Adapter sensor and manufacturing method thereof

An aptamer sensor and sensor technology, which is applied in instruments, scientific instruments, ion implantation plating, etc., can solve the problems of difficult antibody preparation, expensive antibodies, and time-consuming

Inactive Publication Date: 2019-06-18
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Production of high-affinity antibodies is expensive and time-consuming
Therefore, it is not easy to prepare antibodies against any target molecule

Method used

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  • Adapter sensor and manufacturing method thereof
  • Adapter sensor and manufacturing method thereof
  • Adapter sensor and manufacturing method thereof

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Embodiment Construction

[0022] In the following, the technical solutions in the embodiments of the present invention will be described in detail with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0023] It should be noted that, in this document, the terms "comprising", "comprising" or any other variation thereof are intended to cover a non-exclusive inclusion such that a process, method, article or apparatus comprising a set of elements includes not only those elements, It also includes other elements not expressly listed, or elements inherent in the process, method, article, or apparatus.

[0024] 1 Materials and methods

[0025] 1.1 Materia...

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Abstract

The invention relates to a Listeria monocytogenes rapid ultra-sensitive detector which is based on nanochannel confinement properties and constructed through utilizing properties of specific recognition between a target molecule and its aptamer and further relates to a self-made electrolytic cell through taking a porous alumina film modified with a Listeria monocytogenes DNA aptamer as an electrode; potassium ferricyanide ions act as probe ions, and Listeria monocytogenes are detected. When the LM is present and the concentration is low, a current rising value decreases significantly with theincreasing concentration; as the concentration of the LM increases, a current change value decreases; when the LM concentration is in the range of 100-1250 CFU / mL, the linear relationship with the current rising value is realized; when the concentration is greater than 1500 CFU / mL, the current change value tends to be stable. Therefore, the detection limit for the LM can reach 100 CFU / mL, the linear range is 100-1250 CFU / mL, and detection can be completed in 10 minutes. A 108 CFU / mL E. coli and S. aureus control experiment showed that high selectivity for Listeria is realized.

Description

[0001] This application is a divisional application with application number 201610333218.8, application date of May 19, 2016, and the title of the invention is "New Method for Highly Sensitive Detection of Listeria Based on Aptamer-Modified Porous Alumina Membrane". technical field [0002] The invention relates to an ultrasensitive detector for Listeria monocytogenes based on the confinement property of a nanochannel constructed by using the property of specific recognition between a target molecule and its aptamer. The present invention also relates to the porous aluminum oxide film modified by the DNA aptamer of Listeria monocytogenes as an electrode to assemble a self-made electrolytic cell; the potassium ferricyanide ion is used as a probe ion to rapidly and efficiently treat Listeria monocytogenes. method for sensitive detection. Background technique [0003] Listeria monocytogenes (scientific name: Listeria monocytogenes) is a facultative anaerobic bacterium that is t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/327C23C14/34C23C14/18C23C14/02
CPCG01N27/26G01N27/327
Inventor 李承勇周浓周春霞王娟
Owner GUANGDONG OCEAN UNIVERSITY
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