Diluent formula for improving frozen quality of yak frozen semen

A technology of freezing semen and diluent, which is applied in the field of agricultural livestock, can solve the problems affecting artificial insemination, low vitality, low fertilization rate, etc., and achieve the effect of improving integrity, improving quality, improving sperm motility and acrosome integrity

Inactive Publication Date: 2019-01-25
QINGHAI UNIVERSITY +1
View PDF13 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in production practice, there are often cases of low vitality and low fertilizatio

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Diluent formula for improving frozen quality of yak frozen semen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0015] The present invention will be further described below:

[0016] According to the technical scheme of the present invention, the substances needed to prepare 100 ml of frozen semen dilution are: sodium citrate: 0.9-1.2 grams, sucrose: 3-5 grams, low-density lipoprotein: 7-9 mg, glycerol: 4-5 grams 7 ml, resveratrol: 6-8 mg, melatonin: 5-7 mg, spectinomycin: 35 mg, gentamicin: 80,000 units (IU).

[0017] Its best formula for frozen semen dilution is: the content of substances per 100 ml is: sodium citrate: 1.073 mg, sucrose: 4.344 mg, low-density lipoprotein: 8 mg, glycerin: 6 ml, resveratrol : 7 mg, melatonin: 5 mg, spectinomycin: 35 mg, gentamicin 80,000 units (IU).

[0018] Its preparation method is:

[0019] 1) Weigh the raw materials in the formula: sodium citrate, sucrose, low-density lipoprotein, glycerin, resveratrol, melatonin, distilled water.

[0020] 2) Prepare the above sodium citrate, sucrose, low-density lipoprotein, melatonin, resveratrol, glycerin, and...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a diluent formula for improving the frozen quality of yak frozen semen. Each 100 milliliters of frozen semen diluent is prepared from 0.9 to 1.2 grams of sodium citrate, 3 to 5grams of saccharose, 6 to 8 milligrams of low density lipoprotein, 4 to 7 milliliters of glycerinum, 6 to 8 milligrams of resveratrol, 5 to 8 milligrams of melatonin, 35 milligrams of spectinomycin and 80 thousand units of gentamicin. According to the diluent formula disclosed by the invention, the low density lipoprotein, the resveratrol and the melatonin are jointly added into a semen diluent for the first time, and a diluent preparation method is corrected. Experimental data show that the frozen semen diluent not only can improve sperm motility and acrosomal intactness rate of frozen-thawed semen, but also improves activity of catalase (CAT), glutathione peroxidase (GR) and glutathione reductase (GSH) in seminal plasma which are used for protecting sperm fertilizing ability; furthermore, sperm DNA integrity is improved, and the fact that the semen formula can improve the frozen quality of semen is ensured.

Description

technical field [0001] The invention relates to the technical field of agricultural livestock, in particular to a diluent formula for improving the frozen quality of yak frozen semen. Background technique [0002] The successful freezing of domestic animal semen has had a profound impact on the development of artificial insemination technology. Firstly, it has laid the foundation for expanding the utilization rate and prolonging the utilization time of excellent breeding males, and secondly, it has provided a broad space for the development of artificial insemination and cryobiology. Because frozen semen is more convenient in transportation, no matter in terms of time or space, the mating efficiency of male animals can be fully exerted, and the pace of livestock breed improvement has been accelerated. Although all countries have launched research on frozen semen of cattle, pigs, horses, and chickens, only the artificial insemination technology of frozen bovine semen is widel...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 桂林生韩银仓孙永刚赵宪林
Owner QINGHAI UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap