Detection method for tannin in mulberry leaf

A detection method and medium tannin technology are applied in the field of detection of tannin in mulberry leaves, which can solve the problem that there is no rapid method for measuring tannin content in mulberry leaves, and achieve the effects of high accuracy and simple operation.

Inactive Publication Date: 2017-04-26
CHONGQING ACAD OF ANIMAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no research report on the method of rapid determination of tannin content in mulberry leaves

Method used

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  • Detection method for tannin in mulberry leaf
  • Detection method for tannin in mulberry leaf
  • Detection method for tannin in mulberry leaf

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The drawing of embodiment 1 tannin standard curve

[0032] (1) Preparation of tannin standard solution

[0033] Accurately weigh 0.2000g of tannin standard substance, dissolve it with 10% methanol solution, and adjust the volume to 100mL to prepare 100mL of tannin standard solution with a concentration of 2.0mg / mL.

[0034] (2) Drawing of standard curve

[0035] Pipette 1 mL, 2 mL, 3 mL, and 4 mL of the tannin standard solutions prepared in step (1) into 100 mL volumetric flasks, and dilute to volume with methanol. The tannin concentrations are 20 μg / mL, 40 μg / mL, 60 μg / mL, and 80 μg / mL , respectively take 10 μL of each concentration solution for injection analysis, the chromatogram of the tannin standard is as follows figure 1 As shown, the concentration is 60μg / mL and the chromatogram of individual injection detection is as follows figure 2 As shown, the chromatographic column used is Merck Hibar, LiChrosorb RP 18, the specification is 250mm×4.6mm id, 5μ (Art.5033...

Embodiment 2

[0047] Example 2 sample detection

[0048] Weigh 0.5g (accurate to 0.0001g) of mulberry leaf powder dried to constant weight, dissolve 100mL with 10% methanol solution, shake on a shaker for 40min, and filter with a 0.45μm filter membrane to obtain a sample extract. Get 10 μ L sample injection of sample extract, detect according to the chromatographic condition of embodiment 1, the obtained chromatogram is as follows Figure 4 As shown, read its peak area, as shown in Table 4, obtain the tannin content of sample according to the standard curve of embodiment 1 and be 0.81%.

[0049] Table 4 Peak area table

[0050]

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Abstract

The present invention relates to the technical field of plant effective component analysis, more particularly to a method for detecting tannin in mulberry leaf. The method specifically comprises: (1) taking a tannin standard substance, dissolving with a dilution liquid to prepare a tannin standard solution with a known concentration, carrying out injection analysis according to the tannin standard solution, and performing linear regression by corresponding the peak area to the tannin concentration to obtain a standard curve; and (2) taking a mulberry leaf sample, dissolving with a dilution liquid, filtering to obtain a mulberry leaf extract, carrying out injection analysis to obtain the peak area of the mulberry leaf extract; and obtaining the tannin content in the mulberry leaf sample according to the standard curve obtained in the step (1). According to the present invention, the method has advantages of simple operation and high precision, and can rapidly detect the tannin content in the mulberry leaf sample.

Description

technical field [0001] The invention relates to the technical field of plant active ingredient analysis, in particular to a method for detecting tannins in mulberry leaves. Background technique [0002] Tannin is a highly polymerized compound among polyphenols. It contains many phenolic hydroxyl structures and has a relative molecular weight of 500-4000. It can undergo complex reactions with proteins, alkaloids, polysaccharides and various metal ions. It has been widely used in medicine and food , tanning, daily chemicals, water treatment and other fields. Tannin has strong chemical and physiological activity, and has dual effects on organisms. Excessive tannin in food will produce anti-nutritional effects such as reducing the effective absorption of protein and vitamins. [0003] Mulberry leaves are the main product of mulberry trees, accounting for about 64% of the above-ground production. In 1993, the Ministry of Health listed mulberry leaves as medicinal and edible plan...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 王介平曾秀周婵李玉婷王海燕吕金凤
Owner CHONGQING ACAD OF ANIMAL SCI
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