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Cyproheptadine hydrochloride rapid time-resolved fluorescence lateral flow immunoassay quantitative detection test strip

A technology of time-resolved fluorescence and cyproheptadine hydrochloride, which is applied to the analysis of materials, measuring devices, instruments, etc., can solve the problems of low sensitivity, detection influence, and large batch-to-batch difference, and achieve high sensitivity

Inactive Publication Date: 2019-05-28
DANYANG YITAI BIOTECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The rapid detection products developed based on colloidal gold labeling technology have problems such as qualitative or semi-quantitative, and large batch-to-batch differences; although the batch-to-batch difference of colored latex microspheres has improved, the sensitivity is still low and can only be qualitative or semi-quantitative ; The sensitivity of immunochromatography based on fluorescein labeling technology has been greatly improved, and quantitative detection can also be performed, but because the sample contains a high fluorescent background signal and the stock shift is small, it will have a greater impact on the detection

Method used

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  • Cyproheptadine hydrochloride rapid time-resolved fluorescence lateral flow immunoassay quantitative detection test strip
  • Cyproheptadine hydrochloride rapid time-resolved fluorescence lateral flow immunoassay quantitative detection test strip
  • Cyproheptadine hydrochloride rapid time-resolved fluorescence lateral flow immunoassay quantitative detection test strip

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Experimental program
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Embodiment Construction

[0034] 1. Preparation of Components of Test Strips

[0035] 1.1 Preparation of quality control microspheres Preparation of quality control microspheres coated with biotinylated γ-globulin (BGG)

[0036] ⑴ Preparation of biotinylated BGG

[0037] with 0.1 M NaCNBH 3 BGG (purchased from Pel-Freez Biological) was prepared into a 10 mg / mL solution, and Biotin-X-X-NHS (N-hydroxysuccinimide modified biotin, manufacturer: SIGMA, Product number: B3295) solution to 16.2 mg / mL, add Biotin-X-X-NHS solution to BGG solution according to the amount of 1 mg BBG protein and 5.4 μl Biotin-X-X-NHS solution, mix well and place overnight at 4°C. Dialysis was used to remove free unreacted biotin, and the dialysate was biotin-labeled protein dialysate buffer (0.1 M Tris, 0.3 M Nacl, 0.005 MEDTA-Na-2H 2 0, pH8.0). After dialysis, the protein concentration was determined by BCA method.

[0038] ⑵ Use the above-mentioned labeled protein to coat the aldehyde-modified fluorescent microspheres

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Abstract

The invention relates to a method for preparing a cyproheptadine hydrochloride rapid time-resolved fluorescence lateral flow immunoassay quantitative detection test strip and application thereof. Thetest strip consists of a Fusion 5 membrane, a nitrocellulose membrane and absorbent paper, and time-resolved fluorescence microspheres are utilized as an immune marker through the principle of a competition method to carry out accurate and rapid quantitative detection on antigens to be detected.

Description

technical field [0001] The invention belongs to the field of food safety detection, and in particular relates to a detection method for harmful residues in food, in particular to a time-resolved fluorescent immunochromatographic test strip for quantitative detection of cyproheptadine hydrochloride, a preparation method and application thereof. Background technique [0002] Cyproheptadine hydrochloride (Cyproheptadine hydrochloride, molecular formula: C 21 h 21 N·HCl) is an antihistamine with anti-5-HT, blocking H1 receptor and anticholinergic effects, mainly used in the treatment of urticaria, eczema, contact dermatitis, skin itching, allergic rhinitis, etc. Its common side effects are mild to moderate drowsiness, dizziness, fatigue, dry mouth and so on. Consumption of animal-derived products containing clonidine hydrochloride and cyproheptadine hydrochloride residues is harmful to human health. The Ministry of Agriculture issued Announcement No. 1519 on December 27, 2010...

Claims

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Application Information

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IPC IPC(8): G01N33/533G01N33/558
Inventor 杜霞洪霞立雯馨
Owner DANYANG YITAI BIOTECH DEV
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