The invention relates to a constant temperature reaction-based method for detecting microRNA in a solution to be detected. The method comprises that an electrode is immersed in a first DNA fragment molecular solution, undergoes a reaction at a temperature of 37 DEG C for 6h, then is immersed in a mercapto hexanol solution, undergoes a reaction for 1h, then is immersed in a silver nanoparticle solution, undergoes a reaction for 1h, then is immersed in a second DNA fragment molecular solution and undergoes a reaction for 0.5h, a microRNA standard liquid is prepared, the electrode is immersed in the microRNA standard liquid, a third DNA fragment molecule, a Klenow fragment and dNTPs are added into the microRNA standard liquid, the electrode undergoes a reaction in the mixed liquid at a temperature of 37 DEG C for 1h, then is immersed in a Nt. BbvCI solution and undergoes a reaction for 1h, wherein in the detection, a linear scanning voltammetry method is used, a standard curve graph is drawn, an electrical signal of the liquid to be detected is acquired by the above processes, and the electrical signal and the standard curve graph are compared so that a microRNA concentration of the liquid to be detected is obtained. The constant temperature reaction-based method has the advantages of simple processes, high sensitivity, good specificity, low background signal and wide application range.