85 results about "Glutathione reductase" patented technology

A compound of formula (I): wherein one of m and n represents 0, and the other represents 0, 1 or 2; k represents 0 or 1 to 12; R1 is hydrogen, an aryl, a heterocyclic, an alkyl, a hydroxy or -OR7, wherein R7 is an alkyl, an alkenyl or an aralkyl; A is -CON(R2)SO2-, wherein R2 is hydrogen, an alkyl or an aralkyl; B is a single bond; and pharmaceutically acceptable salts thereof. The compounds have the ability to enhance the activity of glutathione reductase and can therefore be used for the treatment and prevention of a variety of diseases including cataracts.

An intracellular selection system allows screening for peptide bioactivity and stability. Randomized recombinant peptides are screened for bioactivity in a tightly regulated expression system, preferably derived from the wild-type lac operon. Bioactive peptides thus identified are inherently protease- and peptidase-resistant. Also provided are bioactive peptides stabilized by a stabilizing group at the N-terminus, the C-terminus, or both. The stabilizing group can be a small stable protein, such as the Rop protein, glutathione sulfotransferase, thioredoxin, maltose binding protein, or glutathione reductase, an α-helical moiety, or one or more proline residues.

The present invention relates to a kit for the treatment of cancer comprising (a) a container for containing a first compound (i) or a precursor thereof, said first compound or precursor being a compound that oxidizes glutathione (GSH); (b) a container for containing a second compound (ii) or a precursor thereof, said second compound or precursor being a compound that forms an adduct or conjugate with GSH; (c) a container for containing a third compound (iii) or a precursor thereof, said third compound or precursor being a compound that inhibits the rate-limiting enzyme of GSH biosynthesis, gamma-glutamylcysteine synthetase (GCS); and (d) a container for containing a fourth compound (iv) or a precursor thereof, said fourth compound or precursor being a compound that inhibits the enzyme responsible for the conversion of GSSG to GSH, glutathione reductase (GR).

This invention relates to methods of expressing eukaryotic proteins in prokaryotic hosts, particularly eukaryotic proteins that require formation of disulfide bridges for biological activity. Various approaches are used including fusion to thioredoxin, cytoplasmic expression of disulfide isomerases, deficiencies in thioredoxin and / or glutathione reductases, deficiencies in proteases, and the like. The method is applicable to express monomeric and dimeric forms of the eukaryotic protein with biological activity such as monomeric and dimeric forms of a disintegrin or a disintegrin domain. Included are the vectors, host cells expressing the proteins, the expressed proteins and methods of using the proteins.

An intracellular selection system allows screening for peptide bioactivity and stability. Randomized recombinant peptides are screened for bioactivity in a tightly regulated expression system, preferably derived from the wild-type lac operon. Bioactive peptides thus identified are inherently protease- and peptidase-resistant. Also provided are bioactive peptides stabilized by a stabilizing group at the N-terminus, the C-terminus, or both. The stabilizing group can be a small stable protein, such as the Rop protein, glutathione sulfotransferase, thioredoxin, maltose binding protein, or glutathione reductase, an α-helical moiety, or one or more proline residues.

The invention relates to a fluorescent probe for detecting glutathione reductase and a bioactive sulfhydryl compound as well as a synthesis method and application thereof. In the invention, a coumarin-3-carbonyl compound with substitutional groups R1, R2, R3 and R4 is dissolved in a dry organic solvent, and o-aminothiophenol with a substitutional group R5 is slowly dropped into the mixture at room temperature or circumfluence temperature; the mixture is stirred and filtered, the organic solvent is removed, and then a probe precursor is obtained after a solid is obtained by vacuum drying; the probe precursor is dissolved in the dry organic solvent, and HgX2 is slowly dropped into the mixture under an ice bath condition; and the mixture is stirred to obtain a red solid deposit, a red solid is obtained by vacuum drying after the red solid deposit is filtered, then diffusion crystallization is carried out by using the organic solvent, and a red acicular crystal of formula (I) is obtained, i.e. the fluorescent probe. The fluorescent probe has favourable selectivity to the glutathione reductase and the bioactive sulfhydryl compound, and is suitable for the fast detection of the glutathione reductase and the bioactive sulfhydryl compound.

The invention relates to an isaria cosmopaltriae yasuda polysaccharide and applications thereof in preparing a nerve-protective and anti-aging drug. The isaria cosmopaltriae Yasuda polysaccharide is obtained by adopting the following method: drying isaria cosmopaltriae yasuda materials, grinding and sieving with a pharmacopoeia sieve NO.2, and refluxing and extracting powder twice with water at 90 DEG C, wherein each time lasts for two hours; performing decompression concentration to an extracting liquid, then adding anhydrous ethanol, and settling overnight at 4DEG C-25DEG C; centrifuging for 10-30min at 3000r / min; and drying the settling part to obtain isaria cosmopaltriae yasuda polysaccharide (JCHCPS). The isaria cosmopaltriae yasuda polysaccharide can obviously inhibit aging injury of glutamic acid-induced PC12 cells, stop the release of cells LDH, improve the survival rate of the cells, reduce the level of oxygen radicals in the cells, and improve the activity of glutathione reductase (GSH-Px) and superoxide dismutase (SOD), and shows better antioxidant activity in experiments of clearing DPPH.superoxide anion (O2.-).

The invention discloses a freckle expelling facial mask which is prepared by soaking facial mask paper into liquid prepared from the following raw materials in parts by weight: 0.1-3 parts of micromolecular hyaluronic acid, 0.1-2.5 parts of macromolecular hyaluronic acid, 0.7-30 parts of synthesized egg white powder, 1-7 parts of an aloe extract, 0.7-9 parts of propylene glycol, 5-50 parts of 1,2-butanediol, 5-30 parts of glycerol, 20-80 parts of a ginseng extract, 20-50 parts of a bletilla striata extract, 10-50 parts of a leonurus extract, 30-50 parts of a white mulberry extract, 50-100 parts of a coix seed extract, and the balance of water which is added till being 1000 parts, wherein the aloe extract is powder or granules, and the water can be deionized water, distilled water or purified water. The freckle expelling facial mask disclosed by the invention has the effects of removing superoxide free radicals, activating glutathione reductase, suppressing activity of tyrosinase in skin melanocyte, remedying damaged skin and the like, has the functions of expelling freckles and preventing black spot regeneration, is free of irritation on skin, and is applicable to all skin types.

Disclosed are stable recombinant multi-gene nucleic acid constructs, such as plant binary vectors, comprising (i) a gene encoding gamma-glutamylcysteine synthetase and (ii) a gene encoding glutathione synthetase, plus preferably at least one, preferably two, genes which encode enzymes involved in the redox cycling of glutathione between its reduced and its oxidised forms e.g. glutathione reductase and / or glutathione peroxidase. Preferably the promoters linked to the genes are different and of different strengths, and may optionally be inducible. Also provided are related materials and corresponding methods and uses e.g. in plants to improve oxidative stress tolerance enhance root development, or to increase the post-harvest shelf life of the plant or part thereof.

The mechanism of action of Ebselen differentiates between bacterial and mammalian thioredoxin reductase (TrxR). It displays fast oxidation of mammalian Trx and via the NADPH-TrxR catalyzed turnover of ebselen selenol with hydrogen peroxide, and therefore are mammalian antioxidants. Ebselen, and its diselenide, are strong competitive inhibitors of E. coli TrxR with Ki of 0.14 μM and 0.46 μM, respectively. E. coli mutants lacking glutathione reductase or glutathione were much more sensitive to inhibition by ebselen. Since either glutaredoxin or thioredoxin systems are electron donors to ribonucleotide reductase, ebselen targets primarily glutathione and glutaredoxin-negative bacteria, a class which includes major pathogens. Ebselen, and similar compounds are therefore useful as antibacterial agents, even for multiresistant strains. Two major pathogenic bacteria, which previously had not been known to be sensitive to ebselen, Mycobacterium tuberculosis (tuberculosis) and Helicobacter pylori (stomach ulcer and cancer), were shown to be excellent targets. Helicobacter pylori was also sensitive to ebsulfur.

The invention provides a low-temperature preservation culture medium of rubber tree embryonic callus and a low-temperature method of the culture medium, belonging to the technical field of tissue culture. Exogenous antioxidant L-glutamine, oxidation-resistant vitamin VC and exogenous hormone salicylic acid are added in the low-temperature preservation culture medium; endogenous antioxidant enzyme glutathione reductase GR action is motivated to maintain intracellular high-proportion GSH / GSSG and SG so as to participate in ascorbic acid glutathione circulation AsA-GSH of a key approach for eliminating ROS in a plant, other antioxidant enzyme is supplemented to synergistically and effectively remove residual ROS in the embryonic callus preserved at low temperature so as to improve the cold stress resistance of the embryonic callus. The callus survival rate of the embryonic callus preserved by a low-temperature preservation culture medium is not significantly different from that of the embryonic callus cultured in a dark condition at 25 plus / minus 2 DEG C, and the FDA fluorescence detection indicates that the cell viability of the embryonic callus subjected to low-temperature preservation is maintained stronger.

Compositions and methods are provided for the enhanced in vitro synthesis of active polypeptides containing disulfide bonds. In certain embodiments of the invention, the reaction mix includes a biological extract derived from a bacterial cell in which the glutathione reductase gene has been inactivated, which is pre-treated with a low concentration of a sulfhydryl inactivating agent.

Compositions and methods are provided for the enhanced in vitro synthesis of active polypeptides containing disulfide bonds. In certain embodiments of the invention, the reaction mix includes a biological extract derived from a bacterial cell in which the glutathione reductase gene has been inactivated, which is pre-treated with a low concentration of a sulfhydryl inactivating agent.

Food spoilage leads to food wastage, human morbidity and mortality. This food preservation takes advantage of use of Hydrogen Sulfide without or with Hydrogen or Helium into an environment where food is stored. The method delays food ripening, food spoilage, food decay and is safe, and preserves the natural characteristics of food, including color, flavor, aroma and texture. Hydrogen Sulfide treatment maintains higher activities of catalase, guaiacol peroxidase, ascorbate peroxidase, glutathione reductase and lower activities of lipoxygenase relative to un-treated controls. Hydrogen Sulfide also reduces malondialdehyde, Hydrogen peroxide, and superoxide anion to levels below those in control fruits during storage. Hydrogen and Helium are administered as gas and Hydrogen Sulfide is administered as a gas, liquid, or a Hydrogen Sulfide donor, within a closed environment or by providing Hydrogen Sulfide within the item. This also includes enhancement of innate endogenous Hydrogen Sulfide-Hydrogen production in the organisms including plants.

The invention relates to a Cordyceps sobolifera active site and application thereof in preparing drugs for nerve protection and aging resistance. The active site is an n-butanol site of a Cordyceps sobolifera water extract. The active site is prepared by the following steps: pulverizing Cordyceps sobolifera, extracting with water under reflux, concentrating the extracting solution under reduced pressure, adding anhydrous ethanol, and precipitating over night; centrifuging, and concentrating; and carrying out fractional extraction on the concentrated solution with petroleum ether, ethyl acetate and n-butanol, concentrating under reduced pressure to recover the solvent, and carrying out freeze-drying to respectively obtain a petroleum ether site, an ethyl acetate site, an n-butanol site and a water site. The n-butanol site can obviously inhibit aging and damage of the glutamic-acid-induced PC12 cells, prevent the cell LDH from release, enhance the survival rate of the cells, lower the intracellular oxygen free radical level, enhance the activity of the glutathione reductase (GSH-Px) and superoxide dismutase (SOD), and have favorable in-vitro oxidation resistance activity in the experiment of clearing DPPH. and superoxide anion (O<2.->) free radicals.

The invention relates to the technical field of immobilized enzymes, in particular to a metal organic framework material-enzyme compound as well as a preparation method and application thereof. The preparation method of the metal organic framework material-enzyme compound comprises the following steps that antioxidant enzyme, transition metal ions and an organic ligand are dissolved in a solvent for a co-precipitation reaction, the antioxidant enzyme is selected from combined enzyme of superoxide dismutase and catalase, glutathione peroxidase or horseradish peroxidase, and the transition metal ions are selected from soluble metal salts, and the organic ligand is an imidazole compound. The metal organic framework material-enzyme compound has strong active oxygen scavenging capacity.

The invention discloses a method for screening glutathione reductase inhibitors based on fluorescence recovery of carbon dots. Yellow fluorescence carbon dots for Ag<+> ion specific quenching serve asa fluorescence probe; glutathione reductase can perform catalytic reduction on oxidized glutathione to produce reduced glutathione in the presence of a coenzyme NADPH (Nicotinamide Adenine Dinucleotide Phosphate), and the reduced glutathione can perform competitive binding with the Ag<+> ion so as to further recover fluorescence of the carbon dots; and the glutathione reductase treated and inactivated by the glutathione reductase inhibitor cannot perform catalytic reduction on the oxidized glutathione, therefore, the fluorescence of the carbon dots cannot be recovered. On this basis, screening of the glutathione reductase inhibitors can be realized by virtue of the fluorescence recovery situation. The method for screening glutathione reductase inhibitors disclosed by the invention has thecharacteristics of being high in sensitivity, excellent in selectivity, low in cost, low in toxicity, environment-friendly, high in stability and the like, and has significances for screening relateddrugs based on the glutathione reductase inhibitor.

The present invention is psychrophilic glutathione reductase and its preparation process. The psychrophilic glutathione reductase is separated and purified in a four-step process including ammonium sulfate precipitation, ion exchanging, affinity chromatography and gel filtering. It consists of two identical subunits of apparent molecular weight 54.6 kDa each, and has optimal temperature of 25 deg.c, optimal pH value of 7.5, optimal Mg2+ concentration of 7.5 mmol / L, and promotion effect of Ca2+ and Mg2+. The psychrophilic glutathione reductase may be well used in low temperature enzyme catalysis mechanism research, in resistant variety improvement, etc.

The invention discloses a size-controllable synthesis method for MSe (M equal to Cd, Pb) nanocrystals. By regulating the pH value of a system, the method simulates the optimal conditions of the reduction process of catalyzing sodium selenite (Na2SeO3) in vivo with glutathione reductase and coenzyme II outside cells to obtain low-valent Se, the low-valent Se reacts with glutathione-coordinated M2 plus ([M-(GS)2]2 plus) under the inert atmosphere, and thereby MSe (M equal to Cd, Pb) nanocrystals with good monodispersity, uniform grain size and fluorescence are obtained under the aqueous-phase condition. The method can control the size of the product by regulating the proportion between the Se precursor and the M precursor. The method is simple, and can repetitively prepare a large quantity of MSe (M equal to Cd, Pb) nanocrystals, moreover, because flammable, explosive and toxic metal organic compound is not used, safety is high, and the method can be widely applied to the field of chemical and material science.

The present invention concerns the use of glutathione-reductase (GSSG reductase) for the preparation of a medicament for the treatment of HIV-infected patients, seropositive or already affected by AIDS, both for prophylactic and therapeutic use.

The invention discloses a glutathione reductase testing reagent quality control product which is characterized by comprising freeze dried products of the following components: 50-80 parts of a phosphate buffer, 5-10 parts of glycine, 1-3 parts of xanthan gum, 5-10 parts of bovine serum, 0.1-0.5 part of a pure product of glutathione reductase, 2-5 parts of cane sugar and 1-2 parts of a preservative. The quality control product is a freeze dried product, is capable of greatly improving the stability of the quality control product before use and is easy to preserve. The invention further discloses a preparation method of the quality control product. The preparation method comprises the following steps: adding the pure product of glutathione reductase into the phosphate buffer so as to obtaina diluted liquid; adding the glycine into the diluted liquid, performing heat-preservation stirring, and equally dividing the obtained mixed solution into a first mixed liquid and a second mixed liquid; adding the bovine serum and the cane sugar into the first mixed liquid so as to obtain a first freeze dried liquid; adding the bovine serum, the xanthan gum and the cane sugar into the second mixedliquid so as to obtain a second freeze dried liquid; and mixing the first freeze dried liquid with the second freeze dried liquid, and performing freeze drying, so as to obtain the quality control product.

The invention discloses a kit for determining glutathione reductase. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises a buffer solution, oxidized glutathione, a surfactant and EGTA, and the reagent R2 comprises a buffer solution, a reducing coenzyme II tetrasodium salt and a surfactant. By adding quantitative polyoxyethylene ether Brij58 into the reagent R1 and the reagent R2, interference of hemoglobin can be greatly reduced, the anti-interference capability reaches 5.0 g / L, and the detection accuracy of a hemoglobin abnormal sample is greatly improved; meanwhile, quantitative EGTA is added into the reagent R1, so that the problem that the enzyme activity is reduced due to the fact that glutathione reductase in a fresh sample mainly exists in a polymerase form is solved, and the clinical detection accuracy of the kit is effectively improved.

The invention discloses instant clear soup crystal noodles, which comprise the following raw materials in parts by weight: 20-40 parts of sweet potato powder, 2-3 parts of wheat germ oil, 2-3 parts of chicken oil, 20-30 parts of purple sweet potato starch, 15-22 parts of mashed potato, 1-2 parts of soybean protein, 5-8 parts of dextrin powder, 8-14 parts of kudzuvine root powder, 0.2-0.3 part of isomaltose oligosaccharide, 0.02-0.05 part of glutathione reductase, 1-2 parts of cooked duck egg yolk powder, 1-2 parts of corn powder, 1-2 parts of pork liver, 2-5 parts of lychee exocarp powder, 2-5 parts of chestnut shell powder, 0.5-1 part of herba ecliptae, 0.5-1 part of plumula nelumbinis, and 0.5-1 part of xylitol. After opening the bag, a proper amount of cold water is added, the crystal noodles can be softened for eating, the operation is very convenient, and the crystal noodles are good in quality, easy to digest, and cannot harm the esophagus mucous membrane; the polymerizability of the crystal noodles is good, cannot loosen after being immersed into water for a long time, and cannot go bad when the crystal noodles are immersed into the water and stored for three days; and the seasoner is light and delicious without the flavor of traditional instant noodle seasoner, and cannot make users feel sick after eating, and the phenomena of stomachache and dry stool cannot be caused.

The invention provides a glutathione reductase assay kit, which comprises a reagent R1 and a reagent R2. The reagent R1 includes: 50-200 mmol / L of a Tris buffer solution, 0.5-2 mmol / L of EDTA, 1-5 kU / L of pyruvic carboxylase, 1-5 kU / L of ascorbic acid oxidase, 5-20 mg / L of potassium ferrocyanide, a surfactant and a preservative; the reagent R2 includes: 50-200 mmol / L of the Tris buffer solution, 0.5-2 mmol / L of EDTA, 2-10 mmol / L of GSSG, 0.1-0.5 mmol / L of NADPH, 1-15 g / L of a stabilizer, and 0.5-2 g / L of a preservative. The liquid assay kit has good stability and is strong in anti-interferenceeffect. The invention also discloses a preparation method and an application of the assay kit.

The invention provides a glutathione reductase detection kit and a preparation method and application thereof. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises a potassium phosphate buffer solution, EDTA, GSSG, ascorbic acid oxidase, bilirubin oxidase, potassium ferricyanide, a surfactant, a first bacteriostatic agent and a second bacteriostatic agent; and the reagent R2 comprises a sodium bicarbonate buffer solution, NADPH, the first bacteriostatic agent, a surfactant and a stabilizer. Compared with the prior art, the glutathione reductase detection kit provided by the invention has the advantages of good airborne stability, high sensitivity and strong antibacterial ability, and can meet requirements of clinical application of glutathione reductase detection.

The invention discloses a diluent formula for improving the frozen quality of yak frozen semen. Each 100 milliliters of frozen semen diluent is prepared from 0.9 to 1.2 grams of sodium citrate, 3 to 5grams of saccharose, 6 to 8 milligrams of low density lipoprotein, 4 to 7 milliliters of glycerinum, 6 to 8 milligrams of resveratrol, 5 to 8 milligrams of melatonin, 35 milligrams of spectinomycin and 80 thousand units of gentamicin. According to the diluent formula disclosed by the invention, the low density lipoprotein, the resveratrol and the melatonin are jointly added into a semen diluent for the first time, and a diluent preparation method is corrected. Experimental data show that the frozen semen diluent not only can improve sperm motility and acrosomal intactness rate of frozen-thawed semen, but also improves activity of catalase (CAT), glutathione peroxidase (GR) and glutathione reductase (GSH) in seminal plasma which are used for protecting sperm fertilizing ability; furthermore, sperm DNA integrity is improved, and the fact that the semen formula can improve the frozen quality of semen is ensured.