60 results about "Fresh semen" patented technology

The invention relates to a semen freezing method for sheep, in particular to an ultra-high activity tubule semen freezing method for sheep. The method solves the problems that after the conventional frozen semen of sheep thaws, the motility rate and the activity are low, and the frozen effect is unstable. The method comprises the following steps of: filling fresh semen of sheep into the bottom part of a first centrifuging tube holding cleaning liquid; standing in a CO2 incubator for 30 to 60 minutes, wherein the semen with high activity goes up in supernatant; sucking the supernatant and thenfilling into a second centrifuging tube for centrifugation, wherein the semen with high density and high activity is obtained at the bottom of the second centrifuging tube; and sucking the condensed high-activity semen, adding into a frozen diluent, uniformly mixing and filling in tubules; performing program freezing and storing in liquid nitrogen, and thawing the stored semen before semen deposition. The sheep semen is subjected to high activity screening before freezing according to the method, and the motility rate of the thawing tubule frozen semen of sheep reaches 0.45 to 0.60, requirements on the artificial fertilization of the frozen semen of sheep are fully met, and the method can be widely applied to mass production of sheep.

The invention relates to a method for preparing animal semen freeze-dried powder, which is used for preparing by adopting freeze-dried fresh semen and comprises the steps of prefreezing, sublimation drying and analysis drying. The invention has the following advantages that: (1) protein, microorganisms and the like can not denature or lose biological activity; (2) the loss of certain volatile components in the matter is small; (3) the growth of microorganisms and the action of enzymes are prohibited, so that the original nature of the freeze-dried powder can be maintained; (4) the volume is almost unchanged, the original structure is maintained, and the phenomena of concentration and hardening do not occur; (5) the dried matter is loose, porous and spongy, the dissolution is quick and complete after water addition, and the original nature can be recovered immediately; (6) certain easily oxidized substances in vacuum are protected; and (7) more than 95-99% of water can be removed by drying, and the dried product can stored for a long time without deterioration.

The invention discloses a frozen semen artificial insemination method for increasing the conception rate of donkeys. The method includes the steps of fresh semen freezing treatment, frozen semen unfreezing treatment and ovulation induction timing insemination. In the process of implementation, first, collected semen of the male donkeys is diluted and placed into a full-automatic freezing instrument to automatically complete freezing, wherein the motility rate is larger than or equal to 70%; before artificial insemination is performed on the female donkeys to be pregnant, the semen is rapidly placed into a water bath of 38 DEG C to be instantly unfrozen, and the step of ovulation induction timing insemination is completed within 1 hour. By the adoption of the method, the technical measure of ovulation induction timing insemination is adopted, so that the conception rate at estrus reaches 70%.

The invention provides a portable digital display electric heating constant-temperature semen collection cup for foxes. The portable digital display electric heating constant-temperature semen collection cup comprises a semen collection cup body, a heating device and a power source controller. The outer surface of the lower end of the semen collection cup body is provided with a displayer, a temperature adjusting button, a power switch and a charging jack; the upper end of the semen collection cup body is provided with a gland which can be connected with the semen collection cup body and a sealing cover connected with the gland; the bottom end of the inside of the semen collection cup body is provided with a spring; the inner wall of the semen collection cup body is provided with a groove, in which a temperature sensor is arranged; the upper end of a semen collection tube is conical in shape (shaped as a reversed cone frustum) and a protruding edge extends outward horizontally from the top end; an opening o the inner side of the upper end of the semen collection cup body is an inclined surface (similar to a side wall of a hopper body) matching the upper end of the semen collection tube; the upper end of the semen collection cup body is provided with a sunken groove matching the protruding edge. The portable digital display electric heating constant-temperature semen collection cup is convenient to carry and enables semen to be kept in a proper constant-temperature state in semen collection and fresh semen transport processes.

The invention relates to a semen freezing and diluting method capable of automatically adding liquid and adjusting the pre-freezing height, which comprises the following steps: putting fresh semen into a 50ml beaker, preheating the beaker on a constant-temperature water bath shaking table of an automatic liquid adding and diluting device, and setting the rotating speed of the shaking table to be 10-220rpm; controlling the dripping speed of the diluent to be 10-200 micro-liters per second; and during liquid nitrogen gas-phase pre-freezing, determining the height of a pre-freezing device according to animal varieties, and finally putting the pre-freezing device into liquid nitrogen for storage. According to the invention, diluent can be automatically added, standardized operation is realized, interference of personal manual operation is eliminated, and the liquid nitrogen gas phase pre-freezing height is adjusted to different values according to different animals. The device used in themethod is simple in structure, the device can be used after simple transformation by using existing instruments in a laboratory, and therefore the selling price of frozen semen cannot be affected, thequality of the frozen semen is remarkably improved, the lambing rate can be increased by 8%-9% in a production test, and the production benefits of livestock farms are remarkably improved.

The invention belongs to the technical field of pomegranate planting and particularly provides a cutting seedling-raising matrix for pomegranates. The cutting seedling-raising matrix is prepared from perlite, river sand, rotten leaf soil, fresh cow dung, compound fermentation bacteria, rice bran, soybean meal, fresh wax gourd peel, pepper leaves, fresh semen pharbitidis extract, litchi kernel extract, lignan, geniposidic acid and anaphalis xylorhiza alkali. The cutting seedling-raising matrix is comprehensive and sufficient in nutrition, good in permeability, strong in biological activity, good in slow-release property and long in acting time, does not need fertilization in a whole seedling-raising period, only needs watering, is simple to use, makes cuttings root quickly, makes stems and leaves grow healthily and strongly, improves the disease resistance ability of the cuttings, reduces plant diseases and insect pests and improves the seedling-raising quality of cuttings, and a rejuvenation period after transplantation is shortened. The cutting seedling-raising matrix makes the cutting rooting time shortened to be 13.6-14.1 days, the rooting rate is up to 97.8%-98.1%, and the survival rate is up to 96.3%-96.7%.

The invention relates to ultra-low temperature cryopreservation and recovery methods for the sperms of the sinocyclocheilus grahami, belonging to the technical field of cryobiology. The ultra-low temperature cryopreservation method comprises the following steps: selecting a manure high-quality protospecies or improved variety, anesthetizing for 5 minutes by 100ppm of MS-222, squeezing the dried abdomen to make the manure semen flow into a 1.5ml centrifuge tube, detecting the vitality of the fresh semen, freezing the fresh semen with the vitality of more than 85%, diluting the semen and the antifreeze liquid (D-15 and 7.5% ethylene glycol) according to the volume ratio of 1:2, subpackaging, balancing at 4 DEG C for 60 minutes, balancing in the position of 6cm above the liquid nitrogen surface for 2 minutes, balancing on the liquid nitrogen surface for 20 seconds and putting in the liquid nitrogen to preserve. The recovery method comprises the following steps: shaking the semen rapidly in the water bath of 37 DEG C to unfreeze, and mixing the sinocyclocheilus grahami sperms squeezed out with the unfreezed semen so that the ovums are fertilized. The methods have low cost, are easy and convenient to operate, ensure that the sperms of the sinocyclocheilus grahami can be preserved for a long time, the recovered sperms have high vitality and the artificially propagated sinocyclocheilus grahami has high fertilizing rate and hatching rate, can realize the large-scale production and are of great significance to the fish resource recovery and reconstruction.

The invention provides a processing technology of oolong tea, and relates to the field of processing of tea leaves. The processing technology comprises the following steps of selecting fresh leaves from tea trees, and performing spreading for air-drying on the fresh leaves; selecting fresh semen cassiae leafbuds, and ginkgo leafbuds, and adding withered tea leaves to a machine for rocking of greenleaves for making of green leaves; mixing the tea leaves with the semen cassiae leafbuds and ginkgo leafbuds to obtain a mixture, spreading the mixture for heat dissipation, putting the mixture afterheat dissipation in a rolling bucket, and performing rolling; controlling the room temperature during fermentation to be 22-24 DEG C, controlling the fermentation temperature of the tea leaves to be28 DEG C, controlling the humidity to be 90%, and controlling the fermentation time to be 4-5h; performing refrigeration drying treatment on the treated tea leaves; performing steam fixation treatment; and sending the treated tea leaves in a microwave fixation machine and performing fixation once again. According to the processing technology disclosed by the invention, running off of nutrients isavoided, low-pressure steam fixation is adopted, the tea leaves can absorb heat under the low-pressure steam environment, the condition that the leaves are maintained complete is facilitated, and fixation is performed for the second time after steam fixation to remove remaining moisture in the tea leaves. The semen cassiae leafbuds and the ginkgo leafbuds are added, so that the tea leaves are richer in mouth feel.

The invention relates to ultra-low temperature cryopreservation method for the sperms of the semilabeo obscurus, belonging to the technical field of cryobiology. The ultra-low temperature cryopreservation method comprises the following steps: selecting a manure high-quality protospecies or improved variety, anesthetizing for 5 minutes by 100ppm of MS-222, squeezing the dried abdomen slightly to make the manure semen flow into a 1.5ml centrifuge tube, detecting the vitality of the fresh semen, freezing the fresh semen with the vitality of more than 85%, diluting the collected high-quality semen and the antifreeze liquid (D-15 and 5% methanol) in three steps so that 1mL of the antifreeze liquid contains the number of the sperms of 2.5*108, filling into 1.8ml freezing tubes, balancing at 4 DEG C for 30 minutes, balancing in the position of 6cm above the liquid nitrogen surface for 10 minutes, balancing on the liquid nitrogen surface for 5 minutes, and putting in the liquid nitrogen to preserve. When in use, the preserved sperms are unfrozen in the water bath of 40 DEG C and are manfully fertilized. The method has low cost, is easy and convenient to operate, ensures that the sperms of the semilabeo obscurus can be preserved for a long time, the recovered sperms have high vitality and the manually propagated semilabeo obscurus has high fertilizing rate and hatching rate, can realize the large-scale production and are of great significance to the fish resource recovery and reconstruction.

The invention belongs to the technical field of livestock breeding, and particularly relates to an artificial breeding method for Limousin breeding cattle. The artificial breeding method includes thesteps of farm selection, farm arrangement, artificial feeding, hoof trimming, exercise controlling, grooming, weighing, trimming and sunstroke prevention in summer. A grain feed formula for the breeding cattle comprises 40% of corns, 20% of bran, 37% of bean pulp, 1% of macrovitamin, 1% of calcium hydrophosphate and 1% of salt. In summer, a breeder needs to ventilate and cool a cattle housing andshower the cattle thoroughly with clear water, and when the sunstroke of the cattle is found, the breeder needs to pull the cattle to a ventilated dry place and give the cattle heat-clearing and detoxifying injections and feed green bean soup. The artificial breeding method for the Limousin breeding cattle has the advantage that average weight, average height, average semen collection volume, average semen density, fresh semen activity and frozen semen activity of the bred adult cattle are obviously better than that of the cattle bred by a regular breeding method.

The invention provides a pig frozen semen basic diluent. The basic diluent comprises glucose, citric acid, cholesterol, L-proline, Tris and double distilled water. A frozen pig semen cryopreservationliquid further comprises the following components in percentage by volume: 20% of fresh yolk, 1*10<6> IU / L of penicillin, 1*10<6> IU / L of streptomycin, 3%-4% of glycerinum and 1%-2% of Equex.STM. A pig fresh-keeping semen diluent further comprises isotonic skimmed milk. According to the invention, the survival rate of local pig fresh semen in the long-distance transportation process can be effectively maintained, and the damage of pig sperms in the freezing process is prevented or significantly reduced by adding pig liver-derived cholesterol, L-proline and an antifreeze agent Equex.STM; and through an effective thawing method, the operation is convenient, and the sperm motility and quality are improved.

The invention discloses an experimental method for preserving a diluent of sheep semen under a room temperature. The experimental method comprises the following specific steps: 1) extracting the fresh sheep semen; 2) putting a semen collecting cup into a thermal insulation barrel and returning the semen collecting cup to a laboratory; 4) preparing four diluents; 5) putting the diluents into a 37-DEG C water-bath pot; 6) diluting the fresh semen; 7), storing diluted semen I, II, III and IV obtained in the step 6), tightly covering a centrifugal tube cover, putting the centrifugal tube on a foamed centrifugal tube rack, and placing and storing under the room temperature; 8), measuring a sperm motility rate in diluted semen; and 10), detecting a sperm plasma membrane integrity rate in the diluted semen. According to the experimental method for preserving the diluent of the sheep semen under the room temperature, disclosed by the invention, the semen with a relatively high survival rate is extracted, and influences, on experimental results, of a low sperm survival rate are reduced; and survival rates of sheep sperms are separately detected through the four groups of diluents with different proportions, and the measured diluent proportion of the diluent III is more beneficial for survival of the sperms.

The invention discloses a making method of goat fine tube frozen semen and belongs to the technical field of making of livestock and poultry frozen semen, in particular to a making method of goat fine tube frozen semen. The making method of the goat fine tube frozen semen comprises the following specific steps: 1, frozen diluent preparation; 2, semen collection and microscopic examination; 3, diluting; 4, sub-package; 5, balancing; 6, freezing; 7, unfreezing and re-inspection. The making method of the goat fine tube frozen semen, disclosed by the invention, is simple, convenient and quick to operate and requires no complex instrument and equipment; under the situation that the fresh semen quality meets the requirements, the motility rate of the frozen and unfrozen fine tube frozen semen is not lower than 0.35, so that not only can the requirements of artificial insemination for the survival rate of the frozen semen be met, but also the utilization rate of a goat can be improved, the limitation on animal lives is broken through, and the long-term preservation of the goat semen is realized; therefore, the application and the popularization of the frozen semen of the goat are prompted to a maximum limit.

The invention discloses a preparation method of frozen bovine semen diluting liquid. The preparation method comprises the following steps: weighing 5g to 8g of fructose and 19.8g to 26.6g of sodium citrate; adding 800mL of distilled water to stir and filtering a solution with filter paper into a conical flask or a saline solution bottle; putting into a water-bath pot at 75 DEG C to 80 DEG C and disinfecting for 30min; cooling to room temperature and adding 180mL to 220mL of fresh yolk; adding 0.4 to 0.5 million IU units of penicillin and 0.4 to 0.5 million IU units of streptomycin, wherein a solution prepared according to the ratio is named as a first solution; adding 430mL of the first solution into 65mL to 75mL of glycerol; after stirring, putting a mixture into a refrigerator at 3 DEG C to 5 DEG C to obtain a second solution; then diluting semen, freezing and packaging. By adopting the preparation method, a fresh semen diluting effect and the live rate of sperm of excellent breeding bulls are improved, and frozen semen is effectively preserved for a long period.

The invention discloses a preservation method of animal fresh semen and fresh semen preserving diluent specially used by the preservation method. The preservation method of the animal fresh semen, which is provided by the invention, comprises the following step of preserving the animal fresh semen by adopting the fresh semen preserving diluent; the fresh semen preserving diluent is prepared from 38 to 47mg / mL Tris, 510 to 640 units / mL penicillin, 580 to 710 units / mL streptomycin, 26 to 38mg / mL citric acid, 9 to 11mg / mL glucose, 0.35 to 0.42mg / mL vitamin B12 and 0.09 to 0.11mL / mL glycerinum. Experiments prove that by the method provided by the invention, the fresh semen of a breeding ram can be preserved for 24 to 96h, then insemination is performed on ewes, the conception rate of the ewes is between 83.5% and 86.2%, and the conception rate is not significantly different from the natural mating conception rate of the ewes. Therefore, by adopting the method provided the invention, the animal fresh semen can be preserved, and the preservation method has important application value.

The invention discloses a livestock semen normal-temperature preservative and a preparation method and a use method thereof. Every 100 mL of double distilled water contains the following components: 5-7 g of tris(hydroxyl)aminomethane (Tris), 1.6-2.0 g of sodium citrate, 0.4-0.5 g of vitamin C, 0.04-0.06 g of glutathione, 0.16-0.18 g of bovine serum albumin, 0.08-0.09 g of adenosine triphosphate,0.1-0.14 g of antibiotics, 5-20 ml of egg yolk and 0.5-2.5 g of trehalose. By using the livestock semen normal-temperature preservative, fresh semen can be stored at 15 to 25 DEG C, the cost of carrying refrigeration appliances such as a liquid nitrogen tank and a refrigerator is reduced, working efficiency of technicians is effectively improved, the preservation time is 72 hours or longer, the 72-hour survival rate is 45% or higher, the conception rate is 40% or higher after two times of continuous insemination, artificial insemination within the range of 300 kilometers can be guaranteed, thepreparation process is simple, the production cost is low, and large-scale application and popularization are facilitated.

The invention relates to nutritious coarse cereal flour. The nutritious coarse cereal flour is made from the following components by weight: 200g of fresh wheat kernels, 20g of fresh gordon euryale seeds, 20g of fresh haw seeds, 15g of fresh semen lablab album, 15g of fresh longan seeds and 20g of fresh lotus leaves. The flour disclosed by the invention contains multiple trace elements in coarse cereals, and through a unique processing method, the trace elements are easy to absorb by human bodies, the effects of promoting the metabolism of the human bodies, nourishing the brain and benefiting intelligence can be achieved, the economic benefits of the cereal grains are increased, the flour can be used as a raw material of cooked wheaten food products, and the flour is widely applied.

The invention relates to a method for preparing and unfreezing boar particle frozen semen. The steps of the method are of collecting and routinely examining boar semen, , preparing a diluent, diluting and balancing the semen, freezing and preserving the semen and unfreezing the frozen semen. The invention also relates to preparation of the diluent and a thawing solution. Compared with the prior art, the invention has the advantages that: after water-bath balancing, the fresh semen is directly centrifuged and no other diluents is needed to add, thereby reducing operation steps and reducing cost; the particle frozen semen is easy to operate and convenient to take; the thawing solution is easy to prepare and obtain and has the function of unfreezing and preserving the semen. The semen can survive at least for 420min after being unfrozen.

The invention relates to a preparation method of a hangover-nourishing liver tea, which belongs to the field of health care products. The present invention is aimed at the technical problems to be solved by the present invention on the market: there are stimulants in the rescue and nourishing liver tea on the market, and long-term use may cause hidden dangers of liver damage and elevated blood pressure, although the hangover sugar food can avoid the above-mentioned hidden dangers, but excessive intake of sugar will lead to diabetes and weight gain. The present invention uses the unique alkalis and amino acids in oolong tea, supplemented by fresh wolfberry, fresh cassia, fresh orange peel, sandalwood juice, honeysuckle , chrysanthemum and kudzu flower substances protect the liver, the beneficial substances are used as fermentation substrates, and the mycelia on the surface of fresh bean sprouts, sucrose, potassium dihydrogen phosphate, agar and waste mushroom medium are fermented, and the cultured mycelium is used as a fermentation attractant , to prepare beneficial enzyme substances, and then use sandalwood to adjust the smell, so that the smell of the produced enzyme substances is light and comfortable, so that the preparation has the effects of soothing the liver and detoxifying, strengthening the spleen and stomach, eliminating alcohol accumulation, lowering blood pressure and protecting the liver. The anti-alcoholic and liver-nourishing tea can be widely used in health care products and other fields.