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Detection method and detection reagent of fluoroquinolone antibiotics

A fluoroquinolone and detection method technology, applied in the field of antibiotic analysis and detection, can solve the problems of low sensitivity, complicated electrode preparation process, etc., and achieve the effects of high sensitivity, simple preparation process, and simple and fast test method

Inactive Publication Date: 2014-10-29
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the above defects or improvement needs of the prior art, the present invention provides a detection method and detection reagent for fluoroquinolone antibiotics, the purpose of which is to simply and quickly detect low-concentration fluoroquinolones in solution by electrochemical methods , so as to solve the technical problems of time-consuming, complicated electrode preparation process or low sensitivity in the existing detection methods

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  • Detection method and detection reagent of fluoroquinolone antibiotics
  • Detection method and detection reagent of fluoroquinolone antibiotics
  • Detection method and detection reagent of fluoroquinolone antibiotics

Examples

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Embodiment 1

[0039] A detection reagent for fluoroquinolone antibiotics containing divalent heavy metal ions 10 -2 The acetic acid buffer solution of mol / L; Its pH value is 3.6; Described divalent heavy metal ion is copper ion.

[0040] With described fluoroquinolone antibiotic detection reagent, detect ofloxacin, the steps are as follows:

[0041] (1) Add the fluoroquinolone antibiotic detection reagent to the sample to be tested, mix evenly, react for 10 minutes, adjust the pH value to 3.6, and obtain the antibiotic-heavy metal complex solution, wherein the concentration of the divalent heavy metal ion is 10 -4 mol / L. The sample to be tested is a chicken feed sample obtained by the standard recovery method, wherein the concentration of the standard ofloxacin added is 5 × 10 -6 mol / L.

[0042] (2) Select the glassy carbon electrode as the working electrode, the calomel electrode as the reference electrode, and the platinum electrode as the auxiliary electrode, and use the electrochemic...

Embodiment 2

[0050] A detection reagent for fluoroquinolone antibiotics containing divalent heavy metal ions 10 -2 mol / L acetate buffer solution; its pH value is 3.6; the divalent heavy metal ion is cadmium ion.

[0051] With described fluoroquinolone antibiotic detection reagent, detect ciprofloxacin, the steps are as follows:

[0052] (1) Add the fluoroquinolone antibiotic detection reagent to the sample to be tested, mix evenly, react for 3 minutes, adjust the pH value to 3.6, and obtain the antibiotic-heavy metal complex solution, wherein the concentration of the divalent heavy metal ion is 10 -4 mol / L. The sample to be tested is ciprofloxacin hydrochloride tablet, the specification is 250mg / tablet.

[0053] (2) Select the glassy carbon electrode modified by nanomaterials as the working electrode, the calomel electrode as the reference electrode, and the platinum electrode as the auxiliary electrode, and use linear sweep voltammetry to perform step (1) in the potential range of -0.9V...

Embodiment 3

[0062] A detection reagent for fluoroquinolone antibiotics containing 5×10 divalent heavy metal ions -3 mol / L acetate buffer solution; its pH value is 5.6; the divalent heavy metal ion is cadmium ion.

[0063] With described fluoroquinolone antibiotic detection reagent, detect ciprofloxacin, the steps are as follows:

[0064] (1) Add the fluoroquinolone antibiotic detection reagent to the sample to be tested, mix evenly, react for 5 minutes, adjust the pH value to 5.6, and obtain the antibiotic-heavy metal complex solution, wherein the concentration of the divalent heavy metal ion is 5 × 10 -5 mol / L. The sample to be tested is ciprofloxacin hydrochloride tablet, the specification is 250mg / tablet.

[0065] (2) Select the glassy carbon electrode as the working electrode, the calomel electrode as the reference electrode, and the platinum electrode as the auxiliary electrode, and use linear sweep voltammetry to test the antibiotics obtained in step (1) in the potential range of ...

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Abstract

The invention discloses a detection method and a detection reagent of fluoroquinolone antibiotics. The method comprises the steps of (1) feeding divalent heavy metal ion solution into a sample to be detected, and carrying out reaction to obtain antibiotic-heavy metal complex solution; (2) selecting a glassy carbon substrate electrode as a working electrode, testing the working electrode within the electric potential range of -0.9V to 0.6V by a voltammetry to obtain a current response curve, and determining oxidation peak response value or reduction peak response value; and (3) calibrating the concentration of the fluoroquinolone antibiotics in the sample to be detected according to the oxidation peak response value or reduction peak response value obtained in the step (2) as well as the measured standard curve. The detection reagent is buffer solution containing divalent heavy metal ions, and has the pH value within the range of 3.6-5.6. The method provided by the invention can be used for detecting the trace fluoroquinolone antibiotics in the sample, and is accurate in detection, rapid in speed and high in sensitivity.

Description

technical field [0001] The invention belongs to the field of antibiotic analysis and detection, and more specifically relates to a detection method for fluoroquinolone antibiotics. Background technique [0002] In recent years, antibiotic residues have become an environmental issue of public concern. Taking fluoroquinolone antibiotics as an example, due to incomplete absorption by the body, the drug content discharged into water and soil media in situ or metabolized form accounts for about 80% of the total usage. These undecomposed antibiotics enter the environment through wastewater or livestock manure, which will cause water and soil pollution, and eventually cause harm to humans. [0003] Antibiotics, especially broad-spectrum fluoroquinolone antibiotics such as ofloxacin and ciprofloxacin, have good bactericidal effects, so they have been widely used in animal husbandry. However, their residues in animal husbandry products and ecological environment problems are of grea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48
Inventor 张敬东单君吕松松
Owner HUAZHONG UNIV OF SCI & TECH
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