Kit for detecting related immunophenotyping of B-cell acute lymphoblastic leukemia and application thereof

A technology for B lymphocytes and related immunity, applied in the field of kits for detecting acute B lymphocytic leukemia-related immune phenotypes, can solve the problem of increasing the test cost and the economic burden of patients, affecting the multi-parameter analysis of detection antibodies, and being unable to detect antibodies at the same time, etc. problems, to achieve the effect of increasing sensitivity and accuracy, facilitating standardization and normalization, and reducing test costs
CN103018463AActive Publication Date: 2013-04-03PEOPLES HOSPITAL PEKING UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
PEOPLES HOSPITAL PEKING UNIV
Publication Date
2013-04-03

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Abstract

The invention discloses a kit for detecting a related immunophenotyping of B-cell acute lymphoblastic leukemia and forms classification equipment of a related immunophenotyping of leukemia with a seven-color flow cytometry. The kit is configured with the following monoclonal antibody reagent combinations: CD58, CD10, CD34, CD123, CD38, CD19 and CD45, and fluorescein is respectively marked according to front and back sequences as follows: FITC (fluorescein isothiocyanate), PE (pulmonary embolism), PerCP-CY5,5, PE-CY7 (phycoerythrin-CY7), APC (adenomatous polyposis coli), APC-Cy7 and PacificBlue. With the adoption of the kit disclosed by the invention, whether the seven marks are normal or not can be simultaneously observed, and the sensitivity and the accuracy of the detection are increased; further, immunophenotyping information in morbidity can not be relied on, so that the range and capacity of the LAIP (leukemia associated immunophenotyping) analysis are expanded, the early-stage conditions of the analysis are simplified, and more chances are provided for clinical detection of MRD (minimal residual disease).
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Description

technical field

[0001] The invention belongs to a special kit in the process of confirming cell phenotypes, and in particular relates to a kit for detecting immunophenotypes related to acute B lymphocytic leukemia and its application. Background technique

[0002] With the improvement of treatment strategies and the continuous emergence of new chemotherapy drugs, the remission rate and survival rate of acute lymphoblastic leukemia (ALL) have been greatly improved, but there are still some patients with poor prognosis and eventually die of relapse. Even with intensive chemotherapy or even hematopoietic stem cell transplantation after relapse, it is still difficult to save the patient's life, which has caused extremely heavy mental and economic burdens to society and families. Studies have proven that the level of minimal residual disease (MRD) after treatment is the root cause of relapse.

[0003] Multi-parameter flow cytometry (flow cytometry, FCM) is one of the commonly ...

Claims

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