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Time-resolution fluorescence immunochromatography quantitative detection test strip for detecting progesterone

A technology of time-resolved fluorescence and immunochromatography, which is applied in measuring devices, analytical materials, instruments, etc., can solve problems such as low sensitivity, detection influence, and large batch-to-batch differences, and achieve the effect of high sensitivity

Inactive Publication Date: 2019-05-28
ZHENJIANG YITE BIOTECH DEV CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The rapid detection products developed based on colloidal gold labeling technology have problems such as qualitative or semi-quantitative, and large batch-to-batch differences; although the batch-to-batch difference of colored latex microspheres has improved, the sensitivity is still low and can only be qualitative or semi-quantitative ; The sensitivity of immunochromatography based on fluorescein labeling technology has been greatly improved, and quantitative detection can also be performed, but because the sample contains a high fluorescent background signal and the stock shift is small, it will have a greater impact on the detection

Method used

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  • Time-resolution fluorescence immunochromatography quantitative detection test strip for detecting progesterone
  • Time-resolution fluorescence immunochromatography quantitative detection test strip for detecting progesterone
  • Time-resolution fluorescence immunochromatography quantitative detection test strip for detecting progesterone

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Experimental program
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Embodiment Construction

[0034] 1. Preparation of Components of Test Strips

[0035] 1.1 Preparation of quality control microspheres Preparation of quality control microspheres coated with biotinylated γ-globulin (BGG)

[0036] ⑴ Preparation of biotinylated BGG

[0037] with 0.1 M NaCNBH 3 BGG (purchased from Pel-Freez Biological) was prepared into a 10 mg / mL solution, and Biotin-X-X-NHS (N-hydroxysuccinimide modified biotin, manufacturer: SIGMA, Product number: B3295) solution to 16.2 mg / mL, add Biotin-X-X-NHS solution to BGG solution according to the amount of 1 mg BBG protein and 5.4 μl Biotin-X-X-NHS solution, mix well and place overnight at 4°C. Dialysis was used to remove free unreacted biotin, and the dialysate was biotin-labeled protein dialysate buffer (0.1 M Tris, 0.3 M Nacl, 0.005 MEDTA-Na-2H 2 0, pH8.0). After dialysis, the protein concentration was determined by BCA method.

[0038] ⑵ Use the above-mentioned labeled protein to coat the aldehyde-modified fluorescent microspheres

[0...

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Abstract

The invention relates to a preparation method and application of a quick time-resolution fluorescence immunochromatography quantitative detection test strip for progesterone. The test strip is composed of a Fushion5 membrane, a nitrocellulose membrane and water-absorbing paper. Through a competition law principle, a time-resolution fluorescence microsphere is used as an immune marker, and a to-be-tested antigen is accurately, quickly and quantitatively detected.

Description

technical field [0001] The invention belongs to the field of food safety detection, and in particular relates to a detection method for harmful residues in food, in particular to a time-resolved fluorescent immunochromatographic test strip for quantitative detection of progesterone and a preparation method and application thereof. Background technique [0002] Progesterone, also known as progesterone, progesterone, progesterone, luteinizing hormone, progestin, progestin, or progesterone, is a hormone involved in the female menstrual cycle, pregnancy, and embryonic effects in humans and other animals. of steroids. Progesterone is a female hormone involved in the female menstrual cycle in humans and other animals, supporting pregnancy and embryo formation. Progesterone belongs to a class of hormones called progestins and is the most important progestin in humans. Hormone drugs can improve the feed conversion rate of animal food, and then achieve the purpose of weight gain, b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533G01N33/558G01N33/543G01N33/577
Inventor 洪霞杜霞张淑雅
Owner ZHENJIANG YITE BIOTECH DEV CO LTD
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