Electrochemical Detection Method and Application of Reduced Graphene Oxide Catalyzed Silver Deposition

A graphene catalyzed silver and detection method technology, which is applied in the direction of material electrochemical variables, measuring devices, scientific instruments, etc., can solve the problems of cumbersome operation, and achieve the effects of safe detection process, wide application, and easy miniaturization

Active Publication Date: 2017-03-15
GUANGXI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method can realize the detection of carcinoembryonic antigen at low concentration, but the operation is cumbersome, and the silver deposition needs to be mediated by metal nanoparticles

Method used

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  • Electrochemical Detection Method and Application of Reduced Graphene Oxide Catalyzed Silver Deposition
  • Electrochemical Detection Method and Application of Reduced Graphene Oxide Catalyzed Silver Deposition
  • Electrochemical Detection Method and Application of Reduced Graphene Oxide Catalyzed Silver Deposition

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Experimental program
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Effect test

Embodiment 1

[0049] Example 1. Quantitative detection of the specific activity of dam methylase based on the electrochemical detection method of reduced graphene oxide catalyzed silver deposition

[0050] figure 1It is a schematic diagram of the electrochemical detection method based on reduced graphene oxide catalyzed silver deposition of the present invention for the detection of methylase.

[0051] 1) Preparation of rGO

[0052] Weigh 10 mg of graphene oxide and disperse it in 10 mL of water, and intensify ultrasonication for 1 hour to obtain a uniformly dispersed graphene oxide solution. Add 10 ml of water, 1 mL of ammonia water and 30 μL of hydrazine hydrate to the solution, mix well, and react in a water bath at 60° C. for 4 hours. After the product was centrifuged and washed twice, it was redispersed in 10 mL of water to obtain an rGO solution (concentration: 1 mg / mL).

[0053] figure 2 Infrared spectra of graphene oxide and rGO.

[0054] 2) Preparation of working electrode

...

Embodiment 2

[0066] Establish standard curve according to the method in embodiment 1, difference is:

[0067] Immerse the working electrode treated in step 2) into 20 μL of dam methylase (standard specific activity is 1 U / mL), DpnI restriction endonuclease (50 U / mL), S-adenosylmethionine (320 μM) In Tris-EDTA buffer, react at 37°C for 2 hours to obtain the treated working electrode;

[0068] Perform detection according to the operations of B and C in step 3) in turn, and obtain a silver stripping current response value of 2.2 μA. According to the standard curve I(μA)=0.3883X+1.8528, the corresponding specific activity of dam methylase in the actual sample solution is 0.89U / mL.

[0069] The measured specific activity of dam methylase (0.89U / mL) was close to the standard specific activity of 1U / mL. This shows that the electrochemical detection method based on reduced graphene oxide catalyzed silver deposition provided by the present invention can accurately measure the specific activity of...

Embodiment 3

[0070] Embodiment 3, establish standard curve according to the method in embodiment 1, difference is:

[0071] Immerse the working electrode treated in step 2) into 20 μL containing dam methylase (standard specific activity is 0.5 U / mL), Dpn I restriction endonuclease (50 U / mL), S-adenosylmethionine (320 μM ) in Tris-EDTA buffer solution, reacted at 37°C for 2 hours to obtain the treated working electrode;

[0072] Perform detection according to the operations of B and C in step 3) in turn, and obtain a silver stripping current response value of 2.08 μA. According to the standard curve I(μA)=0.3883X+1.8528, the corresponding specific activity of dam methylase in the actual sample solution is 0.59U / mL.

[0073] The measured specific activity of dam methylase (0.59U / mL) was close to the standard specific activity of 0.5U / mL. This shows that the electrochemical detection method based on reduced graphene oxide catalyzed silver deposition provided by the present invention can acc...

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Abstract

The invention provides an electrochemical detection method for catalytic silver deposit of reducing type oxidized graphene, as well as applications thereof. The method comprises the following steps: modifying a DNA probe, which can be identified specifically by a to-be-detected object, on a gold electrode, to obtain a working electrode; reacting the working electrode, the to-be-detected object with known specific activity, and standard sample solution of substances capable of cutting the DNA probe, which can be identified specifically by a to-be-detected object, into oligonucleotides single chains, loading the reacted electrode with reducing type oxidized graphene, performing silver deposit reaction together with silver ion-containing solution, dissolving out silver monomer deposited on the working electrode, determining the dissolution volt-ampere current value by utilizing a linear sweep voltammetry, and drawing a standard cure; according to the standard cure, calculating to obtain the specific activity of the to-be-detected sample. The established electrochemical detection method has the advantages of being labeling-free, safe for detection process, simple to operate, easy to miniature, wide to apply and the like.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to an electrochemical detection method based on reduced graphene oxide catalyzed silver deposition and its application. Background technique [0002] Graphene is a two-dimensional carbon nanomaterial. Its unique electronic structure enables it to have high electron mobility, large specific surface area, and excellent mechanical, thermal and catalytic properties. It is widely used in small biological molecules, nucleic acids, enzymes, proteins, In the detection of target objects such as cells and metal ions. As a kind of graphene derivatives, reduced graphene oxide (rGO) is easier to combine with oligonucleotide single strands through π-π conjugation due to fewer oxygen-containing groups on its surface. Lin et al. (Biosensors and Bioelectronics 52 (2014) 153–158) reported a modified reduced graphene oxide on the surface of a glassy carbon electrode by electr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/48G01N27/327
Inventor 赵永祥苏婧卢小玲梁颖黄勇李桂银
Owner GUANGXI MEDICAL UNIVERSITY
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