Method for detecting nonyl phenol by exonclease protection fluorescent quantitative PCR
A fluorescence quantification and nonylphenol technology, which is applied in the direction of fluorescence/phosphorescence, microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problems of low sensitivity and high false positive rate, and achieve high sensitivity and detection limit Low, the effect of improving accuracy and sensitivity
Inactive Publication Date: 2010-04-07
DONGHUA UNIV
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Abstract
The invention relates to a method for detecting nonyl phenol by exonclease protection fluorescent quantitative PCR. The method comprises the following steps: combining the characteristic primer with DNA, nonyl phenol receptor extracted from fish liver and nonyl phenol with different concentrations to form a composite; ensuring the dissociative DNA not to be augmented by resolving the compound by nucleic acid exonclease III and S1 nucleic acid exonclease; taking the product after enzyme cutting as a template, and carrying out fluorescent quantitative PCR augmentation to obtain a regression equation adding nonyl phenol concentration and copy number of an initial template; utilizing quantitative DNA standard curve and linear regression analysis to obtain the relationship between nonyl phenol concentration and a Ct value; and carrying out the detection on the nonyl phenol with different concentrations. The invention has the characteristics of high sensitivity and low detection limit, and can be used for rapidly detecting the content of the nonyl phenol in a large quantity of samples.
Application Domain
Microbiological testing/measurementFluorescence/phosphorescence
Technology Topic
S1 nucleaseFish liver +8
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