25 results about "Bovine semen" patented technology

The invention discloses a long-chain non-coding RNA molecular marker related to bovine semen quality and application of the long-chain non-coding RNA molecular marker, long-chain non-coding RNA comprises an LNC68393 promoter sequence and an LNC68393 gene sequence, a molecular marker SNP1 is located on the promoter sequence, and a molecular marker SNP2-5 is located on the gene sequence. Different polymorphisms of the molecular marker are significantly related to the bull semen quality, and the molecular marker can be applied to detection of the bull semen quality, further early evaluation of the bull reproductive performance, cultivation of excellent bulls, shortening of the breeding time, improvement of the efficiency and reduction of the cost, and has important application value in improvement of the reproductive performance of the whole bull herd.

The invention discloses a bull semen quality related SNP molecular marker based on a CCDC89 gene and application of the bull semen quality related SNP molecular marker, and belongs to the technical field of genetic molecular biology, the SNP molecular marker is located at the position of chr29: 9868636 of the bull CCDC89 gene, the gene polymorphism is G or A, and when the SNP site is G, high semen quality is shown. By detecting the polymorphism of the SNP site, the detection of the bull semen quality and the identification of the reproductive performance can be realized, the breeding is assisted, the reliable support is provided for breeding the next generation of high quality, and the SNP site has the advantages of high reliability, high efficiency, low cost and the like.

A method and device for predicting the bull semen quality. The method comprises: on the basis of the variation of methylation level values, using known methylation spectrum data of methylation sites of a bull sperm to select 83 methylation sites; on the basis of an elastic net regression algorithm, using the selected methylation sites to construct an epigenetic clock; and using the epigenetic clock to predict the age of a bull to be detected. The age of a bull can be predicted on the basis of an epigenetic clock, and the bull semen quality can be predicted on the basis of an epigenetic acceleration. The present invention can be applied to bull variety breeding and bull breeding.

The present application belongs to the technical field of bull semen quality evaluation, and particularly relates to a local bull semen quality evaluation method and system based on multi-source data analysis. The method comprises: when real-time data corresponding to a current semen sample is acquired, executing a quality evaluation process to generate a comprehensive score of the current semen sample; based on the offset amplitude of sample data generated by the evaluation model in the benchmark test, determining a main parameter and at least one subsidiary parameter from a plurality of to-be-tested parameters associated with the current semen sample. The present application calculates a subsidiary score according to the subsidiary parameter, and adjusts the subsidiary score by using an external parameter to generate a comprehensive score; and a multi-dimensional dynamic evaluation framework is constructed to consider a plurality of to-be-tested parameters of the semen itself, and the external parameter is quantified and integrated into the final score, so that the evaluation result can dynamically adapt to different environments and individual conditions, and overcome the limitation of single evaluation dimension in the prior art.

Disclosed are a method for predicting semen quality of a bull, a device therefor, and a computer-readable storage medium. The method screens 83 CpG sites from known methylation profile data of bull sperm based on variability in methylation levels, and constructs an epigenetic clock using elastic net regression with the screened CpG sites. The epigenetic clock is used to predict an age of a subject bull. Semen quality is predicted based on epigenetic age acceleration, defined as a difference between the predicted epigenetic age and a chronological age of the bull, and may be used for selection and breeding of bulls.

The invention provides a bovine semen freezing preservation method, and relates to the technical field of semen freezing. The bovine semen freezing preservation method mainly comprises the following steps: mixing folic acid, lycopene, anthocyanin and anethene for feeding in the earlier stage of cattle, manually collecting semen, adding caffeine, dendrophenol, chlorogenic acid and pinoresinol diglucoside into a basic semen diluent, diluting the bovine semen, pre-cooling the diluted bovine semen, and freezing the pre-cooled bovine semen. And finally, carrying out low-temperature treatment on the precooled seminal fluid and then carrying out liquid nitrogen freezing. The defects in the prior art are overcome, the influence of freezing on the activity of the bovine semen can be effectively reduced, the motility activity and plasma membrane integrity of the frozen bovine semen are guaranteed, and a good foundation is provided for bovine breeding work.

The invention discloses a yak frozen semen freezing process intelligent control method based on dynamic cooling curve optimization, and relates to the field of animal frozen semen preservation, and the yak frozen semen freezing process intelligent control method comprises the following steps: arranging a plurality of frozen semen tubes filled with consumed cattle semen on an annular tray with a cold source in the center in a radioactive manner, and uniformly arranging the plurality of frozen semen tubes in a surrounding manner in each radial distance interval; in the freezing process, cold energy is output according to a preset cooling program, and the annular tray is driven to rotate; unfreezing operation is executed, sperm motility values are measured, interval motility values of the sperm motility values of the frozen sperm tubules in all the radial distance intervals are counted, a temperature curve at the radial distance interval with the highest interval motility value is determined, a cooling program is modified in multiple rounds of experiments, and the process is repeated, so that the temperature curve is determined. And clustering the obtained temperature curves and selecting an optimal target temperature curve to perform freezing control. According to the method, the problem that the optimal dynamic cooling curve is difficult to efficiently obtain in the freezing process of the frozen yak sperms is solved.

The present invention provides an antifreeze polypeptide derived from bovine collagen, which can reduce the difference in solute concentration inside and outside the cell during the cooling process, thereby inhibiting the formation of ice crystals inside and outside the sperm cell, and further reducing ice crystal damage to cell membranes and organelles during deep cryopreservation and recovery, thereby improving the survival rate of sperm cells after deep cryopreservation and recovery. The frozen sperm has a higher in vitro fertilization cell development rate and fertility rate, and can reproduce rapidly. In addition, the semen freezing diluent provided by the present application does not contain any exogenous protein, which can effectively reduce exogenous protein interference and immunosuppression during in vitro fertilization, and further improve the fertilization rate and fertility rate of frozen semen.

The present invention provides a diluent for improving the quality and fertilization ability of frozen bovine semen, including chlorogenic acid and a calcium chelator. Compared with the prior art, the present invention adds antioxidant chlorogenic acid or caffeic acid and a calcium chelator to the semen cryopreservation diluent, effectively improves the vitality, plasma membrane integrity, acrosome integrity and fertilization ability of frozen-thawed semen, significantly improves the antioxidant performance, reduces the ROS level, inhibits premature capacitation at low temperature, and significantly improves the cryopreservation effect of bovine sperm. It is suitable for long-term cryopreservation of bovine semen in liquid nitrogen and artificial insemination. The diluent preparation method is simple, the cryopreservation operation method is simple, and it is easy to promote, and significantly improves the quality and fertilization ability of bovine semen after freezing and thawing.

The invention relates to the technical field of semen freezing equipment, and particularly discloses a self-cleaning type bovine semen cryopreservation device which comprises a freezing box and a cryopreservation unit arranged in the freezing box, and the cryopreservation unit comprises a semen storage pipe, a horizontally-arranged circular supporting seat, a plurality of brush assemblies, a driving mechanism and an anti-rotation mechanism. The brush assemblies are evenly distributed in the circumferential direction of the circular supporting seat and assembled on the circular supporting seat in a sliding mode in the radial direction of the circular supporting seat. The driving mechanism comprises a first driving assembly used for driving the circular supporting base to ascend and descend and rotate around the axis of the circular supporting base. The driving mechanism further comprises a second driving assembly which drives the brush assemblies to get close to each other when the circular supporting base descends and to get away from each other when the circular supporting base ascends. According to the self-cleaning type bovine semen cryopreservation device, pollutants such as dust on the inner wall of the semen storage pipe and frost on the outer wall of the semen storage pipe can be swept away, the self-cleaning function is achieved, semen can be prevented from being polluted, and the anti-skid function is achieved.

The utility model relates to the technical field of bovine semen freezing tubes, and discloses a bovine semen freezing tube spiral anti-shaking fixing frame which comprises a supporting plate, long plates are fixedly connected to the left side and the right side of the top of the supporting plate, T-shaped plates are fixedly connected to the tops of the long plates, long plates are fixedly connected to the tops of the T-shaped plates, and the long plates are fixedly connected to the tops of the T-shaped plates. The middle of the inner side of each long plate is in threaded connection with a threaded rod, a connecting column is fixedly connected between every two adjacent long plates, the other end of each connecting column is fixedly connected with an arc-shaped plate, and the front end and the rear end of the adjacent side of each long plate are fixedly connected with limiting rods. According to the long freezing pipe fixing device, the long plate is matched with the T-shaped plate to be fixed, the top of the T-shaped plate is connected with the long plate, the two arc-shaped plates are matched to run oppositely, and then the long freezing pipe is fixed, so that the stability of the long freezing pipe in the using process can be effectively ensured, and the shaking condition is avoided; and certain stability is improved.

This utility model relates to a bovine semen storage box capable of precise temperature control, belonging to the technical field of frozen semen storage devices. This utility model effectively reduces the intrusion of external heat through a double-layer heat insulation structure of the outer casing combined with a top heat insulation plate. Simultaneously, the distributed layout of semiconductor cooling chips, combined with real-time temperature monitoring by temperature sensors, ensures uniform and stable temperature throughout the box. The rotating design of the cylindrical tray rack, combined with a pop-out mechanism, enables convenient loading and unloading of sample tubes, avoiding frequent opening of the box lid. This reduces interference with the internal temperature during sample storage and retrieval, significantly improving the reliability of bovine semen storage and sperm viability.

The present invention relates to the field of embryo engineering technology, and specifically relates to an embryo culture method for improving the in vitro blastocyst rate of cattle, which comprises the steps of: adding a set volume of maturation medium into each well of a four-well plate; placing the cumulus-oocyte complexes collected from cattle ovaries into the maturation medium for culture; adding the treated cattle semen into the fertilization medium containing the cumulus-oocyte complexes, and performing fertilization in an incubator; making a three-dimensional columnar culture droplet in the culture well to achieve the culture purpose of enabling the embryos to be in close contact, and increasing the paracrine between the embryos; and transferring the fertilized eggs into the three-dimensional columnar culture droplet for culture. The purpose of the embryo culture method for improving the in vitro blastocyst rate of cattle is to solve the problem of the relatively low blastocyst rate of cattle embryos cultured in vitro.

The invention relates to the technical field of embryo engineering, in particular to an embryo culture method for improving the bovine in-vitro blastocyst rate, which comprises the following steps: adding a maturation solution with a set volume into each hole in a four-hole plate; putting the collected cumulus-oocyte complex on the bovine ovary into a maturation solution, and culturing the cumulus-oocyte complex; adding the treated bovine semen into a fertilization liquid containing a cumulus-oocyte complex, and carrying out fertilization in an incubator; three-dimensional cylindrical culture liquid drops are manufactured in the culture holes, so that the culture purpose of tight contact between embryos is achieved, and paracrine between the embryos is increased; and transferring the fertilized ovum into a three-dimensional cylindrical culture liquid drop for culturing. The embryo culture method for improving the bovine in-vitro blastocyst rate aims at solving the problem that the bovine embryo in-vitro culture blastocyst rate is low.

The efficient and energy-saving bovine semen freeze-drying equipment comprises a box body and a box cover hinged to the top of the box body, a sealing plate is fixedly connected to the interior of the box body, a partition plate of a circular ring structure is fixedly connected to the bottom of the sealing plate, and a liquid nitrogen freezing chamber and a drying chamber are formed in the box body through the partition plate; a plurality of first placing assemblies for placing semen storage test tubes are uniformly arranged in the liquid nitrogen freezing chamber; the first placing assembly comprises a freezing pipe fixedly connected to the interior of the sealing plate, a fixed rod fixedly connected to the interior of the freezing pipe and arranged close to the bottom, a movable rod slidably connected to the interior of the fixed rod, and a bottom plate fixedly connected to the bottom end of the movable rod and attached to the bottom of the freezing pipe. By means of the structure, internal environment fluctuation caused by frequent taking and placing of the semen storage test tube is effectively prevented, and therefore the quality and stability of frozen semen are guaranteed; and meanwhile, the good sealing performance is favorable for reducing the consumption of liquid nitrogen, so that the efficient and energy-saving effects are achieved.

The present invention discloses long non-coding RNA molecular markers related to bovine semen quality and their applications. The long non-coding RNA includes the promoter sequence of LNC68393 and the gene sequence of LNC68393. The molecular marker SNP1 is located on the promoter sequence, and the molecular markers SNP2-5 are located on the gene sequence. Different polymorphisms of the molecular markers are significantly correlated with bull semen quality, and can be applied to detect bull semen quality, thereby early evaluating the reproductive performance of bulls, breeding excellent bulls, shortening the breeding time, improving efficiency and reducing costs, and having important application value for improving the reproductive performance of the entire cattle herd.

Provided are a cryopreservation liquid for bovine reproductive cells such as bovine sperms and a cryopreservation method thereof. Adopted is a cryopreservation liquid comprising: 0.3 to 0.9 w / w % of an amphoteric polyelectrolyte (an antifreeze polyamino acid), which is ε-poly-L-lysine having a number average molecular weight of 1,000 to 20,000 wherein 50 to 99 mol % of amino groups of the ε-poly-L-lysine are blocked as carboxylated by having been reacted with the succinic anhydride; and 2 to 4 w / w % of glycerol, as dissolved in a physiological solution. A preferred embodiment of the cryopreservation method comprises steps of: primary diluting, in which bovine semen is diluted to 2.5 to 10 times with a physiological solution and kept at 2 to 8° C.; and secondary diluting, in which the bovine semen is diluted to 5 to 20 times while being kept at 2 to 8° C., by adding dropwise a physiological solution containing the amphoteric polyelectrolyte (antifreeze polyamino acid) and glycerol to a suspension obtained in the primary diluting.

The invention provides a preparation device and method of frozen diluted semen of pigs, and relates to the technical field of animal husbandry engineering.The preparation device of the frozen diluted semen of the pigs comprises a rack; the shaking mechanism comprises a shaking table, a swinging frame, a rotating arm and a shaking motor, the swinging frame is hinged to the rack, the shaking table is hinged to the swinging frame, a connecting rod extending downwards is arranged at the bottom of the shaking table, the shaking motor is fixedly arranged on the rack, the rotating arm is connected with an output shaft of the shaking motor, a ball head sleeve is arranged on the rotating arm, and a ball head is arranged on the connecting rod; the ball head is spherically hinged with the ball head sleeve; the first heater is arranged on the shaking table; and the dilution cup is detachably connected with the first heater. The shaking mechanism drives the diluent and the fresh semen in the diluting cup to be mildly mixed and diluted, the whole liquid flows mildly, mixing is milder and more uniform, the structure and activity of the semen are protected physiologically, and meanwhile the device has the practical operation advantages of being convenient to operate and free of equipment pollution risk.

The invention belongs to the technical field of cryopreservation of sperms, and particularly discloses application of an L-proline oligomer 8 in preparation of a bovine semen freezing diluent. The invention provides application of the L-proline oligomer 8 in preparation of the bovine semen freezing diluent, and further provides the bovine semen freezing diluent which comprises the L-proline oligomer 8 and a basic diluent. The invention discloses an application of an L-proline oligomer 8 in preparation of a bovine semen freezing diluent, and the L-proline oligomer 8 is added into the freezing diluent, so that sperm motility, motility rate, plasma membrane integrity and acrosome integrity can be effectively improved, the late apoptosis proportion of sperms is inhibited, and bovine sperm quality is improved.

The invention relates to the technical field of animal breeding, and discloses a yak X / Y sperm separation and enrichment method based on a TLR7 / 8 agonist R848 and a kit. The method comprises the following steps: treating the yak sperm by using a sperm sorting solution containing a TLR7 / 8 dual agonist R848, incubating under specific conditions, and separating and collecting by using a stratification phenomenon caused by activity difference of X and Y sperms, the concentration of the R848 in the separation liquid is 0.3 [mu] M to 0.7 [mu] M; according to the specific reproductive physiological characteristics of the yak sperms, the problem that the separation efficiency is low or the sperms are seriously damaged due to inapplicable parameters in the prior art is solved, and the technical scheme special for yak sex control is easy and convenient to operate, low in cost, high in separation efficiency and small in influence on the sperm quality.

The present invention discloses an apparatus, method, computer-readable storage medium, and application for predicting bull age using a sperm epigenetic clock. The method uses known methylation profile data from bull sperm methylation sites to screen 83 selected methylation sites based on the variability of methylation levels. Using the elastic network regression algorithm, the selected methylation sites are used to construct an epigenetic clock. This epigenetic clock can be used to predict the age of the bull being tested. The epigenetic clock can be used to predict the age of the bull, and the epigenetic acceleration can be used to predict the quality of the bull's semen. This method can be applied to bull breed selection and breeding.

The utility model relates to the technical field of livestock breeding, and discloses an anti-unfreezing quick positioning device for frozen taking of bovine semen, which comprises a triangular table, and is characterized in that the inner wall of the triangular table is rotatably connected with a movable column, the outer wall of the movable column is fixedly connected with a telescopic mechanism, the outer wall of the movable column is rotatably connected with a taking mechanism, and the taking mechanism is fixedly connected with the telescopic mechanism. And the top of the taking mechanism is fixedly connected with a pressing mechanism, the pressing mechanism is used for butt joint, the taking mechanism comprises a movable plate, the bottom of the movable plate is fixed to the outer wall of a movable column, and the outer wall of the top of the movable plate is rotationally connected with a first rotating shaft. When the test tube storage box is used, the fixing plate at the bottom of the triangular table is firstly ensured to be properly mounted, the spring columns in the hollow columns above the fixing plate are firmly connected, the base is fixed at the top ends of the spring columns, the butt-joint bin is arranged above the base, and the semi-arc plate above the butt-joint bin can be connected and fixed with a stored test tube.

The invention relates to the technical field of sample preservation devices, in particular to a yak semen sample preservation device and a cryopreservation method.The yak semen sample preservation device comprises a sample collection bottle, the top of the sample collection bottle is sleeved with a bottle cap in a threaded connection mode, the bottom end of the sample collection bottle is sleeved with a connecting ring in a threaded connection mode, and a protective inner pipe and a protective outer pipe are fixed to the top face of the connecting ring; the top end of the protective outer pipe blocks the top end of the groove body between the protective inner pipe and the protective outer pipe; the device has the beneficial effects that a double-layer protection structure formed by the protection inner tube and the protection outer tube is arranged on the sample collection bottle in a sleeving manner through the connecting ring, and the connection stability and sealing performance are improved through the design of the rubber pad I and the rubber pad II, so that the sample collection bottle can be effectively prevented from being collided and damaged; the yak semen sampled in the sample collection bottle is effectively protected.

The utility model provides a bovine semen freezing box which comprises a supporting frame, two supporting legs which are symmetrically distributed left and right are arranged on the lower surface of the supporting frame, a box body is arranged in the supporting frame, a sliding opening is formed in the upper surface of the box body, and four sliding columns which are evenly distributed are arranged in the middle of the bottom end in the box body. A movable frame is slidably arranged among the outer arc surfaces of the four sliding columns, a sealing plate is arranged on the upper surface of the movable frame, a plurality of guide rails which are vertically and uniformly distributed are arranged on the left side and the right side in the movable frame, and a supporting plate, a freezing module and an electric control module are slidably arranged between every two left-right adjacent guide rails. By means of the bovine semen freezing box, cold air blown to the middle of the box body can be evenly blown to the bovine semen test tubes on the movable frame through the air guide plate which swings up and down in a reciprocating mode, and bovine semen in the bovine semen test tubes can be rapidly and efficiently cooled.

The present invention discloses a bull semen quality-related SNP molecular marker based on the CCDC89 gene and its application, belonging to the field of genetic molecular biology technology. The SNP molecular marker is located at the chr29:9868636 position of the bovine CCDC89 gene, and the gene polymorphism is G or A. When the SNP site is G, high semen quality is exhibited. By detecting the polymorphism of this SNP site, the bull semen quality can be tested and the reproductive performance can be identified, thereby assisting breeding and providing reliable support for breeding high-quality next generations. The method has the advantages of high reliability, high efficiency, and low cost.