Glutathione reductase detection kit and application
A detection kit, glutathione technology, applied in the field of biochemical detection, can solve the problems of low cost, cumbersome steps, high cost, etc., and achieve the effect of facilitating two-point calibration, improving linear relationship, and reducing influence
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Embodiment 1
[0042] A glutathione reductase detection kit, comprising the following components:
[0043] First reagent, including phosphate buffer, EDTA-NA2, ascorbate oxidase, potassium ferricyanide, preservatives and oxidized glutathione;
[0044] Secondary reagents, including tris buffer, EGTA, preservatives and reduced coenzyme.
[0045] The advantages of the present invention are:
[0046] (1) Both the first reagent and the second reagent are in liquid state, and can be stored at 2-8°C for 12 months after thermal stability test with thermal acceleration;
[0047] (2) Potassium ferricyanide in the first reagent can oxidize ferrous ions (Fe2+) in hemoglobin to ferric ions (Fe3+), and hemoglobin is converted into methemoglobin. Methemoglobin combines with cyanide ion (CN-) to generate stable cyanide hemoglobin, which has no effect on the detection of glutathione reductase;
[0048] (3) The use of EGTA as the second reagent can improve the linear relationship between the detection resu...
Embodiment 2
[0069] This embodiment is basically the same as Embodiment 1, the difference is:
[0070] In the second reagent, the concentration of EGTA is 4 mmol / L.
Embodiment 3
[0072] This embodiment is basically the same as Embodiment 1, the difference is:
[0073] In the second reagent, the concentration of EGTA is 6 mmol / L.
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