the only standard for detecting diabetes, and the daily detection of blood sugar has important guiding significance for d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/54C12Q1/26
CPCC12Q1/54C12Q1/26
Inventor 何绮怡赵肃清杨慧怡沈定陈莹珊方岩雄
Owner GUANGDONG UNIV OF TECH
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