30 results about "Bovine semen" patented technology

In the preferred embodiment, bovine semen is mixed with a “suitable solution” to form an insemination medium which is injected into the reproductive organs of a female bovine to flood the uterine horns in a nonsurgical AI procedure. The invention includes an AI instrument having a disposable, single use pipette that may be used with this nonsurgical procedure. The AI instrument does not include a balloon and is referred to as “catheter free”. Preferably, the uterine horns are flooded concurrently, but in the alternative, they may be flooded sequentially.

In the preferred embodiment, bovine semen is mixed with a suitable solution to form an insemination medium which is injected into the reproductive organs of a female bovine to flood the uterine horns in a nonsurgical AI procedure. The invention includes an AI instrument having a disposable, single use pipette that may be used with this nonsurgical procedure. The AI instrument does not include a balloon and is referred to as “catheter free”. Preferably, the uterine horns are flooded concurrently, but in the alternative, they may be flooded sequentially.

The invention relates to the technical field of frozen cattle semen production and provides a diluent for frozen cattle semens. The diluent comprises a diluent A and a diluent B, wherein per 100ml of the diluent A comprises the following raw materials: 2.5-3.4g of trisodium citrate or sodium citrate, 0.1-0.2g of gentamycin, 0.03-0.1g of Ciprofloxacin, 50-100 million thousand units of ampicillin sodium, 50-100 million thousand units of streptomycin, 1-2g of levulose, 10-15ml of yolk and the balance of distilled water; and the diluent B is prepared by adding 12-16ml of glycerol or glycol into the 84-88 percent by volume of the diluent A used as a base solution. The preparation method mainly comprises the following steps of: weighting various raw materials and the distilled water for later use; preparing the base solution; preparing the diluent A; and preparing the diluent B. The diluent can be used for effectivlely reducing the bacterium content of the frozen cattle semens, is good in antibiotic effect, and can be used for greatly improving the frozen cattle sement production efficiency and the frozen cattle semen quality; and the preparation method is simple to operate and is economical and practical.

The invention discloses an application of phloretin in preparation of a pig, sheep or bovine semen cryopreservation agent. Meanwhile, the invention discloses a cryopreservation agent containing phloretin and a freezing base solution. According to the invention, phloretin is used for cryopreservation of semen of pigs, sheep and cattle for the first time; the application is reliable in effect, easyto popularize and suitable for artificial insemination of pig, sheep and cattle tubule frozen semen, long-term storage of the semen after ultralow-temperature freezing and semen deposition after long-distance transportation, the sperm motility rate, acrosome integrity rate and plasma membrane integrity rate of the frozen-thawed pig, sheep and cattle can be remarkably increased, and the fertilization capacity of sperms is kept.

The invention discloses a preparation method and a using method for GL and TCM199 serving as bovine semen diluent and bovine semen refrigerating fluid. The preparation method comprises preparation of the bovine semen diluent and preparation of the bovine semen refrigerating fluid. The using method comprises the steps of preparing the bovine semen diluent and preparing the bovine semen refrigerating fluid; collecting semen; performing preliminary dilution; performing secondary dilution; and performing refrigeration storage. According to the preparation method and the using method, the defreezing activity of frozen bovine semen is improved, the bovine artificial insemination conception rate is increased, the viscosity of the bovine semen diluent is reduced, and the granular size of the bovine semen diluent is lowered; the operation is simplified; the preparation method and the using method can be used under various large-scale production conditions; the standardization and practicability level of refrigeration of the bovine semen in various scales and the defreezing activity of bovine capillary refrigerated semen are improved; the aberration rate of the defrozen semen is lowered, and the bovine artificial insemination conception rate is increased.

The invention provides a protective agent for prolonging the vitality retention period of fresh bovine semen, which is prepared by mixing a mother liquor 1, a mother liquor 2 and fresh egg yolk liquid according to a certain proportion. Due to the adoption of the protective agent provided by the invention, the vitality of fresh bovine semen can still be maintained to be higher than 65% within 16 hours after semen collection, and the semen mortality of the fresh bovine semen is controlled to be 5% to 13% within 16 hours after semen collection. The results show that by using the bovine semen protective agent provided by the invention, the vitality retention period of the fresh bovine semen can be effectively prolonged, so that the long-distance transport is convenient, and a technical support and guarantee is provided for subsequent semen freezing treatment or sex-controlling separation treatment.

The invention provides a method for improving the sperm motility and increasing an in-vitro-fertilization rate of frozen bovine semen by the aid of genistein. The method is particularly characterized in that the genistein containing 1%o of DMSO (dimethyl sulfoxide) is added into SP-TALP (serum phosphate-total alkaline phosphatase) washing liquid for washing the frozen bovine semen in earlier-stage processing for the frozen bovine semen, or mixed liquid of PHE (phenylalanine) capacitation liquid and IVF-TALP (in vitro fertilization-total alkaline phosphatase) fertilization liquid containing bovine semen in a capacitation processing stage of second-stage pre-fertilization semen or prepared fertilization drops containing oocytes in a third-stage in-vitro-fertilization procedure; the added amounts of the genistein in two earlier stages of a fertilization procedure of each bottle of frozen bovine semen are 10 micromoles per liter, and the final concentration of the genistein in mixed liquid of the bovine semen and the fertilization drops containing the oocytes is 10 micromoles per liter when the genistein is added in a third stage of the fertilization procedure of each bottle of frozen bovine semen. The method has the advantages that the genistein is respectively used in the three in-vitro-fertilization stages, so that the sperm motility is greatly improved, and the sperm fertilization rate is greatly increased.

The invention discloses a bovine semen freezing dilute solution which contains a trace amount of rare-earth. The component of the bovine semen freezing dilute solution takes a commercially available cassou dilute solution as an application matrix and also contains the trace amount of rare-earth CeCl3 with the final concentration of 0.03-3 micromoles/liter. The invention discloses application of the bovine semen freezing dilute solution to cryopreservation of bovine semen. An experiment proves that the bovine semen freezing dilute solution disclosed by the invention accumulatively enhances five indexes for evaluating semen quality by 29% compared with a traditional freezing dilute solution; the bovine semen freezing dilute solution disclosed by the invention is easy to prepare, outstandingly superior to the traditional freezing dilute solution in total effect and worthy of popularization and application, and the source of the CeCl3 rare-earth guaranteed.

The invention discloses an oil tea meal polypeptide, and a preparation method and an application thereof in cryopreservation of bovine semen. The oil tea meal hydrolytic polypeptide has a variety of activities, and the activities of the hydrolytic polypeptide are affected by proteolytic sites in the oil tea meal: the polypeptide produced by trypsin hydrolysis and having the molecular weight of [5000 u, 3000 u] has the effect of improving the antioxidant ability of the bovine cryopreserved semen, and can be used as a bovine semen cryopreservation protection agent for reducing oxidative damage;and the polypeptide produced by alkaline protease hydrolysis and having the molecular weight of [5000 u, 3000 u] can promote empathema and pregnancy of anestrus cows. Moreover, the preparation methodof the hydrolytic polypeptide is simple, only simple crushing, enzymatic hydrolysis, ultrafiltration and freeze drying are used, and all the steps can be realized in a factory with mass production andare easy to implement. Waste reuse of camellia meiocarpa hu. meal is realized, and the economic added value of the camellia meiocarpa hu. meal can be increased.

The invention discloses camellia meal polypeptides, a preparation method and application in promoting oestrus and conception of anestrus cows. The hydrolyzed polypeptides of camellia meal have variousactivities, and the activities of the hydrolyzed polypeptides are affected by proteolytic sites in the camellia meal: the polypeptide which is produced by hydrolysis of trypsin and has a molecular weight of (5,000 mu, 3,000 mu) has the effect of improving the oxidation resistance performance of bovine cryopreserved semen, and can be used as a bovine semen cryoprotective agent to reduce oxidativeinjury; the polypeptide which is produced by hydrolysis of alkaline protease and has a molecular weight of (5,000 mu, 3,000 mu) can promote the oestrus and the conception of the anestrus cows. Furthermore, the preparation method of the hydrolyzed polypeptides is simple, and only simple smashing, enzymolysis, ultrafiltration and lyophilization are used. The steps all can realize mass production ina factory and are easy to implement. According to the camellia meal polypeptides, the preparation method and the application in promoting the oestrus and the conception of the anestrus cows, waste reuse of small-fruit camellia meal is realized, and the economical additional value of the small-fruit camellia meal can be increased.

The invention belongs to the technical field of preparation of genetic markers of cattle, and particularly relates to a genetic marker associated with semen quality traits, the marker is screened from a cattle FHL2 gene, the sequence of the marker is a 648bp-1170bp nucleotide sequence as shown in SEQ ID NO.1, T/G base mutation occurs at the 879th base r of the sequence, and FHL2 gene polymorphism is caused. The invention discloses an SNP typing detection technology of a promoter region of the bovine FHL2 gene, and finds that a T/G mutation site at the 879th basic group as shown in SEQ ID NO.1 is obviously related to the average sampling quantity character. The invention provides a new genetic marker for cattle reproductive trait marker-assisted selection.

The invention discloses a preparation method and a use method for EG (ethylene glycol) and TCM199 serving as bovine semen diluent and bovine semen freezing liquid. The preparation method for the EG and TCM199 serving as the bovine semen diluent and the bovine semen freezing liquid comprises the step of preparing the bovine semen diluent and the bovine semen freezing liquid. The use method for the EG and TCM199 serving as the bovine semen diluent and the bovine semen freezing liquid comprises the following steps of preparing the bovine semen diluent and the bovine semen freezing liquid; collecting semen; performing primary dilution; performing secondary dilution; performing freezing storage. According to the preparation method and the use method, the defreezing activity of frozen bovine semen is improved; the bovine artificial insemination conception rate is increased, the viscosity of the bovine semen diluent is lowered, and particles of the bovine semen diluent are reduced; a new-generation freezing agent with a small molecular weight and low toxicity is adopted to replace the original freezing agent with a large molecular weight and high toxicity; the operation is simplified; the preparation method and the use method are suitable for various types of large-scale production conditions.

The invention provides a process for preparing frozen bovine semen by using a frying and smoking method. The process comprises the following four steps: screening bovine semen, preparing a diluent, frying, and carrying out quality inspection. Compared with a traditional frozen beef refining preparation process, on one hand, the vitality of frozen beef sperms is improved by about 5%-8%, the vitality of original sperms can be effectively improved after freezing, the waste amount of the original sperms is reduced, and the production and preparation cost is reduced; and on the other hand, the production efficiency and the yield can be effectively improved, so that the consumption of diluent and thin tubes and the material loss are reduced, and meanwhile, the labor cost of production operationis effectively reduced.

The invention discloses a traditional Chinese medicine prescription capable of improving bull semen quality and a preparation method. The traditional Chinese medicine prescription contains the following raw materials in parts by weight: 30-40 g of herba epimedii, 30-40 g of semen cuscutae, 25-35 g of schisandra chinensis, 30-40 g of cynomorium songaricum, 25-35 g of cistanche, 15-25 g of fructus psoraleae, 5-10 g of cornu cervi pantotrichum, 10-15 g of lotus seeds, 20-25 g of fructus lycii, 5-20 g of cinnamon, 25-35 g of fructus cnidii, 25-35 g of prepared rehmannia roots, 30-40 g of semen allii tuberosi, 25-35 g of Chinese yam and 25-35 g of morinda officinalis. Compared with a control group, the seminal fluid volume of a self-formulated prescription group is obviously increased (p is less than 0.01), which indicates that the self-formulated prescription can increase the seminal fluid secretion volume of the bull with the kidney-yang deficiency syndrome; the semen density difference between the self-prepared prescription group and the estrus-promoting powder group is not obvious, which indicates that the self-prepared prescription and the estrus-promoting powder have no influence on the semen density of the bull with kidney-yang deficiency syndrome; compared with the self-prepared prescription group and the aphrodisiac powder group, the seminal fluid activity of the control group is obviously enhanced (p is less than 0.01), which indicates that the self-prepared prescription and the aphrodisiac powder can obviously improve the sperm activity of the bull with the kidney-yang deficiency syndrome.

The invention discloses a composition for detecting BVDV1 type in bovine semen, a kit and application, and belongs to the technical field of virus detection. The composition comprises a primer pair and a probe, the primer pair has nucleotide sequences as shown in SEQ ID NO: 1 and SEQ ID NO: 2, the probe has a nucleotide sequence as shown in SEQ ID NO: 3, the 5'end of the probe is connected with FAM, and the 3 'end of the probe is connected with BHQ1. On the basis of a micro-droplet digital PCR technology, the BVDV1 type in the bovine semen is specifically detected by using the primer and the probe, and the lowest detection limit can be as low as 1 copies/mu L; under a low copy number, compared with a conventional fluorescent quantitative PCR method, the detection microdroplet type digital PCR method provided by the invention is more sensitive, the generation of false negative is reduced, the specificity, sensitivity and absolute quantitative detection of the bovine BVDV1 type are realized, and a new detection method is provided for animal quarantine related detection departments and enterprises.