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A technology for heavy metals and plants, applied in the field of detection, can solve the problems of not being able to indicate the existence state, the effectiveness of migratory plant absorption, and the inability to be used as a sufficient standard for assessing biological effects.
Inactive Publication Date: 2013-04-03
DASHUN INT FLOWER
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[0004] After heavy metals enter the soil, they will cause permanent potential harm to plants, animals, and microorganisms, and the analysis of the total amount of heavy metals in the soil can give information about the possible enrichment of heavy metals in the soil, but it cannot indicate the existence and migration of the elements in the soil. capacity and availability for uptake by plants are not sufficient criteria for assessing their impact on organisms
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Embodiment 1
[0018] (1) Take 2g of the crushed and air-dried chrysanthemum chrysanthemum sample and put it into a conical flask, add water to moisten it, add 25mL of nitric acid and 10mL of perchloric acid, let it stand for a day, then heat it to produce white smoke, after cooling, add 2mL of hydrochloric acid, add water to set Make up to 50mL, centrifuge, and take the supernatant;
[0019] (2) Put the supernatant into a 150ml separating funnel, add 2.0mL of ascorbic acid solution, 2.5mL of potassium iodide solution, shake well, then add 5.00mL of methyl isobutyl ketone accurately, shake for 1-2min, let stand to separate layer, take the organic phase for testing;
[0020] (3) Preparation of standard curves for cadmium and lead heavy metals;
[0021] (4) On-machine detection, the heavy metal content was calculated according to the standard curve, the cadmium content in Chrysanthemum chrysanthemum was 0.39mg / kg, and the lead content was 0.26mg / kg.
Embodiment 2
[0023] (1) Put 2 g of crushed and air-dried leek samples into a conical flask, add water to moisten them, add 25 mL of nitric acid, 10 mL of perchloric acid, let stand for a day, then heat until white smoke is produced, after cooling, add 2 mL of hydrochloric acid, add water to set Make up to 50mL, centrifuge, and take the supernatant;
[0024] (2) Put the supernatant into a 150ml separating funnel, add 2.0mL of ascorbic acid solution, 2.5mL of potassium iodide solution, shake well, then add 5.00mL of methyl isobutyl ketone accurately, shake for 1-2min, let stand to separate layer, take the organic phase for testing;
[0025] (3) Preparation of standard curves for cadmium and lead heavy metals;
[0026] (4) On-machine detection, calculate the heavy metal content according to the standard curve, the cadmium content in leek is 0.23mg / kg, and the lead content is 0.38mg / kg.
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Abstract
The invention discloses a method for detecting heavy metal in plants. The method comprises the following steps of (1) placing 2g of crashed and air-dried plant samples into a conical flask; adding water to moist; adding 25ml of nitric acid and 10mL of perchloric acid; standing for one day, and then heating until the plant samples generate white smoke; adding 2mL of hydrochloric acid after the plant samples are cooled; adding water until the constant volume is 50mL; and centrifuging and taking supernate; (2) placing the supernate into a separating funnel with a volume of 150ml; adding 2.0mL of an ascorbic acid resolution and 2.5mL of a potassium iodide solution; uniformly shaking and then accurately adding 5.00mL of methyl isobuthyl ketone; shaking for 1-2 minutes and standing for layering; and taking an organic phase for future measurement; (3) preparing a heavy metal standard curve; and (4) detecting by using a computer, and calculating a content of the heavy metal according to a standard curve. The method provided by the invention has the advantage of rapidly and accurately detecting the content of the heavy metal in the plants.
Description
technical field [0001] The invention belongs to the detection field, in particular to a method for detecting heavy metal content in plants. Background technique [0002] Lead and cadmium are important polluting heavy metal elements, as well as non-essential elements for humans, animals, and plants. The research on soil lead pollution has attracted more and more attention from scholars at home and abroad. With the continuous increase of agricultural utilization of urban waste, the trend of heavy metal pollution in farmland is becoming more and more obvious, and the situation of lead pollution is also becoming more and more serious. Lead pollution can hinder the growth of crops, greatly reduce the yield and quality. Studies have shown that the correlation between the total amount of soil lead and crop yield and lead absorption is lower than that of soil available lead, that is, available lead is more representative of the bioavailability of lead. Therefore, it can be said t...
Claims
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