30 results about "Bovine semen" patented technology

In the preferred embodiment, bovine semen is mixed with a suitable solution to form an insemination medium which is injected into the reproductive organs of a female bovine to flood the uterine horns in a nonsurgical AI procedure. The invention includes an AI instrument having a disposable, single use pipette that may be used with this nonsurgical procedure. The AI instrument does not include a balloon and is referred to as “catheter free”. Preferably, the uterine horns are flooded concurrently, but in the alternative, they may be flooded sequentially.

The invention provides a protective agent for prolonging the vitality retention period of fresh bovine semen, which is prepared by mixing a mother liquor 1, a mother liquor 2 and fresh egg yolk liquid according to a certain proportion. Due to the adoption of the protective agent provided by the invention, the vitality of fresh bovine semen can still be maintained to be higher than 65% within 16 hours after semen collection, and the semen mortality of the fresh bovine semen is controlled to be 5% to 13% within 16 hours after semen collection. The results show that by using the bovine semen protective agent provided by the invention, the vitality retention period of the fresh bovine semen can be effectively prolonged, so that the long-distance transport is convenient, and a technical support and guarantee is provided for subsequent semen freezing treatment or sex-controlling separation treatment.

The invention discloses a preparation method and a use method for EG (ethylene glycol) and TCM199 serving as bovine semen diluent and bovine semen freezing liquid. The preparation method for the EG and TCM199 serving as the bovine semen diluent and the bovine semen freezing liquid comprises the step of preparing the bovine semen diluent and the bovine semen freezing liquid. The use method for the EG and TCM199 serving as the bovine semen diluent and the bovine semen freezing liquid comprises the following steps of preparing the bovine semen diluent and the bovine semen freezing liquid; collecting semen; performing primary dilution; performing secondary dilution; performing freezing storage. According to the preparation method and the use method, the defreezing activity of frozen bovine semen is improved; the bovine artificial insemination conception rate is increased, the viscosity of the bovine semen diluent is lowered, and particles of the bovine semen diluent are reduced; a new-generation freezing agent with a small molecular weight and low toxicity is adopted to replace the original freezing agent with a large molecular weight and high toxicity; the operation is simplified; the preparation method and the use method are suitable for various types of large-scale production conditions.

The invention belongs to the technical field of cattle genetic marker preparation, and in particular relates to a genetic marker associated with semen quality traits. The marker is screened from the bovine FHL2 gene, and its sequence is 648bp-1170bp as shown in SEQ ID NO.1 In the nucleotide sequence of the sequence, a T / G base mutation occurs at the 879th base r of the sequence, resulting in a polymorphism of the FHL2 gene. The SNP typing detection technology of the promoter region of the bovine FHL2 gene is disclosed, and it is found that the T / G mutation site at the 879th base shown in SEQ ID NO.1 is significantly correlated with the average semen volume trait. The invention provides new genetic markers for marker-assisted selection of bovine reproductive traits.

The invention discloses an oil tea meal polypeptide, and a preparation method and an application thereof in cryopreservation of bovine semen. The oil tea meal hydrolytic polypeptide has a variety of activities, and the activities of the hydrolytic polypeptide are affected by proteolytic sites in the oil tea meal: the polypeptide produced by trypsin hydrolysis and having the molecular weight of [5000 u, 3000 u] has the effect of improving the antioxidant ability of the bovine cryopreserved semen, and can be used as a bovine semen cryopreservation protection agent for reducing oxidative damage;and the polypeptide produced by alkaline protease hydrolysis and having the molecular weight of [5000 u, 3000 u] can promote empathema and pregnancy of anestrus cows. Moreover, the preparation methodof the hydrolytic polypeptide is simple, only simple crushing, enzymatic hydrolysis, ultrafiltration and freeze drying are used, and all the steps can be realized in a factory with mass production andare easy to implement. Waste reuse of camellia meiocarpa hu. meal is realized, and the economic added value of the camellia meiocarpa hu. meal can be increased.

The invention discloses camellia meal polypeptides, a preparation method and application in promoting oestrus and conception of anestrus cows. The hydrolyzed polypeptides of camellia meal have variousactivities, and the activities of the hydrolyzed polypeptides are affected by proteolytic sites in the camellia meal: the polypeptide which is produced by hydrolysis of trypsin and has a molecular weight of (5,000 mu, 3,000 mu) has the effect of improving the oxidation resistance performance of bovine cryopreserved semen, and can be used as a bovine semen cryoprotective agent to reduce oxidativeinjury; the polypeptide which is produced by hydrolysis of alkaline protease and has a molecular weight of (5,000 mu, 3,000 mu) can promote the oestrus and the conception of the anestrus cows. Furthermore, the preparation method of the hydrolyzed polypeptides is simple, and only simple smashing, enzymolysis, ultrafiltration and lyophilization are used. The steps all can realize mass production ina factory and are easy to implement. According to the camellia meal polypeptides, the preparation method and the application in promoting the oestrus and the conception of the anestrus cows, waste reuse of small-fruit camellia meal is realized, and the economical additional value of the small-fruit camellia meal can be increased.

The invention discloses a bovine semen freezing dilute solution which contains a trace amount of rare-earth. The component of the bovine semen freezing dilute solution takes a commercially available cassou dilute solution as an application matrix and also contains the trace amount of rare-earth CeCl3 with the final concentration of 0.03-3 micromoles / liter. The invention discloses application of the bovine semen freezing dilute solution to cryopreservation of bovine semen. An experiment proves that the bovine semen freezing dilute solution disclosed by the invention accumulatively enhances five indexes for evaluating semen quality by 29% compared with a traditional freezing dilute solution; the bovine semen freezing dilute solution disclosed by the invention is easy to prepare, outstandingly superior to the traditional freezing dilute solution in total effect and worthy of popularization and application, and the source of the CeCl3 rare-earth guaranteed.

The invention discloses a preparation method and a using method for GL and TCM199 serving as bovine semen diluent and bovine semen refrigerating fluid. The preparation method comprises preparation of the bovine semen diluent and preparation of the bovine semen refrigerating fluid. The using method comprises the steps of preparing the bovine semen diluent and preparing the bovine semen refrigerating fluid; collecting semen; performing preliminary dilution; performing secondary dilution; and performing refrigeration storage. According to the preparation method and the using method, the defreezing activity of frozen bovine semen is improved, the bovine artificial insemination conception rate is increased, the viscosity of the bovine semen diluent is reduced, and the granular size of the bovine semen diluent is lowered; the operation is simplified; the preparation method and the using method can be used under various large-scale production conditions; the standardization and practicability level of refrigeration of the bovine semen in various scales and the defreezing activity of bovine capillary refrigerated semen are improved; the aberration rate of the defrozen semen is lowered, and the bovine artificial insemination conception rate is increased.

The invention provides a method for improving the sperm motility and increasing an in-vitro-fertilization rate of frozen bovine semen by the aid of genistein. The method is particularly characterized in that the genistein containing 1%o of DMSO (dimethyl sulfoxide) is added into SP-TALP (serum phosphate-total alkaline phosphatase) washing liquid for washing the frozen bovine semen in earlier-stage processing for the frozen bovine semen, or mixed liquid of PHE (phenylalanine) capacitation liquid and IVF-TALP (in vitro fertilization-total alkaline phosphatase) fertilization liquid containing bovine semen in a capacitation processing stage of second-stage pre-fertilization semen or prepared fertilization drops containing oocytes in a third-stage in-vitro-fertilization procedure; the added amounts of the genistein in two earlier stages of a fertilization procedure of each bottle of frozen bovine semen are 10 micromoles per liter, and the final concentration of the genistein in mixed liquid of the bovine semen and the fertilization drops containing the oocytes is 10 micromoles per liter when the genistein is added in a third stage of the fertilization procedure of each bottle of frozen bovine semen. The method has the advantages that the genistein is respectively used in the three in-vitro-fertilization stages, so that the sperm motility is greatly improved, and the sperm fertilization rate is greatly increased.

In the preferred embodiment, bovine semen is mixed with a “suitable solution” to form an insemination medium which is injected into the reproductive organs of a female bovine to flood the uterine horns in a nonsurgical AI procedure. The invention includes an AI instrument having a disposable, single use pipette that may be used with this nonsurgical procedure. The AI instrument does not include a balloon and is referred to as “catheter free”. Preferably, the uterine horns are flooded concurrently, but in the alternative, they may be flooded sequentially.