42 results about "Erythroculter ilishaeformis" patented technology

The invention relates to a method of culturing Erythroculter ilishaeformis adult fishes. The method comprises the following steps: stocking the fish fries of Erythroculter ilishaeformis adult fishes in the water area of 5-10 mu with the density being 800-1000 /mu, and the specification of the fish fry stocking being 15-18 cm; providing a clean pond, rich water, water quality management, and the feeding of fish meals; controlling the water environment and the disease occurrences in the culturing process; and obtaining the Erythroculter ilishaeformis adult fishes with the average weight being 0.50kg/tail after one year of culture. Compared with the other inorganic and organic water fertilizing methods, the invention adopts the fertilizing method of using the zymotechnics of multiplicity of microbe groups, so as to reduce the pollution to the feeding environment of the pond by fertilizer water and achieve the purpose of emission reduction. In addition, photosynthetic bacteria are poured after the second day of the water fertilizing, so as to further increase the content of the photosynthetic bacteria in the pond, thus being favorable to the culturing of food organisms, decreasing the number of pathogens, and reducing the occurrence of various diseases.

The invention relates to a method of culturing Erythroculter ilishaeformis fish fries of large specifications. The method comprises the following steps: stocking the fish fries of Erythroculter ilishaeformis adult fishes in the water area which is relatively small and easily controlled with the reasonable density; providing rich water, stress management, and the feeding of fish meals; controlling the water environment and the disease occurrences in the culturing process; and obtaining the Erythroculter ilishaeformis fish fries with the average weight of each one being above 50g after 120-150 days of culture, and the specification being 15-18cm. Compared with the other inorganic and organic water fertilizing methods, the invention adopts the fertilizing method of using the zymotechnics of multiplicity of microbe groups, so as to reduce the pollution to the feeding environment of the pond by fertilizer water and achieve the purpose of emission reduction. In addition, the content of various beneficial bacterium groups in the pond is increased through the fertilizing method of using the zymotechnics of multiplicity of microbe groups, thus being favorable to the culturing of food organisms, thereby decreasing the number of pathogens, and reducing the occurrence of various diseases.

The invention relates to anti-stress expanded feed for Erythroculter ilishaeformis Bleeker and a manufacturing method thereof. The manufacturing method is characterized by comprising the following steps: firstly grinding fish meal, bean pulp, silkworm pupa, expanded soybeans, rapeseed meal, cottonseed meal, peanut meal, bran, wheat middling, squid extract and potassium sorbate mildew preventive; stirring and blending evenly with fish oil, vegetable oil, lecithin, 50% of choline chloride, rhubarb anthraquinones extractive, glutamine dipeptide, premix for fish, zeolite and monocalcium phosphate; grinding into mixed materials by an ultrafine grinding machine after stirring and blending evenly; moving the mixed material into a modulator and introducing water vapor for modulation; entering an extrusion pelleter to manufacture into feed particles after modulation; packaging the expanded feed particles after drying and cooling. The invention has simple preparation, balanced and rich nutrition, can satisfy the growth requirement of Erythroculter ilishaeformis Bleeker, improve fish body immunity and oxidation resistance, improve anti-stress and disease-resistant capabilities, reduce water body pollution caused by feed casting in the culturing process, improve feed palatability and promote growth.

The present invention relates to a processing method of Spanish mackerel, belonging to the field of fish fresh-keeping technology. Said processing method includes the following steps: soaking the pretreated fish body in a pickling liquor containing preservative, then drying and dewatering to obtain dried fish, then soaking the dried fish in a flavouring material liquor made up by using edible salt, monosodium glutamate, I+G, glutathione, white pepper, xanthoxylum, tea-polyphenol and water, further heating, drying to make its water content be 20-37%, then quickly cooling, shaping and vacuum packaging so as to obtain the invented product.

The invention relates to an erythroculter ilishaeformis spermatogonia stem cell separation and culture method. The method comprises the following steps: (1) collecting spermatic tissues: collecting spermary of erythroculter ilishaeformis with an age of seven months in a sterile environment, then rinsing spermary in PBS containing double-antibody, and finally grinding the spermary to disperse the spermary tissues; (2) digesting and separating spermatogonia stem cell: adding IV type collagenase (0.1%) into the dispersed spermary tissues, subjecting the digestive fluid to centrifugal separation, collecting the precipitate, digesting the precipitate by trypsin (0.25%), stopping the digestion by a culture medium containing FBS (10%), and collecting the cells; (3) carrying out primary culture of spermatogonia stem cell: using a DMEM/F12 complete medium containing cell factors to re-suspend the cells obtained in the step (2), adjusting the cell concentration, then paving the suspension liquid on a 24-hole cell culture plate coated by gelatin, and culturing the cells at a temperature of 26 DEG C. Trough the provided method, the in-vitro growth of primary cells of erythroculter ilishaeformis spermatogonia stem cell becomes easier, and an effective cell platform is provided for the research on reproduction and growth of erythroculter ilishaeformis.

The artificial Erythroculter ilishaeformis breeding and cultivating method includes mainly selecting parent fish, parent fish culture, artificially induced spawning, hatching, fry culture, summer fry culture, adult fish culture, large water surface culture and other steps. The method has the key technology of artificial propagation, domestication and bait conversion, etc. The present invention provides a method of cultivating Erythroculter ilishaeformis in large lake.

The invention relates to an erythroculter ilishaeformis special compound feed and a preparation method thereof. The erythroculter ilishaeformis special compound feed comprises the following components in parts by weight: 15-20 parts of fish meal, 15-20 parts of bean pulp, 15-20 parts of rapeseed meal, 15-20 parts of cottonseed meal, 15-20 parts of flour, 10-20 parts of rice bran, 0.5-2 parts of monocalcium phosphate, 0.1-0.5 part of multi-vitamin premix, 0.5-1 part of multi-mineral premix, 0.1-0.5 part of choline chloride, 0.2-0.5 part of table salt, 0.5-1.5 parts of soya-bean oil, 0.01-0.05 part of antioxidant, 0.1-0.5 part of mildew preventive, 5-10 parts of bentonite, 0.1-0.5 part of envelope lysine, 0.5-1 part of fennel, 0.1-0.5 part of ginger and 0.1-0.5 part of pine needle powder. The erythroculter ilishaeformis special compound feed can shorten the culture cycle of erythroculter ilishaeformis, improve the culture efficiency, promote the culture development and improve the meat quality of erythroculter ilishaeformis.

An artificial inducement method for spawning and breeding erythroculter ilishaeformis relates to an artificial inducement method for spawning and breeding fish. The method solves the problems of a low hatching rate and a low post-natal survival rate in the existing artificial reproduction method of the erythroculter ilishaeformis. The breeding method comprises the following steps: 1. culturing parent fish in a culture pond; 2. injecting an aphrodisiac agent into the parent fish twice so that the parent fish autogenously spawns; 3. transferring the fish eggs into a hatching barrel for hatching; and 4. transferring fish fry into an outdoor pond after opening an inflatable intestinal canal of a fish maw so as to culture young fishes. The artificial inducement method for spawning and breeding of the erythroculter ilishaeformis can help shorten a breeding period, greatly improve the survival rate and the hatching rate of the parent fish, and obviously improve the economic benefit.

The invention discloses a cultivation method of a gynogenesis megalobrama amblycephala, which comprises the following steps of: firstly selecting and breeding a female megalobrama amblycephala and a male erythroculter ilishaeformis bleeker as parent fishes; then carrying out artificial induced spawning to obtain the sperms of the erythroculter ilishaeformis bleeker and the ripe ovums of the megalobrama amblycephala; mixing the erythroculter ilishaeformis bleeker sperms inactivated by ultraviolet rays with the ripe ovums of the megalobrama amblycephala; 3-4 minutes later, placing the ovums activated at 0-4 DEG C for cold shock treatment for 20-30 minutes; and after incubating embryos treated by cold shock at 23-24 DEG C, obtaining diploid gynogenesis megalobrama amblycephalas through a flow type cell DNA content determination method and a chromosome ploidy detection method of peripheral blood cell culturing. The cultivation method of the gynogenesis megalobrama amblycephala has the advantages of short cultivation time, fast growth speed, high incubation rate, excellent offspring property, and the like, and has great importance on aspects of fish inheritance breeding and biological evolution researches.

The invention discloses a subfamily distant hybridization method for Xenocypris davidi Bleeker and Erythroculter ilishaeformis Bleeker. The method comprises the following steps: by using Xenocypris davidi Bleeker as a female parent fish and Erythroculter ilishaeformis Bleeker as a male parent fish, firstly carrying out artificial induced spawning on the female parent fish and the male parent fish, carrying out artificial dry-method insemination on the female parent fish and the male parent fish which have good induced spawning effects, incubating with flowing water in a culture dish after insemination, farming after fries are incubated, and obtaining filial generations of Xenocypris davidi bleeker and Erythroculter ilishaeformis Bleeker by detection after breeding. The subfamily distant hybridization method provided by the invention has the advantages of simple steps, convenient operation, high fertility rate and hatching rate, good characters for later generations and the like.

The invention discloses a breeding method for distant hybridization between erythroculter ilishaeformis and ancherythropterus nigrocauda. The method includes the following steps: 1) selecting selectively-bred erythroculter ilishaeformis and selectively-bred ancherythropterus nigrocauda as parents; 2) conducting parents strengthening breeding including erythroculter ilishaeformis strengthening breeding and ancherythropterus nigrocauda strengthening breeding; 3) conducting artificial spawning induction: respectively injecting spawning induction medicine hypophysis cerebri or dopamine antagonist DOM, lutein releasing hormone analogue and chorionic gonadotropin which are used singly or in mixing mode on the two parents; 4) conducting artificial semen and spawn collection and insemination including dry method insemination and semi-dry method insemination; and 5) conducting artificial incubation. Hybridized culter 'vanguard 1' obtained by distant hybridization has the advantages of good body type and large size of the erythroculter ilishaeformis, also has the advantages of low breeding cost, mild temper, easiness in catching and easiness in live marketing of the ancherythropterus nigrocauda and further has excellent hybridization performances like high growing speed.

The invention discloses an artificial breeding method for erythroculter ilishaeformis and belongs to the technical field of aquaculturing. In the artificial breeding method for erythroculter ilishaeformis, natural stocking of erythroculter ilishaeformis can be gradually changed into artificial breeding through controls on feeding to the erythroculter ilishaeformis of each domestication stage during the growth of the erythroculter ilishaeformis. The breeding method comprises twice disinfection of the culturing pond before the erythroculter ilishaeformis is placed in a culturing pond. During the domestication and culturing, various feeding methods and fodders are employed for the erythroculter ilishaeformis during each growth phases according to life habits of the erythroculter ilishaeformis and digestive physiology of the same, so the erythroculter ilishaeformis can be domesticated. The method is suitable for artificial breeding for erythroculter ilishaeformis.

The invention discloses an establishment method and a fry culture method of a heterologous hybrid culter strain. The establishment method comprises the steps that erythroculter ilishaeformis in the Changjiang river system serves as a female parent, 'Pujiang No.1' megalobrama amblycephala serves as a male parent, artificial induced spawning, fertilization, hatching, fry culture and screening are carried out, and hermaphroditic fertile diploid hybrid culter F1 is obtained; the hybrid culter F1 and the male parent erythroculter ilishaeformis in the Changjiang river system are subjected to backcross, hatching and breeding are carried out, diploid hybrid culter F2 is obtained, and establishment of the heterologous hybrid culter strain is completed; the diploid hybrid culter F2 is subjected to self-cross, screening is carried out, hybrid culter F3 is obtained, hybrid culter F4 and hybrid culter F5 are obtained in the same way, and the stable heterologous hybrid culter strain is obtained. Compared with the prior art, by means of the establishment method of the heterologous hybrid culter strain, the novel hybrid fish, namely heterologous hybrid culter, which has the hybrid advantages of a high growing speed, tightly-closed scales, stable hypoxia resistance inheritance, hermaphroditic fertility and the like can be cultured, and great significance is achieved on the aspects of fish genetic breeding and biological evolution.

The invention relates to special feed for intercropping yellow-head catfish and erythroculter ilishaeformis. The special feed comprises following components by weight: fish meal, corn flour, soybean pulp, peanut powder, soybean meal, cottonseed, lecithin, flour, brown sugar, compound vitamin, a compound mineral substance, methionine, ferric sulfate, copper sulfate, zinc sulfate and manganese sulfate. According to the special feed, the components are mixed uniformly according to the ratio, the fish meal, the corn flour, the flour, and the like are used as the main raw materials, the brown sugar, the compound vitamin, the compound mineral substance and the sulfates are added, and the components are mixed with water according to a certain ratio, smashed and granulated into particles having a common particle size to feed fish. After being fed, the two kinds of fish grow fast, the conversion rate of the feed is high, the food coefficient is 1.4-1.5, the disease resistance and anti-stress capability of the two kinds of fish are significantly enhanced, and the final output is high.

The invention provides a use method of a suspended line fishing gear for solving the technical difficulty problem that a great amount of fish can not be fished at the determined depth in a deep water region in the prior art. The method comprises the following steps: at least two support points are arranged in a prefishing region; a line fishing gear is arranged between the two support points; a fish line group is arranged on a main line of the line fishing gear; the main lines at the two ends of the fish line group are respectively provided with a lower drop block which is connected with the corresponding support point through the main line; and the line is released for fishing through regulating the main line. The fish line group can be freely regulated in water; living edible fishes, such as mandarin fish, catfish and erythroculter ilishaeformis and the like can be fished in mass; and fishing amount for each time is reliable.

The invention discloses a molecular breeding method for thickening intermuscular thorns of erythroculter ilishaeformis and megalobrama amblycephala, and belongs to the technical field of aquatic organism breeding. According to the molecular breeding method, based on a technical means of gene editing, the F0 generation of targeted mutation erythroculter ilishaeformis and megalobrama amblycephala mstn genes is obtained, and mutant individuals with mstn deletion and increased intermuscular bony area are obtained through passage. According to the invention, a parent method for obtaining erythroculter ilishaeformis and megalobrama amblycephala with increased intermuscular stabbing area by utilizing a gene editing technology is provided for the first time. The method is beneficial to large-scalecultivation of wild erythroculter ilishaeformis and megalobrama amblycephala which have thick intermuscular thorns and can be inherited in production. Being different from a transgenic method, the method can be applied to artificial breeding, overcomes the difficulty that intermuscular thorns are small and difficult to process in production, does not need to worry about the influence of transgenic food on people, is convenient for people to find the intermuscular thorns more easily when people eat erythroculter ilishaeformis and megalobrama amblycephala, and is easy to popularize in production.

The invention discloses a method for salting erythroculter ilishaeformis and relates to the technical field of food processing. The method comprises the following steps: pretreating raw materials, preparing spices, salting, air-drying, performing vacuum package, performing code printing, and the like. According to the technical scheme of the invention, the traditional fish salting technology is combined with sanitation and safety requirements of modern food processing and the requirement on human healthy diet on the basis of a lot of tests by an applicant, the salted erythroculter ilishaeformis is obtained by adopting scientific blending and modern equipment processing, and the problems that the traditional salted fish is high in salt content and turns yellow and generates rancidity in the storage period due to oxidation are effectively solved. Moreover, the salted erythroculter ilishaeformis processed by using the technology is mellow in fish flavor, rich in salting flavor and firm and elastic in meat quality and meets the low-salt, healthy and delicious requirements on diet of modern people.

The invention discloses a low salinity mixed culture method of Pelteobagrus fulvidraco. A stocking method includes: stocking Erythroculter ilishaeformis under 800/mu in late February, stocking Pelteobagrus fulvidraco under 500/mu in middle March, stocking Micropterus salmoides under 300/mu in middle May, stocking Pelteobagrus fulvidraco under 200/min in early June, fishing with netting from late July by capturing large fishes and omitting small fishes until all are captured for sales in December. Compared with the prior art, the invention has the following advantages: the method is reasonably planned, pond space may be fully utilized, corresponding nutrition may be supplemented just by consuming residual feed of Erythroculter ilishaeformis or Micropterus salmoides according to the feature that Pelteobagrus fulvidraco is omnivorous, disease resistance of the fishes cultured is improved and a water changing period is prolonged through improving the water quality and using low salinity pond water, multiple fishing may reduce pond stock rate and facilitate culturing management so that the stocked fishes can grow fast and high specification is obtained, the economic value is high, and culturing cost is saved.

The invention relates to a net screen used for breeding and catching fishes and shrimps. The net cage includes a cylindrical steel framework which is surrounded by a polyethylene net, the polyethylene net on the lateral side of the steel framework is provided with an inlet connected to a conical passage; erythroculter ilishaeformis is bred in the net cage, and meanwhile, some trash fishes and shrimps can be caught for supplying natural baits to erythroculter ilishaeformis.

The invention discloses an erythroculter ilishaeformis deoxyribonucleic acid (DNA) bar code sequence and application thereof, and belongs to the technical field of species identification. The erythroculter ilishaeformis DNA bar code sequence is as shown in SEQ ID NO:1, and is an erythroculter ilishaeformis COI (cytochrome c oxidase subunit I) gene. The DNA bar code sequence can be used as a standard test sequence for erythroculter ilishaeformis DNA and can be used for effectively identifying erythroculter ilishaeformis species.

The invention discloses a distant hybridization method for grass carp and an erythroculter ilishaeformis subfamily. The method comprises the following steps that the grass carp are adopted as female parent fishes, erythroculter ilishaeformis is adopdted as a male parent fish, the female parent fishes and the male parent fish are subjected to artificial hasten parturition in a breeding season, drymethod artificial insemination is carried out, and obtained germ cells are subjected to lotic hatching in a hatching trough; after fry are demoulded, all the fry hatch and point waists appear, farmingis carried out, detecting and screening are carried out, and diploid hybrid fishes and triploid hybrid fishes of the grass carp and the erythroculter ilishaeformis are obtained. According to the hybridization method, the advantages of herbivority, high growth speed and the slim figure of the female parent grass carp and the advantages of tender meat quality, high stress resisting and disease resisting ability, high growth speed and the like of the male parent erythroculter ilishaeformis are combined, the diploid hybrid fishes and the triploid hybrid fishes with obvious hybridization advantages are creatively cultivated, precious resources are provided for forming of subsequent novel grass carp strains, and important biological significance and economic value are achieved in the aspect offish genetic breeding and production application.

The present invention relates to an antibacterial peptide derived from Erythroculter ilishaeformis and use thereof. The amino acid sequence of the antibacterial peptide of the present invention includes an amino acid sequence as shown in SEQ ID NO: 1. The present invention further discloses use of the antibacterial peptide in the preparation of an anti-aquatic-bacterium drug for aquaculture animals. The present invention provides a novel antibacterial peptide, discloses a function of the novel antibacterial peptide for resisting aquatic bacteria, and provides candidate molecules for preventing and treating bacterial diseases in the culture process of Erythroculter ilishaeformis.