Method for rapidly measuring content of solanesol in tobaccos by using UPLC (Ultra Performance Liquid Chromatography)

A technique for the rapid determination of midsolanesol, applied in the field of liquid chromatography analysis, to achieve the effects of simple processing method, fast analysis speed and accurate quantification

Inactive Publication Date: 2012-06-27
GUIZHOU TOBACCO SCI INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no technical patent application for the detection method of solanesol content in tobacco leaves

Method used

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  • Method for rapidly measuring content of solanesol in tobaccos by using UPLC (Ultra Performance Liquid Chromatography)
  • Method for rapidly measuring content of solanesol in tobaccos by using UPLC (Ultra Performance Liquid Chromatography)
  • Method for rapidly measuring content of solanesol in tobaccos by using UPLC (Ultra Performance Liquid Chromatography)

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Rapid determination of solanesol content in different types of tobacco samples

[0026] 1. Measuring instruments and implementation conditions:

[0027] 1.1 Chromatographic equipment and conditions: ACQUITYUPLC H-CLASS ultra-high performance liquid chromatography-diode array detector (PDA), equipped with Empower 2 chromatography workstation, UPLC-XEVO Q-TOF, American Waters Company, equipped with MassLynx 4.1 mass spectrometry software, column model : BEH C18 (1.7μm, 2.1mm×50mm), manufactured by Waters Corporation of the United States, the mobile phase of the chromatographic column is 100% methanol for isocratic elution, the flow rate is 0.4mL / min, the column temperature is 30°C, the injection volume: 2μL, and the detection The wavelength is 207nm, and the running time is 5min.

[0028]1.2 Mass spectrometry conditions: Atmospheric pressure chemical ionization source (APCI), positive ion mode detection, corona current 4μA, corona needle temperature 500°C, sampling cone ...

Embodiment 2

[0047] Method repeatability, recovery and stability determination

[0048] 1. Method repeatability and recovery

[0049] Weigh 10 samples of 50.0000 mg of tobacco powder of the same first-cured tobacco leaves, and divide them into two groups, 5 samples in each group. One group was measured in parallel 5 times according to the experimental conditions of 1.1 and 2.1, and the repeatability of the method was calculated. The results are shown in Table 2. Another group of samples was added with 0.68 mg of solanesol standard substance, and the recovery rate was calculated according to the amount of the standard sample added and the measured amount of the sample before and after the addition of the standard. The results are shown in Table 3.

[0050] The results showed that the precision determination RSD% of solanesol was 2.62%, and the recovery rate was 91.1%.

[0051] The repeatability experiment result (n=5) of table 2 method

[0052] sample number Sample weight (g)...

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Abstract

The invention discloses a method for measuring the content of solanesol in tobaccos by using a UPLC (Ultra Performance Liquid Chromatography). The method comprises the following steps of: grinding the tobaccos into powder and weighing 50 mg of the tobacco powder; putting the weighed tobacco powder into a centrifuge tube with the capacity of 50 mL and adding 25 mL of a methanol solution; placing the centrifuge tube into an ultrasonic vibrator to be ultrasonically extracted for 10 min; taking 2 mL of an extracting solution and filtering the extracting solution by a filter membrane with the size of 0.22 micron to obtain clean liquid; and utilizing the ultra performance liquid chromatography to analyze to detect the content of the solanesol in the tobaccos. The method disclosed by the invention has the advantages of simple extraction method, convenience for detection operation, high flexibility and good repeatability, thereby rapidly and accurately detecting the content of the solanesol in tobacco samples to be detected.

Description

technical field [0001] The invention relates to a method for quickly measuring the content of solanesol in tobacco leaves by using UPLC technology, and belongs to the technical field of liquid chromatography analysis. Background technique [0002] Solanesol is an acyclic tetrasesquiterpene alcohol. It was first extracted from flue-cured tobacco in 1956 and its structure was identified. Solanesol is a natural compound that widely exists in mammals, higher plants and microorganisms, especially in tobacco with the highest content. Solanesol plays an important role in biochemistry. It has a very strong ability to absorb free radicals. It can play antibacterial, anti-inflammatory, hemostatic and anti-ulcer effects and strong anti-cancer activity. Therefore, it is an important raw material for drug synthesis and a pan- It is an irreplaceable key intermediate of quinone drugs (such as coenzyme Q10, vitamin K2 and anticancer synergists, etc.), and has extremely important value in p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 耿召良张婕汪云松向章敏蔡凯周淑平许冬青
Owner GUIZHOU TOBACCO SCI INST
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