36 results about "Pinoresinol" patented technology

The invention discloses an effective part extract of valeriana amurensis P.Smirn. and a quality control method and medical use thereof. The content of total lignanoid substances in the extract accounts for over 50 percent of the total weight of the extract, wherein (+) pinoresinol-4,4'-O-beta-D-diglucopyranoside and (+) pinoresinol-8-O-beta-D-glucopyranoside are the main active components in the effective part extract, and the sum of the contents of the two active components accounts for more than 13 percent of the total weight of the extract. The invention also discloses a preparation method and the quality control method of the effective part extract. The effective part extract contains one effective part group of total lignanoid components and can effectively treat central nervous system degenerative diseases including senile dementia, tristimania and the like. The process for preparing the effective part extracts of the valeriana amurensis P.Smirn. for resisting the senile dementia can adapt to industrial production, and provides a new method for treating the central nervous system degenerative diseases by using the valeriana amurensis P.Smirn..

The present invention relates to a composition and method for preventing and treating cancer and modulating cell proliferation using compositions extracted from pinoresinol-rich Olea europaea Caiazzana olives. The present invention provides contacting one or more cells with a pharmaceutical effective amount of a polyphenolic composition isolated from Olea europaea Caiazzana olives. The polyphenolic composition includes one or more polyphenolic compounds extracted from Olea europaea Caiazzana olives.

Aiming at the phenomena that pinoresinol in the extraction process is easy to decompose to severely affect production of the pinoresinol on a large scale, the method provided by the invention separates and purifies pinoresinol and chlorogenic acid gradually by adopting ethanol extraction, rising film concentration, column chromatography and chromatographic separation, so that the requirements on production of the pinoresinol and chlorogenic acid on a large scale are realized, thereby effectively improving the yield of pinoresinol in eucommia leaves and meanwhile purifying high content chlorogenic acid.

The invention discloses a method for directly using samarium metal to promote the one-step reaction of vanillin and maleate in the presence of cuprous iodide and molecular sieves to prepare a dilactone-type pinoresin precursor. Under stirring, vanillin and maleate are mixed and dissolved in tetrahydrofuran that has been dehydrated and dried beforehand, and then samarium metal powder, cuprous iodide powder, and molecular sieve are added in sequence. The reaction system was carried out under absolutely anhydrous conditions. Stir at room temperature until the reaction is complete. The obtained reaction mixture is recovered by solvent, and after the residue is post-treated, it is separated by a chromatographic column to obtain a pure product of the dilactone-type pinoresin precursor.

The invention belongs to the field of medicine, and relates to application of lignan compounds in preparing an anti-hepatitis B virus medicine. According to the invention, the lignan compound 4-keto pinoresinol is extracted and isolated from Helicteres H.angustifolia, and upon tests, the lignan compound 4-keto pinoresinol is proved to have an anti-hepatitis B virus activity; by comparing the compound with lamivudine, which is taken as positive control, through a pharmacological test, a result shows that the compound is significant in anti-hepatitis B virus effect, and IC50 value to HBsAg is 36.6[micron]g/ml and IC50 value to HBeAg is 126.4[micro]g/ml, significantly greater than the positive control, namely the lamivudine; therefore, the compound disclosed by the invention can be used for preparing the anti-hepatitis B virus medicine as an active ingredient.

The invention provides application of pinoresinol in preparation of a medicine for inhibiting thrombosis, and relates to the field of chemical medicines. Specifically provided is the application of a compound shown by formula I, or a salt thereof, or a solvate thereof, or a hydrate thereof, or a prodrug thereof in the preparation of the medicine for inhibiting thrombosis. The compound shown by the formula I is preferably pinoresinol. Researches find that the compound pinoresinol extracted from t. maculata machride can be used for inhibiting vasoconstriction and has a remarkable effect of relaxing blood vessels. Meanwhile, the compound pinoresinol has an inhibiting effect on thrombosis, the thrombosis inhibiting effect of low-dose pinoresinol is better than that of high-dose pinoresinol, the thrombosis inhibiting rate is as high as 76.92%, the excellent thrombosis inhibiting effect is achieved while the medicine using dose is reduced, and the medicine using safety is improved. The compound pinoresinol can be used for preparing the medicine for inhibiting thrombosis, is used for preventing or treating thromboembolic diseases, and has a good application prospect.

The invention belongs to the field of traditional Chinese medicines, and particularly relates to a eucommia lignanoid preparation and a preparation method of the eucommia lignanoid preparation. The eucommia lignanoid preparation comprises the following raw materials in percentage by weight: 5%-20% of pinoresinol diglucoside, 5%-15% of syringaresinol diglucoside, 1%-10% of pinoresinol A diglucoside, 5%-15% of olivil biglucoside, and 1%-5% of naringenin B, wherein a eucommia lignanoid extract preferably contains 10%-15% of pinoresinol diglucoside, 8%-12% of syringaresinol diglucoside, 5%-8% of pinoresinol A diglucoside, 8%-12% of olivil biglucoside and 2%-4% of naringenin B.

The invention discloses a rosin furan preservative for dipping cotton wood and a preparation method and an application thereof. The preservative is mainly prepared from the following raw materials in parts by weight: 100 parts of pinoresinol solution and 20 to 30 parts of furan monomer, wherein the pinoresinol solution is prepared in the step of mixing 20 to 30 parts of rosin with 100 to 200 parts of organic solvents in parts by weight. The rosin furan preservative for dipping the cotton wood has the advantages of simplicity in preparation, low cost and no toxic effect; conventional high-pollution heavy-metal chemical preservation can be effectively replaced, the pollution is reduced, a preservative effect is good, and the durability of the cotton wood can be effectively increased; and by dipping in the rosin furan polymer preservative, the physical and chemical properties of the cotton wood can be changed, so that the cotton wood has the advantages of good corrosion resistant performance and good leach resistant performance and does not contain heavy metal, and the mothproof, antifungal and rotproof performance is improved.

The invention discloses a method for simultaneously determining the contents of eight index components in Miao medicine cynanchum auriculatum by a UPLC-ESI-MS (Ultra Performance Liquid Chromatography-ESI-Mass Spectrometry) method. According to the method, the contents of the eight index components in the Miao medicine cynanchum auriculatum are simultaneously determined by establishing the UPLC-ESI-MS method, wherein the eight index components comprise nodatin, pinoresinol, cynanchum bungeanum benzophenone, ferulic acid, gallic acid, scopoletin, syringic acid and chlorogenic acid. According to the method, an ACQUITYUPLCBEHC18 chromatographic column (2.1 mm*100 mm, 1.7 [mu]m) is adopted, a 0.1% formic acid aqueous solution and a 0.1% formic acid acetonitrile solution are adopted as a mobile phase, gradient elution is performed, the flow rate is 0.35 mL/min, the column temperature is 40 DEG C, and an electrospray ion source (ESI) and a multi-reaction ion monitoring (MRM) mode are adopted for detection. The method disclosed by the invention is simple, rapid, high in sensitivity and good in specificity, and can provide a reference basis for quality control of the cynanchum auriculatum medicinal material.

The invention provides a spiraea salicifolia stem and branch anti-rheumatoid-arthritis effective part and a preparation method and application thereof.According to the effective part, spiraea salicifolia stems and branches serve as raw materials, extract is obtained after 60% ethyl alcohol reflux extraction and ethyl alcohol pressure reduction recovery, the extract is purified with macroporous adsorption resin, and the total lignanoside effective part is obtained.The sum of weights of (+)-8'-hydroxy pinoresinol-8'-O-beta-d-glucopyranoside, (7R,8S)-dihydro dehydrogenation bis-coniferyl alcohol-9'-O-beta-d-glucopyranoside, (+)-lyonia ovalifolia tree ring lignans-9-O-beta-d-glucopyranoside and (+)-isolariciresinol-9-O-beta-d-pyran xyloside in the effective part ranges from 40% to 50%.The effective part is determined through the method that a modern separation means and pharmacological activity screening are combined, the ingredients of obtained effective part are clear, the content of active ingredients is high, it is shown through the in-vitro and in-vivo experiment results that the spiraea salicifolia stem and branch anti-rheumatoid-arthritis effective part inhibits generation of pro-inflammatory cytokine tumor necrosis factor-alpha, interleukin-1 beta and interleukin-6 to achieve the effect of treating rheumatoid arthritis.

The invention belongs to the technical field of medicines and in particular relates to a composition containing pinoresinol and cycloartenyl resin 9-glucoside as well as a preparation method and application of the composition. The composition is prepared from the following components: cycloartenyl resin 9-glucoside, chlorogenic acid, pinoresinol, eucommiol and quercetin. The composition provided by the invention can be used for effectively improving the effect of treating gonitis; meanwhile, the utilization rate of medicines is relatively high, and the cost is effectively reduced; and meanwhile, side effects of the medicines are reduced.

The invention discloses applications of pinoresinol in preparing products for preventing and treating diseases induced by oxidative stress. The chemical formula of pinoresinol is shown in the description, for the pinoresinol, by activating the Nrf2 signal channel, the protein levels of the antioxidant enzyme gamma GCS and the II phase detoxification enzyme NQO1 can be up-regulated, the GSH level of the endogenous antioxidant of cells is increased, and the cellular injuries induced by oxidative stress are inhibited; the pinoresinol has the similar cell protection effects for the human bronchusepithelial Beas-2B cells, the human renal glomerular endothelial cells HRGEC cells, the human nerve SHSY5Y cells and the human breast cancer MDA-MB-231 cells. The lignans can be used for preparing health products and medicines for preventing or treating diseases induced by oxidative stress.

The present invention provides a method for high-efficiency production of pinoresinol by use of an H₂O₂ auto-scavenging enzymatic cascade. It uses eugenol as the substrate, which is relatively inexpensive and is industrially available. It uses an enzymatic cascade to remove H₂O₂ produced in the process of pinoresinol synthesis, thereby reducing its inhibitory effect on the enzyme activity. In addition, the present invention uses whole cells as a catalyst, which can continuously regenerate cofactors needed by the enzyme, thus eliminating the need for exogenous addition of expensive cofactors during the reaction. The yield of the present invention can reach 7.12 g/L and the conversion rate is 61.55%.

The invention discloses a solenopsis invicta pheromone attractant and killing agent containing same, and relates to the technical field of pest control. The pheromone attractant is prepared from the following raw materials: 1 to 15 parts of linoleic acid, 1 to 15 parts of linolenic acid, 1 to 15 parts of arachidonic acid, 1 to 20 parts of omega-3 fatty acid, 1 to 15 parts of oleic acid, 1 to 10 parts of acetic acid, 1 to 15 parts of fatty glyceride, 1 to 15 parts of acetate, 1 to 15 parts of mannitol, 1 to 12 parts of sorbitol, 1 to 15 parts of butylene glycol, 1 to 15 parts of pinoresinol, 1 to 15 parts of dihydrocarveol, 1 to 15 parts of terpin, 1 to 50 parts of ethanol, and 1-20 parts of myrtenol. The killing agent further comprises a non-metal oxide and/or at least one of bacteria, fungi and insect protozoon. The solenopsis invicta pheromone attractant has the effects of solenopsis invicta food calling pheromone and solenopsis invicta tracing pheromone at the same time, the feeding amount of solenopsis invicta to the attractant is remarkably increased, the trapping and killing effect of the solenopsis invicta attractant is improved, and meanwhile the solenopsis invicta attractant has extremely high sensitivity and high specificity.

The invention discloses a method for generating pinoresinol and pinoresinol diglucoside through bioconversion of glucose by utilizing phomopsis. The method comprises the following steps: 1) preparing phomopsis into a frozen-dry thallus; 2) adding the frozen-dry thallus into sterilized water containing glucose, and shaking the flask containing the sterilized water for bioconversion reaction; 3) after conversion cultivation, carrying out centrifugation to remove the thallus, conducting vacuum concentration on the converted liquid, adding alcohol for alcohol precipitation, staying over, filtering out polyose and other impurities, and evaporating to obtain the dry pinoresinol diglucoside. According to the method, glucose serves as the only substrate, and pinoresinol and pinoresinol diglucoside can be generated under simple reaction conditions.

The invention discloses a method for preparing pinoresinol from salted turnips, which comprises the following steps: 1, the preparation of dry salted-turnip powder: slicing salted turnips, drying at a temperature of 50 DEG C, crushing, sieving by using a 20-mesh sieve so as to obtain dry salted-turnip powder, putting the dry salted-turnip powder into a sealed bag, and putting the sealed bag into a dryer for standby application; 2, the preparation of a salted-turnip crude extract; 3, the preliminary separation of the salted-turnip crude extract; 4, the separation and purification of a component Y1; and 5, the separation and purification of components 1-2: after a moderate amount of alcohol is added into the components 1-2 for dissolving, enabling the obtained object to pass through a reverse-phase silica gel column to carry out separation, eluting by using 50% CH3OH, and selecting a fourth flow component and separating by using a normal phase silica gel; eluting by using petroleum ether, collecting flow components of 97th to 116th pipes, merging the flow components, concentrating the obtained object, and removing a solvent by evaporating, so that pinoresinol is obtained. According to the invention, pinoresinol is separated from salted turnips for the first time, and as a main antifungal ingredient of the salted turnips, the pinoresinol is of great significance for the secondary development of salted turnips.