105results about How to "Improve sensitivity and accuracy" patented technology

Apparatus and methods for performing microanalysis of particles using a microelectrical-mechanical system (MEMS) chip to electrically interrogate the particles. The MEMS chip is typically manufactured using known lithographic micromachining techniques, employed for example, in the semiconductor industry. A substrate carries a plurality of microelectrodes disposed in a detection zone and spaced apart along an axis of a microchannel. The microchannel is sized in cross-section to cause particles carried by a fluid to move past the electrodes in single file. Impedance is measured between one or more pairs of electrodes to determine the presence of a particle in the detection zone. In certain embodiments used in cell manipulation, an electroporation signal may be applied between one or more pairs of electrodes to enhance permeability of a cell membrane. A structural arrangement may be provided to introduce a treatment substance into the microchannel in the vicinity of a cell, which may be restrained for a period of time in a treatment zone.

Instruments and methods for measuring a property of a shape memory alloy are provided. The instrument includes a base plate, a non-contact movable mass, a force gauge, an actuator, a biasing spring, a heater for heating the shape memory alloy, and a non-contact displacement detector. The biasing spring and the shape memory alloy are disposed whereby a force applied thereby is applied substantially through a center of stiffness of the movable mass. The displacement detector measures a displacement of the movable mass in a colinear direction with a direction of movement of the movable mass and with a direction of the force applied by the biasing spring and the shape memory alloy. The linear motion stage comprises a housing and at least one guide bar, and wherein a calculated axial expansion of the guide bar is substantially equal to a calculated axial expansion of the base plate.

The present invention relates to test devices, and in particular devices capable of detecting the presence or absence of an analyte in a sample, such as a liquid sample. Also provided are methods of using such devices for quantitative or qualitative measurement of one or more analytes in a liquid sample.

An imaging system with an imaging mechanism which includes polarization analyzers, which may be crossed polarization analyzers, positioned to provide birefringence images of particles in the fluid passing through the flow chamber. Captured images are of high resolution and may be used in comparison to known images of a library of images. The system and related method enhance the accuracy and sensitivity of particle monitoring by utilizing birefringence imaging combined with particle analysis and the detection of each particle's characteristic features, such as crystalline features. The system includes a scatter detector used to trigger backlighting of the flow chamber and capture images of particles therein.

The invention relates to the technical field of robots, in particular to a robot collision detection method and device, a storage medium and a robot. The method comprises the steps of acquiring the first motor torque, the first angular displacement, the first angular velocity and the first angular acceleration of a robot joint; updating kinetic parameters of the robot in a preset updating mode andcalculating the theoretical torque of the joint according to the updated kinetic parameters, the first angular displacement, the first angular velocity and the first angular acceleration; obtaining the first external torque of the joint according to the first motor torque and the theoretical torque; inputting the first motor torque, the first angular displacement, the first angular velocity and the first angular acceleration into a preset observer model to obtain the second external torque of the joint; judging whether the first external torque is greater than a first preset threshold value or not and judging whether the second external torque is greater than a second preset threshold value or not; and determining that collision occurs to the robot if the first external torque is greaterthan the first preset threshold value and the second external torque is greater than the second preset threshold value.

The invention relates to a device for analyzing an analyte in a liquid sample. The device can be used for acquiring the liquid sample and simultaneously detecting whether the analyte exists in the sample or not. The device comprises a collection chamber for storing the liquid sample, a detection chamber for accommodating a detection element and a liquid sample transfer chamber, wherein the transfer chamber is connected with the detection chamber and the collection chamber, and the detection chamber is not in direct liquid communication with the liquid sample collection chamber; the transfer chamber is provided with a first position and a second position; when the transfer chamber is positioned in the first position, the transfer chamber is opened to the collection chamber and closed to the detection chamber and can receive a part of liquid sample in the collection chamber to the transfer chamber; and when the transfer chamber is positioned in the second position, the transfer chamber is closed to the collection chamber and opened to the detection chamber and can release the liquid sample in the transfer chamber to the detection chamber. The liquid sample is transferred to the detection chamber from the collection chamber through the transfer chamber so as to carry out detection or test, therefore, the pollution to the liquid sample in the collection chamber from the detection element in the detection chamber is avoided.

A particle detection system with a detection mechanism that includes detectors positioned to detect two different ranges of fluorescence produced by particles in the fluid in a flow chamber. Each of the detectors is arranged to generating a trigger signal whenever fluorescence is detected. The system and related method enhance the accuracy and sensitivity of blue-green algae monitoring by utilizing imaging flow cytometry combined with particle analysis and the measurement of the ratio of each particle's phycocyanin to chlorophyll b detected by using the two detectors configured for detection of two different fluorescence ranges, one associated with the phycocyanin and the other associated with the chlorophyll b. Optionally, captured images may be used in comparison to known images of a library of images.

The invention discloses a multi-sample multi-site SNP (single nucleotide polymorphism) detection method, and belongs to microarray chip-based SNP multi-sample multi-site detection technology. The method comprises the following steps of: designing an oligonucleotide DNA chip; capturing different SNP sites of the same sample by using different Padlock probes in a single tube, then hybridizing specific amplification primers and the Padlock probes and performing rolling circle amplification, and finally fixing different SNP amplification products of different samples on the same chip in certain order to prepare a multi-sample multi-site SNP microarray to be detected; and finally, performing detection by using a general fluorescent probe, and judging the parting of the SNP according to the color of microarray point fluorescence. The method has the advantage of realizing parallel detection of different SNP sites of different samples and detection of different SNP sites of the same sample. Accuracy and sensitivity of detecting SNP site information are high. Because of the specificity and the accuracy of identifying the SNP sites by the Padlock probes, the accuracy of identifying the SNP sites is ensured.

The invention discloses a kit for detecting a related immunophenotyping of B-cell acute lymphoblastic leukemia and forms classification equipment of a related immunophenotyping of leukemia with a seven-color flow cytometry. The kit is configured with the following monoclonal antibody reagent combinations: CD58, CD10, CD34, CD123, CD38, CD19 and CD45, and fluorescein is respectively marked according to front and back sequences as follows: FITC (fluorescein isothiocyanate), PE (pulmonary embolism), PerCP-CY5,5, PE-CY7 (phycoerythrin-CY7), APC (adenomatous polyposis coli), APC-Cy7 and PacificBlue. With the adoption of the kit disclosed by the invention, whether the seven marks are normal or not can be simultaneously observed, and the sensitivity and the accuracy of the detection are increased; further, immunophenotyping information in morbidity can not be relied on, so that the range and capacity of the LAIP (leukemia associated immunophenotyping) analysis are expanded, the early-stage conditions of the analysis are simplified, and more chances are provided for clinical detection of MRD (minimal residual disease).

The invention discloses a method for measuring focal plane uniformity of an exposure machine, comprising: manufacturing a special mask of which a figure zone has a plurality of same table cell, wherein each table cell is provided with a plurality of typical figures; adopting the special mask, and utilizing an exposure machine to respectively expose different zones of one wafer; utilizing a defectdetection machine to automatically detect the defect condition of the exposed wafer which is used for measuring; according to the defect condition, combining the translational amount of a focal distance to calculate and obtain the focal plane uniformity of the exposure machine. The invention improves the sensitivity and the accuracy for measuring the focal plane uniformity of the exposure machine, improves the measuring efficiency and is favourable for maintaining the focal plane of the exposure machine and monitoring performance.

The invention discloses an ARMS (Amplification Refractory Mutation System) primer for detecting human EGFR, KRAS and BRAF gene mutation. The ARMS primer is characterized in that sequences of the ARMS primer are as shown as in SEQ ID NO.1 to SEQ ID NO.31. The invention also discloses a kit containing the ARMS primer. According to the ARMS primer disclosed by the invention, a double-internal reference system is guided in an ARMS real-time fluorescence quantification technology, a normal site reference which is consistent with a mutation site is added for contrast expect a common internal reference, the defects of the prior art are avoided by the double-internal reference system and a corresponding double interpretation rule, the detecting accuracy is increased, and the error rate is reduced; a reagent which is used for carrying out Sanger sequencing on a sample is also contained in the detecting kit; by combining a Sanger sequencing technology with the ARMS real-time fluorescence quantification technology, a mutation site of a known gene can be detected, a new mutation site can also be found, and the detecting sensitivity is ensured.

The invention relates to a human kallikrein-1 of mammalian cell expression, as well as a coding gene, an expressing method, a purifying process, a recombinant protein detecting method and application thereof. Kallikrein is serine protease, and plays a very important physiological role in human tissue. A conventional tissue extracting method has the problems of higher immunogenicity, or difficulty in source, insurmountability of virus contamination, and the like, and a common human kallikrein-1 of mammalian cell expression also has the defect of low expression quantity or lower activity. Therefore, the invention provides a coding gene for a recombinant hK1 as shown in SEQ ID NO:1, as well as a special expression, purification, recombinant protein detection method and application for the coding gene. Compared with the prior art, the recombinant hK1 provided by the invention is higher in expression quantity and activity; the purification process established by the invention and a drug detection method of the recombinant human kallikrein-1 lays the foundation in cell line development, process optimization, preclinical and clinical research and industrialized production in the drug development process.

The invention provides a temperature controller of the power battery of an electric car. The temperature controller is arranged nearby a battery, and comprises a plurality of temperature probes, a microprocessor and a heating device, wherein the temperature probes and the heating device are mutually and electrically connected with the microprocessor and the battery respectively; when the current battery temperature detected by any one temperature probe is lower than the floor temperature, the microprocessor gives out a heating control instruction, so that the heating device is started to heat the battery; and when the current battery temperature detected by any one temperature probe is higher than the ceiling temperature, the microprocessor gives out a heating-stopping control instruction, so that heating of the heating device on the battery is stopped. Through detecting the battery temperature on multiple points, the real-time monitoring of the multi-position temperature of the battery is achieved, the detecting accuracy and the detecting sensitivity are improved, the battery can be heated automatically in a low-temperature environment, and the heated battery keeps higher volume in a cold environment to improve the use efficiency of the battery effectively.

The invention relates to a high performance liquid chromatography based illegal cooking oil cluster analysis method. Main components of a target oil sample comprise triglyceride and other impurities, and the analytic detection of illegal cooking oil is realized through the cluster determination of high performance liquid chromatography peaks. The method is mainly characterized in that a high performance liquid chromatography technology is adopted and fourteen characteristic chromatographic peaks are selected to obtain the area percentages of the peaks, and the area percentages are normalized; a cluster analysis technology is adopted to cluster the known oil sample to six types and establish a sample database; and data of an unknown sample are detected through discriminant analysis. The detection method has the advantages of simple sample pretreatment, high accuracy, and good repeatability, and can effectively avoid the false negativity appearing in the single impurity component detection because a plurality of fingerprints are adopted for identifying.

The invention relates to a method for analyzing a target nucleic acid fragment, comprising generating a first strand using one strand of the target as a template by primer extension, using a first oligonucleotide primer which comprises, from 5′ to 3′, an overhang adaptor region, a primer ID region and a target specific sequence region complementary to one end of the target fragment; optionally removing non-incorporated primers; amplifying the target from the generated first strand to produce an amplification product; and detecting the amplification product. Also disclosed are unique primers useful for such target analysis methods.

The invention provides a vehicle-mounted altitude monitoring device and a vehicle-mounted altitude monitoring method. The device comprises a central control module, a sensor module connected to the central control module, a power supply module for supplying power to the device and a display module; the sensor module acquires an air pressure signal and a temperature signal of a position where the device is positioned, and inputs the signals to the central control module; the central control module performs temperature compensation on the temperature signal and performs filtering processing on the air pressure signal respectively to calculate a relative altitude height value of the position where a vehicle is positioned; and the value is displayed by the display module. The vehicle-mounted altitude monitoring method is high in accuracy and sensitivity, and the vehicle-mounted altitude monitoring device is intelligent, and simple in structure, low in cost and small in volume.

A micromechanical biochemical transducer of nanostructure is prepared as setting micro suspension ¿C arm on SOI silicon base, coating silica insulation layer on said arm with its top layer being heating resistance prepared by single ¿C crystal silicon, coating nanomaterial on heating resistance, connecting nanomaterial heating unit to heating resistance by leading wire, setting laser generator and transducer output signal detection unit of position sensitive detector above micro suspension ¿C arm.

The invention discloses a natural disaster early warning system and method, a cloud platform and a storable medium. The method comprises the steps: acquiring the abnormal environment identification data corresponding to the geological disaster monitoring equipment, and grouping the geological disaster monitoring equipment according to the preset grouping strategy for the geological disaster monitoring equipment; migrating the abnormal environment identification data corresponding to the geological disaster monitoring equipment of the same category to the same data set, acquiring the first disaster environment state information of the category and the second disaster environment state information of each abnormal environment identification data in the data set corresponding to the category, carrying out the fusion analysis on the obtained different disaster environment state information, so the abnormal environment change early warning of the geological disaster early warning area can be realized based on the geological disaster monitoring equipment in the associated category; the problem that individual geological disaster monitoring equipment is easily interfered by external factors is avoided through the geological disaster monitoring equipment in the associated category, and the accuracy of abnormal environment change early warning and the disaster detection sensitivity of the abnormal environment change content can be improved.

The invention relates to a method for detecting PAEs with molecular beacon fluorescence quantitative PCR. The method comprises the following steps that: cytosol which is extracted from carp liver and contains a receptor is combined with PAEs of different concentration to form compounds; the compounds are combined with bound DNA synthesized by specific primers to form ligand-receptor-DNA compounds; the ligand-receptor-DNA compounds are digested by exonuclease III and S1 nuclease; the products of the enzyme digestion are used as templates for molecular beacon fluorescence quantitative PCR amplification, and a regression equation between concentration of added PAEs and copy numbers of an initial template is obtained; according to the regression equation and a quantitative DNA standard curve,the relationship between the concentration of PAEs and Ct values is obtained through linear regression analysis, thereby enabling detection of PAEs of different concentration to be realized. The method provided in the invention has the characteristics of high sensitivity, low detection limit and strong specificity, is applicable to rapid detection of the content of PAEs in mass samples, and has agood application prospect.

An optical switch keyboard includes a circuit module and plural keys. The plural keys are disposed over the circuit module. The circuit module includes a circuit board, plural light emitters and plural light receivers. Each key is disposed on a top surface of the circuit board. Each key corresponds to a light emitter and a light receiver. The plural light emitters and the plural light receivers are disposed on a bottom surface of the circuit board. Consequently, the plural light emitters and the plural light receivers are not interfered by the ambient light that comes from the top side of the circuit board. In addition, the optical switch keyboard has waterproof structures to prevent foreign liquid from entering the optical switch keyboard.

The invention relates to a method for detecting nonyl phenol by exonclease protection fluorescent quantitative PCR. The method comprises the following steps: combining the characteristic primer with DNA, nonyl phenol receptor extracted from fish liver and nonyl phenol with different concentrations to form a composite; ensuring the dissociative DNA not to be augmented by resolving the compound by nucleic acid exonclease III and S1 nucleic acid exonclease; taking the product after enzyme cutting as a template, and carrying out fluorescent quantitative PCR augmentation to obtain a regression equation adding nonyl phenol concentration and copy number of an initial template; utilizing quantitative DNA standard curve and linear regression analysis to obtain the relationship between nonyl phenol concentration and a Ct value; and carrying out the detection on the nonyl phenol with different concentrations. The invention has the characteristics of high sensitivity and low detection limit, and can be used for rapidly detecting the content of the nonyl phenol in a large quantity of samples.

The present invention provides a portable biometric wrist device (10) having an optical sensor and comprising at least one light source (41) and at least one light detector (40) installed onto a circuit board (33), a casing (20) having at least one aperture (43) for said at least one light source (41) and at least one aperture (42) for said at least one light detector (40), wherein each of said at least one light source (41) and at least one light detector (40) are optically isolated from each other inside said casing (20) with a sealing plate (32) between said casing (20) and said circuit board (33), said sealing plate (32) having at least one aperture for said at least one light source (41) and at least one aperture for at said least one light detector (40). The present invention also provides a method for manufacturing said portable biometric wrist device (10).

The invention belongs to the field of molecular biology and biomedicine, and particularly relates to gastric cancer prognosis model construction based on an m6A-related IncRNA network and clinical application. According to the invention, a relationship between m6A-related lncRNA and a total survival rate of the gastric cancer is analyzed, 11 m6A related lncRNA markers are found, and a prognosis model is constructed to predict the prognosis of the gastric cancer. The model for predicting the prognosis of the gastric cancer contains 11 IncRNAs related to the prognosis of the gastric cancer. Theinvention provides the prediction model constructed on the basis of the m6A-related IncRNA network to predict the prognosis of a gastric cancer patient; the feasibility of the model is verified in multiple data sets; and a reliable method is provided for analyzing the prognosis of the gastric cancer related LncRNA patient. The model provides a plurality of lncRNAs as biomarkers, the sensitivity and accuracy of predicting the prognosis of the gastric cancer are improved, the adverse prognosis risk of the gastric cancer patient is accurately predicted and evaluated, and key monitoring and effective intervention are performed on a high-risk patient, so that a death rate and a local recurrence rate of the gastric cancer patient are reduced, and the prognosis of the patient is improved.

The invention relates to a method for detecting alkaline phosphatase in body fluid. The method comprises the following steps: triphosadenine (ATP) is used as a catalytic substrate of alkaline phosphatase (ALP), wherein triphosadenine is converted into adenosine in the presence of the catalysis of alkaline phosphatase; and luciferase can be used for catalyzing fluorescein to emit light under the condition that energy is provided by triphosadenine, and the light emitting intensity is in direct proportion to the content of ATP. When the quantity of ALP in a sample is more, more ATP is consumed so that the change amount of biological light emission is greater. Therefore, the content of alkaline phosphatase can be calculated by measuring the change amount of the light emitting intensity of the biological light emission. With the adoption of the method, 10-15M alkaline phosphatase can be detected; compared with the prior art, the method has the advantages that the sensitivity is high, the anti-jamming capability is strong, the operation is simple, and a detection instrument is easy to carry and is suitable for an analysis method of clinical diagnosis, and besides the advantages, the detection time can be greatly shortened and the detection cost is reduced.

Disclosed is a method for improving the sensitivity and accuracy of quantitative detection of polynucleotides in a sample, such a clinical specimen, by a method that utilizes a two- or three-step process of tagging/labeling target molecules and adding an adapter sequence for adding a universal primer for efficient amplification of targets while decreasing target amplification bias. When combined with the step of statistically correcting for sequencing errors, the method can significantly increase the accuracy of quantitative detection of polynucleotides in a sample.

The invention belongs to the field of analytic chemistry and relates to a low-concentration retapamulin determination method and an application, in particular to a method for determining low-concentration retapamulin with high-performance liquid chromatography-tandem mass spectrometry as well as an application. According to the method, the high-performance liquid chromatography-tandem mass spectrometry is adopted for determination, the mass spectrum adopts a multi-reaction monitoring mode, further, an internal standard method is adopted, and tiamulin is taken as an internal standard substance. The determination method has the advantages of high specificity, sensitivity and accuracy and good repeatability, the limit of detection can be up to 200 pg/ml, the limit of quantitation can be up to 500 pg/ml, and the determination method can be widely applied to concentration tests of low-concentration retapamulin biological samples, especially sample concentration testing of retapamulin ointment transdermal tests, and has great significance for retapamulin quality control.

The invention relates to a method for rapidly identifying true and false pesticides based on portable differential Raman technology. The method adopts a portable differential Raman system, uses two wavelength light sources to detect samples, and comprises the following steps of firstly collecting two pieces of Raman spectrogram data of commercially available pesticides detected by a double-light source Raman spectrometer with stable and tiny wavelength difference, differentiating the two Raman spectra, and performing special algorithm filtering on Raman characteristic signals generated by thedifferentiation to separate the differential signals from baseline deviation, thereby obtaining the differential characteristics of the pesticide samples with eliminated interference of fluorescence and the like; and finally comparing the differential characteristics with the spectrogram characteristic peaks of pesticide standard samples to realize the identification of true and false of commercially available pesticide components. By adopting the double-light source excited differential technology, the sample background interference is eliminated, and more detailed Raman characteristics of the sample are accurately extracted by performing characteristic filtering realization on the differential spectrogram; and a characteristic database and classification identification of the commercially available pesticides are established for the differential Raman characteristics. By using the differential technology, the accuracy and the sensitivity of the detection are greatly improved, the portable Raman system for the detection on the commercially available pesticides is realized, and the detection can be completed without any pretreatment process; and the method is simple in operation, fast in detection speed, high in accuracy and has significance for the pesticide quality safety control.

The invention relates to a device and technological method for detecting the distillation range of crude oil through a mass method. The device is characterized in that a distillation flask is connected with a temperature tester, a branch pipe of the distillation flask is connected with a condensation pipe, and a condenser is connected to the condensation pipe; the outlet end of the condensation pipe is opposite to a receiver which is placed on an electronic scale. The detection method includes the steps that 100 g of crude oil samples are weighed and placed into the distillation flask; secondly, the distillation flask containing the crude oil samples is heated, the distillation temperature of the crude oil samples is accurately measured through the temperature tester, and the mass of distilled substances is continuously weighed through the electronic scale; thirdly, temperature which is continuously measured by the temperature tester and data of continuous weighing, performed by the electronic scale, of the distilled substances are calculated and processed, and an analysis data result of the distillation range of crude oil is obtained. According to the device and technological method for detecting the distillation range of crude oil through the mass method, the distillation range of crude oil is analyzed in the mode that the crude oil samples and the distilled substances are weighed through the mass method, and therefore analysis of the distillation range of crude oil can be more accurate and faster.